Restriction map for
pFN21AB9123
(Circular) (Six-base) MAPSORT of: pFN21AB9123.seq Check: 8596 from: 1 to: 6220
pFN21AB9123 6220 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3394 3394
Size: 6220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2425 3371 3563 1937
Size: 488 946 192 4594
Fragments arranged by size:
4594 946 488 192
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 3054 3381 3441 4283 5667 5935 1632
Size: 483 939 327 60 842 1384 268 1917
Fragments arranged by size:
1917 1384 939 842 483 327 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 2644 5119 6096 1203
Size: 1441 2475 977 1327
Fragments arranged by size:
2475 1441 1327 977
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 2051 1166
Size: 885 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2166 1184
Size: 982 5238
AlwNI CAG_nnn'CTG
Cuts at: 3963 5535 3963
Size: 1572 4648
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2390 3529 3755 3907 6016 1493
Size: 897 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 897 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3416 4774 160
Size: 872 2384 1358 1606
Fragments arranged by size:
2384 1606 1358 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3545 1870
Size: 73 1602 4545
Fragments arranged by size:
4545 1602 73
BamHI G'GATC_C
Cuts at: 2656 2689 3550 3917 2656
Size: 33 861 367 4959
Fragments arranged by size:
4959 861 367 33
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 3541 4996 618
Size: 325 217 627 1331 423 1455 1842
Fragments arranged by size:
1842 1455 1331 627 423 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2956 3539 1391
Size: 621 944 583 4072
Fragments arranged by size:
4072 944 621 583
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 3522 928
Size: 971 222 1401 3626
Fragments arranged by size:
3626 1401 971 222
BbvCI CC'TCA_GC
Cuts at: 3061 3061
Size: 6220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3263 3297 4411 4445 1903
Size: 34 1326 34 1114 34 3678
Fragments arranged by size:
3678 1326 1114 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4112 5322 3347
Size: 765 1210 4245
Fragments arranged by size:
4245 1210 765
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2225 6215 2225
Size: 3990 2230
BlpI GC'TnA_GC
Cuts at: 3255 3637 3255
Size: 382 5838
Bme1580I G_kGCm'C
Cuts at: 1790 4281 5437 1790
Size: 2491 1156 2573
Fragments arranged by size:
2573 2491 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3951 4909 442
Size: 416 53 83 399 24 2534 958 1753
Fragments arranged by size:
2534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2358 3030 4880 1239
Size: 1119 672 1850 2579
Fragments arranged by size:
2579 1850 1119 672
Bpu10I CC'TnA_GC
Cuts at: 3061 3061
Size: 6220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4345 5210 5508 4345
Size: 865 298 5057
Fragments arranged by size:
5057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4027 4409 5044 275
Size: 53 83 186 936 2494 382 635 1451
Fragments arranged by size:
2494 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2072 2102 2534 2564 1178
Size: 30 165 30 669 30 432 30 4834
Fragments arranged by size:
4834 669 432 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2859 2862 2865 2892 2895 2898 2922 3027
Size: 1673 3 3 27 3 3 24 105
Cuts at: 3027 3049 3138 1186
Size: 22 89 4268
Fragments arranged by size:
4268 1673 105 89 27 24 22 3
3 3 3
BseYI C'CCAG_C
Cuts at: 5423 5919 5423
Size: 496 5724
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2206 2949 3372 1163
Size: 573 426 44 743 423 4011
Fragments arranged by size:
4011 743 573 426 423 44
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2956 3256 4281 4366 5437 2012
Size: 944 300 1025 85 1071 2795
Fragments arranged by size:
2795 1071 1025 944 300 85
BsmI GAATG_Cn'
Cuts at: 3731 3824 3731
Size: 93 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2956 3256 3539 4281 4366 5437
Size: 399 222 944 300 283 742 85 1071
Cuts at: 5437 1391
Size: 2174
Fragments arranged by size:
2174 1071 944 742 399 300 283 222
85
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3394 3575 844
Size: 2550 181 3489
Fragments arranged by size:
3489 2550 181
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4864 5051 1700
Size: 110 3054 187 2869
Fragments arranged by size:
3054 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 2395 5024 2395
Size: 2629 3591
BstEII G'GTnAC_C
Cuts at: 1184 2044 1184
Size: 860 5360
BstXI CCAn_nnnn'nTGG
Cuts at: 2639 2639
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2225 2452 2656 2689 3550 3917 4302 4319
Size: 253 227 204 33 861 367 385 17
Cuts at: 4319 5771 5857 5869 6215 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 861 385 367 346 253 227
204 86 33 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2036 2478 513
Size: 552 971 442 4255
Fragments arranged by size:
4255 971 552 442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 2194 3736 4530 4550 2142
Size: 52 1542 794 20 3812
Fragments arranged by size:
3812 1542 794 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3284 3361 3667
Size: 54 1425 50 275 18 1454 77 306
Cuts at: 3667 4556 6185 8
Size: 889 1629 43
Fragments arranged by size:
1629 1454 1425 889 306 275 77 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2585 2917 4150 1353
Size: 746 486 332 1233 3423
Fragments arranged by size:
3423 1233 746 486 332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4807 5181 5327 387
Size: 1064 138 3218 374 146 1280
Fragments arranged by size:
3218 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2954 2010
Size: 944 5276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2358 3030 3054 3381 3441 4283
Size: 393 483 243 672 24 327 60 842
Cuts at: 4283 4880 5667 5935 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 842 787 672 597 483 393 327
268 243 60 24
EcoO109I rG'GnC_Cy
Cuts at: 2058 2897 3074 2058
Size: 839 177 5204
Fragments arranged by size:
5204 839 177
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2375 3245 2375
Size: 870 5350
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2931 5367 1678
Size: 1253 2436 2531
Fragments arranged by size:
2531 2436 1253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 3019 3051 3321
Size: 32 135 32 500 32 915 32 270
Cuts at: 3321 3353 4861 4893 4935 4967 1373
Size: 32 1508 32 42 32 2626
Fragments arranged by size:
2626 1508 915 500 270 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2426 2542 3564 3810 669
Size: 988 281 488 116 1022 246 3079
Fragments arranged by size:
3079 1022 988 488 281 246 116
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3227 3364 5334 5518 1305
Size: 1922 137 1970 184 2007
Fragments arranged by size:
2007 1970 1922 184 137
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3363 10
Size: 54 1425 343 1531 2867
Fragments arranged by size:
2867 1531 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 4178 4537 4696 518
Size: 648 885 2127 359 159 2042
Fragments arranged by size:
2127 2042 885 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2963 3636 4313 5461 5706 1606
Size: 432 925 673 677 1148 245 2120
Fragments arranged by size:
2120 1148 925 677 673 432 245
NcoI C'CATG_G
Cuts at: 513 1065 2478 513
Size: 552 1413 4255
Fragments arranged by size:
4255 1413 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2648 3578 5123 1132
Size: 97 1419 930 1545 2229
Fragments arranged by size:
2229 1545 1419 930 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 2644 5119 2644
Size: 2475 3745
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6220
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 2897 2058
Size: 839 5381
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PstI C_TGCA'G
Cuts at: 830 3214 3572 830
Size: 2384 358 3478
Fragments arranged by size:
3478 2384 358
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2963 2963
Size: 6220
SacI G_AGCT'C
Cuts at: 2012 2956 2012
Size: 944 5276
SacII CC_GC'GG
Cuts at: 2039 2039
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 2424 3562 1936
Size: 488 1138 4594
Fragments arranged by size:
4594 1138 488
SanDI GG'GwC_CC
Cuts at: 2058 2058
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 2484 3210 3568 4703 5384 5575 826
Size: 219 1439 726 358 1135 681 191 1471
Fragments arranged by size:
1471 1439 1135 726 681 358 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4324 5225 5487 820
Size: 197 926 2381 901 262 1553
Fragments arranged by size:
2381 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 2244 4144 5
Size: 47 2192 1900 2081
Fragments arranged by size:
2192 2081 1900 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2478 2601 3659 513
Size: 552 1413 123 1058 3074
Fragments arranged by size:
3074 1413 1058 552 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3398 3406 4247 4264 4417 4602 5047
Size: 1255 1818 8 841 17 153 185 445
Cuts at: 5047 325
Size: 1498
Fragments arranged by size:
1818 1498 1255 841 445 185 153 17
8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6180 6212 6180
Size: 32 6188
Tth111I GACn'n_nGTC
Cuts at: 1316 2125 1316
Size: 809 5411
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 2708 4349 2708
Size: 1641 4579
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI XcmI