Restriction map for
pFN21AB9297
(Circular) (Six-base) MAPSORT of: pFN21AB9297.seq Check: 9516 from: 1 to: 5647
pFN21AB9297 5647 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2530 2530
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2961 3710 5094 5362 1632
Size: 422 907 749 1384 268 1917
Fragments arranged by size:
1917 1384 907 749 422 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 2625 4546 5523 1203
Size: 1422 1921 977 1327
Fragments arranged by size:
1921 1422 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2314 3390 4962 2314
Size: 1076 1572 2999
Fragments arranged by size:
2999 1572 1076
ApaI G_GGCC'C
Cuts at: 2330 2330
Size: 5647
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2803 2912 2956 3182 3334 5443 1493
Size: 1310 109 44 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 226 152 109 44
AseI AT'TA_AT
Cuts at: 160 1032 2798 4201 160
Size: 872 1766 1403 1606
Fragments arranged by size:
1766 1606 1403 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 2323 2921 2972 1870
Size: 73 343 37 598 51 4545
Fragments arranged by size:
4545 598 343 73 51 37
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2968 4423 618
Size: 325 217 627 1181 1455 1842
Fragments arranged by size:
1842 1455 1181 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2330 2507 2966 1391
Size: 939 177 459 4072
Fragments arranged by size:
4072 939 459 177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2062 928
Size: 971 163 4513
Fragments arranged by size:
4513 971 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 2076 1548
Size: 528 5119
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2655 5642 2655
Size: 2987 2660
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2330 3708 4864 1790
Size: 540 1378 1156 2573
Fragments arranged by size:
2573 1378 1156 540
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 3378 4336
Size: 416 53 83 399 24 876 1085 958
Cuts at: 4336 442
Size: 1753
Fragments arranged by size:
1753 1085 958 876 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2336 4307 1239
Size: 806 291 1971 2579
Fragments arranged by size:
2579 1971 806 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 3772 4637 4935 2213
Size: 1559 865 298 2925
Fragments arranged by size:
2925 1559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2628 3438 493
Size: 711 1424 810 2702
Fragments arranged by size:
2702 1424 810 711
BsaBI GATnn'nnATC
Cuts at: 2474 2703 5641 2474
Size: 229 2938 2480
Fragments arranged by size:
2938 2480 229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2257 4080 4478 4752 4899 5310 2257
Size: 1823 398 274 147 411 2594
Fragments arranged by size:
2594 1823 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2239 2269 1178
Size: 30 165 30 836 30 4556
Fragments arranged by size:
4556 836 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 1186
Size: 1294 4353
BseYI C'CCAG_C
Cuts at: 4850 5346 4850
Size: 496 5151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5074
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2507 3708 3793 4864 2507
Size: 1201 85 1071 3290
Fragments arranged by size:
3290 1201 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2507 2966 3708 3793 4864 1391
Size: 399 540 177 459 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 459 399 177 85
BspEI T'CCGG_A
Cuts at: 2257 5310 2257
Size: 3053 2594
BspHI T'CATG_A
Cuts at: 2073 3536 5266 2073
Size: 1463 1730 2454
Fragments arranged by size:
2454 1730 1463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2530 3002 844
Size: 1686 472 3489
Fragments arranged by size:
3489 1686 472
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 2040 2599 96
Size: 1944 559 3144
Fragments arranged by size:
3144 1944 559
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 2462 3707 4719 2462
Size: 1245 1012 3390
Fragments arranged by size:
3390 1245 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 794
Size: 2021 3626
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2655 2977 3344 3729 3746 5198 5284 5296
Size: 683 322 367 385 17 1452 86 12
Cuts at: 5296 5642 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 683 385 367 346 322 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2091 2524 513
Size: 552 1026 433 3636
Fragments arranged by size:
3636 1026 552 433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 3163 3957 3977 3163
Size: 794 20 4833
Fragments arranged by size:
4833 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2754 2951 2754
Size: 197 5450
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2571 3094
Size: 54 1425 50 275 18 264 477 523
Cuts at: 3094 3983 5612 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 523 477 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3577 1353
Size: 2224 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2505 2505
Size: 5647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2054 2336 2961 3710 4307 5094
Size: 393 413 9 282 625 749 597 787
Cuts at: 5094 5362 1239
Size: 268 1524
Fragments arranged by size:
1524 787 749 625 597 413 393 282
268 9
EcoO109I rG'GnC_Cy
Cuts at: 2311 2311
Size: 5647
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2091 4794 1678
Size: 413 2703 2531
Fragments arranged by size:
2703 2531 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 4288 4320 4362
Size: 32 135 32 557 32 2127 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 2127 557 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2762 748
Size: 2014 3633
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 10
Size: 54 1425 343 264 3561
Fragments arranged by size:
3561 1425 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3605 3964 4123 518
Size: 648 2439 359 159 2042
Fragments arranged by size:
2439 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2277 3063 3740 4888 5133 1606
Size: 671 786 677 1148 245 2120
Fragments arranged by size:
2120 1148 786 677 671 245
NcoI C'CATG_G
Cuts at: 513 1065 2091 513
Size: 552 1026 4069
Fragments arranged by size:
4069 1026 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2113 2579 1348
Size: 765 466 4416
Fragments arranged by size:
4416 765 466
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 2646 1108
Size: 1206 332 4109
Fragments arranged by size:
4109 1206 332
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 2721 3217 2721
Size: 496 5151
PspOMI G'GGCC_C
Cuts at: 2326 2326
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SacI G_AGCT'C
Cuts at: 2507 2507
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2493 2995 4130 4811 5002 826
Size: 219 1448 502 1135 681 191 1471
Fragments arranged by size:
1471 1448 1135 681 502 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 3751 4652 4914 820
Size: 197 926 285 1523 901 262 1553
Fragments arranged by size:
1553 1523 926 901 285 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2091 3086 513
Size: 552 1026 995 3074
Fragments arranged by size:
3074 1026 995 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2312 2420 3674 3691 3844 4029 4474
Size: 1255 732 108 1254 17 153 185 445
Cuts at: 4474 325
Size: 1498
Fragments arranged by size:
1498 1255 1254 732 445 185 153 108
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2040 2264 2412
Size: 274 80 33 51 1443 63 224 148
Cuts at: 2412 2599 3893 5605 96
Size: 187 1294 1712 138
Fragments arranged by size:
1712 1443 1294 274 224 187 148 138
80 63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2239 2271 5607 5639 2239
Size: 32 3336 32 2247
Fragments arranged by size:
3336 2247 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 2378 3776 2378
Size: 1398 4249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI