Restriction map for
pFN21AB9392
(Circular) (Six-base) MAPSORT of: pFN21AB9392.seq Check: 9480 from: 1 to: 7111
pFN21AB9392 7111 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3398 3398
Size: 7111
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4921 278
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3501 3901 4454 1937
Size: 1564 400 553 4594
Fragments arranged by size:
4594 1564 553 400
Acc65I G'GTAC_C
Cuts at: 4432 4432
Size: 7111
AclI AA'CG_TT
Cuts at: 1563 5238 5611 1563
Size: 3675 373 3063
Fragments arranged by size:
3675 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2519 2895 3561 3759 3891 3915 5174 6558
Size: 887 376 666 198 132 24 1259 1384
Cuts at: 6558 6826 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1259 887 666 376 268 198
132 24
AfeI AGC'GCT
Cuts at: 4280 4280
Size: 7111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6914
AflIII A'CryG_T
Cuts at: 1203 2058 6010 6987 1203
Size: 855 3952 977 1327
Fragments arranged by size:
3952 1327 977 855
AgeI A'CCGG_T
Cuts at: 5942 5942
Size: 7111
AhdI GACnn_n'nnGTC
Cuts at: 5840 5840
Size: 7111
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7111
AlwNI CAG_nnn'CTG
Cuts at: 2234 3085 4129 4854 6426 2234
Size: 851 1044 725 1572 2919
Fragments arranged by size:
2919 1572 1044 851 725
ApaLI G'TGCA_C
Cuts at: 2044 3570 5168 6324 2044
Size: 1526 1598 1156 2831
Fragments arranged by size:
2831 1598 1526 1156
ApoI r'AATT_y
Cuts at: 1493 2856 3435 4168 4420 4646 4798 6907 1493
Size: 1363 579 733 252 226 152 2109 1697
Fragments arranged by size:
2109 1697 1363 733 579 252 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5665 160
Size: 872 4633 1606
Fragments arranged by size:
4633 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
AvaI C'yCGr_G
Cuts at: 1870 1943 4436 1870
Size: 73 2493 4545
Fragments arranged by size:
4545 2493 73
BamHI G'GATC_C
Cuts at: 4441 4808 4441
Size: 367 6744
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4432 5887 618
Size: 325 217 627 2645 1455 1842
Fragments arranged by size:
2645 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2944 4430 1391
Size: 1553 1486 4072
Fragments arranged by size:
4072 1553 1486
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2625 2825 928
Size: 971 726 200 5214
Fragments arranged by size:
5214 971 726 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5302 5336 1903
Size: 34 3365 34 3678
Fragments arranged by size:
3678 3365 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2004 3924 5003 6213 2004
Size: 1920 1079 1210 2902
Fragments arranged by size:
2902 1920 1210 1079
BclI T'GATC_A
Cuts at: 1548 2236 3583 1548
Size: 688 1347 5076
Fragments arranged by size:
5076 1347 688
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5722 136
Size: 107 122 71 631 605 4050 1525
Fragments arranged by size:
4050 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7106 7106
Size: 7111
BlpI GC'TnA_GC
Cuts at: 4528 4528
Size: 7111
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2356 3574 5172 6328 1790
Size: 258 308 1218 1598 1156 2573
Fragments arranged by size:
2573 1598 1218 1156 308 258
BmgBI CAC'GTC
Cuts at: 1318 2043 2059 2116 1318
Size: 725 16 57 6313
Fragments arranged by size:
6313 725 57 16
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3289 3972 4842
Size: 416 53 83 399 24 1872 683 870
Cuts at: 4842 5800 442
Size: 958 1753
Fragments arranged by size:
1872 1753 958 870 683 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2958 5771 1239
Size: 1719 2813 2579
Fragments arranged by size:
2813 2579 1719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2812 3206 5236 6101 6399 2812
Size: 394 2030 865 298 3524
Fragments arranged by size:
3524 2030 865 394 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2926 3006 5774 882
Size: 617 1427 80 2768 2219
Fragments arranged by size:
2768 2219 1427 617 80
BsaAI yAC'GTr
Cuts at: 493 1204 2483 4902 493
Size: 711 1279 2419 2702
Fragments arranged by size:
2702 2419 1279 711
BsaBI GATnn'nnATC
Cuts at: 7105 7105
Size: 7111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4918 5300 5935 275
Size: 53 83 186 936 3385 382 635 1451
Fragments arranged by size:
3385 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5544 5942 6216 6363 6774 5544
Size: 398 274 147 411 5881
Fragments arranged by size:
5881 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3664 3694 3883 3913 1178
Size: 30 165 30 2261 30 189 30 4376
Fragments arranged by size:
4376 2261 189 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 4074 1186
Size: 910 1978 4223
Fragments arranged by size:
4223 1978 910
BseYI C'CCAG_C
Cuts at: 6314 6810 6314
Size: 496 6615
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6538
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4561 5322 6350 1540
Size: 275 186 1504 1056 761 1028 2301
Fragments arranged by size:
2301 1504 1056 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2048 3574 5172 5257 6328 2048
Size: 1526 1598 85 1071 2831
Fragments arranged by size:
2831 1598 1526 1071 85
BsiWI C'GTAC_G
Cuts at: 3308 3308
Size: 7111
BsmI GAATG_Cn'
Cuts at: 2132 4622 4715 2132
Size: 2490 93 4528
Fragments arranged by size:
4528 2490 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2356 2944 3574 4430 5172 5257
Size: 399 258 308 588 630 856 742 85
Cuts at: 5257 6328 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 856 742 630 588 399 308
258 85
BspEI T'CCGG_A
Cuts at: 6774 6774
Size: 7111
BspHI T'CATG_A
Cuts at: 4344 5000 6730 4344
Size: 656 1730 4725
Fragments arranged by size:
4725 1730 656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3398 3862 3911 4466 844
Size: 2554 464 49 555 3489
Fragments arranged by size:
3489 2554 555 464 49
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 4998 1394
Size: 429 272 2903 3507
Fragments arranged by size:
3507 2903 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 5606 5780 65
Size: 1183 4358 174 1396
Fragments arranged by size:
4358 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4317 5755 5942 1700
Size: 110 2507 1438 187 2869
Fragments arranged by size:
2869 2507 1438 187 110
BsrGI T'GTAC_A
Cuts at: 96 4035 96
Size: 3939 3172
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7111
BssSI C'ACGA_G
Cuts at: 2349 2697 5171 6183 2349
Size: 348 2474 1012 3277
Fragments arranged by size:
3277 2474 1012 348
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7111
BstBI TT'CG_AA
Cuts at: 5915 5915
Size: 7111
BstEII G'GTnAC_C
Cuts at: 1184 3923 1184
Size: 2739 4372
BstXI CCAn_nnnn'nTGG
Cuts at: 3431 3431
Size: 7111
BstYI r'GATC_y
Cuts at: 1972 2686 4441 4808 5193 5210 6662 6748 6760
Size: 714 1755 367 385 17 1452 86 12
Cuts at: 6760 7106 1972
Size: 346 1977
Fragments arranged by size:
1977 1755 1452 714 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3805 513
Size: 552 2740 3819
Fragments arranged by size:
3819 2740 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6372
Fragments arranged by size:
6372 683 56
BtsI GCAGTG_nn'
Cuts at: 4627 5421 5441 4627
Size: 794 20 6297
Fragments arranged by size:
6297 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7076
DraI TTT'AAA
Cuts at: 3709 4415 3709
Size: 706 6405
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6118 809
Size: 507 4802 1802
Fragments arranged by size:
4802 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3785 4330 4558
Size: 54 1425 50 275 18 1955 545 228
Cuts at: 4558 5447 7076 8
Size: 889 1629 43
Fragments arranged by size:
1955 1629 1425 889 545 275 228 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4558 1537
Size: 275 2746 4090
Fragments arranged by size:
4090 2746 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 3147 3268 3832 5041 1353
Size: 791 1003 121 564 1209 3423
Fragments arranged by size:
3423 1209 1003 791 564 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5698 6072 6218 387
Size: 1064 138 4109 374 146 1280
Fragments arranged by size:
4109 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2895 2958 3561 3759 3891 3915
Size: 393 887 376 63 603 198 132 24
Cuts at: 3915 5174 5771 6558 6826 1239
Size: 1259 597 787 268 1524
Fragments arranged by size:
1524 1259 887 787 603 597 393 376
268 198 132 63 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3741 3741
Size: 7111
EcoO109I rG'GnC_Cy
Cuts at: 2321 2321
Size: 7111
EcoRI G'AATT_C
Cuts at: 3435 4420 4798 3435
Size: 985 378 5748
Fragments arranged by size:
5748 985 378
FspI TGC'GCA
Cuts at: 5617 5617
Size: 7111
HaeII r_GCGC'y
Cuts at: 1678 2108 2137 3826 4282 6258 1678
Size: 430 29 1689 456 1976 2531
Fragments arranged by size:
2531 1976 1689 456 430 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2462 2494 3782
Size: 32 135 32 473 32 385 32 1288
Cuts at: 3782 3814 3883 3915 5752 5784 5826 5858 1373
Size: 32 69 32 1837 32 42 32 2626
Fragments arranged by size:
2626 1837 1288 473 385 135 69 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3502 4455 4701 669
Size: 988 281 1564 953 246 3079
Fragments arranged by size:
3079 1564 988 953 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7111
HpaI GTT'AAC
Cuts at: 4701 4701
Size: 7111
KpnI G_GTAC'C
Cuts at: 4436 4436
Size: 7111
MfeI C'AATT_G
Cuts at: 4062 4710 4062
Size: 648 6463
MluI A'CGCG_T
Cuts at: 6987 6987
Size: 7111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 2173 3349 6225 6409 1305
Size: 834 34 1176 2876 184 2007
Fragments arranged by size:
2876 2007 1176 834 184 34
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3787 4332 10
Size: 54 1425 343 1955 545 2789
Fragments arranged by size:
2789 1955 1425 545 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3429 5069 5428 5587 518
Size: 648 2263 1640 359 159 2042
Fragments arranged by size:
2263 2042 1640 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2215 3581 4527 5204 6352 6597 1606
Size: 609 1366 946 677 1148 245 2120
Fragments arranged by size:
2120 1366 1148 946 677 609 245
NaeI GCC'GGC
Cuts at: 4319 4319
Size: 7111
NcoI C'CATG_G
Cuts at: 513 1065 3805 513
Size: 552 2740 3819
Fragments arranged by size:
3819 2740 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7111
NgoMIV G'CCGG_C
Cuts at: 4317 4317
Size: 7111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7111
NotI GC'GGCC_GC
Cuts at: 4558 4558
Size: 7111
NspI r_CATG'y
Cuts at: 1132 1229 3079 4469 6014 1132
Size: 97 1850 1390 1545 2229
Fragments arranged by size:
2229 1850 1545 1390 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7111
PciI A'CATG_T
Cuts at: 6010 6010
Size: 7111
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7111
PfoI T'CCnGG_A
Cuts at: 2773 2773
Size: 7111
PmeI GTTT'AAAC
Cuts at: 4415 4415
Size: 7111
PmlI CAC'GTG
Cuts at: 2483 2483
Size: 7111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5180 5212 6693 6725 5180
Size: 32 1481 32 5566
Fragments arranged by size:
5566 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2321 2321
Size: 7111
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7111
PsiI TTA'TAA
Cuts at: 2760 3768 4681 2760
Size: 1008 913 5190
Fragments arranged by size:
5190 1008 913
PstI C_TGCA'G
Cuts at: 830 4368 4463 830
Size: 3538 95 3478
Fragments arranged by size:
3538 3478 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7111
PvuII CAG'CTG
Cuts at: 2215 3581 2215
Size: 1366 5745
SalI G'TCGA_C
Cuts at: 1936 3500 4453 1936
Size: 1564 953 4594
Fragments arranged by size:
4594 1564 953
SbfI CC_TGCA'GG
Cuts at: 4463 4463
Size: 7111
ScaI AGT'ACT
Cuts at: 3262 5359 7071 3262
Size: 2097 1712 3302
Fragments arranged by size:
3302 2097 1712
SfcI C'TryA_G
Cuts at: 826 1045 2907 4274 4364 4396 4459 5594 6275
Size: 219 1862 1367 90 32 63 1135 681
Cuts at: 6275 6466 826
Size: 191 1471
Fragments arranged by size:
1862 1471 1367 1135 681 219 191 90
63 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7111
SmaI CCC'GGG
Cuts at: 1872 4438 1872
Size: 2566 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2827 3221 5215 6116 6378 820
Size: 197 926 884 394 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 884 394 262 197
SnaBI TAC'GTA
Cuts at: 493 4902 493
Size: 4409 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7111
SphI G_CATG'C
Cuts at: 1132 4469 1132
Size: 3337 3774
SspI AAT'ATT
Cuts at: 5 52 5035 5
Size: 47 4983 2081
Fragments arranged by size:
4983 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7111
StyI C'CwwG_G
Cuts at: 513 1065 2422 3805 3860 4550 513
Size: 552 1357 1383 55 690 3074
Fragments arranged by size:
3074 1383 1357 690 552 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3519 5138 5155 5308 5493 5938 325
Size: 1255 1939 1619 17 153 185 445 1498
Fragments arranged by size:
1939 1619 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3260 3320 3472
Size: 274 80 33 51 1443 1283 60 152
Cuts at: 3472 4035 5357 7069 96
Size: 563 1322 1712 138
Fragments arranged by size:
1712 1443 1322 1283 563 274 152 138
80 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7071 7103 7071
Size: 32 7079
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7111
XbaI T'CTAG_A
Cuts at: 2668 4447 2668
Size: 1779 5332
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7111
XmaI C'CCGG_G
Cuts at: 1870 4436 1870
Size: 2566 4545
XmnI GAAnn'nnTTC
Cuts at: 2858 5240 2858
Size: 2382 4729
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4919 276
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI