Restriction map for
pFN21AB9128
(Circular) (Six-base) MAPSORT of: pFN21AB9128.seq Check: 4653 from: 1 to: 7411
pFN21AB9128 7411 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5221 278
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 4754 1937
Size: 1542 1275 4594
Fragments arranged by size:
4594 1542 1275
Acc65I G'GTAC_C
Cuts at: 3193 4732 3193
Size: 1539 5872
AclI AA'CG_TT
Cuts at: 1563 2120 5538 5911 1563
Size: 557 3418 373 3063
Fragments arranged by size:
3418 3063 557 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2412 2604 3962 4427 4623 5474 6858 7126
Size: 780 192 1358 465 196 851 1384 268
Cuts at: 7126 1632
Size: 1917
Fragments arranged by size:
1917 1384 1358 851 780 465 268 196
192
AflII C'TTAA_G
Cuts at: 820 1017 3074 820
Size: 197 2057 5157
Fragments arranged by size:
5157 2057 197
AflIII A'CryG_T
Cuts at: 1203 6310 7287 1203
Size: 5107 977 1327
Fragments arranged by size:
5107 1327 977
AgeI A'CCGG_T
Cuts at: 6242 6242
Size: 7411
AhdI GACnn_n'nnGTC
Cuts at: 6140 6140
Size: 7411
AleI CACnn'nnGTG
Cuts at: 1166 4517 1166
Size: 3351 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3987 1184
Size: 2803 4608
AlwNI CAG_nnn'CTG
Cuts at: 2539 5154 6726 2539
Size: 2615 1572 3224
Fragments arranged by size:
3224 2615 1572
ApaLI G'TGCA_C
Cuts at: 5468 6624 5468
Size: 1156 6255
ApoI r'AATT_y
Cuts at: 1493 2185 2286 2358 2591 2686 2989 3047 3208
Size: 692 101 72 233 95 303 58 161
Cuts at: 3208 3596 4720 4946 5098 7207 1493
Size: 388 1124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 692 388 303 233 226
161 152 101 95 72 58
AseI AT'TA_AT
Cuts at: 160 1032 2454 5965 160
Size: 872 1422 3511 1606
Fragments arranged by size:
3511 1606 1422 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
AvaI C'yCGr_G
Cuts at: 1870 1943 4736 1870
Size: 73 2793 4545
Fragments arranged by size:
4545 2793 73
BamHI G'GATC_C
Cuts at: 4741 5108 4741
Size: 367 7044
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3193 3346 4732 6187 618
Size: 325 217 627 1406 153 1386 1455 1842
Fragments arranged by size:
1842 1455 1406 1386 627 325 217 153
BanII G_rGCy'C
Cuts at: 1391 4730 1391
Size: 3339 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2909 3095 3311 928
Size: 971 1010 186 216 5028
Fragments arranged by size:
5028 1010 971 216 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5602 5636 1903
Size: 34 3665 34 3678
Fragments arranged by size:
3678 3665 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2835 3254 5303 6513 2835
Size: 419 2049 1210 3733
Fragments arranged by size:
3733 2049 1210 419
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7411
BfrBI ATG'CAT
Cuts at: 2869 3964 2869
Size: 1095 6316
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6022 136
Size: 107 122 71 631 605 4350 1525
Fragments arranged by size:
4350 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7406 7406
Size: 7411
BlpI GC'TnA_GC
Cuts at: 4828 4828
Size: 7411
Bme1580I G_kGCm'C
Cuts at: 1790 4053 5472 6628 1790
Size: 2263 1419 1156 2573
Fragments arranged by size:
2573 2263 1419 1156
BmgBI CAC'GTC
Cuts at: 1318 4332 1318
Size: 3014 4397
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2707 4016 5142
Size: 416 53 83 399 24 1290 1309 1126
Cuts at: 5142 6100 442
Size: 958 1753
Fragments arranged by size:
1753 1309 1290 1126 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3370 3630 6071 1239
Size: 2131 260 2441 2579
Fragments arranged by size:
2579 2441 2131 260
Bpu10I CC'TnA_GC
Cuts at: 3069 3069
Size: 7411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2454 2628 3086 3637 5536 6401 6699 2454
Size: 174 458 551 1899 865 298 3166
Fragments arranged by size:
3166 1899 865 551 458 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4675 6074 882
Size: 617 3176 1399 2219
Fragments arranged by size:
3176 2219 1399 617
BsaAI yAC'GTr
Cuts at: 493 1204 3430 5202 493
Size: 711 2226 1772 2702
Fragments arranged by size:
2702 2226 1772 711
BsaBI GATnn'nnATC
Cuts at: 7405 7405
Size: 7411
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5218 5600 6235 275
Size: 53 83 186 936 3685 382 635 1451
Fragments arranged by size:
3685 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5844 6242 6516 6663 7074 5844
Size: 398 274 147 411 6181
Fragments arranged by size:
6181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7186
Fragments arranged by size:
7186 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7411
BseYI C'CCAG_C
Cuts at: 6614 7110 6614
Size: 496 6915
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6838
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4861 5622 6650 1540
Size: 275 186 2860 761 1028 2301
Fragments arranged by size:
2860 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3640 5472 5557 6628 3640
Size: 1832 85 1071 4423
Fragments arranged by size:
4423 1832 1071 85
BsmI GAATG_Cn'
Cuts at: 2056 3761 4922 5015 2056
Size: 1705 1161 93 4452
Fragments arranged by size:
4452 1705 1161 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3640 4053 4730 5472 5557 6628 1391
Size: 399 1850 413 677 742 85 1071 2174
Fragments arranged by size:
2174 1850 1071 742 677 413 399 85
BspEI T'CCGG_A
Cuts at: 7074 7074
Size: 7411
BspHI T'CATG_A
Cuts at: 4677 5300 7030 4677
Size: 623 1730 5058
Fragments arranged by size:
5058 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4618 4766 844
Size: 3774 148 3489
Fragments arranged by size:
3774 3489 148
BsrBI CCG'CTC
Cuts at: 1394 1823 3503 5298 1394
Size: 429 1680 1795 3507
Fragments arranged by size:
3507 1795 1680 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2794 3933 5906 6080 65
Size: 1183 1546 1139 1973 174 1396
Fragments arranged by size:
1973 1546 1396 1183 1139 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6055 6242 1700
Size: 110 4245 187 2869
Fragments arranged by size:
4245 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7411
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7411
BssSI C'ACGA_G
Cuts at: 5471 6483 5471
Size: 1012 6399
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2300 2539 3923 794
Size: 1506 239 1384 4282
Fragments arranged by size:
4282 1506 1384 239
BstBI TT'CG_AA
Cuts at: 3118 6215 3118
Size: 3097 4314
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7411
BstXI CCAn_nnnn'nTGG
Cuts at: 3143 4516 3143
Size: 1373 6038
BstYI r'GATC_y
Cuts at: 1972 3896 4741 5108 5493 5510 6962 7048 7060
Size: 1924 845 367 385 17 1452 86 12
Cuts at: 7060 7406 1972
Size: 346 1977
Fragments arranged by size:
1977 1924 1452 845 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3480 3480
Size: 7411
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5920
Fragments arranged by size:
5920 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6672
Fragments arranged by size:
6672 683 56
BtsI GCAGTG_nn'
Cuts at: 3035 4927 5721 5741 3035
Size: 1892 794 20 4705
Fragments arranged by size:
4705 1892 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7376
DraI TTT'AAA
Cuts at: 3420 4198 4715 3420
Size: 778 517 6116
Fragments arranged by size:
6116 778 517
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2418 6418 809
Size: 507 1102 4000 1802
Fragments arranged by size:
4000 1802 1102 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4858 5747 7376
Size: 54 1425 50 275 18 3028 889 1629
Cuts at: 7376 8
Size: 43
Fragments arranged by size:
3028 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4858 1537
Size: 275 3046 4090
Fragments arranged by size:
4090 3046 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3359 4446 5341 1353
Size: 2006 1087 895 3423
Fragments arranged by size:
3423 2006 1087 895
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5998 6372 6518 387
Size: 1064 138 4409 374 146 1280
Fragments arranged by size:
4409 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2412 2604 3370 3630 3962 4427 4623
Size: 393 780 192 766 260 332 465 196
Cuts at: 4623 5474 6071 6858 7126 1239
Size: 851 597 787 268 1524
Fragments arranged by size:
1524 851 787 780 766 597 465 393
332 268 260 196 192
EcoNI CCTnn'n_nnAGG
Cuts at: 2882 2882
Size: 7411
EcoO109I rG'GnC_Cy
Cuts at: 2856 3659 4671 2856
Size: 803 1012 5596
Fragments arranged by size:
5596 1012 803
EcoRI G'AATT_C
Cuts at: 3596 4720 5098 3596
Size: 1124 378 5909
Fragments arranged by size:
5909 1124 378
FspI TGC'GCA
Cuts at: 3760 5917 3760
Size: 2157 5254
FspAI rTGC'GCAy
Cuts at: 3760 3760
Size: 7411
HaeII r_GCGC'y
Cuts at: 1678 6558 1678
Size: 4880 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2258 2290 2626 2658 2687
Size: 32 135 32 686 32 336 32 29
Cuts at: 2687 2719 3304 3336 3499 3514 3531 3546 6052
Size: 32 585 32 163 15 17 15 2506
Cuts at: 6052 6084 6126 6158 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2506 686 585 336 163 135 42
32 32 32 32 32 32 32 32
29 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4755 5001 669
Size: 988 281 2817 246 3079
Fragments arranged by size:
3079 2817 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2738 3104 748
Size: 1990 366 5055
Fragments arranged by size:
5055 1990 366
HpaI GTT'AAC
Cuts at: 5001 5001
Size: 7411
KpnI G_GTAC'C
Cuts at: 3197 4736 3197
Size: 1539 5872
MfeI C'AATT_G
Cuts at: 4536 5010 4536
Size: 474 6937
MluI A'CGCG_T
Cuts at: 7287 7287
Size: 7411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3238 4187 4204 4671 6525 6709 1305
Size: 1933 949 17 467 1854 184 2007
Fragments arranged by size:
2007 1933 1854 949 467 184 17
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5589
Fragments arranged by size:
5589 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2757 4517 5369 5728 5887 518
Size: 648 1591 1760 852 359 159 2042
Fragments arranged by size:
2042 1760 1591 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2339 4827 5504 6652 6897 1606
Size: 733 2488 677 1148 245 2120
Fragments arranged by size:
2488 2120 1148 733 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5920
Fragments arranged by size:
5920 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7411
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7411
NotI GC'GGCC_GC
Cuts at: 4858 4858
Size: 7411
NsiI A_TGCA'T
Cuts at: 2871 3966 2871
Size: 1095 6316
NspI r_CATG'y
Cuts at: 1132 1229 3173 3282 4769 6314 1132
Size: 97 1944 109 1487 1545 2229
Fragments arranged by size:
2229 1944 1545 1487 109 97
PasI CC'CwG_GG
Cuts at: 1348 2066 1348
Size: 718 6693
PciI A'CATG_T
Cuts at: 6310 6310
Size: 7411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3190 1108
Size: 2082 5329
PfoI T'CCnGG_A
Cuts at: 3669 3768 3669
Size: 99 7312
PmeI GTTT'AAAC
Cuts at: 4715 4715
Size: 7411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3450 3482 5480 5512 6993 7025 3450
Size: 32 1998 32 1481 32 3836
Fragments arranged by size:
3836 1998 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2856 3659 2856
Size: 803 6608
PshAI GACnn'nnGTC
Cuts at: 1613 4687 1613
Size: 3074 4337
PsiI TTA'TAA
Cuts at: 3017 4478 4981 3017
Size: 1461 503 5447
Fragments arranged by size:
5447 1461 503
PstI C_TGCA'G
Cuts at: 830 3042 4594 4763 830
Size: 2212 1552 169 3478
Fragments arranged by size:
3478 2212 1552 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7411
PvuII CAG'CTG
Cuts at: 2339 2339
Size: 7411
SalI G'TCGA_C
Cuts at: 1936 4753 1936
Size: 2817 4594
SbfI CC_TGCA'GG
Cuts at: 4763 4763
Size: 7411
ScaI AGT'ACT
Cuts at: 4483 5659 7371 4483
Size: 1176 1712 4523
Fragments arranged by size:
4523 1712 1176
SfcI C'TryA_G
Cuts at: 826 1045 2178 2637 2879 3038 4590 4759 5894
Size: 219 1133 459 242 159 1552 169 1135
Cuts at: 5894 6575 6766 826
Size: 681 191 1471
Fragments arranged by size:
1552 1471 1135 1133 681 459 242 219
191 169 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7411
SmaI CCC'GGG
Cuts at: 1872 4738 1872
Size: 2866 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2469 2643 3074 3101 3616 5515
Size: 197 926 526 174 431 27 515 1899
Cuts at: 5515 6416 6678 820
Size: 901 262 1553
Fragments arranged by size:
1899 1553 926 901 526 515 431 262
197 174 27
SnaBI TAC'GTA
Cuts at: 493 5202 493
Size: 4709 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7411
SphI G_CATG'C
Cuts at: 1132 4769 1132
Size: 3637 3774
SspI AAT'ATT
Cuts at: 5 52 3060 3707 4354 5335 5
Size: 47 3008 647 647 981 2081
Fragments arranged by size:
3008 2081 981 647 647 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7411
StyI C'CwwG_G
Cuts at: 513 1065 2004 4850 513
Size: 552 939 2846 3074
Fragments arranged by size:
3074 2846 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5438 5455 5608 5793 6238 325
Size: 1255 3858 17 153 185 445 1498
Fragments arranged by size:
3858 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2473 4481 5657
Size: 274 80 33 51 1443 496 2008 1176
Cuts at: 5657 7369 96
Size: 1712 138
Fragments arranged by size:
2008 1712 1443 1176 496 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7371 7403 7371
Size: 32 7379
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7411
XbaI T'CTAG_A
Cuts at: 4747 4747
Size: 7411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4516 4516
Size: 7411
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7411
XmaI C'CCGG_G
Cuts at: 1870 4736 1870
Size: 2866 4545
XmnI GAAnn'nnTTC
Cuts at: 2286 2916 2991 3114 5540 2286
Size: 630 75 123 2426 4157
Fragments arranged by size:
4157 2426 630 123 75
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5219 276
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI