Restriction map for
pFN21AB9271
(Circular) (Six-base) MAPSORT of: pFN21AB9271.seq Check: 1169 from: 1 to: 6469
pFN21AB9271 6469 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2234 2246 2234
Size: 12 6457
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3812 1937
Size: 1875 4594
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3703 4532 5916 6184 1632
Size: 2071 829 1384 268 1917
Fragments arranged by size:
2071 1917 1384 829 268
AflII C'TTAA_G
Cuts at: 820 1017 2497 820
Size: 197 1480 4792
Fragments arranged by size:
4792 1480 197
AflIII A'CryG_T
Cuts at: 1203 5368 6345 1203
Size: 4165 977 1327
Fragments arranged by size:
4165 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 3342 1166
Size: 2176 4293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6469
AlwNI CAG_nnn'CTG
Cuts at: 2203 4212 5784 2203
Size: 2009 1572 2888
Fragments arranged by size:
2888 2009 1572
ApaI G_GGCC'C
Cuts at: 2644 2644
Size: 6469
ApaLI G'TGCA_C
Cuts at: 4526 5682 4526
Size: 1156 5313
ApoI r'AATT_y
Cuts at: 1493 2391 3565 3778 4004 4156 6265 1493
Size: 898 1174 213 226 152 2109 1697
Fragments arranged by size:
2109 1697 1174 898 226 213 152
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2922 3794 1870
Size: 73 979 872 4545
Fragments arranged by size:
4545 979 872 73
BamHI G'GATC_C
Cuts at: 3585 3799 4166 3585
Size: 214 367 5888
Fragments arranged by size:
5888 367 214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2076 3790 5245 618
Size: 325 217 627 224 65 1714 1455 1842
Fragments arranged by size:
1842 1714 1455 627 325 224 217 65
BanII G_rGCy'C
Cuts at: 1391 2644 3788 1391
Size: 1253 1144 4072
Fragments arranged by size:
4072 1253 1144
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 6469
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 928
Size: 971 147 5351
Fragments arranged by size:
5351 971 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4660 4694 1903
Size: 34 2723 34 3678
Fragments arranged by size:
3678 2723 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5080 136
Size: 107 122 71 631 605 337 3071 1525
Fragments arranged by size:
3071 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 3886 3886
Size: 6469
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2644 4530 5686 1790
Size: 289 565 1886 1156 2573
Fragments arranged by size:
2573 1886 1156 565 289
BmgBI CAC'GTC
Cuts at: 1318 2044 1318
Size: 726 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 2763 4200
Size: 416 53 83 399 24 739 607 1437
Cuts at: 4200 5158 442
Size: 958 1753
Fragments arranged by size:
1753 1437 958 739 607 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2566 2326
Size: 240 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2082 2141 2691 3195 3531 3615 3699 5129
Size: 843 59 550 504 336 84 84 1430
Cuts at: 5129 1239
Size: 2579
Fragments arranged by size:
2579 1430 843 550 504 336 84 84
59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2326 2428 2538 4594 5459 5757 2326
Size: 102 110 2056 865 298 3038
Fragments arranged by size:
3038 2056 865 298 110 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 2146 5132 882
Size: 617 618 29 2986 2219
Fragments arranged by size:
2986 2219 618 617 29
BsaAI yAC'GTr
Cuts at: 493 1204 4260 493
Size: 711 3056 2702
Fragments arranged by size:
3056 2702 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 4276 4658 5293
Size: 53 83 186 936 479 2264 382 635
Cuts at: 5293 275
Size: 1451
Fragments arranged by size:
2264 1451 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2837 3257 4902 5300 5574 5721 6132 2837
Size: 420 1645 398 274 147 411 3174
Fragments arranged by size:
3174 1645 420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2957 2987 1178
Size: 30 165 30 1554 30 4660
Fragments arranged by size:
4660 1554 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2965 1186
Size: 1779 4690
BseYI C'CCAG_C
Cuts at: 5672 6168 5672
Size: 496 5973
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3382 1163
Size: 573 1646 4250
Fragments arranged by size:
4250 1646 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2823 4530 4615 5686 2823
Size: 1707 85 1071 3606
Fragments arranged by size:
3606 1707 1071 85
BsmI GAATG_Cn'
Cuts at: 2876 3617 3701 3980 4073 2876
Size: 741 84 279 93 5272
Fragments arranged by size:
5272 741 279 93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2644 2823 3788 4530 4615 5686
Size: 399 289 565 179 965 742 85 1071
Cuts at: 5686 1391
Size: 2174
Fragments arranged by size:
2174 1071 965 742 565 399 289 179
85
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2135 2234 2246 2793 3568 3824 844
Size: 1291 99 12 547 775 256 3489
Fragments arranged by size:
3489 1291 775 547 256 99 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2889 3057 4356 1394
Size: 429 1066 168 1299 3507
Fragments arranged by size:
3507 1299 1066 429 168
BsrDI GCAATG_nn'
Cuts at: 65 1248 2698 3706 4964 5138 65
Size: 1183 1450 1008 1258 174 1396
Fragments arranged by size:
1450 1396 1258 1183 1008 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 5113 5300 1700
Size: 110 204 3099 187 2869
Fragments arranged by size:
3099 2869 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 4529 5541 4529
Size: 1012 5457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6469
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 2336 3585 3799 4166 4551 4568 6020 6106
Size: 364 1249 214 367 385 17 1452 86
Cuts at: 6106 6118 6464 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1249 385 367 364 346 214
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4978
Fragments arranged by size:
4978 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3206 487
Size: 683 56 1980 3750
Fragments arranged by size:
3750 1980 683 56
BtsI GCAGTG_nn'
Cuts at: 3370 3985 4779 4799 3370
Size: 615 794 20 5040
Fragments arranged by size:
5040 794 615 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2680 2715 2848 2883 2932 2967 3268
Size: 35 2116 35 133 35 49 35 301
Cuts at: 3268 3303 3688 3723 529
Size: 35 385 35 3275
Fragments arranged by size:
3275 2116 385 301 133 49 35 35
35 35 35 35
DraI TTT'AAA
Cuts at: 3773 3773
Size: 6469
DraIII CAC_nnn'GTG
Cuts at: 3079 3079
Size: 6469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3916 4805 6434
Size: 54 1425 50 275 18 2086 889 1629
Cuts at: 6434 8
Size: 43
Fragments arranged by size:
2086 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 2896 3316 3484 4399 1353
Size: 857 686 420 168 915 3423
Fragments arranged by size:
3423 915 857 686 420 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5056 5430 5576 387
Size: 1064 138 3467 374 146 1280
Fragments arranged by size:
3467 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2141 2691 3195 3531 3615 3699
Size: 393 450 59 550 504 336 84 84
Cuts at: 3699 3703 4532 5129 5916 6184 1239
Size: 4 829 597 787 268 1524
Fragments arranged by size:
1524 829 787 597 550 504 450 393
336 268 84 84 59 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 2105
Size: 6469
EcoO109I rG'GnC_Cy
Cuts at: 2097 2641 3249 3417 2097
Size: 544 608 168 5149
Fragments arranged by size:
5149 608 544 168
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
FspI TGC'GCA
Cuts at: 2242 4975 2242
Size: 2733 3736
HaeII r_GCGC'y
Cuts at: 1678 2015 5616 1678
Size: 337 3601 2531
Fragments arranged by size:
3601 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2905 2937 5110 5142 5184
Size: 32 135 32 1333 32 2173 32 42
Cuts at: 5184 5216 1373
Size: 32 2626
Fragments arranged by size:
2626 2173 1333 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3813 4059 669
Size: 988 281 1875 246 3079
Fragments arranged by size:
3079 1875 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2958 748
Size: 2210 4259
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3262 5583 5767 1305
Size: 1957 2321 184 2007
Fragments arranged by size:
2321 2007 1957 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4647
Fragments arranged by size:
4647 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2544 2770 3106 3342 4427 4786 4945
Size: 648 1378 226 336 236 1085 359 159
Cuts at: 4945 518
Size: 2042
Fragments arranged by size:
2042 1378 1085 648 359 336 236 226
159
MspA1I CmG'CkG
Cuts at: 1606 2290 3885 4562 5710 5955 1606
Size: 684 1595 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 684 677 245
NaeI GCC'GGC
Cuts at: 2016 2016
Size: 6469
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 6469
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4978
Fragments arranged by size:
4978 939 552
NdeI CA'TA_TG
Cuts at: 387 2739 2991 3327 387
Size: 2352 252 336 3529
Fragments arranged by size:
3529 2352 336 252
NgoMIV G'CCGG_C
Cuts at: 2014 2014
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 2607 3827 5372 1132
Size: 97 1378 1220 1545 2229
Fragments arranged by size:
2229 1545 1378 1220 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6469
PfoI T'CCnGG_A
Cuts at: 2316 2316
Size: 6469
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 3249 3417 2097
Size: 1152 168 5149
Fragments arranged by size:
5149 1152 168
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 2460 4039 2460
Size: 1579 4890
PspOMI G'GGCC_C
Cuts at: 2640 2640
Size: 6469
PstI C_TGCA'G
Cuts at: 830 2132 3821 830
Size: 1302 1689 3478
Fragments arranged by size:
3478 1689 1302
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 2290 2290
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SapI GCTCTTCn'nnn_
Cuts at: 2896 3316 3484 2896
Size: 420 168 5881
Fragments arranged by size:
5881 420 168
SbfI CC_TGCA'GG
Cuts at: 2132 3821 2132
Size: 1689 4780
ScaI AGT'ACT
Cuts at: 4717 6429 4717
Size: 1712 4757
SfcI C'TryA_G
Cuts at: 826 1045 2128 2968 3472 3817 4952 5633 5824
Size: 219 1083 840 504 345 1135 681 191
Cuts at: 5824 826
Size: 1471
Fragments arranged by size:
1471 1135 1083 840 681 504 345 219
191
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 6469
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 2014 1700
Size: 314 6155
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2341 2407 2497 2553 2922 4573
Size: 197 926 398 66 90 56 369 1651
Cuts at: 4573 5474 5736 820
Size: 901 262 1553
Fragments arranged by size:
1651 1553 926 901 398 369 262 197
90 66 56
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 4393 5
Size: 47 4341 2081
Fragments arranged by size:
4341 2081 47
StuI AGG'CCT
Cuts at: 1517 2223 3044 3212 3296 1517
Size: 706 821 168 84 4690
Fragments arranged by size:
4690 821 706 168 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3438 3908 513
Size: 552 939 1434 470 3074
Fragments arranged by size:
3074 1434 939 552 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2897 4496 4513 4666 4851 5296 325
Size: 1255 1317 1599 17 153 185 445 1498
Fragments arranged by size:
1599 1498 1317 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4715 6427 96
Size: 274 80 33 51 1443 2738 1712 138
Fragments arranged by size:
2738 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3409 3441 6429 6461 3409
Size: 32 2988 32 3417
Fragments arranged by size:
3417 2988 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 2922 1943
Size: 979 5490
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 2792 3632 4598 2792
Size: 840 966 4663
Fragments arranged by size:
4663 966 840
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI
XcmI