Restriction map for
pFN21AB9414
(Circular) (Six-base) MAPSORT of: pFN21AB9414.seq Check: 6195 from: 1 to: 6844
pFN21AB9414 6844 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4654 278
Size: 53 83 186 936 3118 2468
Fragments arranged by size:
3118 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4187 1937
Size: 2250 4594
Acc65I G'GTAC_C
Cuts at: 4165 4165
Size: 6844
AclI AA'CG_TT
Cuts at: 1563 4971 5344 1563
Size: 3408 373 3063
Fragments arranged by size:
3408 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2744 2826 3296 3843 3902 3967 4069 4907
Size: 1112 82 470 547 59 65 102 838
Cuts at: 4907 6291 6559 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1112 838 547 470 268 102
82 65 59
AfeI AGC'GCT
Cuts at: 3949 3949
Size: 6844
AflII C'TTAA_G
Cuts at: 820 1017 2561 820
Size: 197 1544 5103
Fragments arranged by size:
5103 1544 197
AflIII A'CryG_T
Cuts at: 1203 2314 5743 6720 1203
Size: 1111 3429 977 1327
Fragments arranged by size:
3429 1327 1111 977
AgeI A'CCGG_T
Cuts at: 5675 5675
Size: 6844
AhdI GACnn_n'nnGTC
Cuts at: 5573 5573
Size: 6844
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6844
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3390 1184
Size: 2206 4638
AlwNI CAG_nnn'CTG
Cuts at: 3093 3356 4587 6159 3093
Size: 263 1231 1572 3778
Fragments arranged by size:
3778 1572 1231 263
ApaLI G'TGCA_C
Cuts at: 2967 3762 4901 6057 2967
Size: 795 1139 1156 3754
Fragments arranged by size:
3754 1156 1139 795
ApoI r'AATT_y
Cuts at: 1493 2136 4153 4379 4531 6640 1493
Size: 643 2017 226 152 2109 1697
Fragments arranged by size:
2109 2017 1697 643 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5398 160
Size: 872 4366 1606
Fragments arranged by size:
4366 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6844
AvaI C'yCGr_G
Cuts at: 1870 1943 4169 1870
Size: 73 2226 4545
Fragments arranged by size:
4545 2226 73
BamHI G'GATC_C
Cuts at: 3618 4174 4541 3618
Size: 556 367 5921
Fragments arranged by size:
5921 556 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4165 5620 618
Size: 325 217 627 2378 1455 1842
Fragments arranged by size:
2378 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 4163 1391
Size: 673 2099 4072
Fragments arranged by size:
4072 2099 673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2196 2511 3849 928
Size: 971 138 159 315 1338 3923
Fragments arranged by size:
3923 1338 971 315 159 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2120 2154 5035 5069 1903
Size: 34 183 34 2881 34 3678
Fragments arranged by size:
3678 2881 183 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4736 5946 4736
Size: 1210 5634
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6844
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5455 136
Size: 107 122 71 631 605 3783 1525
Fragments arranged by size:
3783 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6839 6839
Size: 6844
BlpI GC'TnA_GC
Cuts at: 4261 4261
Size: 6844
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2971 3691 3766 4905 6061 1790
Size: 258 923 720 75 1139 1156 2573
Fragments arranged by size:
2573 1156 1139 923 720 258 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6844
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3137 4575 5533
Size: 416 53 83 399 24 1720 1438 958
Cuts at: 5533 442
Size: 1753
Fragments arranged by size:
1753 1720 1438 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6844
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3450 3450
Size: 6844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5504 1239
Size: 4265 2579
Bpu10I CC'TnA_GC
Cuts at: 3247 3683 3247
Size: 436 6408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4969 5834 6132 4969
Size: 865 298 5681
Fragments arranged by size:
5681 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5507 882
Size: 617 4008 2219
Fragments arranged by size:
4008 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2315 4635 493
Size: 711 1111 2320 2702
Fragments arranged by size:
2702 2320 1111 711
BsaBI GATnn'nnATC
Cuts at: 3891 6838 3891
Size: 2947 3897
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4651 5033 5668 275
Size: 53 83 186 936 3118 382 635 1451
Fragments arranged by size:
3118 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2496 5277 5675 5949 6096 6507 2496
Size: 2781 398 274 147 411 2833
Fragments arranged by size:
2833 2781 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6619
Fragments arranged by size:
6619 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3310 4020 1186
Size: 2124 710 4010
Fragments arranged by size:
4010 2124 710
BseYI C'CCAG_C
Cuts at: 2194 2877 3068 3829 3944 4094 6047 6543 2194
Size: 683 191 761 115 150 1953 496 2495
Fragments arranged by size:
2495 1953 761 683 496 191 150 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3986 1163
Size: 573 2250 4021
Fragments arranged by size:
4021 2250 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4294 5055 6083 1540
Size: 275 186 2293 761 1028 2301
Fragments arranged by size:
2301 2293 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2971 3766 4905 4990 6061 2971
Size: 795 1139 85 1071 3754
Fragments arranged by size:
3754 1139 1071 795 85
BsmI GAATG_Cn'
Cuts at: 4355 4448 4355
Size: 93 6751
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2064 2971 3691 3766 4163 4905
Size: 399 258 16 907 720 75 397 742
Cuts at: 4905 4990 6061 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 907 742 720 399 397 258
85 75 16
BspEI T'CCGG_A
Cuts at: 6507 6507
Size: 6844
BspHI T'CATG_A
Cuts at: 2644 4733 6463 2644
Size: 2089 1730 3025
Fragments arranged by size:
3025 2089 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4199 844
Size: 3355 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3455 4731 1394
Size: 429 1632 1276 3507
Fragments arranged by size:
3507 1632 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5339 5513 65
Size: 1183 4091 174 1396
Fragments arranged by size:
4091 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5488 5675 1700
Size: 110 3678 187 2869
Fragments arranged by size:
3678 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6844
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6844
BssSI C'ACGA_G
Cuts at: 4904 5916 4904
Size: 1012 5832
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6844
BstBI TT'CG_AA
Cuts at: 5648 5648
Size: 6844
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6844
BstYI r'GATC_y
Cuts at: 1972 3618 3993 4174 4541 4926 4943 6395 6481
Size: 1646 375 181 367 385 17 1452 86
Cuts at: 6481 6493 6839 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1646 1452 385 375 367 346 181
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6105
Fragments arranged by size:
6105 683 56
BtsI GCAGTG_nn'
Cuts at: 2621 2807 3777 4360 5154 5174 2621
Size: 186 970 583 794 20 4291
Fragments arranged by size:
4291 970 794 583 186 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6844
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2901 2936 3700 3735 529
Size: 35 2337 35 764 35 3638
Fragments arranged by size:
3638 2337 764 35 35 35
DraI TTT'AAA
Cuts at: 2382 3657 4148 2382
Size: 1275 491 5078
Fragments arranged by size:
5078 1275 491
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5851 809
Size: 507 4535 1802
Fragments arranged by size:
4535 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2664 4291 5180
Size: 54 1425 50 275 18 834 1627 889
Cuts at: 5180 6809 8
Size: 1629 43
Fragments arranged by size:
1629 1627 1425 889 834 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4291 1537
Size: 275 2479 4090
Fragments arranged by size:
4090 2479 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2217 3128 3315 3328 3885 4774 1353
Size: 864 911 187 13 557 889 3423
Fragments arranged by size:
3423 911 889 864 557 187 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5431 5805 5951 387
Size: 1064 138 3842 374 146 1280
Fragments arranged by size:
3842 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2744 2826 3296 3843 3902 3967 4069
Size: 393 1112 82 470 547 59 65 102
Cuts at: 4069 4907 5504 6291 6559 1239
Size: 838 597 787 268 1524
Fragments arranged by size:
1524 1112 838 787 597 547 470 393
268 102 82 65 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2982 2982
Size: 6844
EcoO109I rG'GnC_Cy
Cuts at: 3922 3922
Size: 6844
EcoRI G'AATT_C
Cuts at: 2136 4153 4531 2136
Size: 2017 378 4449
Fragments arranged by size:
4449 2017 378
EcoRV GAT'ATC
Cuts at: 2080 2886 2080
Size: 806 6038
FspI TGC'GCA
Cuts at: 5350 5350
Size: 6844
HaeII r_GCGC'y
Cuts at: 1678 2362 3951 3978 5991 1678
Size: 684 1589 27 2013 2531
Fragments arranged by size:
2531 2013 1589 684 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2990 3022 5485 5517 5559
Size: 32 135 32 1418 32 2463 32 42
Cuts at: 5559 5591 1373
Size: 32 2626
Fragments arranged by size:
2626 2463 1418 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 4188 4434 669
Size: 988 281 595 1655 246 3079
Fragments arranged by size:
3079 1655 988 595 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6844
HpaI GTT'AAC
Cuts at: 4434 4434
Size: 6844
KpnI G_GTAC'C
Cuts at: 4169 4169
Size: 6844
MfeI C'AATT_G
Cuts at: 4443 4443
Size: 6844
MluI A'CGCG_T
Cuts at: 6720 6720
Size: 6844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3771 3798 3995 5958 6142 1305
Size: 2466 27 197 1963 184 2007
Fragments arranged by size:
2466 2007 1963 197 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5022
Fragments arranged by size:
5022 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3932 4802 5161 5320 518
Size: 648 2766 870 359 159 2042
Fragments arranged by size:
2766 2042 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3819 4000 4260 4937 6085 6330 1606
Size: 2213 181 260 677 1148 245 2120
Fragments arranged by size:
2213 2120 1148 677 260 245 181
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6292
NdeI CA'TA_TG
Cuts at: 387 2449 387
Size: 2062 4782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6844
NotI GC'GGCC_GC
Cuts at: 4291 4291
Size: 6844
NspI r_CATG'y
Cuts at: 1132 1229 2172 2638 4202 5747 1132
Size: 97 943 466 1564 1545 2229
Fragments arranged by size:
2229 1564 1545 943 466 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6844
PciI A'CATG_T
Cuts at: 5743 5743
Size: 6844
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2915 3093 3356 1108
Size: 1807 178 263 4596
Fragments arranged by size:
4596 1807 263 178
PmeI GTTT'AAAC
Cuts at: 4148 4148
Size: 6844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4913 4945 6426 6458 4913
Size: 32 1481 32 5299
Fragments arranged by size:
5299 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6844
PsiI TTA'TAA
Cuts at: 2205 3462 4414 2205
Size: 1257 952 4635
Fragments arranged by size:
4635 1257 952
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2706 2738 2706
Size: 32 6812
PstI C_TGCA'G
Cuts at: 830 2594 2814 3379 4196 830
Size: 1764 220 565 817 3478
Fragments arranged by size:
3478 1764 817 565 220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6844
PvuII CAG'CTG
Cuts at: 3819 3819
Size: 6844
SalI G'TCGA_C
Cuts at: 1936 4186 1936
Size: 2250 4594
SapI GCTCTTCn'nnn_
Cuts at: 2217 2217
Size: 6844
SbfI CC_TGCA'GG
Cuts at: 4196 4196
Size: 6844
ScaI AGT'ACT
Cuts at: 5092 6804 5092
Size: 1712 5132
SexAI A'CCwGG_T
Cuts at: 2914 2914
Size: 6844
SfcI C'TryA_G
Cuts at: 826 1045 2590 2810 3099 3156 3375 3624 3645
Size: 219 1545 220 289 57 219 249 21
Cuts at: 3645 4118 4192 5327 6008 6199 826
Size: 473 74 1135 681 191 1471
Fragments arranged by size:
1545 1471 1135 681 473 289 249 220
219 219 191 74 57 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6844
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6844
SmaI CCC'GGG
Cuts at: 1872 4171 1872
Size: 2299 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2561 4948 5849 6111 820
Size: 197 926 618 2387 901 262 1553
Fragments arranged by size:
2387 1553 926 901 618 262 197
SnaBI TAC'GTA
Cuts at: 493 4635 493
Size: 4142 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6844
SphI G_CATG'C
Cuts at: 1132 4202 1132
Size: 3070 3774
SspI AAT'ATT
Cuts at: 5 52 2396 4768 5
Size: 47 2344 2372 2081
Fragments arranged by size:
2372 2344 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6844
StyI C'CwwG_G
Cuts at: 513 1065 2064 3500 4283 513
Size: 552 999 1436 783 3074
Fragments arranged by size:
3074 1436 999 783 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4871 4888 5041 5226 5671 325
Size: 1255 3291 17 153 185 445 1498
Fragments arranged by size:
3291 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5090 6802 96
Size: 274 80 33 51 1443 3113 1712 138
Fragments arranged by size:
3113 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3832 3864 6804 6836 3832
Size: 32 2940 32 3840
Fragments arranged by size:
3840 2940 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6844
XbaI T'CTAG_A
Cuts at: 4180 4180
Size: 6844
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6844
XmaI C'CCGG_G
Cuts at: 1870 4169 1870
Size: 2299 4545
XmnI GAAnn'nnTTC
Cuts at: 4973 4973
Size: 6844
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4652 276
Size: 53 83 186 936 3118 2468
Fragments arranged by size:
3118 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI