Restriction map for
pFN21AB9139
(Circular) (Six-base) MAPSORT of: pFN21AB9139.seq Check: 2590 from: 1 to: 6166
pFN21AB9139 6166 bp
With 182 enzymes: SgfI *
November 15, 2013 16:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3976 278
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2157 2406 3509 1937
Size: 220 249 1103 4594
Fragments arranged by size:
4594 1103 249 220
Acc65I G'GTAC_C
Cuts at: 2616 3487 2616
Size: 871 5295
AclI AA'CG_TT
Cuts at: 1563 3465 4293 4666 1563
Size: 1902 828 373 3063
Fragments arranged by size:
3063 1902 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 2701 2834 3026 4229 5613 5881 1632
Size: 1010 59 133 192 1203 1384 268 1917
Fragments arranged by size:
1917 1384 1203 1010 268 192 133 59
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5969
AflIII A'CryG_T
Cuts at: 1203 2994 5065 6042 1203
Size: 1791 2071 977 1327
Fragments arranged by size:
2071 1791 1327 977
AgeI A'CCGG_T
Cuts at: 4997 4997
Size: 6166
AhdI GACnn_n'nnGTC
Cuts at: 2098 4895 2098
Size: 2797 3369
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6166
AlwNI CAG_nnn'CTG
Cuts at: 3909 5481 3909
Size: 1572 4594
ApaI G_GGCC'C
Cuts at: 3143 3143
Size: 6166
ApaLI G'TGCA_C
Cuts at: 4223 5379 4223
Size: 1156 5010
ApoI r'AATT_y
Cuts at: 1493 3475 3701 3853 5962 1493
Size: 1982 226 152 2109 1697
Fragments arranged by size:
2109 1982 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4720 160
Size: 872 3688 1606
Fragments arranged by size:
3688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 3491 1870
Size: 73 102 1446 4545
Fragments arranged by size:
4545 1446 102 73
BamHI G'GATC_C
Cuts at: 3496 3863 3496
Size: 367 5799
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2168 2616 3487 4942
Size: 325 217 627 269 112 448 871 1455
Cuts at: 4942 618
Size: 1842
Fragments arranged by size:
1842 1455 871 627 448 325 269 217
112
BanII G_rGCy'C
Cuts at: 1391 2194 3143 3485 1391
Size: 803 949 342 4072
Fragments arranged by size:
4072 949 803 342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2630 2798 2822 3052 3420 928
Size: 971 731 168 24 230 368 3674
Fragments arranged by size:
3674 971 731 368 230 168 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3358 3392 4357 4391 1903
Size: 34 50 34 1337 34 965 34 3678
Fragments arranged by size:
3678 1337 965 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4058 5268 4058
Size: 1210 4956
BclI T'GATC_A
Cuts at: 1548 2658 1548
Size: 1110 5056
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2095 3170 4777
Size: 107 122 71 631 605 423 1075 1607
Cuts at: 4777 136
Size: 1525
Fragments arranged by size:
1607 1525 1075 631 605 423 122 107
71
BglII A'GATC_T
Cuts at: 3096 6161 3096
Size: 3065 3101
BlpI GC'TnA_GC
Cuts at: 3583 3583
Size: 6166
Bme1580I G_kGCm'C
Cuts at: 1790 2061 3143 4227 5383 1790
Size: 271 1082 1084 1156 2573
Fragments arranged by size:
2573 1156 1084 1082 271
BmgBI CAC'GTC
Cuts at: 1318 2752 1318
Size: 1434 4732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3238 3410 3897
Size: 416 53 83 399 24 1821 172 487
Cuts at: 3897 4855 442
Size: 958 1753
Fragments arranged by size:
1821 1753 958 487 416 399 172 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2270 2398 2705 2717 2914 4826 1239
Size: 860 171 128 307 12 197 1912 2579
Fragments arranged by size:
2579 1912 860 307 197 171 128 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 4291 5156 5454 2819
Size: 1472 865 298 3531
Fragments arranged by size:
3531 1472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3323 4829 882
Size: 617 1824 1506 2219
Fragments arranged by size:
2219 1824 1506 617
BsaAI yAC'GTr
Cuts at: 493 1204 3957 493
Size: 711 2753 2702
Fragments arranged by size:
2753 2702 711
BsaBI GATnn'nnATC
Cuts at: 6160 6160
Size: 6166
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 3973 4355 4990
Size: 53 83 186 936 495 1945 382 635
Cuts at: 4990 275
Size: 1451
Fragments arranged by size:
1945 1451 936 635 495 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4599 4997 5271 5418 5829 4599
Size: 398 274 147 411 4936
Fragments arranged by size:
4936 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3320 3350 1178
Size: 30 165 30 1917 30 3994
Fragments arranged by size:
3994 1917 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 1186
Size: 910 5256
BseYI C'CCAG_C
Cuts at: 2485 2770 2901 3136 3421 5369 5865 2485
Size: 285 131 235 285 1948 496 2786
Fragments arranged by size:
2786 1948 496 285 285 235 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5593
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2455 3616 4377 5405 1540
Size: 275 186 454 1161 761 1028 2301
Fragments arranged by size:
2301 1161 1028 761 454 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2703 2757 3036 3454 4227 4312 5383 2211
Size: 492 54 279 418 773 85 1071 2994
Fragments arranged by size:
2994 1071 773 492 418 279 85 54
BsmI GAATG_Cn'
Cuts at: 3677 3770 3677
Size: 93 6073
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2194 2211 2703 2757 3036 3143
Size: 399 271 133 17 492 54 279 107
Cuts at: 3143 3454 3485 4227 4312 5383 1391
Size: 311 31 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 311 279 271
133 107 85 54 31 17
BspEI T'CCGG_A
Cuts at: 5829 5829
Size: 6166
BspHI T'CATG_A
Cuts at: 4055 5785 4055
Size: 1730 4436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3426 3521 844
Size: 2582 95 3489
Fragments arranged by size:
3489 2582 95
BsrBI CCG'CTC
Cuts at: 1394 1823 4053 1394
Size: 429 2230 3507
Fragments arranged by size:
3507 2230 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4661 4835 65
Size: 1183 3413 174 1396
Fragments arranged by size:
3413 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 2454 2923 4810 4997 1700
Size: 110 295 349 469 1887 187 2869
Fragments arranged by size:
2869 1887 469 349 295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6166
BssSI C'ACGA_G
Cuts at: 4226 5238 4226
Size: 1012 5154
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2707 2781 794
Size: 1913 74 4179
Fragments arranged by size:
4179 1913 74
BstBI TT'CG_AA
Cuts at: 4970 4970
Size: 6166
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6166
BstXI CCAn_nnnn'nTGG
Cuts at: 2308 2376 2308
Size: 68 6098
BstYI r'GATC_y
Cuts at: 1972 3096 3496 3863 4248 4265 5717 5803 5815
Size: 1124 400 367 385 17 1452 86 12
Cuts at: 5815 6161 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1124 400 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2262 2262
Size: 6166
BtgI C'CryG_G
Cuts at: 513 1065 2039 2061 3174 513
Size: 552 974 22 1113 3505
Fragments arranged by size:
3505 1113 974 552 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5427
Fragments arranged by size:
5427 683 56
BtsI GCAGTG_nn'
Cuts at: 2495 3682 4476 4496 2495
Size: 1187 794 20 4165
Fragments arranged by size:
4165 1187 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6131
DraI TTT'AAA
Cuts at: 3470 3470
Size: 6166
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2756 5173 809
Size: 507 1440 2417 1802
Fragments arranged by size:
2417 1802 1440 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2298 2452
Size: 54 1425 50 275 18 277 191 154
Cuts at: 2452 2949 3084 3162 3171 3435 3613 4502 6131
Size: 497 135 78 9 264 178 889 1629
Cuts at: 6131 8
Size: 43
Fragments arranged by size:
1629 1425 889 497 277 275 264 191
178 154 135 78 54 50 43 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 2452 3613 1537
Size: 275 640 1161 4090
Fragments arranged by size:
4090 1161 640 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2438 2864 4096 1353
Size: 863 222 426 1232 3423
Fragments arranged by size:
3423 1232 863 426 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4753 5127 5273 387
Size: 1064 138 3164 374 146 1280
Fragments arranged by size:
3164 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2270 2398 2642 2701 2705 2717
Size: 393 467 171 128 244 59 4 12
Cuts at: 2717 2834 2914 3026 4229 4826 5613 5881 1239
Size: 117 80 112 1203 597 787 268 1524
Fragments arranged by size:
1524 1203 787 597 467 393 268 244
171 128 117 112 80 59 12 4
EcoO109I rG'GnC_Cy
Cuts at: 2070 2833 3425 2070
Size: 763 592 4811
Fragments arranged by size:
4811 763 592
EcoRI G'AATT_C
Cuts at: 3475 3853 3475
Size: 378 5788
FspI TGC'GCA
Cuts at: 4672 4672
Size: 6166
HaeII r_GCGC'y
Cuts at: 1678 3275 3295 5313 1678
Size: 1597 20 2018 2531
Fragments arranged by size:
2531 2018 1597 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2792 2824 4807
Size: 32 135 32 512 32 676 32 1983
Cuts at: 4807 4839 4881 4913 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1983 676 512 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2476 3510 3756 669
Size: 988 281 538 1034 246 3079
Fragments arranged by size:
3079 1034 988 538 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6166
HpaI GTT'AAC
Cuts at: 3756 3756
Size: 6166
KpnI G_GTAC'C
Cuts at: 2620 3491 2620
Size: 871 5295
MfeI C'AATT_G
Cuts at: 3765 3765
Size: 6166
MluI A'CGCG_T
Cuts at: 6042 6042
Size: 6166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 2873 5280 5464 1305
Size: 1257 311 2407 184 2007
Fragments arranged by size:
2407 2007 1257 311 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2300 3086 10
Size: 54 1425 343 468 786 3090
Fragments arranged by size:
3090 1425 786 468 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2666 2750 2969 3191 4124 4483 4642
Size: 648 1500 84 219 222 933 359 159
Cuts at: 4642 518
Size: 2042
Fragments arranged by size:
2042 1500 933 648 359 222 219 159
84
MspA1I CmG'CkG
Cuts at: 1606 2041 2063 2707 2901 3582 4259 5407 5652
Size: 435 22 644 194 681 677 1148 245
Cuts at: 5652 1606
Size: 2120
Fragments arranged by size:
2120 1148 681 677 644 435 245 194
22
NaeI GCC'GGC
Cuts at: 2107 2456 2925 2107
Size: 349 469 5348
Fragments arranged by size:
5348 469 349
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5614
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6166
NgoMIV G'CCGG_C
Cuts at: 2105 2454 2923 2105
Size: 349 469 5348
Fragments arranged by size:
5348 469 349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6166
NotI GC'GGCC_GC
Cuts at: 3613 3613
Size: 6166
NspI r_CATG'y
Cuts at: 1132 1229 3524 5069 1132
Size: 97 2295 1545 2229
Fragments arranged by size:
2295 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6166
PciI A'CATG_T
Cuts at: 5065 5065
Size: 6166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6166
PfoI T'CCnGG_A
Cuts at: 2445 2682 2445
Size: 237 5929
PmeI GTTT'AAAC
Cuts at: 3470 3470
Size: 6166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 5748 5780 4235
Size: 32 1481 32 4621
Fragments arranged by size:
4621 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6166
PsiI TTA'TAA
Cuts at: 3736 3736
Size: 6166
PspOMI G'GGCC_C
Cuts at: 3139 3139
Size: 6166
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 6134
PstI C_TGCA'G
Cuts at: 830 3518 830
Size: 2688 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6166
PvuII CAG'CTG
Cuts at: 2707 2901 2707
Size: 194 5972
SacII CC_GC'GG
Cuts at: 2042 2064 2042
Size: 22 6144
SalI G'TCGA_C
Cuts at: 1936 3508 1936
Size: 1572 4594
SapI GCTCTTCn'nnn_
Cuts at: 2216 2216
Size: 6166
SbfI CC_TGCA'GG
Cuts at: 3518 3518
Size: 6166
ScaI AGT'ACT
Cuts at: 4414 6126 4414
Size: 1712 4454
SexAI A'CCwGG_T
Cuts at: 3039 3039
Size: 6166
SfcI C'TryA_G
Cuts at: 826 1045 3514 4649 5330 5521 826
Size: 219 2469 1135 681 191 1471
Fragments arranged by size:
2469 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3170 3170
Size: 6166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6166
SmaI CCC'GGG
Cuts at: 1872 3493 1872
Size: 1621 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2798 4270 5171 5433 820
Size: 197 926 855 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 855 262 197
SnaBI TAC'GTA
Cuts at: 493 3957 493
Size: 3464 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6166
SphI G_CATG'C
Cuts at: 1132 3524 1132
Size: 2392 3774
SspI AAT'ATT
Cuts at: 5 52 4090 5
Size: 47 4038 2081
Fragments arranged by size:
4038 2081 47
StuI AGG'CCT
Cuts at: 1517 3149 1517
Size: 1632 4534
StyI C'CwwG_G
Cuts at: 513 1065 3605 513
Size: 552 2540 3074
Fragments arranged by size:
3074 2540 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3065 3411 4193 4210 4363 4548 4993
Size: 1255 1485 346 782 17 153 185 445
Cuts at: 4993 325
Size: 1498
Fragments arranged by size:
1498 1485 1255 782 445 346 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2688 4412 6124
Size: 274 80 33 51 1443 711 1724 1712
Cuts at: 6124 96
Size: 138
Fragments arranged by size:
1724 1712 1443 711 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3320 3352 6126 6158 3320
Size: 32 2774 32 3328
Fragments arranged by size:
3328 2774 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6166
XbaI T'CTAG_A
Cuts at: 3502 3502
Size: 6166
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6166
XmaI C'CCGG_G
Cuts at: 1870 3491 1870
Size: 1621 4545
XmnI GAAnn'nnTTC
Cuts at: 2996 4295 2996
Size: 1299 4867
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3974 276
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspXI RsrII SacI SanDI
SfoI SrfI SwaI XcmI