Restriction map for
pFN21AB9403
(Circular) (Six-base) MAPSORT of: pFN21AB9403.seq Check: 3603 from: 1 to: 8545
pFN21AB9403 8545 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5800 5800
Size: 8545
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4101 6355 278
Size: 53 83 186 936 2565 2254 2468
Fragments arranged by size:
2565 2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5888 1937
Size: 3951 4594
Acc65I G'GTAC_C
Cuts at: 3255 5055 5631 5866 3255
Size: 1800 576 235 5934
Fragments arranged by size:
5934 1800 576 235
AclI AA'CG_TT
Cuts at: 1563 6672 7045 1563
Size: 5109 373 3063
Fragments arranged by size:
5109 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2791 2909 3557 4019 4085 4379 4406 4492
Size: 1159 118 648 462 66 294 27 86
Cuts at: 4492 4721 6608 7992 8260 1632
Size: 229 1887 1384 268 1917
Fragments arranged by size:
1917 1887 1384 1159 648 462 294 268
229 118 86 66 27
AfeI AGC'GCT
Cuts at: 2742 3952 5576 2742
Size: 1210 1624 5711
Fragments arranged by size:
5711 1624 1210
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8348
AflIII A'CryG_T
Cuts at: 1203 7444 8421 1203
Size: 6241 977 1327
Fragments arranged by size:
6241 1327 977
AgeI A'CCGG_T
Cuts at: 7376 7376
Size: 8545
AhdI GACnn_n'nnGTC
Cuts at: 7274 7274
Size: 8545
AleI CACnn'nnGTG
Cuts at: 1166 4880 1166
Size: 3714 4831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2669 3707 1184
Size: 1485 1038 6022
Fragments arranged by size:
6022 1485 1038
AlwNI CAG_nnn'CTG
Cuts at: 2872 4396 5065 5416 5831 6288 7860 2872
Size: 1524 669 351 415 457 1572 3557
Fragments arranged by size:
3557 1572 1524 669 457 415 351
ApaI G_GGCC'C
Cuts at: 3346 5045 3346
Size: 1699 6846
ApaLI G'TGCA_C
Cuts at: 2723 6602 7758 2723
Size: 3879 1156 3510
Fragments arranged by size:
3879 3510 1156
ApoI r'AATT_y
Cuts at: 1493 2309 4512 5854 6080 6232 8341 1493
Size: 816 2203 1342 226 152 2109 1697
Fragments arranged by size:
2203 2109 1697 1342 816 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7099 160
Size: 872 6067 1606
Fragments arranged by size:
6067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8545
AvaI C'yCGr_G
Cuts at: 1870 1943 2164 2639 3463 5870 1870
Size: 73 221 475 824 2407 4545
Fragments arranged by size:
4545 2407 824 475 221 73
AvrII C'CTAG_G
Cuts at: 5029 5029
Size: 8545
BamHI G'GATC_C
Cuts at: 5875 6242 5875
Size: 367 8178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 3255 3575 3700 4552
Size: 325 217 627 438 1030 320 125 852
Cuts at: 4552 5055 5631 5866 7321 618
Size: 503 576 235 1455 1842
Fragments arranged by size:
1842 1455 1030 852 627 576 503 438
325 320 235 217 125
BanII G_rGCy'C
Cuts at: 1391 2157 2771 3346 3473 4165 4314 5045 5082
Size: 766 614 575 127 692 149 731 37
Cuts at: 5082 5864 1391
Size: 782 4072
Fragments arranged by size:
4072 782 766 731 692 614 575 149
127 37
BbeI G_GCGC'C
Cuts at: 2229 2229
Size: 8545
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 3359 3757 4155 928
Size: 971 190 1270 398 398 5318
Fragments arranged by size:
5318 1270 971 398 398 190
BbvCI CC'TCA_GC
Cuts at: 4715 5003 5351 4715
Size: 288 348 7909
Fragments arranged by size:
7909 348 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2116 2150 3116 3150 5644 5678 6736
Size: 34 179 34 966 34 2494 34 1058
Cuts at: 6736 6770 1903
Size: 34 3678
Fragments arranged by size:
3678 2494 1058 966 179 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6437 7647 6437
Size: 1210 7335
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2088 3168 5789
Size: 107 122 71 631 605 416 1080 2621
Cuts at: 5789 7156 136
Size: 1367 1525
Fragments arranged by size:
2621 1525 1367 1080 631 605 416 122
107 71
BglII A'GATC_T
Cuts at: 4453 5371 8540 4453
Size: 918 3169 4458
Fragments arranged by size:
4458 3169 918
BlpI GC'TnA_GC
Cuts at: 4308 5962 4308
Size: 1654 6891
Bme1580I G_kGCm'C
Cuts at: 1790 2727 3346 3705 3871 5045 6606 7762 1790
Size: 937 619 359 166 1174 1561 1156 2573
Fragments arranged by size:
2573 1561 1174 1156 937 619 359 166
BmgBI CAC'GTC
Cuts at: 1318 5094 1318
Size: 3776 4769
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3343 3951 4614
Size: 416 53 83 399 24 1926 608 663
Cuts at: 4614 5318 6276 7234 442
Size: 704 958 958 1753
Fragments arranged by size:
1926 1753 958 958 704 663 608 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4328
Size: 8545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2280 2438 3098 3977 4031 4121 4220 4300
Size: 1041 158 660 879 54 90 99 80
Cuts at: 4300 4673 5087 5276 5388 5525 7205 1239
Size: 373 414 189 112 137 1680 2579
Fragments arranged by size:
2579 1680 1041 879 660 414 373 189
158 137 112 99 90 80 54
Bpu10I CC'TnA_GC
Cuts at: 2058 4715 4989 5003 5351 5571 2058
Size: 2657 274 14 348 220 5032
Fragments arranged by size:
5032 2657 348 274 220 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4034 4877 5496 5633 6670 7535 7833 4034
Size: 843 619 137 1037 865 298 4746
Fragments arranged by size:
4746 1037 865 843 619 298 137
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2475 3114 4227 4914 5153 7208 882
Size: 617 976 639 1113 687 239 2055 2219
Fragments arranged by size:
2219 2055 1113 976 687 639 617 239
BsaAI yAC'GTr
Cuts at: 493 1204 2665 4687 5581 5842 6336 493
Size: 711 1461 2022 894 261 494 2702
Fragments arranged by size:
2702 2022 1461 894 711 494 261
BsaBI GATnn'nnATC
Cuts at: 8539 8539
Size: 8545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 4098 6352 6734
Size: 53 83 186 936 693 1872 2254 382
Cuts at: 6734 7369 275
Size: 635 1451
Fragments arranged by size:
2254 1872 1451 936 693 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3093 3817 4537 5139 6978 7376 7650 7797 8208
Size: 724 720 602 1839 398 274 147 411
Cuts at: 8208 3093
Size: 3430
Fragments arranged by size:
3430 1839 724 720 602 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3896 3926 4955 4985 5107
Size: 30 165 30 2493 30 1029 30 122
Cuts at: 5107 5137 5192 5222 1178
Size: 30 55 30 4501
Fragments arranged by size:
4501 2493 1029 165 122 55 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2234 3143 3463 3841 3844 5261 5468
Size: 1033 15 909 320 378 3 1417 207
Cuts at: 5468 5630 1186
Size: 162 4101
Fragments arranged by size:
4101 1417 1033 909 378 320 207 162
15 3
BseYI C'CCAG_C
Cuts at: 2199 2399 2657 3847 4242 4671 4710 4930 5019
Size: 200 258 1190 395 429 39 220 89
Cuts at: 5019 5606 5796 7748 8244 2199
Size: 587 190 1952 496 2500
Fragments arranged by size:
2500 1952 1190 587 496 429 395 258
220 200 190 89 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2107 2519 2847 5262 5495 1163
Size: 573 371 412 328 2415 233 4213
Fragments arranged by size:
4213 2415 573 412 371 328 233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2882 4973 5360 5995 6756
Size: 275 186 39 842 2091 387 635 761
Cuts at: 6756 7784 1540
Size: 1028 2301
Fragments arranged by size:
2301 2091 1028 842 761 635 387 275
186 39
BsiHKAI G_wGCw'C
Cuts at: 2727 3333 3452 4314 6606 6691 7762 2727
Size: 606 119 862 2292 85 1071 3510
Fragments arranged by size:
3510 2292 1071 862 606 119 85
BsiWI C'GTAC_G
Cuts at: 5838 5838
Size: 8545
BsmI GAATG_Cn'
Cuts at: 2264 3213 6056 6149 2264
Size: 949 2843 93 4660
Fragments arranged by size:
4660 2843 949 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2727 2771 3333 3346 3452 3473
Size: 399 367 570 44 562 13 106 21
Cuts at: 3473 3705 3871 4165 4314 5045 5082 5864 6606
Size: 232 166 294 149 731 37 782 742
Cuts at: 6606 6691 7762 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 782 742 731 570 562 399
367 294 232 166 149 106 85 44
37 21 13
BspEI T'CCGG_A
Cuts at: 4537 5139 8208 4537
Size: 602 3069 4874
Fragments arranged by size:
4874 3069 602
BspHI T'CATG_A
Cuts at: 2953 6434 8164 2953
Size: 3481 1730 3334
Fragments arranged by size:
3481 3334 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 3501 5043 5415 5800 5900 844
Size: 1352 1305 1542 372 385 100 3489
Fragments arranged by size:
3489 1542 1352 1305 385 372 100
BsrBI CCG'CTC
Cuts at: 1394 1823 2181 2914 3708 5362 6432 1394
Size: 429 358 733 794 1654 1070 3507
Fragments arranged by size:
3507 1654 1070 794 733 429 358
BsrDI GCAATG_nn'
Cuts at: 65 1248 5118 7040 7214 65
Size: 1183 3870 1922 174 1396
Fragments arranged by size:
3870 1922 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5355 7189 7376 1700
Size: 110 3545 1834 187 2869
Fragments arranged by size:
3545 2869 1834 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8545
BssSI C'ACGA_G
Cuts at: 6605 7617 6605
Size: 1012 7533
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4276 4396 5482 794
Size: 3482 120 1086 3857
Fragments arranged by size:
3857 3482 1086 120
BstBI TT'CG_AA
Cuts at: 2007 7349 2007
Size: 5342 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8545
BstXI CCAn_nnnn'nTGG
Cuts at: 2471 3401 4333 2471
Size: 930 932 6683
Fragments arranged by size:
6683 932 930
BstYI r'GATC_y
Cuts at: 1972 4021 4453 5371 5590 5649 5875 6242 6627
Size: 2049 432 918 219 59 226 367 385
Cuts at: 6627 6644 8096 8182 8194 8540 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2049 1977 1452 918 432 385 367 346
226 219 86 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 2685 5698 2685
Size: 3013 5532
BtgI C'CryG_G
Cuts at: 513 1065 2022 2194 2394 2514 3924 513
Size: 552 957 172 200 120 1410 5134
Fragments arranged by size:
5134 1410 957 552 200 172 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7806
Fragments arranged by size:
7806 683 56
BtsI GCAGTG_nn'
Cuts at: 4476 4943 5832 6061 6855 6875 4476
Size: 467 889 229 794 20 6146
Fragments arranged by size:
6146 889 794 467 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8510
DraI TTT'AAA
Cuts at: 5849 5849
Size: 8545
DraIII CAC_nnn'GTG
Cuts at: 3582 3582
Size: 8545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4097 7552 809
Size: 507 2781 3455 1802
Fragments arranged by size:
3455 2781 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2962 3004
Size: 54 1425 50 275 18 259 873 42
Cuts at: 3004 3403 3779 5357 5992 6881 8510 8
Size: 399 376 1578 635 889 1629 43
Fragments arranged by size:
1629 1578 1425 889 873 635 399 376
275 259 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 5357 5992 1537
Size: 275 3545 635 4090
Fragments arranged by size:
4090 3545 635 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2471 2885 3444 3546 4408 4670 5592
Size: 966 152 414 559 102 862 262 922
Cuts at: 5592 6475 1353
Size: 883 3423
Fragments arranged by size:
3423 966 922 883 862 559 414 262
152 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7132 7506 7652 387
Size: 1064 138 5543 374 146 1280
Fragments arranged by size:
5543 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4312 4312
Size: 8545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2280 2438 2791 2909 3098 3557 3977
Size: 393 648 158 353 118 189 459 420
Cuts at: 3977 4019 4031 4085 4121 4220 4300 4379 4406
Size: 42 12 54 36 99 80 79 27
Cuts at: 4406 4492 4673 4721 5087 5276 5388 5525 6608
Size: 86 181 48 366 189 112 137 1083
Cuts at: 6608 7205 7992 8260 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1083 787 648 597 459 420 393
366 353 268 189 189 181 158 137
118 112 99 86 80 79 54 48
42 36 27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3747 4049 3747
Size: 302 8243
EcoO109I rG'GnC_Cy
Cuts at: 2796 2972 3070 3342 3398 3752 4042 4132 4288
Size: 176 98 272 56 354 290 90 156
Cuts at: 4288 4791 4888 5042 5281 2796
Size: 503 97 154 239 6060
Fragments arranged by size:
6060 503 354 290 272 239 176 156
154 98 97 90 56
EcoRI G'AATT_C
Cuts at: 2309 4512 5854 6232 2309
Size: 2203 1342 378 4622
Fragments arranged by size:
4622 2203 1342 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5191 5212 5191
Size: 21 8524
FspI TGC'GCA
Cuts at: 3516 7051 3516
Size: 3535 5010
HaeII r_GCGC'y
Cuts at: 1678 2229 2744 3559 3610 3641 3954 5206 5578
Size: 551 515 815 51 31 313 1252 372
Cuts at: 5578 7692 1678
Size: 2114 2531
Fragments arranged by size:
2531 2114 1252 815 551 515 372 313
51 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2424 2456 2730
Size: 32 135 32 479 32 341 32 274
Cuts at: 2730 2762 2977 3009 3373 3405 3896 3928 4955
Size: 32 215 32 364 32 491 32 1027
Cuts at: 4955 4987 5363 5395 5546 5578 7186 7218 7260
Size: 32 376 32 151 32 1608 32 42
Cuts at: 7260 7292 1373
Size: 32 2626
Fragments arranged by size:
2626 1608 1027 491 479 376 364 341
274 215 151 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 3022 5889 6135 669
Size: 988 281 223 861 2867 246 3079
Fragments arranged by size:
3079 2867 988 861 281 246 223
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8545
HpaI GTT'AAC
Cuts at: 6135 6135
Size: 8545
KasI G'GCGC_C
Cuts at: 2225 2225
Size: 8545
KpnI G_GTAC'C
Cuts at: 3259 5059 5635 5870 3259
Size: 1800 576 235 5934
Fragments arranged by size:
5934 1800 576 235
MfeI C'AATT_G
Cuts at: 6144 6144
Size: 8545
MluI A'CGCG_T
Cuts at: 8421 8421
Size: 8545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4344 5478 7659 7843 1305
Size: 3039 1134 2181 184 2007
Fragments arranged by size:
3039 2181 2007 1134 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3006 3405 10
Size: 54 1425 343 1174 399 5150
Fragments arranged by size:
5150 1425 1174 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 2958 3570 3716 4331 4880 6503
Size: 648 1566 226 612 146 615 549 1623
Cuts at: 6503 6862 7021 518
Size: 359 159 2042
Fragments arranged by size:
2042 1623 1566 648 615 612 549 359
226 159 146
MspA1I CmG'CkG
Cuts at: 1606 2121 2196 2765 3262 3997 4384 4675 4714
Size: 515 75 569 497 735 387 291 39
Cuts at: 4714 5007 5254 5740 5961 6638 7786 8031 1606
Size: 293 247 486 221 677 1148 245 2120
Fragments arranged by size:
2120 1148 735 677 569 515 497 486
387 293 291 247 245 221 75 39
NaeI GCC'GGC
Cuts at: 5357 5357
Size: 8545
NarI GG'CG_CC
Cuts at: 2226 2226
Size: 8545
NcoI C'CATG_G
Cuts at: 513 1065 2022 3924 513
Size: 552 957 1902 5134
Fragments arranged by size:
5134 1902 957 552
NdeI CA'TA_TG
Cuts at: 387 2340 3223 387
Size: 1953 883 5709
Fragments arranged by size:
5709 1953 883
NgoMIV G'CCGG_C
Cuts at: 5355 5355
Size: 8545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8545
NotI GC'GGCC_GC
Cuts at: 5992 5992
Size: 8545
NspI r_CATG'y
Cuts at: 1132 1229 3694 5680 5903 7448 1132
Size: 97 2465 1986 223 1545 2229
Fragments arranged by size:
2465 2229 1986 1545 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8545
PciI A'CATG_T
Cuts at: 7444 7444
Size: 8545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4144 4249 1108
Size: 3036 105 5404
Fragments arranged by size:
5404 3036 105
PfoI T'CCnGG_A
Cuts at: 4137 4428 4137
Size: 291 8254
PmeI GTTT'AAAC
Cuts at: 5849 5849
Size: 8545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6614 6646 8127 8159 6614
Size: 32 1481 32 7000
Fragments arranged by size:
7000 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2796 2972 3070 3398 3752 4042 4132 4288 2796
Size: 176 98 328 354 290 90 156 7053
Fragments arranged by size:
7053 354 328 290 176 156 98 90
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8545
PsiI TTA'TAA
Cuts at: 6115 6115
Size: 8545
PspOMI G'GGCC_C
Cuts at: 3342 5041 3342
Size: 1699 6846
PstI C_TGCA'G
Cuts at: 830 4284 4401 4483 5412 5625 5745 5897 830
Size: 3454 117 82 929 213 120 152 3478
Fragments arranged by size:
3478 3454 929 213 152 120 117 82
PvuI CG_AT'CG
Cuts at: 2001 2882 2001
Size: 881 7664
PvuII CAG'CTG
Cuts at: 3997 4384 4675 4714 5254 5740 3997
Size: 387 291 39 540 486 6802
Fragments arranged by size:
6802 540 486 387 291 39
SacI G_AGCT'C
Cuts at: 4314 4314
Size: 8545
SacII CC_GC'GG
Cuts at: 2197 2197
Size: 8545
SalI G'TCGA_C
Cuts at: 1936 5887 1936
Size: 3951 4594
SanDI GG'GwC_CC
Cuts at: 2972 3070 2972
Size: 98 8447
SapI GCTCTTCn'nnn_
Cuts at: 2319 3546 4408 5592 2319
Size: 1227 862 1184 5272
Fragments arranged by size:
5272 1227 1184 862
SbfI CC_TGCA'GG
Cuts at: 4401 5897 4401
Size: 1496 7049
ScaI AGT'ACT
Cuts at: 6793 8505 6793
Size: 1712 6833
SfcI C'TryA_G
Cuts at: 826 1045 4280 4397 4479 4810 5408 5621 5687
Size: 219 3235 117 82 331 598 213 66
Cuts at: 5687 5741 5893 7028 7709 7900 826
Size: 54 152 1135 681 191 1471
Fragments arranged by size:
3235 1471 1135 681 598 331 219 213
191 152 117 82 66 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 3168 5789 3168
Size: 2621 5924
SfoI GGC'GCC
Cuts at: 2227 2227
Size: 8545
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8545
SmaI CCC'GGG
Cuts at: 1872 5872 1872
Size: 4000 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4049 4892 5511 5612 6649 7550
Size: 197 926 2106 843 619 101 1037 901
Cuts at: 7550 7812 820
Size: 262 1553
Fragments arranged by size:
2106 1553 1037 926 901 843 619 262
197 101
SnaBI TAC'GTA
Cuts at: 493 5842 6336 493
Size: 5349 494 2702
Fragments arranged by size:
5349 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8545
SphI G_CATG'C
Cuts at: 1132 3694 5903 1132
Size: 2562 2209 3774
Fragments arranged by size:
3774 2562 2209
SspI AAT'ATT
Cuts at: 5 52 2708 6469 5
Size: 47 2656 3761 2081
Fragments arranged by size:
3761 2656 2081 47
StuI AGG'CCT
Cuts at: 1517 4485 5792 1517
Size: 2968 1307 4270
Fragments arranged by size:
4270 2968 1307
StyI C'CwwG_G
Cuts at: 513 1065 2022 3372 3649 3758 3860 3924 5029
Size: 552 957 1350 277 109 102 64 1105
Cuts at: 5029 5196 5314 5984 513
Size: 167 118 670 3074
Fragments arranged by size:
3074 1350 1105 957 670 552 277 167
118 109 102 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2834 3537 3921 4719 5620 6572 6589
Size: 1255 1254 703 384 798 901 952 17
Cuts at: 6589 6742 6927 7372 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1254 952 901 798 703 445
384 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5208 6791 8503
Size: 274 80 33 51 1443 3231 1583 1712
Cuts at: 8503 96
Size: 138
Fragments arranged by size:
3231 1712 1583 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2930 2962 5107 5139 8505 8537 2930
Size: 32 2145 32 3366 32 2938
Fragments arranged by size:
3366 2938 2145 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3017 4130 4292 1316
Size: 1701 1113 162 5569
Fragments arranged by size:
5569 1701 1113 162
XbaI T'CTAG_A
Cuts at: 5881 5881
Size: 8545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2512 3922 2512
Size: 1410 7135
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8545
XmaI C'CCGG_G
Cuts at: 1870 5870 1870
Size: 4000 4545
XmnI GAAnn'nnTTC
Cuts at: 2313 6674 2313
Size: 4361 4184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4099 6353 276
Size: 53 83 186 936 2565 2254 2468
Fragments arranged by size:
2565 2468 2254 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsmBI BstZ17I EcoRV FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SexAI SrfI SwaI