Restriction map for
pFN21AB9140
(Circular) (Six-base) MAPSORT of: pFN21AB9140.seq Check: 453 from: 1 to: 5833
pFN21AB9140 5833 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2266 820
Size: 197 1249 4387
Fragments arranged by size:
4387 1249 197
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 2963 3576 5148 2963
Size: 613 1572 3648
Fragments arranged by size:
3648 1572 613
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2612 3142 3368 3520 5629 1493
Size: 1119 530 226 152 2109 1697
Fragments arranged by size:
2109 1697 1119 530 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 3158 1870
Size: 73 190 1025 4545
Fragments arranged by size:
4545 1025 190 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 3154 4609 618
Size: 325 217 627 235 1132 1455 1842
Fragments arranged by size:
1842 1455 1132 627 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2041 2123 2766 3152 1391
Size: 650 82 643 386 4072
Fragments arranged by size:
4072 650 643 386 82
BbeI G_GCGC'C
Cuts at: 2026 2026
Size: 5833
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2839 3051 928
Size: 971 940 212 3710
Fragments arranged by size:
3710 971 940 212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2512 5828 2512
Size: 3316 2517
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2913 3894 5050 1790
Size: 1123 981 1156 2573
Fragments arranged by size:
2573 1156 1123 981
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2025 2922 3102 4493 1239
Size: 786 897 180 1391 2579
Fragments arranged by size:
2579 1391 897 786 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2151 4496 882
Size: 617 652 2345 2219
Fragments arranged by size:
2345 2219 652 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2023 3640 4022 4657
Size: 53 83 186 936 490 1617 382 635
Cuts at: 4657 275
Size: 1451
Fragments arranged by size:
1617 1451 936 635 490 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2500 1186
Size: 910 404 4519
Fragments arranged by size:
4519 910 404
BseYI C'CCAG_C
Cuts at: 2550 5036 5532 2550
Size: 2486 496 2851
Fragments arranged by size:
2851 2486 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3283 4044 5072 1540
Size: 275 186 33 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2041 2123 2766 3894 3979 5050 2041
Size: 82 643 1128 85 1071 2824
Fragments arranged by size:
2824 1128 1071 643 85 82
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2123 2766 2913 3152 3894 3979
Size: 399 251 82 643 147 239 742 85
Cuts at: 3979 5050 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 643 399 251 239 147
85 82
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 2092 2766 3722 5452 2092
Size: 674 956 1730 2473
Fragments arranged by size:
2473 1730 956 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 844
Size: 2344 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2512 2929 2959 3163 3530 3915 3932 5384
Size: 540 417 30 204 367 385 17 1452
Cuts at: 5384 5470 5482 5828 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 540 417 385 367 346 204
86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 2559 2559
Size: 5833
BtgI C'CryG_G
Cuts at: 513 1065 2076 2246 513
Size: 552 1011 170 4100
Fragments arranged by size:
4100 1011 552 170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 2260 3349 4143 4163 2260
Size: 1089 794 20 3930
Fragments arranged by size:
3930 1089 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 4169 5798
Size: 54 1425 50 275 18 1450 889 1629
Cuts at: 5798 8
Size: 43
Fragments arranged by size:
1629 1450 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2736 3013 3763 1353
Size: 756 627 277 750 3423
Fragments arranged by size:
3423 756 750 627 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2039 2121 2764 2039
Size: 82 643 5108
Fragments arranged by size:
5108 643 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2025 2922 3102 3896 4493 5280 5548
Size: 393 393 897 180 794 597 787 268
Cuts at: 5548 1239
Size: 1524
Fragments arranged by size:
1524 897 794 787 597 393 393 268
180
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2193 2193
Size: 5833
FspI TGC'GCA
Cuts at: 2480 4339 2480
Size: 1859 3974
HaeII r_GCGC'y
Cuts at: 1678 2026 4980 1678
Size: 348 2954 2531
Fragments arranged by size:
2954 2531 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2023 2055 2087 2119 2655
Size: 32 135 32 451 32 32 32 536
Cuts at: 2655 2687 4474 4506 4548 4580 1373
Size: 32 1787 32 42 32 2626
Fragments arranged by size:
2626 1787 536 451 135 42 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 2022 2022
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3116 4947 5131 1305
Size: 1811 1831 184 2007
Fragments arranged by size:
2007 1831 1811 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3791 4150 4309 518
Size: 648 2625 359 159 2042
Fragments arranged by size:
2625 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 3249 3926 5074 5319 1606
Size: 452 1191 677 1148 245 2120
Fragments arranged by size:
2120 1191 1148 677 452 245
NarI GG'CG_CC
Cuts at: 2023 2023
Size: 5833
NcoI C'CATG_G
Cuts at: 513 1065 2076 513
Size: 552 1011 4270
Fragments arranged by size:
4270 1011 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2892 3191 4736 1132
Size: 97 1663 299 1545 2229
Fragments arranged by size:
2229 1663 1545 299 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PspXI vC'TCGA_Gb
Cuts at: 2133 2133
Size: 5833
PstI C_TGCA'G
Cuts at: 830 3185 830
Size: 2355 3478
PvuI CG_AT'CG
Cuts at: 2001 2034 2001
Size: 33 5800
PvuII CAG'CTG
Cuts at: 2058 2058
Size: 5833
SacI G_AGCT'C
Cuts at: 2041 2123 2766 2041
Size: 82 643 5108
Fragments arranged by size:
5108 643 82
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SapI GCTCTTCn'nnn_
Cuts at: 2736 2736
Size: 5833
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2311 3181 4316 4997 5188 826
Size: 219 1266 870 1135 681 191 1471
Fragments arranged by size:
1471 1266 1135 870 681 219 191
SfoI GGC'GCC
Cuts at: 2024 2024
Size: 5833
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2133 2266 3937 4838 5100 820
Size: 197 926 190 133 1671 901 262 1553
Fragments arranged by size:
1671 1553 926 901 262 197 190 133
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3070 3757 5
Size: 47 3018 687 2081
Fragments arranged by size:
3018 2081 687 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2076 3272 513
Size: 552 1011 1196 3074
Fragments arranged by size:
3074 1196 1011 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3024 3860 3877 4030 4215 4660 325
Size: 1255 1444 836 17 153 185 445 1498
Fragments arranged by size:
1498 1444 1255 836 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4079 5791 96
Size: 274 80 33 51 1443 2102 1712 138
Fragments arranged by size:
2102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 2099 3039 1316
Size: 783 940 4110
Fragments arranged by size:
4110 940 783
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2083
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 2133 1943
Size: 190 5643
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3058 3962 3058
Size: 904 4929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI