Restriction map for
pFN21AB9239
(Circular) (Six-base) MAPSORT of: pFN21AB9239.seq Check: 5400 from: 1 to: 8995
pFN21AB9239 8995 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2285 2285
Size: 8995
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2271 5313 5874 6805
Size: 53 83 186 936 735 3042 561 931
Cuts at: 6805 278
Size: 2468
Fragments arranged by size:
3042 2468 936 931 735 561 186 83
53
AccI GT'mk_AC
Cuts at: 1937 3252 5874 6338 1937
Size: 1315 2622 464 4594
Fragments arranged by size:
4594 2622 1315 464
Acc65I G'GTAC_C
Cuts at: 6316 6316
Size: 8995
AclI AA'CG_TT
Cuts at: 1563 2678 7122 7495 1563
Size: 1115 4444 373 3063
Fragments arranged by size:
4444 3063 1115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2361 2710 4604 4652 5813 5824 6203
Size: 596 133 349 1894 48 1161 11 379
Cuts at: 6203 7058 8442 8710 1632
Size: 855 1384 268 1917
Fragments arranged by size:
1917 1894 1384 1161 855 596 379 349
268 133 48 11
AflII C'TTAA_G
Cuts at: 820 1017 2423 820
Size: 197 1406 7392
Fragments arranged by size:
7392 1406 197
AflIII A'CryG_T
Cuts at: 1203 7894 8871 1203
Size: 6691 977 1327
Fragments arranged by size:
6691 1327 977
AgeI A'CCGG_T
Cuts at: 7826 7826
Size: 8995
AhdI GACnn_n'nnGTC
Cuts at: 2771 7724 2771
Size: 4953 4042
AleI CACnn'nnGTG
Cuts at: 1166 4899 1166
Size: 3733 5262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3474 1184
Size: 2290 6705
AlwNI CAG_nnn'CTG
Cuts at: 3394 3560 4054 5218 5728 5791 6738 8310 3394
Size: 166 494 1164 510 63 947 1572 4079
Fragments arranged by size:
4079 1572 1164 947 510 494 166 63
ApaLI G'TGCA_C
Cuts at: 7052 8208 7052
Size: 1156 7839
ApoI r'AATT_y
Cuts at: 1493 2129 2357 2370 2522 2708 2888 3290 6304
Size: 636 228 13 152 186 180 402 3014
Cuts at: 6304 6530 6682 8791 1493
Size: 226 152 2109 1697
Fragments arranged by size:
3014 2109 1697 636 402 228 226 186
180 152 152 13
AseI AT'TA_AT
Cuts at: 160 1032 7549 160
Size: 872 6517 1606
Fragments arranged by size:
6517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8995
AvaI C'yCGr_G
Cuts at: 1870 1943 3211 4090 5280 6241 6291 6320 1870
Size: 73 1268 879 1190 961 50 29 4545
Fragments arranged by size:
4545 1268 1190 961 879 73 50 29
BamHI G'GATC_C
Cuts at: 2779 4094 5469 6325 6692 2779
Size: 1315 1375 856 367 5082
Fragments arranged by size:
5082 1375 1315 856 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5221 5985 6125 6257 6316
Size: 325 217 627 3434 764 140 132 59
Cuts at: 6316 7771 618
Size: 1455 1842
Fragments arranged by size:
3434 1842 1455 764 627 325 217 140
132 59
BanII G_rGCy'C
Cuts at: 1391 3101 4539 5694 5720 5748 6052 6314 1391
Size: 1710 1438 1155 26 28 304 262 4072
Fragments arranged by size:
4072 1710 1438 1155 304 262 28 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2770 3030 4167 928
Size: 971 871 260 1137 5756
Fragments arranged by size:
5756 1137 971 871 260
BbvCI CC'TCA_GC
Cuts at: 3695 4139 3695
Size: 444 8551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3599 3633 4279 4313 7186 7220 1903
Size: 34 1662 34 646 34 2873 34 3678
Fragments arranged by size:
3678 2873 1662 646 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2701 4000 6298 6887 8097 2701
Size: 1299 2298 589 1210 3599
Fragments arranged by size:
3599 2298 1299 1210 589
BclI T'GATC_A
Cuts at: 1548 5322 1548
Size: 3774 5221
BfrBI ATG'CAT
Cuts at: 5909 5909
Size: 8995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7606 136
Size: 107 122 71 631 605 5934 1525
Fragments arranged by size:
5934 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3946 8990 3946
Size: 5044 3951
BlpI GC'TnA_GC
Cuts at: 5040 6412 5040
Size: 1372 7623
Bme1580I G_kGCm'C
Cuts at: 1790 2743 5226 5403 5785 6128 7056 8212 1790
Size: 953 2483 177 382 343 928 1156 2573
Fragments arranged by size:
2573 2483 1156 953 928 382 343 177
BmgBI CAC'GTC
Cuts at: 1318 3277 3382 3877 4503 4987 5098 5236 1318
Size: 1959 105 495 626 484 111 138 5077
Fragments arranged by size:
5077 1959 626 495 484 138 111 105
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3265 4592 6726
Size: 416 53 83 399 24 1848 1327 2134
Cuts at: 6726 7684 442
Size: 958 1753
Fragments arranged by size:
2134 1848 1753 1327 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 8995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2529 3357 4171 4346 4719 4951 5627
Size: 860 430 828 814 175 373 232 676
Cuts at: 5627 7655 1239
Size: 2028 2579
Fragments arranged by size:
2579 2028 860 828 814 676 430 373
232 175
Bpu10I CC'TnA_GC
Cuts at: 3695 4139 5288 5714 3695
Size: 444 1149 426 6976
Fragments arranged by size:
6976 1149 444 426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2612 4058 4109 7120 7985 8283 2612
Size: 1446 51 3011 865 298 3324
Fragments arranged by size:
3324 3011 1446 865 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 5153 7658 882
Size: 617 2490 1164 2505 2219
Fragments arranged by size:
2505 2490 2219 1164 617
BsaAI yAC'GTr
Cuts at: 493 1204 3355 3976 6786 493
Size: 711 2151 621 2810 2702
Fragments arranged by size:
2810 2702 2151 711 621
BsaBI GATnn'nnATC
Cuts at: 2477 5069 8989 2477
Size: 2592 3920 2483
Fragments arranged by size:
3920 2592 2483
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 3542 4159 5310
Size: 53 83 186 936 735 1274 617 1151
Cuts at: 5310 5871 6802 7184 7819 275
Size: 561 931 382 635 1451
Fragments arranged by size:
1451 1274 1151 936 931 735 635 617
561 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3882 7428 7826 8100 8247 8658 3882
Size: 3546 398 274 147 411 4219
Fragments arranged by size:
4219 3546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2381 2411 3894 3924 1178
Size: 30 165 30 978 30 1483 30 6249
Fragments arranged by size:
6249 1483 978 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3683 4156 4294 5033 5255 5375 5915 1186
Size: 2497 473 138 739 222 120 540 4266
Fragments arranged by size:
4266 2497 739 540 473 222 138 120
BseYI C'CCAG_C
Cuts at: 4802 5314 5721 6068 6151 8198 8694 4802
Size: 512 407 347 83 2047 496 5103
Fragments arranged by size:
5103 2047 512 496 407 347 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 2378 3418 3434 3830 5009 5110
Size: 573 489 153 1040 16 396 1179 101
Cuts at: 5110 5159 5324 5671 1163
Size: 49 165 347 4487
Fragments arranged by size:
4487 1179 1040 573 489 396 347 165
153 101 49 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4368 4694 6445 7206 8234 1540
Size: 275 186 2367 326 1751 761 1028 2301
Fragments arranged by size:
2367 2301 1751 1028 761 326 275 186
BsiHKAI G_wGCw'C
Cuts at: 3975 5739 6119 7056 7141 8212 3975
Size: 1764 380 937 85 1071 4758
Fragments arranged by size:
4758 1764 1071 937 380 85
BsmI GAATG_Cn'
Cuts at: 6506 6599 6506
Size: 93 8902
BsmBI CGTCTCn'nnnn_
Cuts at: 3533 5603 3533
Size: 2070 6925
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2743 3101 3975 4539 5226 5403 5694
Size: 399 953 358 874 564 687 177 291
Cuts at: 5694 5720 5739 5748 5785 6052 6119 6128 6314
Size: 26 19 9 37 267 67 9 186
Cuts at: 6314 7056 7141 8212 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 953 874 742 687 564 399
358 291 267 186 177 85 67 37
26 19 9 9
BspEI T'CCGG_A
Cuts at: 8658 8658
Size: 8995
BspHI T'CATG_A
Cuts at: 4677 5751 6884 8614 4677
Size: 1074 1133 1730 5058
Fragments arranged by size:
5058 1730 1133 1074
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2285 2400 3971 4062 4182 5346 6350 844
Size: 1441 115 1571 91 120 1164 1004 3489
Fragments arranged by size:
3489 1571 1441 1164 1004 120 115 91
BsrBI CCG'CTC
Cuts at: 1394 1823 2191 5346 6882 1394
Size: 429 368 3155 1536 3507
Fragments arranged by size:
3507 3155 1536 429 368
BsrDI GCAATG_nn'
Cuts at: 65 1248 3156 3639 5034 6226 7490 7664 65
Size: 1183 1908 483 1395 1192 1264 174 1396
Fragments arranged by size:
1908 1396 1395 1264 1192 1183 483 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2108 2160 3503 4693 5463 7639
Size: 110 238 60 52 1343 1190 770 2176
Cuts at: 7639 7826 1700
Size: 187 2869
Fragments arranged by size:
2869 2176 1343 1190 770 238 187 110
60 52
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8995
BssSI C'ACGA_G
Cuts at: 2243 3968 6020 7055 8067 2243
Size: 1725 2052 1035 1012 3171
Fragments arranged by size:
3171 2052 1725 1035 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5853 794
Size: 5059 3936
BstBI TT'CG_AA
Cuts at: 4000 4837 7799 4000
Size: 837 2962 5196
Fragments arranged by size:
5196 2962 837
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8995
BstXI CCAn_nnnn'nTGG
Cuts at: 5818 5818
Size: 8995
BstYI r'GATC_y
Cuts at: 1972 2214 2779 3946 4094 4545 5253 5469 6325
Size: 242 565 1167 148 451 708 216 856
Cuts at: 6325 6692 7077 7094 8546 8632 8644 8990 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1167 856 708 565 451 385
367 346 242 216 148 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5027 5027
Size: 8995
BtgI C'CryG_G
Cuts at: 513 1065 3924 5154 5165 5199 5265 5301 5403
Size: 552 2859 1230 11 34 66 36 102
Cuts at: 5403 5539 5572 5599 5765 5817 513
Size: 136 33 27 166 52 3691
Fragments arranged by size:
3691 2859 1230 552 166 136 102 66
52 36 34 33 27 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3018 487
Size: 683 56 1792 6464
Fragments arranged by size:
6464 1792 683 56
BtsI GCAGTG_nn'
Cuts at: 2071 4357 6511 7305 7325 2071
Size: 2286 2154 794 20 3741
Fragments arranged by size:
3741 2286 2154 794 20
ClaI AT'CG_AT
Cuts at: 1554 3219 1554
Size: 1665 7330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8960
DraI TTT'AAA
Cuts at: 2527 6299 2527
Size: 3772 5223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8002 809
Size: 507 6686 1802
Fragments arranged by size:
6686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3271 3370 3483
Size: 54 1425 50 275 18 1441 99 113
Cuts at: 3483 3594 3855 4084 4365 4659 5010 5532 5575
Size: 111 261 229 281 294 351 522 43
Cuts at: 5575 5673 6223 6442 7331 8960 8
Size: 98 550 219 889 1629 43
Fragments arranged by size:
1629 1441 1425 889 550 522 351 294
281 275 261 229 219 113 111 99
98 54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4365 6442 1537
Size: 275 2553 2077 4090
Fragments arranged by size:
4090 2553 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 2783 4028 4527 6925 1353
Size: 984 446 1245 499 2398 3423
Fragments arranged by size:
3423 2398 1245 984 499 446
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5495 7582 7956 8102 387
Size: 1064 138 3906 2087 374 146 1280
Fragments arranged by size:
3906 2087 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2228 2361 2529 2710 3357 4171
Size: 393 467 129 133 168 181 647 814
Cuts at: 4171 4346 4604 4652 4719 4951 5627 5813 5824
Size: 175 258 48 67 232 676 186 11
Cuts at: 5824 6203 7058 7655 8442 8710 1239
Size: 379 855 597 787 268 1524
Fragments arranged by size:
1524 855 814 787 676 647 597 467
393 379 268 258 232 186 181 175
168 133 129 67 48 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 3562 5975 2243
Size: 1319 2413 5263
Fragments arranged by size:
5263 2413 1319
EcoO109I rG'GnC_Cy
Cuts at: 2643 5023 5284 2643
Size: 2380 261 6354
Fragments arranged by size:
6354 2380 261
EcoRI G'AATT_C
Cuts at: 6304 6682 6304
Size: 378 8617
EcoRV GAT'ATC
Cuts at: 2940 2940
Size: 8995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5854 5854
Size: 8995
FspI TGC'GCA
Cuts at: 5393 7501 5393
Size: 2108 6887
FspAI rTGC'GCAy
Cuts at: 5393 5393
Size: 8995
HaeII r_GCGC'y
Cuts at: 1678 3920 5174 5218 8142 1678
Size: 2242 1254 44 2924 2531
Fragments arranged by size:
2924 2531 2242 1254 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2757 2789 4011 4043 4361
Size: 32 135 32 1185 32 1222 32 318
Cuts at: 4361 4393 4487 4519 5861 5893 7636 7668 7710
Size: 32 94 32 1342 32 1743 32 42
Cuts at: 7710 7742 1373
Size: 32 2626
Fragments arranged by size:
2626 1743 1342 1222 1185 318 135 94
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2572 2701 3121 5569 6339 6585
Size: 988 281 634 129 420 2448 770 246
Cuts at: 6585 669
Size: 3079
Fragments arranged by size:
3079 2448 988 770 634 420 281 246
129
HindIII A'AGCT_T
Cuts at: 748 5836 748
Size: 5088 3907
HpaI GTT'AAC
Cuts at: 3121 6585 3121
Size: 3464 5531
KpnI G_GTAC'C
Cuts at: 6320 6320
Size: 8995
MfeI C'AATT_G
Cuts at: 6594 6594
Size: 8995
MluI A'CGCG_T
Cuts at: 8871 8871
Size: 8995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3132 3846 4063 8109 8293 1305
Size: 1827 714 217 4046 184 2007
Fragments arranged by size:
4046 2007 1827 714 217 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3273 3372 3485 4086 4661
Size: 54 1425 343 1441 99 113 601 575
Cuts at: 4661 5012 5534 5675 6225 10
Size: 351 522 141 550 2780
Fragments arranged by size:
2780 1441 1425 601 575 550 522 351
343 141 113 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2226 4899 6131 6953 7312 7471
Size: 648 843 217 2673 1232 822 359 159
Cuts at: 7471 518
Size: 2042
Fragments arranged by size:
2673 2042 1232 843 822 648 359 217
159
MspA1I CmG'CkG
Cuts at: 1606 2696 4552 4957 4999 5118 5295 5601 5728
Size: 1090 1856 405 42 119 177 306 127
Cuts at: 5728 6255 6411 7088 8236 8481 1606
Size: 527 156 677 1148 245 2120
Fragments arranged by size:
2120 1856 1148 1090 677 527 405 306
245 177 156 127 119 42
NaeI GCC'GGC
Cuts at: 2162 3505 2162
Size: 1343 7652
NcoI C'CATG_G
Cuts at: 513 1065 5265 5301 5817 513
Size: 552 4200 36 516 3691
Fragments arranged by size:
4200 3691 552 516 36
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8995
NgoMIV G'CCGG_C
Cuts at: 2160 3503 2160
Size: 1343 7652
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8995
NotI GC'GGCC_GC
Cuts at: 6442 6442
Size: 8995
NsiI A_TGCA'T
Cuts at: 5911 5911
Size: 8995
NspI r_CATG'y
Cuts at: 1132 1229 3759 3775 6353 7898 1132
Size: 97 2530 16 2578 1545 2229
Fragments arranged by size:
2578 2530 2229 1545 97 16
PasI CC'CwG_GG
Cuts at: 1348 5971 1348
Size: 4623 4372
PciI A'CATG_T
Cuts at: 7894 7894
Size: 8995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2657 5218 1108
Size: 1549 2561 4885
Fragments arranged by size:
4885 2561 1549
PfoI T'CCnGG_A
Cuts at: 3225 3423 4097 4928 5106 3225
Size: 198 674 831 178 7114
Fragments arranged by size:
7114 831 674 198 178
PmeI GTTT'AAAC
Cuts at: 6299 6299
Size: 8995
PmlI CAC'GTG
Cuts at: 3355 3355
Size: 8995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7064 7096 8577 8609 7064
Size: 32 1481 32 7450
Fragments arranged by size:
7450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5023 5284 5023
Size: 261 8734
PshAI GACnn'nnGTC
Cuts at: 1613 2267 1613
Size: 654 8341
PsiI TTA'TAA
Cuts at: 6565 6565
Size: 8995
PspXI vC'TCGA_Gb
Cuts at: 6241 6241
Size: 8995
PstI C_TGCA'G
Cuts at: 830 3087 4059 4971 5253 6347 830
Size: 2257 972 912 282 1094 3478
Fragments arranged by size:
3478 2257 1094 972 912 282
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8995
PvuII CAG'CTG
Cuts at: 4552 4957 4999 5728 4552
Size: 405 42 729 7819
Fragments arranged by size:
7819 729 405 42
SacII CC_GC'GG
Cuts at: 5602 5602
Size: 8995
SalI G'TCGA_C
Cuts at: 1936 6337 1936
Size: 4401 4594
SapI GCTCTTCn'nnn_
Cuts at: 4527 4527
Size: 8995
SbfI CC_TGCA'GG
Cuts at: 6347 6347
Size: 8995
ScaI AGT'ACT
Cuts at: 7243 8955 7243
Size: 1712 7283
SfcI C'TryA_G
Cuts at: 826 1045 3083 3188 4055 4967 5249 6343 7478
Size: 219 2038 105 867 912 282 1094 1135
Cuts at: 7478 8159 8350 826
Size: 681 191 1471
Fragments arranged by size:
2038 1471 1135 1094 912 867 681 282
219 191 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8995
SgrAI Cr'CCGG_yG
Cuts at: 1700 2048 1700
Size: 348 8647
SmaI CCC'GGG
Cuts at: 1872 5282 6293 6322 1872
Size: 3410 1011 29 4545
Fragments arranged by size:
4545 3410 1011 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 2591 3211 4073 4124 6241
Size: 197 926 480 168 620 862 51 2117
Cuts at: 6241 7099 8000 8262 820
Size: 858 901 262 1553
Fragments arranged by size:
2117 1553 926 901 862 858 620 480
262 197 168 51
SnaBI TAC'GTA
Cuts at: 493 6786 493
Size: 6293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8995
SphI G_CATG'C
Cuts at: 1132 6353 1132
Size: 5221 3774
SspI AAT'ATT
Cuts at: 5 52 3178 6919 5
Size: 47 3126 3741 2081
Fragments arranged by size:
3741 3126 2081 47
StuI AGG'CCT
Cuts at: 1517 3081 6009 6233 1517
Size: 1564 2928 224 4279
Fragments arranged by size:
4279 2928 1564 224
StyI C'CwwG_G
Cuts at: 513 1065 3700 4383 5265 5301 5817 6054 6434
Size: 552 2635 683 882 36 516 237 380
Cuts at: 6434 513
Size: 3074
Fragments arranged by size:
3074 2635 882 683 552 516 380 237
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4679 4864 5503 7022 7039 7192 7377
Size: 1255 3099 185 639 1519 17 153 185
Cuts at: 7377 7822 325
Size: 445 1498
Fragments arranged by size:
3099 1519 1498 1255 639 445 185 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6030 7241 8953
Size: 274 80 33 51 1443 4053 1211 1712
Cuts at: 8953 96
Size: 138
Fragments arranged by size:
4053 1712 1443 1211 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4079 4111 4443 4475 5633 5665 8955 8987 4079
Size: 32 332 32 1158 32 3290 32 4087
Fragments arranged by size:
4087 3290 1158 332 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2152 3071 3666 3878 1316
Size: 836 919 595 212 6433
Fragments arranged by size:
6433 919 836 595 212
XbaI T'CTAG_A
Cuts at: 4528 6331 4528
Size: 1803 7192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4321 5893 4321
Size: 1572 7423
XhoI C'TCGA_G
Cuts at: 1943 3211 6241 1943
Size: 1268 3030 4697
Fragments arranged by size:
4697 3030 1268
XmaI C'CCGG_G
Cuts at: 1870 5280 6291 6320 1870
Size: 3410 1011 29 4545
Fragments arranged by size:
4545 3410 1011 29
XmnI GAAnn'nnTTC
Cuts at: 5088 7124 5088
Size: 2036 6959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2269 5311 5872 6803
Size: 53 83 186 936 735 3042 561 931
Cuts at: 6803 276
Size: 2468
Fragments arranged by size:
3042 2468 936 931 735 561 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I DraIII EcoICRI FseI KasI NarI NruI PacI
PspOMI PsrI RsrII SacI SanDI SexAI SfiI SfoI
SrfI SwaI