Restriction map for
pFN21AB9394
(Circular) (Six-base) MAPSORT of: pFN21AB9394.seq Check: 2655 from: 1 to: 5869
pFN21AB9394 5869 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2194 2562 2194
Size: 368 5501
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3212 1937
Size: 1275 4594
Acc65I G'GTAC_C
Cuts at: 2615 3190 2615
Size: 575 5294
AclI AA'CG_TT
Cuts at: 1563 2649 3996 4369 1563
Size: 1086 1347 373 3063
Fragments arranged by size:
3063 1347 1086 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 3932 5316 5584 1632
Size: 702 1598 1384 268 1917
Fragments arranged by size:
1917 1598 1384 702 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 4768 5745 1203
Size: 3565 977 1327
Fragments arranged by size:
3565 1327 977
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5869
AlwNI CAG_nnn'CTG
Cuts at: 2581 3612 5184 2581
Size: 1031 1572 3266
Fragments arranged by size:
3266 1572 1031
ApaI G_GGCC'C
Cuts at: 2375 2375
Size: 5869
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 3178 3404 3556 5665 1493
Size: 1685 226 152 2109 1697
Fragments arranged by size:
2109 1697 1685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 3143 3194 1870
Size: 73 1200 51 4545
Fragments arranged by size:
4545 1200 73 51
BamHI G'GATC_C
Cuts at: 3199 3566 3199
Size: 367 5502
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 2615 3190 4645 618
Size: 325 217 627 320 508 575 1455 1842
Fragments arranged by size:
1842 1455 627 575 508 325 320 217
BanII G_rGCy'C
Cuts at: 1391 2375 3188 1391
Size: 984 813 4072
Fragments arranged by size:
4072 984 813
BbeI G_GCGC'C
Cuts at: 2111 2111
Size: 5869
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 928
Size: 971 423 4475
Fragments arranged by size:
4475 971 423
BbvCI CC'TCA_GC
Cuts at: 3035 3035
Size: 5869
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2192 2226 4060 4094 1903
Size: 34 50 34 171 34 1834 34 3678
Fragments arranged by size:
3678 1834 171 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 4971 3761
Size: 1210 4659
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4480 136
Size: 107 122 71 631 605 2808 1525
Fragments arranged by size:
2808 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5864 5864
Size: 5869
BlpI GC'TnA_GC
Cuts at: 3286 3286
Size: 5869
Bme1580I G_kGCm'C
Cuts at: 1790 2375 3930 5086 1790
Size: 585 1555 1156 2573
Fragments arranged by size:
2573 1555 1156 585
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2531 3600 4558
Size: 416 53 83 399 24 1114 1069 958
Cuts at: 4558 442
Size: 1753
Fragments arranged by size:
1753 1114 1069 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2552 4529 1239
Size: 1313 1977 2579
Fragments arranged by size:
2579 1977 1313
Bpu10I CC'TnA_GC
Cuts at: 2190 3035 3153 2190
Size: 845 118 4906
Fragments arranged by size:
4906 845 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3994 4859 5157 3994
Size: 865 298 4706
Fragments arranged by size:
4706 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4532 882
Size: 617 3033 2219
Fragments arranged by size:
3033 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3660 493
Size: 711 2456 2702
Fragments arranged by size:
2702 2456 711
BsaBI GATnn'nnATC
Cuts at: 2504 2709 5863 2504
Size: 205 3154 2510
Fragments arranged by size:
3154 2510 205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2108 3108 3676 4058
Size: 53 83 186 936 575 1000 568 382
Cuts at: 4058 4693 275
Size: 635 1451
Fragments arranged by size:
1451 1000 936 635 575 568 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2514 2719 4302 4700 4974 5121 5532 2514
Size: 205 1583 398 274 147 411 2851
Fragments arranged by size:
2851 1583 411 398 274 205 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5644
Fragments arranged by size:
5644 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3015 1186
Size: 1829 4040
BseYI C'CCAG_C
Cuts at: 5072 5568 5072
Size: 496 5373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5296
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3319 4080 5108 1540
Size: 275 186 1318 761 1028 2301
Fragments arranged by size:
2301 1318 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2191 2532 3930 4015 5086 2191
Size: 341 1398 85 1071 2974
Fragments arranged by size:
2974 1398 1071 341 85
BsmI GAATG_Cn'
Cuts at: 2154 2935 3380 3473 2154
Size: 781 445 93 4550
Fragments arranged by size:
4550 781 445 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2191 2375 2532 3188 3930 4015 5086
Size: 399 401 184 157 656 742 85 1071
Cuts at: 5086 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 656 401 399 184 157
85
BspEI T'CCGG_A
Cuts at: 2514 5532 2514
Size: 3018 2851
BspHI T'CATG_A
Cuts at: 2136 2416 2701 3758 5488 2136
Size: 280 285 1057 1730 2517
Fragments arranged by size:
2517 1730 1057 285 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2194 2351 2562 3224 844
Size: 1350 157 211 662 3489
Fragments arranged by size:
3489 1350 662 211 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2043 3756 1394
Size: 429 220 1713 3507
Fragments arranged by size:
3507 1713 429 220
BsrDI GCAATG_nn'
Cuts at: 65 1248 2673 4364 4538 65
Size: 1183 1425 1691 174 1396
Fragments arranged by size:
1691 1425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2949 4513 4700 1700
Size: 110 1139 1564 187 2869
Fragments arranged by size:
2869 1564 1139 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5869
BssHII G'CGCG_C
Cuts at: 1925 2008 1925
Size: 83 5786
BssSI C'ACGA_G
Cuts at: 2525 3929 4941 2525
Size: 1404 1012 3453
Fragments arranged by size:
3453 1404 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5869
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstXI CCAn_nnnn'nTGG
Cuts at: 3017 3017
Size: 5869
BstYI r'GATC_y
Cuts at: 1972 3199 3566 3951 3968 5420 5506 5518 5864
Size: 1227 367 385 17 1452 86 12 346
Cuts at: 5864 1972
Size: 1977
Fragments arranged by size:
1977 1452 1227 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5130
Fragments arranged by size:
5130 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3385 4179 4199 2245
Size: 1140 794 20 3915
Fragments arranged by size:
3915 1140 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 809
Size: 507 3560 1802
Fragments arranged by size:
3560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2951 3316 4205
Size: 54 1425 50 275 18 1121 365 889
Cuts at: 4205 5834 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1121 889 365 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3316 1537
Size: 275 1504 4090
Fragments arranged by size:
4090 1504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2138 2429 3799 1353
Size: 713 72 291 1370 3423
Fragments arranged by size:
3423 1370 713 291 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2838 4456 4830 4976 387
Size: 1064 138 1249 1618 374 146 1280
Fragments arranged by size:
1618 1280 1249 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2552 3932 4529 5316 5584 1239
Size: 393 702 218 1380 597 787 268 1524
Fragments arranged by size:
1524 1380 787 702 597 393 268 218
EcoO109I rG'GnC_Cy
Cuts at: 2371 2371
Size: 5869
EcoRI G'AATT_C
Cuts at: 3178 3556 3178
Size: 378 5491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2376 2376
Size: 5869
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 2111 2575 2581 5016 1678
Size: 433 464 6 2435 2531
Fragments arranged by size:
2531 2435 464 433 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2991 3023 4510 4542 4584
Size: 32 135 32 1419 32 1487 32 42
Cuts at: 4584 4616 1373
Size: 32 2626
Fragments arranged by size:
2626 1487 1419 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3133 3213 3459 669
Size: 988 281 1195 80 246 3079
Fragments arranged by size:
3079 1195 988 281 246 80
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KasI G'GCGC_C
Cuts at: 2107 2107
Size: 5869
KpnI G_GTAC'C
Cuts at: 2619 3194 2619
Size: 575 5294
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4983 5167 1305
Size: 3678 184 2007
Fragments arranged by size:
3678 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4047
Fragments arranged by size:
4047 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 3827 4186 4345 518
Size: 648 1221 1440 359 159 2042
Fragments arranged by size:
2042 1440 1221 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3285 3962 5110 5355 1606
Size: 1679 677 1148 245 2120
Fragments arranged by size:
2120 1679 1148 677 245
NaeI GCC'GGC
Cuts at: 2951 2951
Size: 5869
NarI GG'CG_CC
Cuts at: 2108 2108
Size: 5869
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5317
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NgoMIV G'CCGG_C
Cuts at: 2949 2949
Size: 5869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 2482 3227 4772 1132
Size: 97 1253 745 1545 2229
Fragments arranged by size:
2229 1545 1253 745 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5869
PciI A'CATG_T
Cuts at: 4768 4768
Size: 5869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5869
PfoI T'CCnGG_A
Cuts at: 2163 2163
Size: 5869
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PspOMI G'GGCC_C
Cuts at: 2371 2371
Size: 5869
PstI C_TGCA'G
Cuts at: 830 2586 3221 830
Size: 1756 635 3478
Fragments arranged by size:
3478 1756 635
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SapI GCTCTTCn'nnn_
Cuts at: 2066 2066
Size: 5869
SbfI CC_TGCA'GG
Cuts at: 3221 3221
Size: 5869
ScaI AGT'ACT
Cuts at: 4117 5829 4117
Size: 1712 4157
SfcI C'TryA_G
Cuts at: 826 1045 2582 3217 4352 5033 5224 826
Size: 219 1537 635 1135 681 191 1471
Fragments arranged by size:
1537 1471 1135 681 635 219 191
SfoI GGC'GCC
Cuts at: 2109 2109
Size: 5869
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 3196 1872
Size: 1324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3973 4874 5136 820
Size: 197 926 2030 901 262 1553
Fragments arranged by size:
2030 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 2482 3227 1132
Size: 1350 745 3774
Fragments arranged by size:
3774 1350 745
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5869
StyI C'CwwG_G
Cuts at: 513 1065 2149 3308 513
Size: 552 1084 1159 3074
Fragments arranged by size:
3074 1159 1084 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3896 3913 4066 4251 4696 325
Size: 1255 2316 17 153 185 445 1498
Fragments arranged by size:
2316 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 2923 2973
Size: 274 80 33 51 1443 114 832 50
Cuts at: 2973 4115 5827 96
Size: 1142 1712 138
Fragments arranged by size:
1712 1443 1142 832 274 138 114 80
51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 5829 5861 2224
Size: 32 3573 32 2232
Fragments arranged by size:
3573 2232 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5869
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 3194 1870
Size: 1324 4545
XmnI GAAnn'nnTTC
Cuts at: 2850 3998 2850
Size: 1148 4721
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI XcmI