Restriction map for
pFN21AB9339
(Circular) (Six-base) MAPSORT of: pFN21AB9339.seq Check: 6266 from: 1 to: 8497
pFN21AB9339 8497 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3424 4183 3424
Size: 759 7738
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2692 4641 6307 278
Size: 53 83 186 936 1156 1949 1666 2468
Fragments arranged by size:
2468 1949 1666 1156 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3822 5840 1937
Size: 1885 2018 4594
Fragments arranged by size:
4594 2018 1885
Acc65I G'GTAC_C
Cuts at: 5818 5818
Size: 8497
AclI AA'CG_TT
Cuts at: 1563 6624 6997 1563
Size: 5061 373 3063
Fragments arranged by size:
5061 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3814 3826 4922 5400 5683 6560 7944 8212
Size: 2182 12 1096 478 283 877 1384 268
Cuts at: 8212 1632
Size: 1917
Fragments arranged by size:
2182 1917 1384 1096 877 478 283 268
12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8300
AflIII A'CryG_T
Cuts at: 1203 4836 5229 7396 8373 1203
Size: 3633 393 2167 977 1327
Fragments arranged by size:
3633 2167 1327 977 393
AgeI A'CCGG_T
Cuts at: 3656 7328 3656
Size: 3672 4825
AhdI GACnn_n'nnGTC
Cuts at: 4567 5455 7226 4567
Size: 888 1771 5838
Fragments arranged by size:
5838 1771 888
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4908 4940 4908
Size: 32 8465
AlwNI CAG_nnn'CTG
Cuts at: 3617 3727 3868 5035 5539 6240 7812 3617
Size: 110 141 1167 504 701 1572 4302
Fragments arranged by size:
4302 1572 1167 701 504 141 110
ApaI G_GGCC'C
Cuts at: 4513 4513
Size: 8497
ApaLI G'TGCA_C
Cuts at: 6554 7710 6554
Size: 1156 7341
ApoI r'AATT_y
Cuts at: 1493 2114 2273 2487 3227 3623 4790 5806 6032
Size: 621 159 214 740 396 1167 1016 226
Cuts at: 6032 6184 8293 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1167 1016 740 621 396 226
214 159 152
AseI AT'TA_AT
Cuts at: 160 1032 7051 160
Size: 872 6019 1606
Fragments arranged by size:
6019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8497
AvaI C'yCGr_G
Cuts at: 1870 1943 2949 3425 3983 4324 4413 5043 5822
Size: 73 1006 476 558 341 89 630 779
Cuts at: 5822 1870
Size: 4545
Fragments arranged by size:
4545 1006 779 630 558 476 341 89
73
BamHI G'GATC_C
Cuts at: 5827 6194 5827
Size: 367 8130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 5380 5818 7273 618
Size: 325 217 627 1148 2445 438 1455 1842
Fragments arranged by size:
2445 1842 1455 1148 627 438 325 217
BanII G_rGCy'C
Cuts at: 1391 2708 2757 2823 4196 4513 4761 4872 5130
Size: 1317 49 66 1373 317 248 111 258
Cuts at: 5130 5195 5816 1391
Size: 65 621 4072
Fragments arranged by size:
4072 1373 1317 621 317 258 248 111
66 65 49
BbeI G_GCGC'C
Cuts at: 2939 5384 2939
Size: 2445 6052
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 4915 5798 928
Size: 971 702 2314 883 3627
Fragments arranged by size:
3627 2314 971 883 702
BbvCI CC'TCA_GC
Cuts at: 2166 3267 3610 4459 5715 2166
Size: 1101 343 849 1256 4948
Fragments arranged by size:
4948 1256 1101 849 343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5428 5462 6688 6722 1903
Size: 34 3491 34 1226 34 3678
Fragments arranged by size:
3678 3491 1226 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6389 7599 6389
Size: 1210 7287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4047 5477 7108
Size: 107 122 71 631 605 2375 1430 1631
Cuts at: 7108 136
Size: 1525
Fragments arranged by size:
2375 1631 1525 1430 631 605 122 107
71
BglII A'GATC_T
Cuts at: 8492 8492
Size: 8497
BlpI GC'TnA_GC
Cuts at: 2500 3125 4949 5124 5914 2500
Size: 625 1824 175 790 5083
Fragments arranged by size:
5083 1824 790 625 175
Bme1580I G_kGCm'C
Cuts at: 1790 2365 4065 4513 6558 7714 1790
Size: 575 1700 448 2045 1156 2573
Fragments arranged by size:
2573 2045 1700 1156 575 448
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2712 2808 5200
Size: 416 53 83 399 24 1295 96 2392
Cuts at: 5200 5248 6228 7186 442
Size: 48 980 958 1753
Fragments arranged by size:
2392 1753 1295 980 958 416 399 96
83 53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8497
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3754 4810 3754
Size: 1056 7441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2354 2438 2471 2977 3218 3749 4553
Size: 1037 78 84 33 506 241 531 804
Cuts at: 4553 5008 5417 7157 1239
Size: 455 409 1740 2579
Fragments arranged by size:
2579 1740 1037 804 531 506 455 409
241 84 78 33
Bpu10I CC'TnA_GC
Cuts at: 2166 2324 3267 3287 3610 4002 4459 5715 2166
Size: 158 943 20 323 392 457 1256 4948
Fragments arranged by size:
4948 1256 943 457 392 323 158 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4595 6622 7487 7785 4595
Size: 2027 865 298 5307
Fragments arranged by size:
5307 2027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2310 3327 7160 882
Size: 617 811 1017 3833 2219
Fragments arranged by size:
3833 2219 1017 811 617
BsaAI yAC'GTr
Cuts at: 493 1204 6288 493
Size: 711 5084 2702
Fragments arranged by size:
5084 2702 711
BsaBI GATnn'nnATC
Cuts at: 8491 8491
Size: 8497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2689 2936 3770 4473
Size: 53 83 186 936 1156 247 834 703
Cuts at: 4473 4638 5381 6304 6686 7321 275
Size: 165 743 923 382 635 1451
Fragments arranged by size:
1451 1156 936 923 834 743 703 635
382 247 186 165 83 53
BsaWI w'CCGG_w
Cuts at: 3656 4968 5244 6930 7328 7602 7749 8160 3656
Size: 1312 276 1686 398 274 147 411 3993
Fragments arranged by size:
3993 1686 1312 411 398 276 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3305 3335 3405 3435 5330
Size: 30 165 30 1902 30 70 30 1895
Cuts at: 5330 5360 1178
Size: 30 4315
Fragments arranged by size:
4315 1902 1895 165 70 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2813 2856 3669 3800 3928 4141 4412
Size: 1165 462 43 813 131 128 213 271
Cuts at: 4412 4431 4565 4769 5198 5732 1186
Size: 19 134 204 429 534 3951
Fragments arranged by size:
3951 1165 813 534 462 429 271 213
204 134 131 128 43 19
BseYI C'CCAG_C
Cuts at: 2746 3369 4232 4430 7700 8196 2746
Size: 623 863 198 3270 496 3047
Fragments arranged by size:
3270 3047 863 623 496 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 3881 5422 1163
Size: 573 756 1389 1541 4238
Fragments arranged by size:
4238 1541 1389 756 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5947 6708 7736 1540
Size: 275 186 3946 761 1028 2301
Fragments arranged by size:
3946 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2757 2823 3179 3542 3786 4196 4761 4872 4968
Size: 66 356 363 244 410 565 111 96
Cuts at: 4968 5130 5220 6558 6643 7714 2757
Size: 162 90 1338 85 1071 3540
Fragments arranged by size:
3540 1338 1071 565 410 363 356 244
162 111 96 90 85 66
BsmI GAATG_Cn'
Cuts at: 2877 4718 6008 6101 2877
Size: 1841 1290 93 5273
Fragments arranged by size:
5273 1841 1290 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2700 4027 4488 2700
Size: 1327 461 6709
Fragments arranged by size:
6709 1327 461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2365 2708 2757 2823 3179 3542 3786
Size: 399 575 343 49 66 356 363 244
Cuts at: 3786 4065 4196 4513 4761 4872 4968 5130 5195
Size: 279 131 317 248 111 96 162 65
Cuts at: 5195 5220 5816 6558 6643 7714 1391
Size: 25 596 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 596 575 399 363 356
343 317 279 248 244 162 131 111
96 85 66 65 49 25
BspEI T'CCGG_A
Cuts at: 4968 8160 4968
Size: 3192 5305
BspHI T'CATG_A
Cuts at: 5313 6386 8116 5313
Size: 1073 1730 5694
Fragments arranged by size:
5694 1730 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3424 3540 4183 5852 844
Size: 2580 116 643 1669 3489
Fragments arranged by size:
3489 2580 1669 643 116
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 3493 4001 6384 1394
Size: 429 187 1483 508 2383 3507
Fragments arranged by size:
3507 2383 1483 508 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2909 4945 6992 7166 65
Size: 1183 1661 2036 2047 174 1396
Fragments arranged by size:
2047 2036 1661 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3656 4041 4220 4426 7141 7328 1700
Size: 110 1846 385 179 206 2715 187 2869
Fragments arranged by size:
2869 2715 1846 385 206 187 179 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8497
BssSI C'ACGA_G
Cuts at: 6557 7569 6557
Size: 1012 7485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5634 794
Size: 4840 3657
BstBI TT'CG_AA
Cuts at: 7301 7301
Size: 8497
BstEII G'GTnAC_C
Cuts at: 1184 3659 1184
Size: 2475 6022
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 3077 4240 2293
Size: 784 1163 6550
Fragments arranged by size:
6550 1163 784
BstYI r'GATC_y
Cuts at: 1972 2251 2781 4453 4649 4896 5827 6194 6579
Size: 279 530 1672 196 247 931 367 385
Cuts at: 6579 6596 8048 8134 8146 8492 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1672 1452 931 530 385 367 346
279 247 196 86 17 12
BstZ17I GTA'TAC
Cuts at: 3823 3823
Size: 8497
Bsu36I CC'TnA_GG
Cuts at: 5151 5151
Size: 8497
BtgI C'CryG_G
Cuts at: 513 1065 2835 4215 4239 4642 513
Size: 552 1770 1380 24 403 4368
Fragments arranged by size:
4368 1770 1380 552 403 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7758
Fragments arranged by size:
7758 683 56
BtsI GCAGTG_nn'
Cuts at: 3179 4000 4507 6013 6807 6827 3179
Size: 821 507 1506 794 20 4849
Fragments arranged by size:
4849 1506 821 794 507 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2892 2927 529
Size: 35 2328 35 6099
Fragments arranged by size:
6099 2328 35 35
DraI TTT'AAA
Cuts at: 5801 5801
Size: 8497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2803 7504 809
Size: 507 1487 4701 1802
Fragments arranged by size:
4701 1802 1487 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3361 4218 4384
Size: 54 1425 50 275 18 1531 857 166
Cuts at: 4384 5355 5427 5944 6833 8462 8
Size: 971 72 517 889 1629 43
Fragments arranged by size:
1629 1531 1425 971 889 857 517 275
166 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5944 1537
Size: 275 4132 4090
Fragments arranged by size:
4132 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2092 2515 2838 4159 4887 5135 5183 5781
Size: 739 423 323 1321 728 248 48 598
Cuts at: 5781 6427 1353
Size: 646 3423
Fragments arranged by size:
3423 1321 739 728 646 598 423 323
248 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3476 4119 4131 4916 7084 7458
Size: 1064 138 1887 643 12 785 2168 374
Cuts at: 7458 7604 387
Size: 146 1280
Fragments arranged by size:
2168 1887 1280 1064 785 643 374 146
138 12
EcoICRI GAG'CTC
Cuts at: 2755 2821 4194 4759 4870 5128 2755
Size: 66 1373 565 111 258 6124
Fragments arranged by size:
6124 1373 565 258 111 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2354 2438 2471 2977 3218 3749
Size: 393 644 78 84 33 506 241 531
Cuts at: 3749 3814 3826 4553 4922 5008 5400 5417 5683
Size: 65 12 727 369 86 392 17 266
Cuts at: 5683 6560 7157 7944 8212 1239
Size: 877 597 787 268 1524
Fragments arranged by size:
1524 877 787 727 644 597 531 506
393 392 369 268 266 241 86 84
78 65 33 17 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3815 4985 5489 5714 3815
Size: 1170 504 225 6598
Fragments arranged by size:
6598 1170 504 225
EcoO109I rG'GnC_Cy
Cuts at: 3529 4006 4086 4509 4510 4737 5067 5422 5536
Size: 477 80 423 1 227 330 355 114
Cuts at: 5536 5722 3529
Size: 186 6304
Fragments arranged by size:
6304 477 423 355 330 227 186 114
80 1
EcoRI G'AATT_C
Cuts at: 2487 3227 5806 6184 2487
Size: 740 2579 378 4800
Fragments arranged by size:
4800 2579 740 378
FspI TGC'GCA
Cuts at: 7003 7003
Size: 8497
HaeII r_GCGC'y
Cuts at: 1678 2939 5384 7644 1678
Size: 1261 2445 2260 2531
Fragments arranged by size:
2531 2445 2260 1261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3131 3163 3305 3337 3503
Size: 32 135 32 1559 32 142 32 166
Cuts at: 3503 3535 4553 4585 5441 5473 7138 7170 7212
Size: 32 1018 32 856 32 1665 32 42
Cuts at: 7212 7244 1373
Size: 32 2626
Fragments arranged by size:
2626 1665 1559 1018 856 166 142 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2814 3034 3654 4887 5841 6087
Size: 988 281 876 220 620 1233 954 246
Cuts at: 6087 669
Size: 3079
Fragments arranged by size:
3079 1233 988 954 876 620 281 246
220
HindIII A'AGCT_T
Cuts at: 748 4119 748
Size: 3371 5126
HpaI GTT'AAC
Cuts at: 6087 6087
Size: 8497
KasI G'GCGC_C
Cuts at: 2935 5380 2935
Size: 2445 6052
KpnI G_GTAC'C
Cuts at: 5822 5822
Size: 8497
MfeI C'AATT_G
Cuts at: 2400 6096 2400
Size: 3696 4801
MluI A'CGCG_T
Cuts at: 8373 8373
Size: 8497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2723 2986 4527 7611 7795 1305
Size: 1418 263 1541 3084 184 2007
Fragments arranged by size:
3084 2007 1541 1418 263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4386 5357 10
Size: 54 1425 343 2554 971 3150
Fragments arranged by size:
3150 2554 1425 971 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2882 3075 5088 5255 6455 6814 6973
Size: 648 1716 193 2013 167 1200 359 159
Cuts at: 6973 518
Size: 2042
Fragments arranged by size:
2042 2013 1716 1200 648 359 193 167
159
MspA1I CmG'CkG
Cuts at: 1606 2165 3145 3349 3901 3907 4463 4470 4644
Size: 559 980 204 552 6 556 7 174
Cuts at: 4644 5913 6590 7738 7983 1606
Size: 1269 677 1148 245 2120
Fragments arranged by size:
2120 1269 1148 980 677 559 556 552
245 204 174 7 6
NaeI GCC'GGC
Cuts at: 4043 4043
Size: 8497
NarI GG'CG_CC
Cuts at: 2936 5381 2936
Size: 2445 6052
NcoI C'CATG_G
Cuts at: 513 1065 4215 513
Size: 552 3150 4795
Fragments arranged by size:
4795 3150 552
NdeI CA'TA_TG
Cuts at: 387 3076 387
Size: 2689 5808
NgoMIV G'CCGG_C
Cuts at: 4041 4041
Size: 8497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8497
NotI GC'GGCC_GC
Cuts at: 5944 5944
Size: 8497
NspI r_CATG'y
Cuts at: 1132 1229 3709 3721 5233 5632 5662 5855 7400
Size: 97 2480 12 1512 399 30 193 1545
Cuts at: 7400 1132
Size: 2229
Fragments arranged by size:
2480 2229 1545 1512 399 193 97 30
12
PasI CC'CwG_GG
Cuts at: 1348 3392 4478 1348
Size: 2044 1086 5367
Fragments arranged by size:
5367 2044 1086
PciI A'CATG_T
Cuts at: 5229 7396 5229
Size: 2167 6330
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3332 4430 5035 5601 1108
Size: 2224 1098 605 566 4004
Fragments arranged by size:
4004 2224 1098 605 566
PfoI T'CCnGG_A
Cuts at: 3179 4980 3179
Size: 1801 6696
PmeI GTTT'AAAC
Cuts at: 5801 5801
Size: 8497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5277 5309 6566 6598 8079 8111 5277
Size: 32 1257 32 1481 32 5663
Fragments arranged by size:
5663 1481 1257 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3529 4086 4737 5067 5536 3529
Size: 557 651 330 469 6490
Fragments arranged by size:
6490 651 557 469 330
PshAI GACnn'nnGTC
Cuts at: 1613 2692 1613
Size: 1079 7418
PsiI TTA'TAA
Cuts at: 5681 6067 5681
Size: 386 8111
PspOMI G'GGCC_C
Cuts at: 4509 4509
Size: 8497
PspXI vC'TCGA_Gb
Cuts at: 4324 4324
Size: 8497
PstI C_TGCA'G
Cuts at: 830 3110 3479 3537 3912 4776 5535 5849 830
Size: 2280 369 58 375 864 759 314 3478
Fragments arranged by size:
3478 2280 864 759 375 369 314 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8497
PvuII CAG'CTG
Cuts at: 2165 3145 3349 3907 2165
Size: 980 204 558 6755
Fragments arranged by size:
6755 980 558 204
SacI G_AGCT'C
Cuts at: 2757 2823 4196 4761 4872 5130 2757
Size: 66 1373 565 111 258 6124
Fragments arranged by size:
6124 1373 565 258 111 66
SacII CC_GC'GG
Cuts at: 4645 4645
Size: 8497
SalI G'TCGA_C
Cuts at: 1936 5839 1936
Size: 3903 4594
SapI GCTCTTCn'nnn_
Cuts at: 5135 5183 5135
Size: 48 8449
SbfI CC_TGCA'GG
Cuts at: 5535 5849 5535
Size: 314 8183
ScaI AGT'ACT
Cuts at: 5435 6745 8457 5435
Size: 1310 1712 5475
Fragments arranged by size:
5475 1712 1310
SfcI C'TryA_G
Cuts at: 826 1045 2518 3106 3475 3533 3908 4732 4772
Size: 219 1473 588 369 58 375 824 40
Cuts at: 4772 5531 5845 6980 7661 7852 826
Size: 759 314 1135 681 191 1471
Fragments arranged by size:
1473 1471 1135 824 759 681 588 375
369 314 219 191 58 40
SfoI GGC'GCC
Cuts at: 2937 5382 2937
Size: 2445 6052
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8497
SmaI CCC'GGG
Cuts at: 1872 5045 5824 1872
Size: 3173 779 4545
Fragments arranged by size:
4545 3173 779
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4324 4413 4610 6601 7502 7764
Size: 197 926 2381 89 197 1991 901 262
Cuts at: 7764 820
Size: 1553
Fragments arranged by size:
2381 1991 1553 926 901 262 197 197
89
SnaBI TAC'GTA
Cuts at: 493 6288 493
Size: 5795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8497
SphI G_CATG'C
Cuts at: 1132 5632 5855 1132
Size: 4500 223 3774
Fragments arranged by size:
4500 3774 223
SspI AAT'ATT
Cuts at: 5 52 6421 5
Size: 47 6369 2081
Fragments arranged by size:
6369 2081 47
StuI AGG'CCT
Cuts at: 1517 3914 1517
Size: 2397 6100
StyI C'CwwG_G
Cuts at: 513 1065 2247 4215 5936 513
Size: 552 1182 1968 1721 3074
Fragments arranged by size:
3074 1968 1721 1182 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 2932 3620 6524 6541 6694 6879
Size: 1255 560 792 688 2904 17 153 185
Cuts at: 6879 7324 325
Size: 445 1498
Fragments arranged by size:
2904 1498 1255 792 688 560 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4707 4776 5433
Size: 274 80 33 51 1443 2730 69 657
Cuts at: 5433 6743 8455 96
Size: 1310 1712 138
Fragments arranged by size:
2730 1712 1443 1310 657 274 138 80
69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3405 3437 4106 4138 5489 5521 8457 8489 3405
Size: 32 669 32 1351 32 2936 32 3413
Fragments arranged by size:
3413 2936 1351 669 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8497
XbaI T'CTAG_A
Cuts at: 5833 5833
Size: 8497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2104 2290 2446 2464 3077 3353 5032 5392 2104
Size: 186 156 18 613 276 1679 360 5209
Fragments arranged by size:
5209 1679 613 360 276 186 156 18
XhoI C'TCGA_G
Cuts at: 1943 4324 4413 1943
Size: 2381 89 6027
Fragments arranged by size:
6027 2381 89
XmaI C'CCGG_G
Cuts at: 1870 5043 5822 1870
Size: 3173 779 4545
Fragments arranged by size:
4545 3173 779
XmnI GAAnn'nnTTC
Cuts at: 4838 5339 6626 4838
Size: 501 1287 6709
Fragments arranged by size:
6709 1287 501
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2690 4639 6305 276
Size: 53 83 186 936 1156 1949 1666 2468
Fragments arranged by size:
2468 1949 1666 1156 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PsrI
RsrII SanDI SexAI SfiI SrfI SwaI