Restriction map for
pFN21AB9279
(Circular) (Six-base) MAPSORT of: pFN21AB9279.seq Check: 3404 from: 1 to: 5638
pFN21AB9279 5638 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 3701 5085 5353 1632
Size: 525 1544 1384 268 1917
Fragments arranged by size:
1917 1544 1384 525 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2075 4537 5514 1203
Size: 872 2462 977 1327
Fragments arranged by size:
2462 1327 977 872
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2024 1166
Size: 858 4780
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2052 1184
Size: 868 4770
AlwNI CAG_nnn'CTG
Cuts at: 2573 3381 4953 2573
Size: 808 1572 3258
Fragments arranged by size:
3258 1572 808
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2789 5633 2789
Size: 2844 2794
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 3699 4855 1790
Size: 1909 1156 2573
Fragments arranged by size:
2573 1909 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2699 1056
Size: 1643 3995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 4298 1239
Size: 942 2117 2579
Fragments arranged by size:
2579 2117 942
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2343 3763 4628 4926 2343
Size: 1420 865 298 3055
Fragments arranged by size:
3055 1420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2844 4301 882
Size: 617 1345 1457 2219
Fragments arranged by size:
2219 1457 1345 617
BsaAI yAC'GTr
Cuts at: 493 1204 2768 3429 493
Size: 711 1564 661 2702
Fragments arranged by size:
2702 1564 711 661
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2154 2184 1178
Size: 30 165 30 751 30 4632
Fragments arranged by size:
4632 751 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 4841 5337 4841
Size: 496 5142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5065
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2957 3699 3784 4855 1391
Size: 399 1167 742 85 1071 2174
Fragments arranged by size:
2174 1167 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2378 2993 844
Size: 1534 615 3489
Fragments arranged by size:
3489 1534 615
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2200 2388 4133 4307 65
Size: 1183 952 188 1745 174 1396
Fragments arranged by size:
1745 1396 1183 952 188 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2899 2269
Size: 630 5008
BstYI r'GATC_y
Cuts at: 1972 2789 2968 3335 3720 3737 5189 5275 5287
Size: 817 179 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 817 385 367 346 179 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2154 2461 513
Size: 552 1089 307 3690
Fragments arranged by size:
3690 1089 552 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2901 3085 3974
Size: 54 1425 50 275 18 1071 184 889
Cuts at: 3974 5603 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1071 889 275 184 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2202 2649 2766 3568 1353
Size: 849 447 117 802 3423
Fragments arranged by size:
3423 849 802 447 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2181 3701 4298 5085 5353 1239
Size: 393 525 24 1520 597 787 268 1524
Fragments arranged by size:
1524 1520 787 597 525 393 268 24
EcoO109I rG'GnC_Cy
Cuts at: 2231 2258 2231
Size: 27 5611
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2154 2186 2642 2674 2848
Size: 32 135 32 582 32 456 32 174
Cuts at: 2848 2880 4279 4311 4353 4385 1373
Size: 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1399 582 456 174 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 2982 3228 669
Size: 988 281 235 809 246 3079
Fragments arranged by size:
3079 988 809 281 246 235
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2903 10
Size: 54 1425 343 1071 2745
Fragments arranged by size:
2745 1425 1071 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2897 3596 3955 4114 518
Size: 648 858 873 699 359 159 2042
Fragments arranged by size:
2042 873 858 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2378 2463 3054 3731 4879 5124 1606
Size: 540 232 85 591 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 591 540 245 232 85
NcoI C'CATG_G
Cuts at: 513 1065 2154 513
Size: 552 1089 3997
Fragments arranged by size:
3997 1089 552
NdeI CA'TA_TG
Cuts at: 387 2367 2554 387
Size: 1980 187 3471
Fragments arranged by size:
3471 1980 187
NheI G'CTAG_C
Cuts at: 1052 2695 1052
Size: 1643 3995
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2079 2545 2996 4541 1132
Size: 97 850 466 451 1545 2229
Fragments arranged by size:
2229 1545 850 466 451 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 2075 4537 2075
Size: 2462 3176
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PfoI T'CCnGG_A
Cuts at: 2188 2188
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2107 1613
Size: 494 5144
PsiI TTA'TAA
Cuts at: 2429 3208 2429
Size: 779 4859
PstI C_TGCA'G
Cuts at: 830 2109 2990 830
Size: 1279 881 3478
Fragments arranged by size:
3478 1279 881
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2146 2378 2146
Size: 232 5406
SacII CC_GC'GG
Cuts at: 2464 2464
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2105 2730 2986 4121 4802 4993 826
Size: 219 1060 625 256 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 681 625 256 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2358 3742 4643 4905 820
Size: 197 926 415 1384 901 262 1553
Fragments arranged by size:
1553 1384 926 901 415 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2154 3077 513
Size: 552 1089 923 3074
Fragments arranged by size:
3074 1089 923 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 3665 3682 3835 4020 4465 325
Size: 1255 888 1197 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1197 888 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2362 3884 5596
Size: 274 80 33 51 1443 385 1522 1712
Cuts at: 5596 96
Size: 138
Fragments arranged by size:
1712 1522 1443 385 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 2911
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 2493 3767 2493
Size: 1274 4364
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI