Restriction map for
pFN21AB9399
(Circular) (Six-base) MAPSORT of: pFN21AB9399.seq Check: 7448 from: 1 to: 10078
pFN21AB9399 10078 bp
With 182 enzymes: SgfI *
April 12, 2013 16:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7888 278
Size: 53 83 186 936 6352 2468
Fragments arranged by size:
6352 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4699 4972 5595 7421 1937
Size: 2762 273 623 1826 4594
Fragments arranged by size:
4594 2762 1826 623 273
Acc65I G'GTAC_C
Cuts at: 7399 7399
Size: 10078
AclI AA'CG_TT
Cuts at: 1563 8205 8578 1563
Size: 6642 373 3063
Fragments arranged by size:
6642 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2296 2741 4003 4935 4985 6373 6858 8141
Size: 664 445 1262 932 50 1388 485 1283
Cuts at: 8141 9525 9793 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1388 1384 1283 1262 932 664 485
445 268 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9881
AflIII A'CryG_T
Cuts at: 1203 4905 6795 8977 9954 1203
Size: 3702 1890 2182 977 1327
Fragments arranged by size:
3702 2182 1890 1327 977
AgeI A'CCGG_T
Cuts at: 8909 8909
Size: 10078
AhdI GACnn_n'nnGTC
Cuts at: 8807 8807
Size: 10078
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3892 1184
Size: 2708 7370
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6545 6577 7118 7150 6545
Size: 32 541 32 9473
Fragments arranged by size:
9473 541 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2453 2967 3109 3283 3455 3694 5367 6581 6836
Size: 514 142 174 172 239 1673 1214 255
Cuts at: 6836 7821 9393 2453
Size: 985 1572 3138
Fragments arranged by size:
3138 1673 1572 1214 985 514 255 239
174 172 142
ApaLI G'TGCA_C
Cuts at: 8135 9291 8135
Size: 1156 8922
ApoI r'AATT_y
Cuts at: 1493 2047 2920 2930 3025 3841 4784 5612 6292
Size: 554 873 10 95 816 943 828 680
Cuts at: 6292 7387 7613 7765 9874 1493
Size: 1095 226 152 2109 1697
Fragments arranged by size:
2109 1697 1095 943 873 828 816 680
554 226 152 95 10
AseI AT'TA_AT
Cuts at: 160 1032 3003 5628 8632 160
Size: 872 1971 2625 3004 1606
Fragments arranged by size:
3004 2625 1971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10078
AvaI C'yCGr_G
Cuts at: 1870 1943 2857 7403 1870
Size: 73 914 4546 4545
Fragments arranged by size:
4546 4545 914 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4695 4728 4695
Size: 33 10045
BamHI G'GATC_C
Cuts at: 4942 6023 7408 7775 4942
Size: 1081 1385 367 7245
Fragments arranged by size:
7245 1385 1081 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2414 4753 5522 7399 8854
Size: 325 217 627 627 2339 769 1877 1455
Cuts at: 8854 618
Size: 1842
Fragments arranged by size:
2339 1877 1842 1455 769 627 627 325
217
BanII G_rGCy'C
Cuts at: 1391 2223 3126 3167 4105 4676 4851 7397 1391
Size: 832 903 41 938 571 175 2546 4072
Fragments arranged by size:
4072 2546 938 903 832 571 175 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2247 2491 3127 3772 4027 4366 6500
Size: 971 348 244 636 645 255 339 2134
Cuts at: 6500 928
Size: 4506
Fragments arranged by size:
4506 2134 971 645 636 348 339 255
244
BbvCI CC'TCA_GC
Cuts at: 2160 2795 2160
Size: 635 9443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 5833 5867 8269 8303 1903
Size: 34 2141 34 1721 34 2402 34 3678
Fragments arranged by size:
3678 2402 2141 1721 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7970 9180 7970
Size: 1210 8868
BclI T'GATC_A
Cuts at: 1548 4383 1548
Size: 2835 7243
BfrBI ATG'CAT
Cuts at: 2853 4349 5625 2853
Size: 1496 1276 7306
Fragments arranged by size:
7306 1496 1276
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8689 136
Size: 107 122 71 631 605 7017 1525
Fragments arranged by size:
7017 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6102 10073 6102
Size: 3971 6107
BlpI GC'TnA_GC
Cuts at: 5207 6674 7495 5207
Size: 1467 821 7790
Fragments arranged by size:
7790 1467 821
Bme1580I G_kGCm'C
Cuts at: 1790 5221 8139 9295 1790
Size: 3431 2918 1156 2573
Fragments arranged by size:
3431 2918 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2717 1318
Size: 1399 8679
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2018 2514 3129
Size: 416 53 83 399 24 601 496 615
Cuts at: 3129 3682 5476 6220 7809 8767 442
Size: 553 1794 744 1589 958 1753
Fragments arranged by size:
1794 1753 1589 958 744 615 601 553
496 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10078
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 4566 5130 3569
Size: 997 564 8517
Fragments arranged by size:
8517 997 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2164 5333 7298 8738 1239
Size: 925 3169 1965 1440 2579
Fragments arranged by size:
3169 2579 1965 1440 925
Bpu10I CC'TnA_GC
Cuts at: 2160 2795 4028 4824 6966 2160
Size: 635 1233 796 2142 5272
Fragments arranged by size:
5272 2142 1233 796 635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4574 5264 7139 8203 9068 9366 4574
Size: 690 1875 1064 865 298 5286
Fragments arranged by size:
5286 1875 1064 865 690 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4673 6080 8741 882
Size: 617 3174 1407 2661 2219
Fragments arranged by size:
3174 2661 2219 1407 617
BsaAI yAC'GTr
Cuts at: 493 1204 7869 493
Size: 711 6665 2702
Fragments arranged by size:
6665 2702 711
BsaBI GATnn'nnATC
Cuts at: 2099 10072 2099
Size: 7973 2105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4728 7885 8267 8902
Size: 53 83 186 936 3195 3157 382 635
Cuts at: 8902 275
Size: 1451
Fragments arranged by size:
3195 3157 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5442 8511 8909 9183 9330 9741 5442
Size: 3069 398 274 147 411 5779
Fragments arranged by size:
5779 3069 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 9853
Fragments arranged by size:
9853 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3230 4703 4781 6452 1186
Size: 2044 1473 78 1671 4812
Fragments arranged by size:
4812 2044 1671 1473 78
BseYI C'CCAG_C
Cuts at: 2148 3276 9281 9777 2148
Size: 1128 6005 496 2449
Fragments arranged by size:
6005 2449 1128 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4597 4611 6426 1163
Size: 573 2861 14 1815 4815
Fragments arranged by size:
4815 2861 1815 573 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7528 8289 9317 1540
Size: 275 186 5527 761 1028 2301
Fragments arranged by size:
5527 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 3571 4029 4413 4851 5458 6366 8139 8224
Size: 1348 458 384 438 607 908 1773 85
Cuts at: 8224 9295 2223
Size: 1071 3006
Fragments arranged by size:
3006 1773 1348 1071 908 607 458 438
384 85
BsmI GAATG_Cn'
Cuts at: 4351 7589 7682 4351
Size: 3238 93 6747
Fragments arranged by size:
6747 3238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 3126 3167 3571 4029 4105 4413
Size: 399 433 903 41 404 458 76 308
Cuts at: 4413 4676 4851 5221 5458 6366 7397 8139 8224
Size: 263 175 370 237 908 1031 742 85
Cuts at: 8224 9295 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1031 908 903 742 458 433
404 399 370 308 263 237 175 85
76 41
BspEI T'CCGG_A
Cuts at: 5442 9741 5442
Size: 4299 5779
BspHI T'CATG_A
Cuts at: 7967 9697 7967
Size: 1730 8348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5145 5156 5539 6647 7433 844
Size: 4301 11 383 1108 786 3489
Fragments arranged by size:
4301 3489 1108 786 383 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 7965 1394
Size: 429 221 5921 3507
Fragments arranged by size:
5921 3507 429 221
BsrDI GCAATG_nn'
Cuts at: 65 1248 2484 2899 3888 4422 4456 6015 6801
Size: 1183 1236 415 989 534 34 1559 786
Cuts at: 6801 7125 7333 8573 8747 65
Size: 324 208 1240 174 1396
Fragments arranged by size:
1559 1396 1240 1236 1183 989 786 534
415 324 208 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 8722 8909 1700
Size: 110 6912 187 2869
Fragments arranged by size:
6912 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 6798 96
Size: 6702 3376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10078
BssSI C'ACGA_G
Cuts at: 2214 3668 4873 6077 6444 8138 9150 2214
Size: 1454 1205 1204 367 1694 1012 3142
Fragments arranged by size:
3142 1694 1454 1205 1204 1012 367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3575 4299 4760 794
Size: 2781 724 461 6112
Fragments arranged by size:
6112 2781 724 461
BstBI TT'CG_AA
Cuts at: 7237 8882 7237
Size: 1645 8433
BstEII G'GTnAC_C
Cuts at: 1184 5019 7255 7265 7274 1184
Size: 3835 2236 10 9 3988
Fragments arranged by size:
3988 3835 2236 10 9
BstXI CCAn_nnnn'nTGG
Cuts at: 2592 3443 6734 2592
Size: 851 3291 5936
Fragments arranged by size:
5936 3291 851
BstYI r'GATC_y
Cuts at: 1972 2581 3547 4942 6023 6102 7408 7775 8160
Size: 609 966 1395 1081 79 1306 367 385
Cuts at: 8160 8177 9629 9715 9727 10073 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1395 1306 1081 966 609 385
367 346 86 79 17 12
BstZ17I GTA'TAC
Cuts at: 4700 4973 5596 4700
Size: 273 623 9182
Fragments arranged by size:
9182 623 273
Bsu36I CC'TnA_GG
Cuts at: 6425 6425
Size: 10078
BtgI C'CryG_G
Cuts at: 513 1065 2004 2585 3204 3315 3789 6050 7023
Size: 552 939 581 619 111 474 2261 973
Cuts at: 7023 513
Size: 3568
Fragments arranged by size:
3568 2261 973 939 619 581 552 474
111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9339
Fragments arranged by size:
9339 683 56
BtsI GCAGTG_nn'
Cuts at: 5218 7045 7594 8388 8408 5218
Size: 1827 549 794 20 6888
Fragments arranged by size:
6888 1827 794 549 20
ClaI AT'CG_AT
Cuts at: 1554 4516 6588 1554
Size: 2962 2072 5044
Fragments arranged by size:
5044 2962 2072
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2923 2958 529
Size: 35 2359 35 7649
Fragments arranged by size:
7649 2359 35 35
DraI TTT'AAA
Cuts at: 7382 7382
Size: 10078
DraIII CAC_nnn'GTG
Cuts at: 3575 3575
Size: 10078
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2606 6308 9085 809
Size: 507 1290 3702 2777 1802
Fragments arranged by size:
3702 2777 1802 1290 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3747 3786 4001
Size: 54 1425 50 275 18 1917 39 215
Cuts at: 4001 4055 4174 5962 6415 7353 7525 8414 10043
Size: 54 119 1788 453 938 172 889 1629
Cuts at: 10043 8
Size: 43
Fragments arranged by size:
1917 1788 1629 1425 938 889 453 275
215 172 119 54 54 50 43 39
18
EagI C'GGCC_G
Cuts at: 1537 1812 7525 1537
Size: 275 5713 4090
Fragments arranged by size:
5713 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 3957 4424 5472 5915 6392 6641 6715
Size: 1478 1126 467 1048 443 477 249 74
Cuts at: 6715 7122 7194 8008 1353
Size: 407 72 814 3423
Fragments arranged by size:
3423 1478 1126 1048 814 477 467 443
407 249 74 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 4511 8665 9039 9185 387
Size: 1064 138 453 2469 4154 374 146 1280
Fragments arranged by size:
4154 2469 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 2221 4849 2221
Size: 2628 7450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2164 2296 2741 4003 4935 4985 5333
Size: 393 532 132 445 1262 932 50 348
Cuts at: 5333 6373 6858 7298 8141 8738 9525 9793 1239
Size: 1040 485 440 843 597 787 268 1524
Fragments arranged by size:
1524 1262 1040 932 843 787 597 532
485 445 440 393 348 268 132 50
EcoO109I rG'GnC_Cy
Cuts at: 2450 2736 3709 6850 2450
Size: 286 973 3141 5678
Fragments arranged by size:
5678 3141 973 286
EcoRI G'AATT_C
Cuts at: 2930 7387 7765 2930
Size: 4457 378 5243
Fragments arranged by size:
5243 4457 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 3427 4606 2344
Size: 1083 1179 7816
Fragments arranged by size:
7816 1179 1083
FspI TGC'GCA
Cuts at: 8584 8584
Size: 10078
HaeII r_GCGC'y
Cuts at: 1678 9225 1678
Size: 7547 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2367 2399 4895 4927 7021
Size: 32 135 32 795 32 2496 32 2094
Cuts at: 7021 7053 8719 8751 8793 8825 1373
Size: 32 1666 32 42 32 2626
Fragments arranged by size:
2626 2496 2094 1666 795 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2767 5719 6793 7422 7668 669
Size: 988 281 829 2952 1074 629 246 3079
Fragments arranged by size:
3079 2952 1074 988 829 629 281 246
HindIII A'AGCT_T
Cuts at: 748 2523 2889 3092 748
Size: 1775 366 203 7734
Fragments arranged by size:
7734 1775 366 203
HpaI GTT'AAC
Cuts at: 7668 7668
Size: 10078
KpnI G_GTAC'C
Cuts at: 7403 7403
Size: 10078
MfeI C'AATT_G
Cuts at: 2082 7677 2082
Size: 5595 4483
MluI A'CGCG_T
Cuts at: 9954 9954
Size: 10078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 4078 6404 9192 9376 1305
Size: 786 1987 2326 2788 184 2007
Fragments arranged by size:
2788 2326 2007 1987 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3749 3788 4003 4057 5964
Size: 54 1425 343 1917 39 215 54 1907
Cuts at: 5964 7355 10
Size: 1391 2733
Fragments arranged by size:
2733 1917 1907 1425 1391 343 215 54
54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2951 3534 3599 4360 4661 5390 5975
Size: 648 1785 583 65 761 301 729 585
Cuts at: 5975 6806 8036 8395 8554 518
Size: 831 1230 359 159 2042
Fragments arranged by size:
2042 1785 1230 831 761 729 648 585
583 359 301 159 65
MspA1I CmG'CkG
Cuts at: 1606 3283 5172 5801 7494 8171 9319 9564 1606
Size: 1677 1889 629 1693 677 1148 245 2120
Fragments arranged by size:
2120 1889 1693 1677 1148 677 629 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2585 3204 3315 3789 7023 513
Size: 552 939 581 619 111 474 3234 3568
Fragments arranged by size:
3568 3234 939 619 581 552 474 111
NdeI CA'TA_TG
Cuts at: 387 3211 387
Size: 2824 7254
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10078
NotI GC'GGCC_GC
Cuts at: 7525 7525
Size: 10078
NsiI A_TGCA'T
Cuts at: 2855 4351 5627 2855
Size: 1496 1276 7306
Fragments arranged by size:
7306 1496 1276
NspI r_CATG'y
Cuts at: 1132 1229 2327 2404 4165 4909 6628 6799 7436
Size: 97 1098 77 1761 744 1719 171 637
Cuts at: 7436 8981 1132
Size: 1545 2229
Fragments arranged by size:
2229 1761 1719 1545 1098 744 637 171
97 77
PasI CC'CwG_GG
Cuts at: 1348 4723 5118 1348
Size: 3375 395 6308
Fragments arranged by size:
6308 3375 395
PciI A'CATG_T
Cuts at: 4905 6795 8977 4905
Size: 1890 2182 6006
Fragments arranged by size:
6006 2182 1890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4053 5367 6288 6581 7051 1108
Size: 2945 1314 921 293 470 4135
Fragments arranged by size:
4135 2945 1314 921 470 293
PfoI T'CCnGG_A
Cuts at: 2180 2638 5310 2180
Size: 458 2672 6948
Fragments arranged by size:
6948 2672 458
PmeI GTTT'AAAC
Cuts at: 7382 7382
Size: 10078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8147 8179 9660 9692 8147
Size: 32 1481 32 8533
Fragments arranged by size:
8533 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3709 6850 3709
Size: 3141 6937
PshAI GACnn'nnGTC
Cuts at: 1613 4121 1613
Size: 2508 7570
PsiI TTA'TAA
Cuts at: 5648 7648 5648
Size: 2000 8078
PstI C_TGCA'G
Cuts at: 830 3241 7430 830
Size: 2411 4189 3478
Fragments arranged by size:
4189 3478 2411
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10078
PvuII CAG'CTG
Cuts at: 3283 5172 5801 3283
Size: 1889 629 7560
Fragments arranged by size:
7560 1889 629
SacI G_AGCT'C
Cuts at: 2223 4851 2223
Size: 2628 7450
SalI G'TCGA_C
Cuts at: 1936 7420 1936
Size: 5484 4594
SapI GCTCTTCn'nnn_
Cuts at: 2831 3957 6392 2831
Size: 1126 2435 6517
Fragments arranged by size:
6517 2435 1126
SbfI CC_TGCA'GG
Cuts at: 7430 7430
Size: 10078
ScaI AGT'ACT
Cuts at: 8326 10038 8326
Size: 1712 8366
SfcI C'TryA_G
Cuts at: 826 1045 2445 3237 5092 7426 8561 9242 9433
Size: 219 1400 792 1855 2334 1135 681 191
Cuts at: 9433 826
Size: 1471
Fragments arranged by size:
2334 1855 1471 1400 1135 792 681 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10078
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10078
SmaI CCC'GGG
Cuts at: 1872 7405 1872
Size: 5533 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2857 4553 5243 7154 8182 9083
Size: 197 926 914 1696 690 1911 1028 901
Cuts at: 9083 9345 820
Size: 262 1553
Fragments arranged by size:
1911 1696 1553 1028 926 914 901 690
262 197
SnaBI TAC'GTA
Cuts at: 493 7869 493
Size: 7376 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10078
SphI G_CATG'C
Cuts at: 1132 2327 4165 6628 7436 1132
Size: 1195 1838 2463 808 3774
Fragments arranged by size:
3774 2463 1838 1195 808
SspI AAT'ATT
Cuts at: 5 52 2538 6932 8002 5
Size: 47 2486 4394 1070 2081
Fragments arranged by size:
4394 2486 2081 1070 47
StuI AGG'CCT
Cuts at: 1517 2177 4841 5464 7232 1517
Size: 660 2664 623 1768 4363
Fragments arranged by size:
4363 2664 1768 660 623
StyI C'CwwG_G
Cuts at: 513 1065 2004 2585 3204 3252 3315 3789 4105
Size: 552 939 581 619 48 63 474 316
Cuts at: 4105 6348 7023 7260 7270 7356 7517 513
Size: 2243 675 237 10 86 161 3074
Fragments arranged by size:
3074 2243 939 675 619 581 552 474
316 237 161 86 63 48 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6848 8105 8122 8275 8460 8905 325
Size: 1255 5268 1257 17 153 185 445 1498
Fragments arranged by size:
5268 1498 1257 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3982 5279 6332
Size: 274 80 33 51 1443 2005 1297 1053
Cuts at: 6332 6798 7242 8324 10036 96
Size: 466 444 1082 1712 138
Fragments arranged by size:
2005 1712 1443 1297 1082 1053 466 444
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4389 4421 5484 5516 10038 10070 4389
Size: 32 1063 32 4522 32 4397
Fragments arranged by size:
4522 4397 1063 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6089 1316
Size: 4773 5305
XbaI T'CTAG_A
Cuts at: 7414 7414
Size: 10078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4691 4691
Size: 10078
XhoI C'TCGA_G
Cuts at: 1943 2857 1943
Size: 914 9164
XmaI C'CCGG_G
Cuts at: 1870 7403 1870
Size: 5533 4545
XmnI GAAnn'nnTTC
Cuts at: 3407 5235 5438 5909 8207 3407
Size: 1828 203 471 2298 5278
Fragments arranged by size:
5278 2298 1828 471 203
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7886 276
Size: 53 83 186 936 6352 2468
Fragments arranged by size:
6352 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BsiWI BsmBI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI