(Circular) (Six-base) MAPSORT of: pFN21AB9348.seq Check: 6387 from: 1 to: 5524 pFN21AB9348 5524 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2867 1937 Size: 930 4594 Acc65I G'GTAC_C Cuts at: 2845 2845 Size: 5524 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 3587 4971 5239 1632 Size: 941 1014 1384 268 1917 Fragments arranged by size: 1917 1384 1014 941 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 4423 5400 1203 Size: 3220 977 1327 Fragments arranged by size: 3220 1327 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 2421 4253 2421 Size: 1832 3692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2553 1184 Size: 1369 4155 AlwNI CAG_nnn'CTG Cuts at: 3267 4839 3267 Size: 1572 3952 ApaI G_GGCC'C Cuts at: 2820 2820 Size: 5524 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2849 1870 Size: 73 906 4545 Fragments arranged by size: 4545 906 73 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2676 2845 4300 618 Size: 325 217 627 889 169 1455 1842 Fragments arranged by size: 1842 1455 889 627 325 217 169 BanII G_rGCy'C Cuts at: 1391 2514 2820 2843 1391 Size: 1123 306 23 4072 Fragments arranged by size: 4072 1123 306 23 BbeI G_GCGC'C Cuts at: 2680 2680 Size: 5524 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4553 BbvCI CC'TCA_GC Cuts at: 2541 2541 Size: 5524 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2770 2804 3715 3749 1903 Size: 34 833 34 911 34 3678 Fragments arranged by size: 3678 911 833 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 2568 1548 Size: 1020 4504 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4135 136 Size: 107 122 71 631 605 2463 1525 Fragments arranged by size: 2463 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 2820 3585 4741 1790 Size: 1030 765 1156 2573 Fragments arranged by size: 2573 1156 1030 765 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2415 3255 4213 Size: 416 53 83 399 24 998 840 958 Cuts at: 4213 442 Size: 1753 Fragments arranged by size: 1753 998 958 840 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2433 2433 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2117 2222 2252 2420 2476 2738 4184 Size: 818 60 105 30 168 56 262 1446 Cuts at: 4184 1239 Size: 2579 Fragments arranged by size: 2579 1446 818 262 168 105 60 56 30 Bpu10I CC'TnA_GC Cuts at: 2541 2541 Size: 5524 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2009 3649 4514 4812 2009 Size: 1640 865 298 2721 Fragments arranged by size: 2721 1640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2798 4187 882 Size: 617 1299 1389 2219 Fragments arranged by size: 2219 1389 1299 617 BsaAI yAC'GTr Cuts at: 493 1204 3315 493 Size: 711 2111 2702 Fragments arranged by size: 2702 2111 711 BsaBI GATnn'nnATC Cuts at: 2699 5518 2699 Size: 2819 2705 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2677 3331 3713 4348 Size: 53 83 186 936 1144 654 382 635 Cuts at: 4348 275 Size: 1451 Fragments arranged by size: 1451 1144 936 654 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2467 3957 4355 4629 4776 5187 2467 Size: 1490 398 274 147 411 2804 Fragments arranged by size: 2804 1490 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5299 Fragments arranged by size: 5299 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2335 2558 1186 Size: 928 221 223 4152 Fragments arranged by size: 4152 928 223 221 BseYI C'CCAG_C Cuts at: 2559 4727 5223 2559 Size: 2168 496 2860 Fragments arranged by size: 2860 2168 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4951 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2974 3735 4763 1540 Size: 275 186 973 761 1028 2301 Fragments arranged by size: 2301 1028 973 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 2490 2514 3585 3670 4741 2043 Size: 447 24 1071 85 1071 2826 Fragments arranged by size: 2826 1071 1071 447 85 24 BsmI GAATG_Cn' Cuts at: 3035 3128 3035 Size: 93 5431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2490 2514 2820 2843 3585 3670 Size: 399 253 447 24 306 23 742 85 Cuts at: 3670 4741 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 306 253 85 24 23 BspEI T'CCGG_A Cuts at: 2467 5187 2467 Size: 2720 2804 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2283 2879 844 Size: 1439 596 3489 Fragments arranged by size: 3489 1439 596 BsrBI CCG'CTC Cuts at: 1394 1823 3411 1394 Size: 429 1588 3507 Fragments arranged by size: 3507 1588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4168 4355 1700 Size: 110 2358 187 2869 Fragments arranged by size: 2869 2358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5524 BssSI C'ACGA_G Cuts at: 3584 4596 3584 Size: 1012 4512 BstAPI GCAn_nnn'nTGC Cuts at: 794 2047 794 Size: 1253 4271 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5524 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2160 2263 2443 2128 Size: 32 103 180 5209 Fragments arranged by size: 5209 180 103 32 BstYI r'GATC_y Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519 Size: 882 367 385 17 1452 86 12 346 Cuts at: 5519 1972 Size: 1977 Fragments arranged by size: 1977 1452 882 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2016 2016 Size: 5524 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 513 Size: 552 939 123 3910 Fragments arranged by size: 3910 939 552 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4785 Fragments arranged by size: 4785 683 56 BtsI GCAGTG_nn' Cuts at: 2349 3040 3834 3854 2349 Size: 691 794 20 4019 Fragments arranged by size: 4019 794 691 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5489 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2791 4531 809 Size: 507 1475 1740 1802 Fragments arranged by size: 1802 1740 1475 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 2751 2971 Size: 54 1425 50 275 18 224 697 220 Cuts at: 2971 3860 5489 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 697 275 224 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2971 1537 Size: 275 1159 4090 Fragments arranged by size: 4090 1159 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2057 2326 2519 3454 1353 Size: 704 269 193 935 3423 Fragments arranged by size: 3423 935 704 269 193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4111 4485 4631 387 Size: 1064 138 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2512 2512 Size: 5524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2117 2222 2252 2420 2476 2573 Size: 393 425 60 105 30 168 56 97 Cuts at: 2573 2738 3587 4184 4971 5239 1239 Size: 165 849 597 787 268 1524 Fragments arranged by size: 1524 849 787 597 425 393 268 168 165 105 97 60 56 30 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2720 2402 Size: 318 5206 EcoO109I rG'GnC_Cy Cuts at: 2020 2548 2817 2020 Size: 528 269 4727 Fragments arranged by size: 4727 528 269 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 5524 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 2680 4671 1678 Size: 1002 1991 2531 Fragments arranged by size: 2531 1991 1002 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2206 2407 2439 4165 Size: 32 135 32 602 32 201 32 1726 Cuts at: 4165 4197 4239 4271 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1726 602 201 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2868 3114 669 Size: 988 281 930 246 3079 Fragments arranged by size: 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KasI G'GCGC_C Cuts at: 2676 2676 Size: 5524 KpnI G_GTAC'C Cuts at: 2849 2849 Size: 5524 MfeI C'AATT_G Cuts at: 2437 3123 2437 Size: 686 4838 MluI A'CGCG_T Cuts at: 5400 5400 Size: 5524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 4822 1305 Size: 3333 184 2007 Fragments arranged by size: 3333 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2056 10 Size: 54 1425 343 224 3478 Fragments arranged by size: 3478 1425 343 224 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3482 3841 4000 518 Size: 648 843 1473 359 159 2042 Fragments arranged by size: 2042 1473 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2940 3617 4765 5010 1606 Size: 1334 677 1148 245 2120 Fragments arranged by size: 2120 1334 1148 677 245 NarI GG'CG_CC Cuts at: 2677 2677 Size: 5524 NcoI C'CATG_G Cuts at: 513 1065 2004 2127 513 Size: 552 939 123 3910 Fragments arranged by size: 3910 939 552 123 NdeI CA'TA_TG Cuts at: 387 2179 387 Size: 1792 3732 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2014 2882 4427 1132 Size: 97 785 868 1545 2229 Fragments arranged by size: 2229 1545 868 785 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5524 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 2183 1108 Size: 1075 4449 PfoI T'CCnGG_A Cuts at: 2715 2715 Size: 5524 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 2548 2020 Size: 528 4996 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PspOMI G'GGCC_C Cuts at: 2816 2816 Size: 5524 PstI C_TGCA'G Cuts at: 830 2876 830 Size: 2046 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 SacI G_AGCT'C Cuts at: 2514 2514 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SapI GCTCTTCn'nnn_ Cuts at: 2326 2519 2326 Size: 193 5331 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 2621 3772 5484 2621 Size: 1151 1712 2661 Fragments arranged by size: 2661 1712 1151 SexAI A'CCwGG_T Cuts at: 2481 2481 Size: 5524 SfcI C'TryA_G Cuts at: 826 1045 2286 2872 4007 4688 4879 826 Size: 219 1241 586 1135 681 191 1471 Fragments arranged by size: 1471 1241 1135 681 586 219 191 SfoI GGC'GCC Cuts at: 2678 2678 Size: 5524 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2851 1872 Size: 979 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2024 3628 4529 4791 820 Size: 197 926 81 1604 901 262 1553 Fragments arranged by size: 1604 1553 926 901 262 197 81 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2882 1132 Size: 1750 3774 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5524 StyI C'CwwG_G Cuts at: 513 1065 2004 2051 2127 2820 2963 513 Size: 552 939 47 76 693 143 3074 Fragments arranged by size: 3074 939 693 552 143 76 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 2273 3551 3568 3721 3906 4351 Size: 1255 590 103 1278 17 153 185 445 Cuts at: 4351 325 Size: 1498 Fragments arranged by size: 1498 1278 1255 590 445 185 153 103 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2205 2619 3770 Size: 274 80 33 51 1443 228 414 1151 Cuts at: 3770 5482 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1151 414 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 5492 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2849 1870 Size: 979 4545 XmnI GAAnn'nnTTC Cuts at: 3653 3653 Size: 5524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfiI SrfI SwaI XcmI