Restriction map for
pFN21AB9261
(Circular) (Six-base) MAPSORT of: pFN21AB9261.seq Check: 5599 from: 1 to: 5317
pFN21AB9261 5317 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3127 278
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 1937
Size: 723 4594
Acc65I G'GTAC_C
Cuts at: 2638 2638
Size: 5317
AclI AA'CG_TT
Cuts at: 1563 3444 3817 1563
Size: 1881 373 3063
Fragments arranged by size:
3063 1881 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2428 3380 4764 5032 1632
Size: 796 952 1384 268 1917
Fragments arranged by size:
1917 1384 952 796 268
AfeI AGC'GCT
Cuts at: 2028 2588 2028
Size: 560 4757
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5120
AflIII A'CryG_T
Cuts at: 1203 4216 5193 1203
Size: 3013 977 1327
Fragments arranged by size:
3013 1327 977
AgeI A'CCGG_T
Cuts at: 4148 4148
Size: 5317
AhdI GACnn_n'nnGTC
Cuts at: 4046 4046
Size: 5317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5317
AlwNI CAG_nnn'CTG
Cuts at: 3060 4632 3060
Size: 1572 3745
ApaI G_GGCC'C
Cuts at: 2014 2014
Size: 5317
ApaLI G'TGCA_C
Cuts at: 3374 4530 3374
Size: 1156 4161
ApoI r'AATT_y
Cuts at: 1493 2626 2852 3004 5113 1493
Size: 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3871 160
Size: 872 2839 1606
Fragments arranged by size:
2839 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
AvaI C'yCGr_G
Cuts at: 1870 1943 2394 2642 1870
Size: 73 451 248 4545
Fragments arranged by size:
4545 451 248 73
BamHI G'GATC_C
Cuts at: 2647 3014 2647
Size: 367 4950
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2072 2417 2463 2638
Size: 325 217 627 246 39 345 46 175
Cuts at: 2638 4093 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 345 325 246 217 175
46 39
BanII G_rGCy'C
Cuts at: 1391 2014 2636 1391
Size: 623 622 4072
Fragments arranged by size:
4072 623 622
BbeI G_GCGC'C
Cuts at: 2037 2076 2037
Size: 39 5278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 928
Size: 971 423 3923
Fragments arranged by size:
3923 971 423
BbvCI CC'TCA_GC
Cuts at: 2579 2579
Size: 5317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3508 3542 1903
Size: 34 1571 34 3678
Fragments arranged by size:
3678 1571 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3209 4419 3209
Size: 1210 4107
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3928 136
Size: 107 122 71 631 605 337 1919 1525
Fragments arranged by size:
1919 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5312 5312
Size: 5317
BlpI GC'TnA_GC
Cuts at: 2734 2734
Size: 5317
Bme1580I G_kGCm'C
Cuts at: 1790 2014 3378 4534 1790
Size: 224 1364 1156 2573
Fragments arranged by size:
2573 1364 1156 224
BmgBI CAC'GTC
Cuts at: 1318 2113 2176 1318
Size: 795 63 4459
Fragments arranged by size:
4459 795 63
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3048 4006 442
Size: 416 53 83 399 24 1631 958 1753
Fragments arranged by size:
1753 1631 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 3977 1239
Size: 1007 1731 2579
Fragments arranged by size:
2579 1731 1007
Bpu10I CC'TnA_GC
Cuts at: 2579 2579
Size: 5317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3442 4307 4605 3442
Size: 865 298 4154
Fragments arranged by size:
4154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3980 882
Size: 617 2481 2219
Fragments arranged by size:
2481 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3108 493
Size: 711 1904 2702
Fragments arranged by size:
2702 1904 711
BsaBI GATnn'nnATC
Cuts at: 5311 5311
Size: 5317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2073 3124 3506
Size: 53 83 186 936 501 39 1051 382
Cuts at: 3506 4141 275
Size: 635 1451
Fragments arranged by size:
1451 1051 936 635 501 382 186 83
53 39
BsaWI w'CCGG_w
Cuts at: 3750 4148 4422 4569 4980 3750
Size: 398 274 147 411 4087
Fragments arranged by size:
4087 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 2219 2249 1178
Size: 30 165 30 696 30 90 30 4246
Fragments arranged by size:
4246 696 165 90 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5317
BseYI C'CCAG_C
Cuts at: 2029 4520 5016 2029
Size: 2491 496 2330
Fragments arranged by size:
2491 2330 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 1163
Size: 573 777 3967
Fragments arranged by size:
3967 777 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2767 3528 4556 1540
Size: 275 186 766 761 1028 2301
Fragments arranged by size:
2301 1028 766 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3378 3463 4534 3378
Size: 85 1071 4161
Fragments arranged by size:
4161 1071 85
BsmI GAATG_Cn'
Cuts at: 2828 2921 2828
Size: 93 5224
BsmBI CGTCTCn'nnnn_
Cuts at: 2119 2119
Size: 5317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2636 3378 3463 4534 1391
Size: 399 224 622 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 622 399 224 85
BspEI T'CCGG_A
Cuts at: 4980 4980
Size: 5317
BspHI T'CATG_A
Cuts at: 3206 4936 3206
Size: 1730 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2493 2672 844
Size: 1649 179 3489
Fragments arranged by size:
3489 1649 179
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 3204 1394
Size: 429 194 1187 3507
Fragments arranged by size:
3507 1187 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 3812 3986 65
Size: 1183 2564 174 1396
Fragments arranged by size:
2564 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2087 3961 4148 1700
Size: 110 277 1874 187 2869
Fragments arranged by size:
2869 1874 277 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5317
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5317
BssSI C'ACGA_G
Cuts at: 3377 4389 3377
Size: 1012 4305
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5317
BstBI TT'CG_AA
Cuts at: 4121 4121
Size: 5317
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5317
BstYI r'GATC_y
Cuts at: 1972 2229 2647 3014 3399 3416 4868 4954 4966
Size: 257 418 367 385 17 1452 86 12
Cuts at: 4966 5312 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 418 385 367 346 257 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2197 2406 513
Size: 552 939 193 209 3424
Fragments arranged by size:
3424 939 552 209 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4578
Fragments arranged by size:
4578 683 56
BtsI GCAGTG_nn'
Cuts at: 2833 3627 3647 2833
Size: 794 20 4503
Fragments arranged by size:
4503 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5282
DraI TTT'AAA
Cuts at: 2621 2621
Size: 5317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2111 4324 809
Size: 507 795 2213 1802
Fragments arranged by size:
2213 1802 795 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2119 2764
Size: 54 1425 50 275 18 259 30 645
Cuts at: 2764 3653 5282 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 645 275 259 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2764 1537
Size: 275 952 4090
Fragments arranged by size:
4090 952 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3247 1353
Size: 1894 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3904 4278 4424 387
Size: 1064 138 2315 374 146 1280
Fragments arranged by size:
2315 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2428 3380 3977 4764 5032 1239
Size: 393 614 182 952 597 787 268 1524
Fragments arranged by size:
1524 952 787 614 597 393 268 182
EcoO109I rG'GnC_Cy
Cuts at: 2010 2011 2010
Size: 1 5316
EcoRI G'AATT_C
Cuts at: 2626 3004 2626
Size: 378 4939
FspI TGC'GCA
Cuts at: 3823 3823
Size: 5317
HaeII r_GCGC'y
Cuts at: 1678 2030 2037 2076 2590 4464 1678
Size: 352 7 39 514 1874 2531
Fragments arranged by size:
2531 1874 514 352 39 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3958 3990 4032 4064 1373
Size: 32 135 32 2386 32 42 32 2626
Fragments arranged by size:
2626 2386 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2661 2907 669
Size: 988 281 723 246 3079
Fragments arranged by size:
3079 988 723 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5317
HpaI GTT'AAC
Cuts at: 2907 2907
Size: 5317
KasI G'GCGC_C
Cuts at: 2033 2072 2033
Size: 39 5278
KpnI G_GTAC'C
Cuts at: 2642 2642
Size: 5317
MfeI C'AATT_G
Cuts at: 2916 2916
Size: 5317
MluI A'CGCG_T
Cuts at: 5193 5193
Size: 5317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4431 4615 1305
Size: 3126 184 2007
Fragments arranged by size:
3126 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3495
Fragments arranged by size:
3495 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3275 3634 3793 518
Size: 648 2109 359 159 2042
Fragments arranged by size:
2109 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2199 2205 2733 3410 4558 4803 1606
Size: 593 6 528 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 593 528 245 6
NaeI GCC'GGC
Cuts at: 2089 2089
Size: 5317
NarI GG'CG_CC
Cuts at: 2034 2073 2034
Size: 39 5278
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3826
Fragments arranged by size:
3826 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5317
NgoMIV G'CCGG_C
Cuts at: 2087 2087
Size: 5317
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5317
NotI GC'GGCC_GC
Cuts at: 2764 2764
Size: 5317
NspI r_CATG'y
Cuts at: 1132 1229 2675 4220 1132
Size: 97 1446 1545 2229
Fragments arranged by size:
2229 1545 1446 97
PasI CC'CwG_GG
Cuts at: 1348 2100 1348
Size: 752 4565
PciI A'CATG_T
Cuts at: 4216 4216
Size: 5317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 1108
Size: 939 4378
PmeI GTTT'AAAC
Cuts at: 2621 2621
Size: 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 4899 4931 3386
Size: 32 1481 32 3772
Fragments arranged by size:
3772 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5317
PsiI TTA'TAA
Cuts at: 2887 2887
Size: 5317
PspOMI G'GGCC_C
Cuts at: 2010 2010
Size: 5317
PstI C_TGCA'G
Cuts at: 830 2612 2669 830
Size: 1782 57 3478
Fragments arranged by size:
3478 1782 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5317
SacII CC_GC'GG
Cuts at: 2200 2200
Size: 5317
SalI G'TCGA_C
Cuts at: 1936 2659 1936
Size: 723 4594
SbfI CC_TGCA'GG
Cuts at: 2669 2669
Size: 5317
ScaI AGT'ACT
Cuts at: 3565 5277 3565
Size: 1712 3605
SfcI C'TryA_G
Cuts at: 826 1045 2608 2665 3800 4481 4672 826
Size: 219 1563 57 1135 681 191 1471
Fragments arranged by size:
1563 1471 1135 681 219 191 57
SfoI GGC'GCC
Cuts at: 2035 2074 2035
Size: 39 5278
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5317
SmaI CCC'GGG
Cuts at: 1872 2396 2644 1872
Size: 524 248 4545
Fragments arranged by size:
4545 524 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3421 4322 4584 820
Size: 197 926 1478 901 262 1553
Fragments arranged by size:
1553 1478 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3108 493
Size: 2615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5317
SphI G_CATG'C
Cuts at: 1132 2675 1132
Size: 1543 3774
SspI AAT'ATT
Cuts at: 5 52 3241 5
Size: 47 3189 2081
Fragments arranged by size:
3189 2081 47
StuI AGG'CCT
Cuts at: 1517 2480 2606 1517
Size: 963 126 4228
Fragments arranged by size:
4228 963 126
StyI C'CwwG_G
Cuts at: 513 1065 2004 2756 513
Size: 552 939 752 3074
Fragments arranged by size:
3074 939 752 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 3344 3361 3514 3699 4144 325
Size: 1255 702 1062 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1062 702 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3563 5275 96
Size: 274 80 33 51 1443 1586 1712 138
Fragments arranged by size:
1712 1586 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 5277 5309 2219
Size: 32 3026 32 2227
Fragments arranged by size:
3026 2227 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5317
XbaI T'CTAG_A
Cuts at: 2653 2653
Size: 5317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2344
Size: 5317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5317
XmaI C'CCGG_G
Cuts at: 1870 2394 2642 1870
Size: 524 248 4545
Fragments arranged by size:
4545 524 248
XmnI GAAnn'nnTTC
Cuts at: 3446 3446
Size: 5317
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3125 276
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI