Restriction map for
pFN21AB9177
(Circular) (Six-base) MAPSORT of: pFN21AB9177.seq Check: 7624 from: 1 to: 8119
pFN21AB9177 8119 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2391 4039 4328 2391
Size: 1648 289 6182
Fragments arranged by size:
6182 1648 289
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5929 278
Size: 53 83 186 936 4393 2468
Fragments arranged by size:
4393 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 5462 1937
Size: 1548 1977 4594
Fragments arranged by size:
4594 1977 1548
Acc65I G'GTAC_C
Cuts at: 5440 5440
Size: 8119
AclI AA'CG_TT
Cuts at: 1563 6246 6619 1563
Size: 4683 373 3063
Fragments arranged by size:
4683 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4284 4613 6182 7566 7834 1632
Size: 2652 329 1569 1384 268 1917
Fragments arranged by size:
2652 1917 1569 1384 329 268
AfeI AGC'GCT
Cuts at: 2117 2117
Size: 8119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7922
AflIII A'CryG_T
Cuts at: 1203 2443 3910 5361 7018 7995 1203
Size: 1240 1467 1451 1657 977 1327
Fragments arranged by size:
1657 1467 1451 1327 1240 977
AgeI A'CCGG_T
Cuts at: 2988 6950 2988
Size: 3962 4157
AhdI GACnn_n'nnGTC
Cuts at: 6848 6848
Size: 8119
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4241 1184
Size: 3057 5062
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2519 2551 3254 3286 3537 3569 3782 3814 4964
Size: 32 703 32 251 32 213 32 1150
Cuts at: 4964 4996 2519
Size: 32 5642
Fragments arranged by size:
5642 1150 703 251 213 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 4285 4371 4873 4912 5862 7434 4285
Size: 86 502 39 950 1572 4970
Fragments arranged by size:
4970 1572 950 502 86 39
ApaI G_GGCC'C
Cuts at: 2180 3893 4241 2180
Size: 1713 348 6058
Fragments arranged by size:
6058 1713 348
ApaLI G'TGCA_C
Cuts at: 2360 2447 3029 6176 7332 2360
Size: 87 582 3147 1156 3147
Fragments arranged by size:
3147 3147 1156 582 87
ApoI r'AATT_y
Cuts at: 1493 5428 5654 5806 7915 1493
Size: 3935 226 152 2109 1697
Fragments arranged by size:
3935 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6673 160
Size: 872 5641 1606
Fragments arranged by size:
5641 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8119
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 3719 4178 4411 4481 4830 5007
Size: 73 282 1494 459 233 70 349 177
Cuts at: 5007 5444 1870
Size: 437 4545
Fragments arranged by size:
4545 1494 459 437 349 282 233 177
73 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2191 2224 2191
Size: 33 8086
BamHI G'GATC_C
Cuts at: 2489 3051 5449 5816 2489
Size: 562 2398 367 4792
Fragments arranged by size:
4792 2398 562 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2759 3241 3715 3842
Size: 325 217 627 230 742 482 474 127
Cuts at: 3842 5440 6895 618
Size: 1598 1455 1842
Fragments arranged by size:
1842 1598 1455 742 627 482 474 325
230 217 127
BanII G_rGCy'C
Cuts at: 1391 2165 2180 2380 3893 4179 4241 4307 4488
Size: 774 15 200 1513 286 62 66 181
Cuts at: 4488 5190 5438 1391
Size: 702 248 4072
Fragments arranged by size:
4072 1513 774 702 286 248 200 181
66 62 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 3227 5039 928
Size: 971 403 925 1812 4008
Fragments arranged by size:
4008 1812 971 925 403
BbvCI CC'TCA_GC
Cuts at: 4233 5409 4233
Size: 1176 6943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2940 2974 6310 6344 1903
Size: 34 1003 34 3336 34 3678
Fragments arranged by size:
3678 3336 1003 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 6011 7221 3539
Size: 2472 1210 4437
Fragments arranged by size:
4437 2472 1210
BclI T'GATC_A
Cuts at: 1548 4575 1548
Size: 3027 5092
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2613 2748 5348
Size: 107 122 71 631 605 941 135 2600
Cuts at: 5348 6730 136
Size: 1382 1525
Fragments arranged by size:
2600 1525 1382 941 631 605 135 122
107 71
BglII A'GATC_T
Cuts at: 8114 8114
Size: 8119
BlpI GC'TnA_GC
Cuts at: 3566 5536 3566
Size: 1970 6149
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2180 2364 2451 2690 2822 2926 3033
Size: 232 158 184 87 239 132 104 107
Cuts at: 3033 3246 3342 3351 3720 3893 4241 6180 7336
Size: 213 96 9 369 173 348 1939 1156
Cuts at: 7336 1790
Size: 2573
Fragments arranged by size:
2573 1939 1156 369 348 239 232 213
184 173 158 132 107 104 96 87
9
BmgBI CAC'GTC
Cuts at: 1318 2470 4087 1318
Size: 1152 1617 5350
Fragments arranged by size:
5350 1617 1152
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 3921 4235
Size: 416 53 83 399 24 2081 423 314
Cuts at: 4235 4493 5850 6808 442
Size: 258 1357 958 1753
Fragments arranged by size:
2081 1753 1357 958 423 416 399 314
258 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8119
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3811 4648 3811
Size: 837 7282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2394 2914 2939 3104 3921 4207 6779
Size: 974 181 520 25 165 817 286 2572
Cuts at: 6779 1239
Size: 2579
Fragments arranged by size:
2579 2572 974 817 520 286 181 165
25
Bpu10I CC'TnA_GC
Cuts at: 3633 4233 4441 4490 4856 5409 3633
Size: 600 208 49 366 553 6343
Fragments arranged by size:
6343 600 553 366 208 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6244 7109 7407 6244
Size: 865 298 6956
Fragments arranged by size:
6956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3403 3415 4062 4415 6782 882
Size: 617 1904 12 647 353 2367 2219
Fragments arranged by size:
2367 2219 1904 647 617 353 12
BsaAI yAC'GTr
Cuts at: 493 1204 2359 2444 3913 5910 493
Size: 711 1155 85 1469 1997 2702
Fragments arranged by size:
2702 1997 1469 1155 711 85
BsaBI GATnn'nnATC
Cuts at: 8113 8113
Size: 8119
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2307 5044 5926
Size: 53 83 186 936 636 138 2737 882
Cuts at: 5926 6308 6943 275
Size: 382 635 1451
Fragments arranged by size:
2737 1451 936 882 636 635 382 186
138 83 53
BsaWI w'CCGG_w
Cuts at: 2988 3048 6552 6950 7224 7371 7782 2988
Size: 60 3504 398 274 147 411 3325
Fragments arranged by size:
3504 3325 411 398 274 147 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3351 3381 4964 4994 5004
Size: 30 165 30 1948 30 1583 30 10
Cuts at: 5004 5034 1178
Size: 30 4263
Fragments arranged by size:
4263 1948 1583 165 30 30 30 30
30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3600 4115 4433 5030 5426 1186
Size: 2414 515 318 597 396 3879
Fragments arranged by size:
3879 2414 597 515 396 318
BseYI C'CCAG_C
Cuts at: 2112 2330 2654 2661 4055 4819 7322 7818 2112
Size: 218 324 7 1394 764 2503 496 2413
Fragments arranged by size:
2503 2413 1394 764 496 324 218 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2424 2579 3141 3342 3434 3581 3862
Size: 573 688 155 562 201 92 147 281
Cuts at: 3862 3884 3891 4067 4083 1163
Size: 22 7 176 16 5199
Fragments arranged by size:
5199 688 573 562 281 201 176 155
147 92 22 16 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2206 5569 6330 7358 1540
Size: 275 186 205 3363 761 1028 2301
Fragments arranged by size:
3363 2301 1028 761 275 205 186
BsiHKAI G_wGCw'C
Cuts at: 2364 2451 3033 3189 5190 6180 6265 7336 2364
Size: 87 582 156 2001 990 85 1071 3147
Fragments arranged by size:
3147 2001 1071 990 582 156 87 85
BsmI GAATG_Cn'
Cuts at: 4167 5630 5723 4167
Size: 1463 93 6563
Fragments arranged by size:
6563 1463 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3092 3799 4378 5051 3092
Size: 707 579 673 6160
Fragments arranged by size:
6160 707 673 579
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2165 2180 2364 2380 2451 2690
Size: 399 232 143 15 184 16 71 239
Cuts at: 2690 2822 2926 3033 3189 3246 3342 3351 3720
Size: 132 104 107 156 57 96 9 369
Cuts at: 3720 3893 4179 4241 4307 4488 5190 5438 6180
Size: 173 286 62 66 181 702 248 742
Cuts at: 6180 6265 7336 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 399 369 286 248
239 232 184 181 173 156 143 132
107 104 96 85 71 66 62 57
16 15 9
BspEI T'CCGG_A
Cuts at: 7782 7782
Size: 8119
BspHI T'CATG_A
Cuts at: 3615 6008 7738 3615
Size: 2393 1730 3996
Fragments arranged by size:
3996 2393 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2391 4039 4328 4785 5474 844
Size: 1259 288 1648 289 457 689 3489
Fragments arranged by size:
3489 1648 1259 689 457 289 288
BsrBI CCG'CTC
Cuts at: 1394 1823 2574 3781 6006 1394
Size: 429 751 1207 2225 3507
Fragments arranged by size:
3507 2225 1207 751 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6614 6788 65
Size: 1183 5366 174 1396
Fragments arranged by size:
5366 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2988 3312 3456 3570 3684 6763 6950
Size: 110 1178 324 144 114 114 3079 187
Cuts at: 6950 1700
Size: 2869
Fragments arranged by size:
3079 2869 1178 324 187 144 114 114
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8119
BssHII G'CGCG_C
Cuts at: 1925 2246 1925
Size: 321 7798
BssSI C'ACGA_G
Cuts at: 2300 6179 7191 2300
Size: 3879 1012 3228
Fragments arranged by size:
3879 3228 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3028 4371 4873 794
Size: 2234 1343 502 4040
Fragments arranged by size:
4040 2234 1343 502
BstBI TT'CG_AA
Cuts at: 6923 6923
Size: 8119
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8119
BstXI CCAn_nnnn'nTGG
Cuts at: 2596 2611 3496 2596
Size: 15 885 7219
Fragments arranged by size:
7219 885 15
BstYI r'GATC_y
Cuts at: 1972 2489 2885 3051 3060 3981 5449 5816 6201
Size: 517 396 166 9 921 1468 367 385
Cuts at: 6201 6218 7670 7756 7768 8114 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1468 1452 921 517 396 385 367
346 166 86 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 3530 4098 3530
Size: 568 7551
BtgI C'CryG_G
Cuts at: 513 1065 2004 3474 3650 4120 513
Size: 552 939 1470 176 470 4512
Fragments arranged by size:
4512 1470 939 552 470 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7380
Fragments arranged by size:
7380 683 56
BtsI GCAGTG_nn'
Cuts at: 2822 3813 3874 5635 6429 6449 2822
Size: 991 61 1761 794 20 4492
Fragments arranged by size:
4492 1761 991 794 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8084
DraI TTT'AAA
Cuts at: 5423 5423
Size: 8119
DraIII CAC_nnn'GTG
Cuts at: 2410 2410
Size: 8119
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2134 7126 809
Size: 507 818 4992 1802
Fragments arranged by size:
4992 1802 818 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 3301 3572
Size: 54 1425 50 275 18 258 1213 271
Cuts at: 3572 4335 4466 4555 4893 5064 5566 6455 8084
Size: 763 131 89 338 171 502 889 1629
Cuts at: 8084 8
Size: 43
Fragments arranged by size:
1629 1425 1213 889 763 502 338 275
271 258 171 131 89 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5566 1537
Size: 275 3754 4090
Fragments arranged by size:
4090 3754 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2474 4154 4197 4260 6049 1353
Size: 695 426 1680 43 63 1789 3423
Fragments arranged by size:
3423 1789 1680 695 426 63 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3434 6706 7080 7226 387
Size: 1064 138 1845 3272 374 146 1280
Fragments arranged by size:
3272 1845 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5188 5188
Size: 8119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2394 2914 2939 3104 3921 4207
Size: 393 581 181 520 25 165 817 286
Cuts at: 4207 4284 4613 6182 6779 7566 7834 1239
Size: 77 329 1569 597 787 268 1524
Fragments arranged by size:
1569 1524 817 787 597 581 520 393
329 286 268 181 165 77 25
EcoO109I rG'GnC_Cy
Cuts at: 2176 2842 3594 3602 3964 4237 4270 4446 4531
Size: 666 752 8 362 273 33 176 85
Cuts at: 4531 4719 4804 4939 2176
Size: 188 85 135 5356
Fragments arranged by size:
5356 752 666 362 273 188 176 135
85 85 33 8
EcoRI G'AATT_C
Cuts at: 5428 5806 5428
Size: 378 7741
FspI TGC'GCA
Cuts at: 2216 6625 2216
Size: 4409 3710
FspAI rTGC'GCAy
Cuts at: 2216 2216
Size: 8119
HaeII r_GCGC'y
Cuts at: 1678 2119 2622 2662 3319 7266 1678
Size: 441 503 40 657 3947 2531
Fragments arranged by size:
3947 2531 657 503 441 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5029 5061 5099 5131 6760
Size: 32 135 32 3457 32 38 32 1629
Cuts at: 6760 6792 6834 6866 1373
Size: 32 42 32 2626
Fragments arranged by size:
3457 2626 1629 135 42 38 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 5463 5709 669
Size: 988 281 1548 1977 246 3079
Fragments arranged by size:
3079 1977 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2916 748
Size: 2168 5951
HpaI GTT'AAC
Cuts at: 5709 5709
Size: 8119
KpnI G_GTAC'C
Cuts at: 5444 5444
Size: 8119
MfeI C'AATT_G
Cuts at: 5718 5718
Size: 8119
MluI A'CGCG_T
Cuts at: 7995 7995
Size: 8119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3636 4945 7233 7417 1305
Size: 2331 1309 2288 184 2007
Fragments arranged by size:
2331 2288 2007 1309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 5066 10
Size: 54 1425 343 258 2976 3063
Fragments arranged by size:
3063 2976 1425 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2706 2721 3494 3614 4613 6077
Size: 648 1191 349 15 773 120 999 1464
Cuts at: 6077 6436 6595 518
Size: 359 159 2042
Fragments arranged by size:
2042 1464 1191 999 773 648 359 349
159 120 15
MspA1I CmG'CkG
Cuts at: 1606 2429 2766 3125 3230 3406 3565 3856 3988
Size: 823 337 359 105 176 159 291 132
Cuts at: 3988 4262 4285 4870 5408 5535 6212 7360 7605
Size: 274 23 585 538 127 677 1148 245
Cuts at: 7605 1606
Size: 2120
Fragments arranged by size:
2120 1148 823 677 585 538 359 337
291 274 245 176 159 132 127 105
23
NaeI GCC'GGC
Cuts at: 3314 3572 3314
Size: 258 7861
NcoI C'CATG_G
Cuts at: 513 1065 2004 3474 513
Size: 552 939 1470 5158
Fragments arranged by size:
5158 1470 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8119
NgoMIV G'CCGG_C
Cuts at: 3312 3570 3312
Size: 258 7861
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8119
NotI GC'GGCC_GC
Cuts at: 5566 5566
Size: 8119
NspI r_CATG'y
Cuts at: 1132 1229 3671 4159 4906 5335 5365 5477 7022
Size: 97 2442 488 747 429 30 112 1545
Cuts at: 7022 1132
Size: 2229
Fragments arranged by size:
2442 2229 1545 747 488 429 112 97
30
PasI CC'CwG_GG
Cuts at: 1348 2611 4213 5206 1348
Size: 1263 1602 993 4261
Fragments arranged by size:
4261 1602 1263 993
PciI A'CATG_T
Cuts at: 5361 7018 5361
Size: 1657 6462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3394 3480 1108
Size: 902 1384 86 5747
Fragments arranged by size:
5747 1384 902 86
PmeI GTTT'AAAC
Cuts at: 5423 5423
Size: 8119
PmlI CAC'GTG
Cuts at: 2444 3913 2444
Size: 1469 6650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4910 4942 4964 4996 6188 6220 7701 7733 4910
Size: 32 22 32 1192 32 1481 32 5296
Fragments arranged by size:
5296 1481 1192 32 32 32 32 22
PpuMI rG'GwC_Cy
Cuts at: 4446 4531 4804 4939 4446
Size: 85 273 135 7626
Fragments arranged by size:
7626 273 135 85
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8119
PsiI TTA'TAA
Cuts at: 5689 5689
Size: 8119
PspOMI G'GGCC_C
Cuts at: 2176 3889 4237 2176
Size: 1713 348 6058
Fragments arranged by size:
6058 1713 348
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4721 4753 5369 5401 4721
Size: 32 616 32 7439
Fragments arranged by size:
7439 616 32 32
PstI C_TGCA'G
Cuts at: 830 2547 2783 3130 4230 4434 4479 5471 830
Size: 1717 236 347 1100 204 45 992 3478
Fragments arranged by size:
3478 1717 1100 992 347 236 204 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8119
PvuII CAG'CTG
Cuts at: 3125 3230 3565 3856 3988 4262 4285 4870 5408
Size: 105 335 291 132 274 23 585 538
Cuts at: 5408 3125
Size: 5836
Fragments arranged by size:
5836 585 538 335 291 274 132 105
23
SacI G_AGCT'C
Cuts at: 5190 5190
Size: 8119
SalI G'TCGA_C
Cuts at: 1936 3484 5461 1936
Size: 1548 1977 4594
Fragments arranged by size:
4594 1977 1548
SapI GCTCTTCn'nnn_
Cuts at: 2474 2474
Size: 8119
SbfI CC_TGCA'GG
Cuts at: 5471 5471
Size: 8119
ScaI AGT'ACT
Cuts at: 6367 8079 6367
Size: 1712 6407
SexAI A'CCwGG_T
Cuts at: 2262 2262
Size: 8119
SfcI C'TryA_G
Cuts at: 826 1045 2543 2581 2779 3126 4226 4430 4475
Size: 219 1498 38 198 347 1100 204 45
Cuts at: 4475 5083 5318 5467 6602 7283 7474 826
Size: 608 235 149 1135 681 191 1471
Fragments arranged by size:
1498 1471 1135 1100 681 608 347 235
219 204 198 191 149 45 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8119
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8119
SmaI CCC'GGG
Cuts at: 1872 2227 3721 5446 1872
Size: 355 1494 1725 4545
Fragments arranged by size:
4545 1725 1494 355
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4830 6223 7124 7386 820
Size: 197 926 2887 1393 901 262 1553
Fragments arranged by size:
2887 1553 1393 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5910 493
Size: 5417 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8119
SphI G_CATG'C
Cuts at: 1132 4159 4906 5335 5477 1132
Size: 3027 747 429 142 3774
Fragments arranged by size:
3774 3027 747 429 142
SrfI GCCC'GGGC
Cuts at: 2227 3721 2227
Size: 1494 6625
SspI AAT'ATT
Cuts at: 5 52 6043 5
Size: 47 5991 2081
Fragments arranged by size:
5991 2081 47
StuI AGG'CCT
Cuts at: 1517 2777 4096 5351 1517
Size: 1260 1319 1255 4285
Fragments arranged by size:
4285 1319 1260 1255
StyI C'CwwG_G
Cuts at: 513 1065 2004 2679 3198 3474 3711 5149 5558
Size: 552 939 675 519 276 237 1438 409
Cuts at: 5558 513
Size: 3074
Fragments arranged by size:
3074 1438 939 675 552 519 409 276
237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3006 3860 6146 6163 6316 6501 6946
Size: 1255 1426 854 2286 17 153 185 445
Cuts at: 6946 325
Size: 1498
Fragments arranged by size:
2286 1498 1426 1255 854 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2431 6365 8077
Size: 274 80 33 51 1443 454 3934 1712
Cuts at: 8077 96
Size: 138
Fragments arranged by size:
3934 1712 1443 454 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3103 3135 3351 3383 3613 3645 4661 4693 8079
Size: 32 216 32 230 32 1016 32 3386
Cuts at: 8079 8111 3103
Size: 32 3111
Fragments arranged by size:
3386 3111 1016 230 216 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2396 1316
Size: 1080 7039
XbaI T'CTAG_A
Cuts at: 5455 5455
Size: 8119
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 2969 3205 4036 4506 2044
Size: 925 236 831 470 5657
Fragments arranged by size:
5657 925 831 470 236
XhoI C'TCGA_G
Cuts at: 1943 4830 1943
Size: 2887 5232
XmaI C'CCGG_G
Cuts at: 1870 2225 3719 5444 1870
Size: 355 1494 1725 4545
Fragments arranged by size:
4545 1725 1494 355
XmnI GAAnn'nnTTC
Cuts at: 3137 4925 6248 3137
Size: 1788 1323 5008
Fragments arranged by size:
5008 1788 1323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5927 276
Size: 53 83 186 936 4393 2468
Fragments arranged by size:
4393 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoNI EcoRV
FalI FseI KasI NarI NruI NsiI PacI PfoI
PspXI RsrII SacII SanDI SfiI SfoI SwaI