Restriction map for
pFN21AB9397
(Circular) (Six-base) MAPSORT of: pFN21AB9397.seq Check: 7232 from: 1 to: 6655
pFN21AB9397 6655 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2824 2824
Size: 6655
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4465 278
Size: 53 83 186 936 2929 2468
Fragments arranged by size:
2929 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3998 1937
Size: 2061 4594
Acc65I G'GTAC_C
Cuts at: 3976 3976
Size: 6655
AclI AA'CG_TT
Cuts at: 1563 4782 5155 1563
Size: 3219 373 3063
Fragments arranged by size:
3219 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 2232 2296 2387 4718 6102 6370 1632
Size: 576 24 64 91 2331 1384 268 1917
Fragments arranged by size:
2331 1917 1384 576 268 91 64 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6458
AflIII A'CryG_T
Cuts at: 1203 3168 5554 6531 1203
Size: 1965 2386 977 1327
Fragments arranged by size:
2386 1965 1327 977
AgeI A'CCGG_T
Cuts at: 5486 5486
Size: 6655
AhdI GACnn_n'nnGTC
Cuts at: 5384 5384
Size: 6655
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6655
AlwNI CAG_nnn'CTG
Cuts at: 2195 4398 5970 2195
Size: 2203 1572 2880
Fragments arranged by size:
2880 2203 1572
ApaLI G'TGCA_C
Cuts at: 4712 5868 4712
Size: 1156 5499
ApoI r'AATT_y
Cuts at: 1493 2906 3223 3964 4190 4342 6451 1493
Size: 1413 317 741 226 152 2109 1697
Fragments arranged by size:
2109 1697 1413 741 317 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5209 160
Size: 872 4177 1606
Fragments arranged by size:
4177 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6655
AvaI C'yCGr_G
Cuts at: 1870 1943 2826 3111 3980 1870
Size: 73 883 285 869 4545
Fragments arranged by size:
4545 883 869 285 73
AvrII C'CTAG_G
Cuts at: 3863 3863
Size: 6655
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2399 2432 2399
Size: 33 6622
BamHI G'GATC_C
Cuts at: 3735 3985 4352 3735
Size: 250 367 6038
Fragments arranged by size:
6038 367 250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 3976 5431 618
Size: 325 217 627 768 1421 1455 1842
Fragments arranged by size:
1842 1455 1421 768 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2772 3974 1391
Size: 1381 1202 4072
Fragments arranged by size:
4072 1381 1202
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2361 2447 2463 928
Size: 971 462 86 16 5120
Fragments arranged by size:
5120 971 462 86 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4846 4880 1903
Size: 34 2909 34 3678
Fragments arranged by size:
3678 2909 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2702 4547 5757 2702
Size: 1845 1210 3600
Fragments arranged by size:
3600 1845 1210
BclI T'GATC_A
Cuts at: 1548 2592 1548
Size: 1044 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2275 5266 136
Size: 107 122 71 631 605 603 2991 1525
Fragments arranged by size:
2991 1525 631 605 603 122 107 71
BglII A'GATC_T
Cuts at: 2807 3459 6650 2807
Size: 652 3191 2812
Fragments arranged by size:
3191 2812 652
BlpI GC'TnA_GC
Cuts at: 3714 4072 3714
Size: 358 6297
Bme1580I G_kGCm'C
Cuts at: 1790 2560 4716 5872 1790
Size: 770 2156 1156 2573
Fragments arranged by size:
2573 2156 1156 770
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6655
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3741 4386 5344
Size: 416 53 83 399 24 2324 645 958
Cuts at: 5344 442
Size: 1753
Fragments arranged by size:
2324 1753 958 645 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2979 3603 2979
Size: 624 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3776 5315 1239
Size: 2537 1539 2579
Fragments arranged by size:
2579 2537 1539
Bpu10I CC'TnA_GC
Cuts at: 3693 3693
Size: 6655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2974 3581 3909 4780 5645 5943 2974
Size: 607 328 871 865 298 3686
Fragments arranged by size:
3686 871 865 607 328 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 5318 882
Size: 617 675 3144 2219
Fragments arranged by size:
3144 2219 675 617
BsaAI yAC'GTr
Cuts at: 493 1204 4446 493
Size: 711 3242 2702
Fragments arranged by size:
3242 2702 711
BsaBI GATnn'nnATC
Cuts at: 3048 6649 3048
Size: 3601 3054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2942 4462 4844
Size: 53 83 186 936 476 933 1520 382
Cuts at: 4844 5479 275
Size: 635 1451
Fragments arranged by size:
1520 1451 936 933 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3738 5088 5486 5760 5907 6318 3738
Size: 1350 398 274 147 411 4075
Fragments arranged by size:
4075 1350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3899 3929 1178
Size: 30 165 30 2496 30 3904
Fragments arranged by size:
3904 2496 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6655
BseYI C'CCAG_C
Cuts at: 5858 6354 5858
Size: 496 6159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2802 1163
Size: 573 1066 5016
Fragments arranged by size:
5016 1066 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4105 4866 5894 1540
Size: 275 186 2104 761 1028 2301
Fragments arranged by size:
2301 2104 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3857 4716 4801 5872 3857
Size: 859 85 1071 4640
Fragments arranged by size:
4640 1071 859 85
BsmI GAATG_Cn'
Cuts at: 2154 4166 4259 2154
Size: 2012 93 4550
Fragments arranged by size:
4550 2012 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 6655
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2560 2772 3857 3974 4716 4801 5872
Size: 399 770 212 1085 117 742 85 1071
Cuts at: 5872 1391
Size: 2174
Fragments arranged by size:
2174 1085 1071 770 742 399 212 117
85
BspEI T'CCGG_A
Cuts at: 3738 6318 3738
Size: 2580 4075
BspHI T'CATG_A
Cuts at: 4544 6274 4544
Size: 1730 4925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2203 2824 4010 844
Size: 1359 621 1186 3489
Fragments arranged by size:
3489 1359 1186 621
BsrBI CCG'CTC
Cuts at: 1394 1823 3766 4542 1394
Size: 429 1943 776 3507
Fragments arranged by size:
3507 1943 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2087 3459 5150 5324 65
Size: 1183 839 1372 1691 174 1396
Fragments arranged by size:
1691 1396 1372 1183 839 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5299 5486 1700
Size: 110 3489 187 2869
Fragments arranged by size:
3489 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6655
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6655
BssSI C'ACGA_G
Cuts at: 3590 4715 5727 3590
Size: 1125 1012 4518
Fragments arranged by size:
4518 1125 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3685 794
Size: 2891 3764
BstBI TT'CG_AA
Cuts at: 5459 5459
Size: 6655
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6655
BstYI r'GATC_y
Cuts at: 1972 2807 3244 3459 3735 3985 4352 4737 4754
Size: 835 437 215 276 250 367 385 17
Cuts at: 4754 6206 6292 6304 6650 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 835 437 385 367 346 276
250 215 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3830 513
Size: 552 939 1826 3338
Fragments arranged by size:
3338 1826 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2892 487
Size: 683 56 1666 4250
Fragments arranged by size:
4250 1666 683 56
BtsI GCAGTG_nn'
Cuts at: 3310 4171 4965 4985 3310
Size: 861 794 20 4980
Fragments arranged by size:
4980 861 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6655
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6620
DraI TTT'AAA
Cuts at: 3959 3959
Size: 6655
DraIII CAC_nnn'GTG
Cuts at: 3285 3285
Size: 6655
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2465 5662 809
Size: 507 1149 3197 1802
Fragments arranged by size:
3197 1802 1149 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2723 3777 4102
Size: 54 1425 50 275 18 893 1054 325
Cuts at: 4102 4991 6620 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1054 893 889 325 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4102 1537
Size: 275 2290 4090
Fragments arranged by size:
4090 2290 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2245 3521 3639 4585 1353
Size: 892 1276 118 946 3423
Fragments arranged by size:
3423 1276 946 892 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 5242 5616 5762 387
Size: 1064 138 465 3188 374 146 1280
Fragments arranged by size:
3188 1280 1064 465 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2232 2296 2387 3776 4718 5315
Size: 393 576 24 64 91 1389 942 597
Cuts at: 5315 6102 6370 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1389 942 787 597 576 393 268
91 64 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3862 3862
Size: 6655
EcoO109I rG'GnC_Cy
Cuts at: 2192 2192
Size: 6655
EcoRI G'AATT_C
Cuts at: 3964 4342 3964
Size: 378 6277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2826 3950 2826
Size: 1124 5531
FspI TGC'GCA
Cuts at: 5161 5161
Size: 6655
HaeII r_GCGC'y
Cuts at: 1678 5802 1678
Size: 4124 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5296 5328 5370 5402 1373
Size: 32 135 32 3724 32 42 32 2626
Fragments arranged by size:
3724 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3999 4245 669
Size: 988 281 1224 837 246 3079
Fragments arranged by size:
3079 1224 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 3122 748
Size: 2374 4281
HpaI GTT'AAC
Cuts at: 3162 4245 3162
Size: 1083 5572
KpnI G_GTAC'C
Cuts at: 3980 3980
Size: 6655
MfeI C'AATT_G
Cuts at: 4254 4254
Size: 6655
MluI A'CGCG_T
Cuts at: 6531 6531
Size: 6655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 2350 2817 3285 5769 5953 1305
Size: 732 313 467 468 2484 184 2007
Fragments arranged by size:
2484 2007 732 468 467 313 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2725 3779 10
Size: 54 1425 343 893 1054 2886
Fragments arranged by size:
2886 1425 1054 893 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2553 3147 4613 4972 5131 518
Size: 648 1387 594 1466 359 159 2042
Fragments arranged by size:
2042 1466 1387 648 594 359 159
MspA1I CmG'CkG
Cuts at: 1606 2617 4071 4748 5896 6141 1606
Size: 1011 1454 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 1011 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3830 513
Size: 552 939 1826 3338
Fragments arranged by size:
3338 1826 939 552
NdeI CA'TA_TG
Cuts at: 387 2862 387
Size: 2475 4180
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6655
NotI GC'GGCC_GC
Cuts at: 4102 4102
Size: 6655
NspI r_CATG'y
Cuts at: 1132 1229 2055 4013 5558 1132
Size: 97 826 1958 1545 2229
Fragments arranged by size:
2229 1958 1545 826 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6655
PciI A'CATG_T
Cuts at: 5554 5554
Size: 6655
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6655
PmeI GTTT'AAAC
Cuts at: 3959 3959
Size: 6655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4724 4756 6237 6269 4724
Size: 32 1481 32 5110
Fragments arranged by size:
5110 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2192 2192
Size: 6655
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6655
PsiI TTA'TAA
Cuts at: 3230 4225 3230
Size: 995 5660
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2391 2423 2391
Size: 32 6623
PstI C_TGCA'G
Cuts at: 830 2565 4007 830
Size: 1735 1442 3478
Fragments arranged by size:
3478 1735 1442
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6655
SalI G'TCGA_C
Cuts at: 1936 3997 1936
Size: 2061 4594
SbfI CC_TGCA'GG
Cuts at: 2565 4007 2565
Size: 1442 5213
ScaI AGT'ACT
Cuts at: 2650 4903 6615 2650
Size: 2253 1712 2690
Fragments arranged by size:
2690 2253 1712
SfcI C'TryA_G
Cuts at: 826 1045 2561 2736 2901 4003 5138 5819 6010
Size: 219 1516 175 165 1102 1135 681 191
Cuts at: 6010 826
Size: 1471
Fragments arranged by size:
1516 1471 1135 1102 681 219 191 175
165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6655
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6655
SmaI CCC'GGG
Cuts at: 1872 2828 3982 1872
Size: 956 1154 4545
Fragments arranged by size:
4545 1154 956
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2953 3560 3924 4759 5660 5922
Size: 197 926 1010 607 364 835 901 262
Cuts at: 5922 820
Size: 1553
Fragments arranged by size:
1553 1010 926 901 835 607 364 262
197
SnaBI TAC'GTA
Cuts at: 493 4446 493
Size: 3953 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6655
SphI G_CATG'C
Cuts at: 1132 4013 1132
Size: 2881 3774
SspI AAT'ATT
Cuts at: 5 52 4579 5
Size: 47 4527 2081
Fragments arranged by size:
4527 2081 47
StuI AGG'CCT
Cuts at: 1517 3095 3545 1517
Size: 1578 450 4627
Fragments arranged by size:
4627 1578 450
StyI C'CwwG_G
Cuts at: 513 1065 2004 3830 3863 4094 513
Size: 552 939 1826 33 231 3074
Fragments arranged by size:
3074 1826 939 552 231 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 4682 4699 4852 5037 5482 325
Size: 1255 1035 2067 17 153 185 445 1498
Fragments arranged by size:
2067 1498 1255 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2054 2363 2622
Size: 274 80 33 51 1443 77 309 259
Cuts at: 2622 2648 3840 4901 6613 96
Size: 26 1192 1061 1712 138
Fragments arranged by size:
1712 1443 1192 1061 309 274 259 138
80 77 51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3733 3765 6615 6647 3733
Size: 32 2850 32 3741
Fragments arranged by size:
3741 2850 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6655
XbaI T'CTAG_A
Cuts at: 2389 3991 2389
Size: 1602 5053
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6655
XmaI C'CCGG_G
Cuts at: 1870 2826 3980 1870
Size: 956 1154 4545
Fragments arranged by size:
4545 1154 956
XmnI GAAnn'nnTTC
Cuts at: 2809 3612 4784 2809
Size: 803 1172 4680
Fragments arranged by size:
4680 1172 803
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4463 276
Size: 53 83 186 936 2929 2468
Fragments arranged by size:
2929 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI