Restriction map for
pFN21AB9376
(Circular) (Six-base) MAPSORT of: pFN21AB9376.seq Check: 5063 from: 1 to: 5938
pFN21AB9376 5938 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 1937
Size: 1344 4594
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2412 2736 2912 3120 4001 5385 5653 1632
Size: 780 324 176 208 881 1384 268 1917
Fragments arranged by size:
1917 1384 881 780 324 268 208 176
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 4837 5814 1203
Size: 3634 977 1327
Fragments arranged by size:
3634 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 2615 1166
Size: 1449 4489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 2663 2964 3023 3681 5253 2663
Size: 301 59 658 1572 3348
Fragments arranged by size:
3348 1572 658 301 59
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 2149 2305 2403 2436 3247 3473 3625 5734
Size: 656 156 98 33 811 226 152 2109
Cuts at: 5734 1493
Size: 1697
Fragments arranged by size:
2109 1697 811 656 226 156 152 98
33
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 3263 1870
Size: 73 1320 4545
Fragments arranged by size:
4545 1320 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2596 3259 4714 618
Size: 325 217 627 809 663 1455 1842
Fragments arranged by size:
1842 1455 809 663 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2039 2046 3257 1391
Size: 648 7 1211 4072
Fragments arranged by size:
4072 1211 648 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4967
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3830 5040 3830
Size: 1210 4728
BclI T'GATC_A
Cuts at: 1548 2248 1548
Size: 700 5238
BfrBI ATG'CAT
Cuts at: 2087 2087
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4549 136
Size: 107 122 71 631 605 2877 1525
Fragments arranged by size:
2877 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 3999 5155 1790
Size: 2209 1156 2573
Fragments arranged by size:
2573 2209 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2518 2974 3669
Size: 416 53 83 399 24 1101 456 695
Cuts at: 3669 4627 442
Size: 958 1753
Fragments arranged by size:
1753 1101 958 695 456 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3045 4598 1239
Size: 1806 1553 2579
Fragments arranged by size:
2579 1806 1553
Bpu10I CC'TnA_GC
Cuts at: 2038 2038
Size: 5938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4601 882
Size: 617 3102 2219
Fragments arranged by size:
3102 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3745 4127 4762 275
Size: 53 83 186 936 2212 382 635 1451
Fragments arranged by size:
2212 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2626 2656 1178
Size: 30 165 30 1223 30 4460
Fragments arranged by size:
4460 1223 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5938
BseYI C'CCAG_C
Cuts at: 5141 5637 5141
Size: 496 5442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 1163
Size: 573 558 4807
Fragments arranged by size:
4807 573 558
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2555 3999 4084 5155 2555
Size: 1444 85 1071 3338
Fragments arranged by size:
3338 1444 1071 85
BsmI GAATG_Cn'
Cuts at: 3449 3542 3449
Size: 93 5845
BsmBI CGTCTCn'nnnn_
Cuts at: 2350 2350
Size: 5938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2046 2555 3257 3999 4084 5155
Size: 399 249 7 509 702 742 85 1071
Cuts at: 5155 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 702 509 399 249 85
7
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3293 844
Size: 2449 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3825 1394
Size: 429 2002 3507
Fragments arranged by size:
3507 2002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2695 2940 4433 4607 65
Size: 1183 1447 245 1493 174 1396
Fragments arranged by size:
1493 1447 1396 1183 245 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 2521 96
Size: 2425 3513
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstYI r'GATC_y
Cuts at: 1972 3268 3635 4020 4037 5489 5575 5587 5933
Size: 1296 367 385 17 1452 86 12 346
Cuts at: 5933 1972
Size: 1977
Fragments arranged by size:
1977 1452 1296 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2209 2712 513
Size: 552 939 205 503 3739
Fragments arranged by size:
3739 939 552 503 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 2808 3153 3454 4248 4268 2277
Size: 531 345 301 794 20 3947
Fragments arranged by size:
3947 794 531 345 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2427 3385 4274
Size: 54 1425 50 275 18 597 958 889
Cuts at: 4274 5903 8
Size: 1629 43
Fragments arranged by size:
1629 1425 958 889 597 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 3868 1353
Size: 674 1841 3423
Fragments arranged by size:
3423 1841 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2006 4525 4899 5045 387
Size: 1064 138 417 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 417 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2412 2736 2912 3045 3120 4001 4598
Size: 393 780 324 176 133 75 881 597
Cuts at: 4598 5385 5653 1239
Size: 787 268 1524
Fragments arranged by size:
1524 881 787 780 597 393 324 268
176 133 75
EcoRI G'AATT_C
Cuts at: 2403 2436 3247 3625 2403
Size: 33 811 378 4716
Fragments arranged by size:
4716 811 378 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2423 2423
Size: 5938
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 5085 1678
Size: 3407 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2330 2362 4579 4611 4653
Size: 32 135 32 758 32 2217 32 42
Cuts at: 4653 4685 1373
Size: 32 2626
Fragments arranged by size:
2626 2217 758 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2526 748
Size: 1778 4160
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 2334 3537 2334
Size: 1203 4735
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2524 5052 5236 1305
Size: 1219 2528 184 2007
Fragments arranged by size:
2528 2007 1219 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 10
Size: 54 1425 343 597 3519
Fragments arranged by size:
3519 1425 597 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 2909 3896 4255 4414 518
Size: 648 1449 294 987 359 159 2042
Fragments arranged by size:
2042 1449 987 648 359 294 159
MspA1I CmG'CkG
Cuts at: 1606 2725 2796 3098 3354 4031 5179 5424 1606
Size: 1119 71 302 256 677 1148 245 2120
Fragments arranged by size:
2120 1148 1119 677 302 256 245 71
NcoI C'CATG_G
Cuts at: 513 1065 2004 2209 2712 513
Size: 552 939 205 503 3739
Fragments arranged by size:
3739 939 552 503 205
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NsiI A_TGCA'T
Cuts at: 2089 2089
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2914 3296 4841 1132
Size: 97 1685 382 1545 2229
Fragments arranged by size:
2229 1685 1545 382 97
PasI CC'CwG_GG
Cuts at: 1348 2977 1348
Size: 1629 4309
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2663 1108
Size: 1555 4383
PfoI T'CCnGG_A
Cuts at: 2294 2294
Size: 5938
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 2187 3173 3508 2187
Size: 986 335 4617
Fragments arranged by size:
4617 986 335
PstI C_TGCA'G
Cuts at: 830 2724 3290 830
Size: 1894 566 3478
Fragments arranged by size:
3478 1894 566
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2725 2796 3098 2725
Size: 71 302 5565
Fragments arranged by size:
5565 302 71
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SfcI C'TryA_G
Cuts at: 826 1045 2720 3093 3286 4421 5102 5293 826
Size: 219 1675 373 193 1135 681 191 1471
Fragments arranged by size:
1675 1471 1135 681 373 219 193 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4042 4943 5205 820
Size: 197 926 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5938
StyI C'CwwG_G
Cuts at: 513 1065 2004 2209 2313 2712 3159 3377 513
Size: 552 939 205 104 399 447 218 3074
Fragments arranged by size:
3074 939 552 447 399 218 205 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2614 3140 3965 3982 4135 4320 4765
Size: 1255 1034 526 825 17 153 185 445
Cuts at: 4765 325
Size: 1498
Fragments arranged by size:
1498 1255 1034 825 526 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2521 4184 5896
Size: 274 80 33 51 1443 544 1663 1712
Cuts at: 5896 96
Size: 138
Fragments arranged by size:
1712 1663 1443 544 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2365 2397 5898 5930 2365
Size: 32 3501 32 2373
Fragments arranged by size:
3501 2373 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2342 2855 1316
Size: 1026 513 4399
Fragments arranged by size:
4399 1026 513
XbaI T'CTAG_A
Cuts at: 2848 3274 2848
Size: 426 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 2671 3088 4067 2671
Size: 417 979 4542
Fragments arranged by size:
4542 979 417
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI