Restriction map for
pFN21AB9290
(Circular) (Six-base) MAPSORT of: pFN21AB9290.seq Check: 790 from: 1 to: 5596
pFN21AB9290 5596 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 2484 3723 4096 1563
Size: 921 1239 373 3063
Fragments arranged by size:
3063 1239 921 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2226 2702 3659 5043 5311 1632
Size: 521 73 476 957 1384 268 1917
Fragments arranged by size:
1917 1384 957 521 476 268 73
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 2121
Size: 32 5564
AlwNI CAG_nnn'CTG
Cuts at: 2059 2467 2602 3339 4911 2059
Size: 408 135 737 1572 2744
Fragments arranged by size:
2744 1572 737 408 135
ApaLI G'TGCA_C
Cuts at: 2118 3653 4809 2118
Size: 1535 1156 2905
Fragments arranged by size:
2905 1535 1156
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2445 2921 1870
Size: 73 502 476 4545
Fragments arranged by size:
4545 502 476 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2795 2917 4372 618
Size: 325 217 627 1008 122 1455 1842
Fragments arranged by size:
1842 1455 1008 627 325 217 122
BanII G_rGCy'C
Cuts at: 1391 2774 2915 1391
Size: 1383 141 4072
Fragments arranged by size:
4072 1383 141
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2172 5591 2172
Size: 3419 2177
BlpI GC'TnA_GC
Cuts at: 2391 3013 2391
Size: 622 4974
Bme1580I G_kGCm'C
Cuts at: 1790 2122 2566 3657 4813 1790
Size: 332 444 1091 1156 2573
Fragments arranged by size:
2573 1156 1091 444 332
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2215 2565 3327
Size: 416 53 83 399 24 798 350 762
Cuts at: 3327 4285 442
Size: 958 1753
Fragments arranged by size:
1753 958 798 762 416 399 350 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2189 2199 2269 2549 2870 4256 1239
Size: 887 63 10 70 280 321 1386 2579
Fragments arranged by size:
2579 1386 887 321 280 70 63 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2567 3387 493
Size: 711 1363 820 2702
Fragments arranged by size:
2702 1363 820 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2416 4029 4427 4701 4848 5259 2416
Size: 1613 398 274 147 411 2753
Fragments arranged by size:
2753 1613 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2048 2078 2121 2151 2323
Size: 30 165 30 645 30 43 30 172
Cuts at: 2323 2353 2414 2444 1178
Size: 30 61 30 4330
Fragments arranged by size:
4330 645 172 165 61 43 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 1186
Size: 943 4653
BseYI C'CCAG_C
Cuts at: 2602 2697 4799 5295 2602
Size: 95 2102 496 2903
Fragments arranged by size:
2903 2102 496 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 1163
Size: 573 432 4591
Fragments arranged by size:
4591 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2734 3046 3807 4835 1540
Size: 275 186 733 312 761 1028 2301
Fragments arranged by size:
2301 1028 761 733 312 275 186
BsiHKAI G_wGCw'C
Cuts at: 2122 2232 2392 2808 2856 3657 3742 4813 2122
Size: 110 160 416 48 801 85 1071 2905
Fragments arranged by size:
2905 1071 801 416 160 110 85 48
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2232 2392 2566 2774 2808 2856
Size: 399 332 110 160 174 208 34 48
Cuts at: 2856 2915 3657 3742 4813 1391
Size: 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 332 208 174 160
110 85 59 48 34
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 2951 844
Size: 1757 350 3489
Fragments arranged by size:
3489 1757 350
BsrBI CCG'CTC
Cuts at: 1394 1823 2736 3483 1394
Size: 429 913 747 3507
Fragments arranged by size:
3507 913 747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2673 2729 4240 4427 1700
Size: 110 863 56 1511 187 2869
Fragments arranged by size:
2869 1511 863 187 110 56
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2848 2011
Size: 837 4759
BstYI r'GATC_y
Cuts at: 1972 2172 2926 3293 3678 3695 5147 5233 5245
Size: 200 754 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 754 385 367 346 200 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2863 2863
Size: 5596
BtgI C'CryG_G
Cuts at: 513 1065 2709 513
Size: 552 1644 3400
Fragments arranged by size:
3400 1644 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 3112 3906 3926 2120
Size: 992 794 20 3790
Fragments arranged by size:
3790 992 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2731 3043 3932
Size: 54 1425 50 275 18 901 312 889
Cuts at: 3932 5561 8
Size: 1629 43
Fragments arranged by size:
1629 1425 901 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2731 3043 1537
Size: 275 919 312 4090
Fragments arranged by size:
4090 919 312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3526 1353
Size: 2173 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2153 2189 2199 2226 2269 2549
Size: 393 494 27 36 10 27 43 280
Cuts at: 2549 2702 2870 3659 4256 5043 5311 1239
Size: 153 168 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 597 494 393 280 268
168 153 43 36 27 27 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2193 2362 2765 2051
Size: 142 169 403 4882
Fragments arranged by size:
4882 403 169 142
EcoO109I rG'GnC_Cy
Cuts at: 2186 2210 2217 2255 2845 2186
Size: 24 7 38 590 4937
Fragments arranged by size:
4937 590 38 24 7
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 5596
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2414 2446 4237
Size: 32 135 32 476 32 334 32 1791
Cuts at: 4237 4269 4311 4343 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1791 476 334 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2291 2940 3186 669
Size: 988 281 353 649 246 3079
Fragments arranged by size:
3079 988 649 353 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 2506 3195 2506
Size: 689 4907
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3774
Fragments arranged by size:
3774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2331 2438 3554 3913 4072 518
Size: 648 843 322 107 1116 359 159 2042
Fragments arranged by size:
2042 1116 843 648 359 322 159 107
MspA1I CmG'CkG
Cuts at: 1606 2167 2885 3012 3689 4837 5082 1606
Size: 561 718 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 718 677 561 245 127
NcoI C'CATG_G
Cuts at: 513 1065 2709 513
Size: 552 1644 3400
Fragments arranged by size:
3400 1644 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2602 1108
Size: 1494 4102
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PmlI CAC'GTG
Cuts at: 2567 2567
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2121 2153 3665 3697 5178 5210 2121
Size: 32 1512 32 1481 32 2507
Fragments arranged by size:
2507 1512 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2186 2210 2217 2255 2186
Size: 24 7 38 5527
Fragments arranged by size:
5527 38 24 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2167 2885 2167
Size: 718 4878
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SexAI A'CCwGG_T
Cuts at: 2496 2496
Size: 5596
SfcI C'TryA_G
Cuts at: 826 1045 2944 4079 4760 4951 826
Size: 219 1899 1135 681 191 1471
Fragments arranged by size:
1899 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2447 2923 1872
Size: 575 476 4545
Fragments arranged by size:
4545 575 476
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2709 2766 2841 3035 513
Size: 552 1644 57 75 194 3074
Fragments arranged by size:
3074 1644 552 194 75 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 2105 2689 2722 3623 3640 3793
Size: 1255 489 36 584 33 901 17 153
Cuts at: 3793 3978 4423 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 901 584 489 445 185 153
36 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2302 2323 2334 2355 2697 2729 5556 5588 2302
Size: 21 11 21 342 32 2827 32 2310
Fragments arranged by size:
2827 2310 342 32 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2074
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2445 2921 1870
Size: 575 476 4545
Fragments arranged by size:
4545 575 476
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI