Restriction map for
pFN21AB9405
(Circular) (Six-base) MAPSORT of: pFN21AB9405.seq Check: 2533 from: 1 to: 6217
pFN21AB9405 6217 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2673 4280 5664 5932 1632
Size: 1041 1607 1384 268 1917
Fragments arranged by size:
1917 1607 1384 1041 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 3463 4946 3463
Size: 1483 4734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2345 2935 3960 5532 2345
Size: 590 1025 1572 3030
Fragments arranged by size:
3030 1572 1025 590
ApaI G_GGCC'C
Cuts at: 2090 2090
Size: 6217
ApaLI G'TGCA_C
Cuts at: 2168 4274 5430 2168
Size: 2106 1156 2955
Fragments arranged by size:
2955 2106 1156
ApoI r'AATT_y
Cuts at: 1493 2739 3019 3526 3752 3904 6013 1493
Size: 1246 280 507 226 152 2109 1697
Fragments arranged by size:
2109 1697 1246 507 280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2102 3107 3183 3542 1870
Size: 73 69 90 1005 76 359 4545
Fragments arranged by size:
4545 1005 359 90 76 73 69
BamHI G'GATC_C
Cuts at: 2008 3547 3914 2008
Size: 1539 367 4311
Fragments arranged by size:
4311 1539 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 2134 2143 3170 3538
Size: 325 217 627 326 21 9 1027 368
Cuts at: 3538 4993 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1027 627 368 326 325 217
21 9
BanII G_rGCy'C
Cuts at: 1391 2090 2307 2356 2380 2873 3536 1391
Size: 699 217 49 24 493 663 4072
Fragments arranged by size:
4072 699 663 493 217 49 24
BbeI G_GCGC'C
Cuts at: 2138 2138
Size: 6217
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2484 3093 928
Size: 971 585 609 4052
Fragments arranged by size:
4052 971 609 585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3268 4109 5319 3268
Size: 841 1210 4166
Fragments arranged by size:
4166 1210 841
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2263 4828 136
Size: 107 122 71 631 605 591 2565 1525
Fragments arranged by size:
2565 1525 631 605 591 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2475 3634 2475
Size: 1159 5058
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2172 2247 2259 4278 5434 1790
Size: 300 82 75 12 2019 1156 2573
Fragments arranged by size:
2573 2019 1156 300 82 75 12
BmgBI CAC'GTC
Cuts at: 1318 2173 1318
Size: 855 5362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3948 4906 442
Size: 416 53 83 399 24 2531 958 1753
Fragments arranged by size:
2531 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2373 2460 2820 3089 4877 1239
Size: 980 154 87 360 269 1788 2579
Fragments arranged by size:
2579 1788 980 360 269 154 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2183 4880 882
Size: 617 684 2697 2219
Fragments arranged by size:
2697 2219 684 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2135 2883 4024 4406
Size: 53 83 186 936 602 748 1141 382
Cuts at: 4406 5041 275
Size: 635 1451
Fragments arranged by size:
1451 1141 936 748 635 602 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2096 2268 4650 5048 5322 5469 5880 2096
Size: 172 2382 398 274 147 411 2433
Fragments arranged by size:
2433 2382 411 398 274 172 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3422 1186
Size: 2236 3981
BseYI C'CCAG_C
Cuts at: 2455 3008 5420 5916 2455
Size: 553 2412 496 2756
Fragments arranged by size:
2756 2412 553 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 1163
Size: 573 948 4696
Fragments arranged by size:
4696 948 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2380 2836 4278 4363 5434 2172
Size: 208 456 1442 85 1071 2955
Fragments arranged by size:
2955 1442 1071 456 208 85
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2172 2247 2259 2307 2356 2380
Size: 399 300 82 75 12 48 49 24
Cuts at: 2380 2836 2873 3536 4278 4363 5434 1391
Size: 456 37 663 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 663 456 399 300 85
82 75 49 48 37 24 12
BspEI T'CCGG_A
Cuts at: 2096 2268 5880 2096
Size: 172 3612 2433
Fragments arranged by size:
3612 2433 172
BspHI T'CATG_A
Cuts at: 2220 4106 5836 2220
Size: 1886 1730 2601
Fragments arranged by size:
2601 1886 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2519 3572 844
Size: 1675 1053 3489
Fragments arranged by size:
3489 1675 1053
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2997 4712 4886 65
Size: 1183 1749 1715 174 1396
Fragments arranged by size:
1749 1715 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 2110 4861 5048 1700
Size: 110 225 75 2751 187 2869
Fragments arranged by size:
2869 2751 225 187 110 75
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2345 2920 2935 2977 2992 794
Size: 1551 575 15 42 15 4019
Fragments arranged by size:
4019 1551 575 42 15 15
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 2532 2532
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 2008 3151 3547 3914 4299 4316 5768 5854
Size: 36 1143 396 367 385 17 1452 86
Cuts at: 5854 5866 6212 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1143 396 385 367 346 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 2176 513
Size: 552 939 114 58 4554
Fragments arranged by size:
4554 939 552 114 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2343 3733 4527 4547 2343
Size: 1390 794 20 4013
Fragments arranged by size:
4013 1390 794 20
ClaI AT'CG_AT
Cuts at: 1554 2583 1554
Size: 1029 5188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 3664 4553
Size: 54 1425 50 275 18 405 1429 889
Cuts at: 4553 6182 8
Size: 1629 43
Fragments arranged by size:
1629 1429 1425 889 405 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2703 4147 1353
Size: 860 490 1444 3423
Fragments arranged by size:
3423 1444 860 490
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 5178 5324 387
Size: 1064 138 3215 374 146 1280
Fragments arranged by size:
3215 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2378 2378
Size: 6217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2373 2460 2673 2820 3089 4280
Size: 393 587 154 87 213 147 269 1191
Cuts at: 4280 4877 5664 5932 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1191 787 597 587 393 269 268
213 154 147 87
EcoO109I rG'GnC_Cy
Cuts at: 2086 2605 2693 2086
Size: 519 88 5610
Fragments arranged by size:
5610 519 88
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2996 2996
Size: 6217
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2138 2207 5364 1678
Size: 460 69 3157 2531
Fragments arranged by size:
3157 2531 460 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 2809 2841 3076
Size: 32 135 32 722 32 483 32 235
Cuts at: 3076 3108 3406 3438 3449 3481 4858 4890 4932
Size: 32 298 32 11 32 1377 32 42
Cuts at: 4932 4964 1373
Size: 32 2626
Fragments arranged by size:
2626 1377 722 483 298 235 135 42
32 32 32 32 32 32 32 32
32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3432 3499 748
Size: 2684 67 3466
Fragments arranged by size:
3466 2684 67
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2134 2134
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2928 5331 5515 1305
Size: 1623 2403 184 2007
Fragments arranged by size:
2403 2007 1623 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4395
Fragments arranged by size:
4395 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4175 4534 4693 518
Size: 648 3009 359 159 2042
Fragments arranged by size:
3009 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2178 3633 4310 5458 5703 1606
Size: 514 58 1455 677 1148 245 2120
Fragments arranged by size:
2120 1455 1148 677 514 245 58
NarI GG'CG_CC
Cuts at: 2135 2135
Size: 6217
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4726
Fragments arranged by size:
4726 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2372 3575 5120 1132
Size: 97 1143 1203 1545 2229
Fragments arranged by size:
2229 1545 1203 1143 97
PasI CC'CwG_GG
Cuts at: 1348 2044 1348
Size: 696 5521
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2775 1108
Size: 1667 4550
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2605 2693 2605
Size: 88 6129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2086 2086
Size: 6217
PstI C_TGCA'G
Cuts at: 830 2350 2403 2931 2940 3429 3569 830
Size: 1520 53 528 9 489 140 3478
Fragments arranged by size:
3478 1520 528 489 140 53 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
SacI G_AGCT'C
Cuts at: 2380 2380
Size: 6217
SacII CC_GC'GG
Cuts at: 2121 2179 2121
Size: 58 6159
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SapI GCTCTTCn'nnn_
Cuts at: 2213 2213
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 2332 4465 6177 2332
Size: 2133 1712 2372
Fragments arranged by size:
2372 2133 1712
SfcI C'TryA_G
Cuts at: 826 1045 2346 2399 2927 2936 3223 3425 3565
Size: 219 1301 53 528 9 287 202 140
Cuts at: 3565 4700 5381 5572 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1301 1135 681 528 287 219 202
191 140 53 9
SfoI GGC'GCC
Cuts at: 2136 2136
Size: 6217
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 2014 2104 3109 3544 1872
Size: 142 90 1005 435 4545
Fragments arranged by size:
4545 1005 435 142 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3183 4321 5222 5484 820
Size: 197 926 1240 1138 901 262 1553
Fragments arranged by size:
1553 1240 1138 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2004 2050 2786 3367 3656 513
Size: 552 939 46 736 581 289 3074
Fragments arranged by size:
3074 939 736 581 552 289 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 2482 2879 4244 4261 4414 4599
Size: 1255 627 275 397 1365 17 153 185
Cuts at: 4599 5044 325
Size: 445 1498
Fragments arranged by size:
1498 1365 1255 627 445 397 275 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2330 3194 4463
Size: 274 80 33 51 1443 353 864 1269
Cuts at: 4463 6175 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1269 864 353 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 3183 1943
Size: 1240 4977
XmaI C'CCGG_G
Cuts at: 1870 2012 2102 3107 3542 1870
Size: 142 90 1005 435 4545
Fragments arranged by size:
4545 1005 435 142 90
XmnI GAAnn'nnTTC
Cuts at: 2979 3323 3345 3501 4346 2979
Size: 344 22 156 845 4850
Fragments arranged by size:
4850 845 344 156 22
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SanDI SexAI
SfiI SrfI SwaI XcmI