Restriction map for
pFN21AB9171
(Circular) (Six-base) MAPSORT of: pFN21AB9171.seq Check: 240 from: 1 to: 6712
pFN21AB9171 6712 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2745 2863 3415 2745
Size: 118 552 6042
Fragments arranged by size:
6042 552 118
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3291 3936 4522 278
Size: 53 83 186 936 1755 645 586 2468
Fragments arranged by size:
2468 1755 936 645 586 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4055 1937
Size: 2118 4594
Acc65I G'GTAC_C
Cuts at: 4033 4033
Size: 6712
AclI AA'CG_TT
Cuts at: 1563 4839 5212 1563
Size: 3276 373 3063
Fragments arranged by size:
3276 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4775 6159 6427 1632
Size: 3143 1384 268 1917
Fragments arranged by size:
3143 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 5611 6588 1203
Size: 4408 977 1327
Fragments arranged by size:
4408 1327 977
AgeI A'CCGG_T
Cuts at: 3659 5543 3659
Size: 1884 4828
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6712
AlwNI CAG_nnn'CTG
Cuts at: 2260 2330 2869 2878 2965 3458 3520 3943 4455
Size: 70 539 9 87 493 62 423 512
Cuts at: 4455 6027 2260
Size: 1572 2945
Fragments arranged by size:
2945 1572 539 512 493 423 87 70
62 9
ApaI G_GGCC'C
Cuts at: 3767 3767
Size: 6712
ApaLI G'TGCA_C
Cuts at: 2192 2522 4769 5925 2192
Size: 330 2247 1156 2979
Fragments arranged by size:
2979 2247 1156 330
ApoI r'AATT_y
Cuts at: 1493 4021 4247 4399 6508 1493
Size: 2528 226 152 2109 1697
Fragments arranged by size:
2528 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2293 2399 3360 3870 4037 1870
Size: 73 123 227 106 961 510 167 4545
Fragments arranged by size:
4545 961 510 227 167 123 106 73
AvrII C'CTAG_G
Cuts at: 2236 3901 2236
Size: 1665 5047
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2281 2314 2281
Size: 33 6679
BamHI G'GATC_C
Cuts at: 2499 2538 3861 4042 4409 2499
Size: 39 1323 181 367 4802
Fragments arranged by size:
4802 1323 367 181 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2403 2586 3004 3889 4033
Size: 325 217 627 616 183 418 885 144
Cuts at: 4033 5488 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 885 627 616 418 325 217
183 144
BanII G_rGCy'C
Cuts at: 1391 3110 3194 3514 3767 4031 1391
Size: 1719 84 320 253 264 4072
Fragments arranged by size:
4072 1719 320 264 253 84
BbeI G_GCGC'C
Cuts at: 3008 3008
Size: 6712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5741
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4903 4937 1903
Size: 34 2966 34 3678
Fragments arranged by size:
3678 2966 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2221 4604 5814 2221
Size: 2383 1210 3119
Fragments arranged by size:
3119 2383 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6712
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 2327 2401
Size: 107 122 71 631 605 493 162 74
Cuts at: 2401 3762 5323 136
Size: 1361 1561 1525
Fragments arranged by size:
1561 1525 1361 631 605 493 162 122
107 74 71
BglII A'GATC_T
Cuts at: 6707 6707
Size: 6712
BlpI GC'TnA_GC
Cuts at: 4129 4129
Size: 6712
Bme1580I G_kGCm'C
Cuts at: 1790 2196 2406 2526 3022 3043 3767 4773 5929
Size: 406 210 120 496 21 724 1006 1156
Cuts at: 5929 1790
Size: 2573
Fragments arranged by size:
2573 1156 1006 724 496 406 210 120
21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6712
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2524 4443 5401
Size: 416 53 83 399 24 1107 1919 958
Cuts at: 5401 442
Size: 1753
Fragments arranged by size:
1919 1753 1107 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3857 1056
Size: 2801 3911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3856 3856
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2382 2927 2945 3059 3435 3540 3851 3986
Size: 1143 545 18 114 376 105 311 135
Cuts at: 3986 5372 1239
Size: 1386 2579
Fragments arranged by size:
2579 1386 1143 545 376 311 135 114
105 18
Bpu10I CC'TnA_GC
Cuts at: 2088 2769 3671 2088
Size: 681 902 5129
Fragments arranged by size:
5129 902 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4837 5702 6000 4837
Size: 865 298 5549
Fragments arranged by size:
5549 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 2788 5375 882
Size: 617 720 569 2587 2219
Fragments arranged by size:
2587 2219 720 617 569
BsaAI yAC'GTr
Cuts at: 493 1204 2719 4503 493
Size: 711 1515 1784 2702
Fragments arranged by size:
2702 1784 1515 711
BsaBI GATnn'nnATC
Cuts at: 6706 6706
Size: 6712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2277 3005 3288 3933
Size: 53 83 186 936 744 728 283 645
Cuts at: 3933 4519 4901 5536 275
Size: 586 382 635 1451
Fragments arranged by size:
1451 936 744 728 645 635 586 382
283 186 83 53
BsaWI w'CCGG_w
Cuts at: 2264 3659 5145 5543 5817 5964 6375 2264
Size: 1395 1486 398 274 147 411 2601
Fragments arranged by size:
2601 1486 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2930 2960 3959 3989 1178
Size: 30 165 30 1527 30 999 30 3901
Fragments arranged by size:
3901 1527 999 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2261 3335 3380 3592 3848 1186
Size: 1006 69 1074 45 212 256 4050
Fragments arranged by size:
4050 1074 1006 256 212 69 45
BseYI C'CCAG_C
Cuts at: 2182 2564 2968 3110 3739 3849 5915 6411 2182
Size: 382 404 142 629 110 2066 496 2483
Fragments arranged by size:
2483 2066 629 496 404 382 142 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2451 2717 3168 3363 3759 1163
Size: 573 715 266 451 195 396 4116
Fragments arranged by size:
4116 715 573 451 396 266 195
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2890 4162 4923 5951 1540
Size: 275 186 889 1272 761 1028 2301
Fragments arranged by size:
2301 1272 1028 889 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2358 2526 3194 4773 4858 5929 2196
Size: 162 168 668 1579 85 1071 2979
Fragments arranged by size:
2979 1579 1071 668 168 162 85
BsmI GAATG_Cn'
Cuts at: 4223 4316 4223
Size: 93 6619
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2358 2406 2526 3022 3043 3110
Size: 399 406 162 48 120 496 21 67
Cuts at: 3110 3194 3514 3767 4031 4773 4858 5929 1391
Size: 84 320 253 264 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 496 406 399 320 264
253 162 120 85 84 67 48 21
BspEI T'CCGG_A
Cuts at: 2264 6375 2264
Size: 4111 2601
BspHI T'CATG_A
Cuts at: 4601 6331 4601
Size: 1730 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 2745 2863 3415 4067 844
Size: 1844 57 118 552 652 3489
Fragments arranged by size:
3489 1844 652 552 118 57
BsrBI CCG'CTC
Cuts at: 1394 1823 4599 1394
Size: 429 2776 3507
Fragments arranged by size:
3507 2776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3795 5207 5381 65
Size: 1183 2547 1412 174 1396
Fragments arranged by size:
2547 1412 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2882 3549 3659 5356 5543 1700
Size: 110 1072 667 110 1697 187 2869
Fragments arranged by size:
2869 1697 1072 667 187 110 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6712
BssHII G'CGCG_C
Cuts at: 1925 2310 1925
Size: 385 6327
BssSI C'ACGA_G
Cuts at: 3347 4772 5784 3347
Size: 1425 1012 4275
Fragments arranged by size:
4275 1425 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2740 794
Size: 1946 4766
BstBI TT'CG_AA
Cuts at: 5516 5516
Size: 6712
BstEII G'GTnAC_C
Cuts at: 1184 2972 1184
Size: 1788 4924
BstYI r'GATC_y
Cuts at: 1972 2499 2538 3861 4042 4409 4794 4811 6263
Size: 527 39 1323 181 367 385 17 1452
Cuts at: 6263 6349 6361 6707 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1323 527 385 367 346 181
86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2817 3314 2817
Size: 497 6215
BtgI C'CryG_G
Cuts at: 513 1065 2004 2407 2527 3505 3751 513
Size: 552 939 403 120 978 246 3474
Fragments arranged by size:
3474 978 939 552 403 246 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5973
Fragments arranged by size:
5973 683 56
BtsI GCAGTG_nn'
Cuts at: 2305 2649 3375 3644 3995 4228 5022 5042 2305
Size: 344 726 269 351 233 794 20 3975
Fragments arranged by size:
3975 794 726 351 344 269 233 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 4016 4016
Size: 6712
DraIII CAC_nnn'GTG
Cuts at: 4006 4006
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2567 5719 809
Size: 507 1251 3152 1802
Fragments arranged by size:
3152 1802 1251 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2319 2410
Size: 54 1425 50 275 18 210 279 91
Cuts at: 2410 2454 2530 2880 2887 3748 4159 5048 6677
Size: 44 76 350 7 861 411 889 1629
Cuts at: 6677 8
Size: 43
Fragments arranged by size:
1629 1425 889 861 411 350 279 275
210 91 76 54 50 44 43 18
7
EagI C'GGCC_G
Cuts at: 1537 1812 2887 4159 1537
Size: 275 1075 1272 4090
Fragments arranged by size:
4090 1272 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 3062 4642 1353
Size: 816 893 1580 3423
Fragments arranged by size:
3423 1580 893 816
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2951 5299 5673 5819 387
Size: 1064 138 1362 2348 374 146 1280
Fragments arranged by size:
2348 1362 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3192 3192
Size: 6712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2382 2927 2945 3059 3435 3540 3851
Size: 393 750 545 18 114 376 105 311
Cuts at: 3851 3986 4775 5372 6159 6427 1239
Size: 135 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 750 597 545 393 376
311 268 135 114 105 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 3317 3337 3830 3900 2240
Size: 1077 20 493 70 5052
Fragments arranged by size:
5052 1077 493 70 20
EcoO109I rG'GnC_Cy
Cuts at: 2233 2659 2812 3055 3370 3743 3763 3764 3905
Size: 426 153 243 315 373 20 1 141
Cuts at: 3905 3940 2233
Size: 35 5005
Fragments arranged by size:
5005 426 373 315 243 153 141 35
20 1
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
EcoRV GAT'ATC
Cuts at: 3265 3265
Size: 6712
FspI TGC'GCA
Cuts at: 5218 5218
Size: 6712
HaeII r_GCGC'y
Cuts at: 1678 2095 2261 2608 3008 3740 5859 1678
Size: 417 166 347 400 732 2119 2531
Fragments arranged by size:
2531 2119 732 417 400 347 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 2195 2227 2530
Size: 32 135 32 496 32 95 32 303
Cuts at: 2530 2562 2646 2678 5353 5385 5427 5459 1373
Size: 32 84 32 2675 32 42 32 2626
Fragments arranged by size:
2675 2626 496 303 135 95 84 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 4056 4302 669
Size: 988 281 739 1379 246 3079
Fragments arranged by size:
3079 1379 988 739 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6712
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KasI G'GCGC_C
Cuts at: 3004 3004
Size: 6712
KpnI G_GTAC'C
Cuts at: 4037 4037
Size: 6712
MfeI C'AATT_G
Cuts at: 4311 4311
Size: 6712
MluI A'CGCG_T
Cuts at: 6588 6588
Size: 6712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3319 5826 6010 1305
Size: 2014 2507 184 2007
Fragments arranged by size:
2507 2014 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2412 2456 2532 3750 10
Size: 54 1425 343 580 44 76 1218 2972
Fragments arranged by size:
2972 1425 1218 580 343 76 54 44
MslI CAynn'nnrTG
Cuts at: 518 1166 2592 4670 5029 5188 518
Size: 648 1426 2078 359 159 2042
Fragments arranged by size:
2078 2042 1426 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2341 2465 2866 3095 3176 3242 3544 3964
Size: 735 124 401 229 81 66 302 420
Cuts at: 3964 4128 4805 5953 6198 1606
Size: 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 735 677 420 401 302 245
229 164 124 81 66
NaeI GCC'GGC
Cuts at: 2884 2884
Size: 6712
NarI GG'CG_CC
Cuts at: 3005 3005
Size: 6712
NcoI C'CATG_G
Cuts at: 513 1065 2004 2407 2527 3751 513
Size: 552 939 403 120 1224 3474
Fragments arranged by size:
3474 1224 939 552 403 120
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6712
NgoMIV G'CCGG_C
Cuts at: 2882 2882
Size: 6712
NheI G'CTAG_C
Cuts at: 1052 3853 1052
Size: 2801 3911
NotI GC'GGCC_GC
Cuts at: 4159 4159
Size: 6712
NspI r_CATG'y
Cuts at: 1132 1229 2474 4070 5615 1132
Size: 97 1245 1596 1545 2229
Fragments arranged by size:
2229 1596 1545 1245 97
PasI CC'CwG_GG
Cuts at: 1348 3535 1348
Size: 2187 4525
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6712
PfoI T'CCnGG_A
Cuts at: 3198 3828 3198
Size: 630 6082
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2812 2233
Size: 579 6133
PshAI GACnn'nnGTC
Cuts at: 1613 2231 1613
Size: 618 6094
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PspOMI G'GGCC_C
Cuts at: 3763 3763
Size: 6712
PstI C_TGCA'G
Cuts at: 830 2256 2340 2874 3466 3762 4064 830
Size: 1426 84 534 592 296 302 3478
Fragments arranged by size:
3478 1426 592 534 302 296 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 2341 2465 2866 3176 3544 3964 2341
Size: 124 401 310 368 420 5089
Fragments arranged by size:
5089 420 401 368 310 124
SacI G_AGCT'C
Cuts at: 3194 3194
Size: 6712
SalI G'TCGA_C
Cuts at: 1936 4054 1936
Size: 2118 4594
SanDI GG'GwC_CC
Cuts at: 2812 2812
Size: 6712
SapI GCTCTTCn'nnn_
Cuts at: 3062 3062
Size: 6712
SbfI CC_TGCA'GG
Cuts at: 3762 4064 3762
Size: 302 6410
ScaI AGT'ACT
Cuts at: 4960 6672 4960
Size: 1712 5000
SexAI A'CCwGG_T
Cuts at: 2691 3723 4002 2691
Size: 1032 279 5401
Fragments arranged by size:
5401 1032 279
SfcI C'TryA_G
Cuts at: 826 1045 2252 2336 2870 3462 3758 4060 5195
Size: 219 1207 84 534 592 296 302 1135
Cuts at: 5195 5876 6067 826
Size: 681 191 1471
Fragments arranged by size:
1471 1207 1135 681 592 534 302 296
219 191 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 3762 3762
Size: 6712
SfoI GGC'GCC
Cuts at: 3006 3006
Size: 6712
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6712
SmaI CCC'GGG
Cuts at: 1872 2401 3362 3872 4039 1872
Size: 529 961 510 167 4545
Fragments arranged by size:
4545 961 529 510 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4816 5717 5979 820
Size: 197 926 2873 901 262 1553
Fragments arranged by size:
2873 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4503 493
Size: 4010 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 4070 1132
Size: 2938 3774
SspI AAT'ATT
Cuts at: 5 52 4636 5
Size: 47 4584 2081
Fragments arranged by size:
4584 2081 47
StuI AGG'CCT
Cuts at: 1517 2168 2395 3136 3498 1517
Size: 651 227 741 362 4731
Fragments arranged by size:
4731 741 651 362 227
StyI C'CwwG_G
Cuts at: 513 1065 2004 2236 2407 2527 3751 3901 4151
Size: 552 939 232 171 120 1224 150 250
Cuts at: 4151 513
Size: 3074
Fragments arranged by size:
3074 1224 939 552 250 232 171 150
120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2960 4739 4756 4909 5094 5539 325
Size: 1255 1380 1779 17 153 185 445 1498
Fragments arranged by size:
1779 1498 1380 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2956 4958 6670
Size: 274 80 33 51 1443 979 2002 1712
Cuts at: 6670 96
Size: 138
Fragments arranged by size:
2002 1712 1443 979 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 2930 2962 3194 3216 3226 3248 3959
Size: 32 772 32 232 22 10 22 711
Cuts at: 3959 3991 6672 6704 2126
Size: 32 2681 32 2134
Fragments arranged by size:
2681 2134 772 711 232 32 32 32
32 22 22 10
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6712
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3478 3478
Size: 6712
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 2399 3360 3870 4037 1870
Size: 529 961 510 167 4545
Fragments arranged by size:
4545 961 529 510 167
XmnI GAAnn'nnTTC
Cuts at: 4841 4841
Size: 6712
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3289 3934 4520 276
Size: 53 83 186 936 1755 645 586 2468
Fragments arranged by size:
2468 1755 936 645 586 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbvCI BfrBI BsiWI BsmBI BstXI
BstZ17I FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SrfI SwaI