Restriction map for
pFN21AB9104
(Circular) (Six-base) MAPSORT of: pFN21AB9104.seq Check: 478 from: 1 to: 6328
pFN21AB9104 6328 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3204 4138 278
Size: 53 83 186 936 1668 934 2468
Fragments arranged by size:
2468 1668 936 934 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 2432 4455 4828 1563
Size: 869 2023 373 3063
Fragments arranged by size:
3063 2023 869 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3089 4391 5775 6043 1632
Size: 1457 1302 1384 268 1917
Fragments arranged by size:
1917 1457 1384 1302 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6131
AflIII A'CryG_T
Cuts at: 1203 3257 5227 6204 1203
Size: 2054 1970 977 1327
Fragments arranged by size:
2054 1970 1327 977
AgeI A'CCGG_T
Cuts at: 5159 5159
Size: 6328
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 3113 1166
Size: 1947 4381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3131 1184
Size: 1947 4381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2336 2368 2336
Size: 32 6296
AlwNI CAG_nnn'CTG
Cuts at: 2833 4071 5643 2833
Size: 1238 1572 3518
Fragments arranged by size:
3518 1572 1238
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 3637 3863 4015 6124 1493
Size: 2144 226 152 2109 1697
Fragments arranged by size:
2144 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 3653 1870
Size: 73 745 965 4545
Fragments arranged by size:
4545 965 745 73
BamHI G'GATC_C
Cuts at: 2030 3091 3658 4025 2030
Size: 1061 567 367 4333
Fragments arranged by size:
4333 1061 567 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3107 3115 3607 3649 5104
Size: 325 217 627 1320 8 492 42 1455
Cuts at: 5104 618
Size: 1842
Fragments arranged by size:
1842 1455 1320 627 492 325 217 42
8
BanII G_rGCy'C
Cuts at: 1391 2265 2580 2637 2817 3046 3567 3600 3647
Size: 874 315 57 180 229 521 33 47
Cuts at: 3647 1391
Size: 4072
Fragments arranged by size:
4072 874 521 315 229 180 57 47
33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4519 4553 1903
Size: 34 50 34 2498 34 3678
Fragments arranged by size:
3678 2498 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 2243 4939
Size: 107 122 71 631 605 379 192 2696
Cuts at: 4939 136
Size: 1525
Fragments arranged by size:
2696 1525 631 605 379 192 122 107
71
BglII A'GATC_T
Cuts at: 6323 6323
Size: 6328
BlpI GC'TnA_GC
Cuts at: 2217 2493 3745 2217
Size: 276 1252 4800
Fragments arranged by size:
4800 1252 276
Bme1580I G_kGCm'C
Cuts at: 1790 3120 3513 4389 5545 1790
Size: 1330 393 876 1156 2573
Fragments arranged by size:
2573 1330 1156 876 393
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2034 3595 4059
Size: 416 53 83 399 24 617 1561 464
Cuts at: 4059 5017 442
Size: 958 1753
Fragments arranged by size:
1753 1561 958 617 464 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2714
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2171 2195 2240 2303 2732 2855 2891
Size: 917 15 24 45 63 429 123 36
Cuts at: 2891 3242 3443 3551 3566 4988 1239
Size: 351 201 108 15 1422 2579
Fragments arranged by size:
2579 1422 917 429 351 201 123 108
63 45 36 24 15 15
Bpu10I CC'TnA_GC
Cuts at: 3045 3045
Size: 6328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 4453 5318 5616 2594
Size: 1859 865 298 3306
Fragments arranged by size:
3306 1859 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3386 4991 882
Size: 617 1887 1605 2219
Fragments arranged by size:
2219 1887 1605 617
BsaAI yAC'GTr
Cuts at: 493 1204 3260 3364 4119 493
Size: 711 2056 104 755 2702
Fragments arranged by size:
2702 2056 755 711 104
BsaBI GATnn'nnATC
Cuts at: 3096 6322 3096
Size: 3226 3102
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3201 4135 4517 5152
Size: 53 83 186 936 1668 934 382 635
Cuts at: 5152 275
Size: 1451
Fragments arranged by size:
1668 1451 936 934 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4761 5159 5433 5580 5991 4761
Size: 398 274 147 411 5098
Fragments arranged by size:
5098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6103
Fragments arranged by size:
6103 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2852 3005 3494 1186
Size: 967 699 153 489 4020
Fragments arranged by size:
4020 967 699 489 153
BseYI C'CCAG_C
Cuts at: 2330 2621 2810 2822 3209 3290 3618 5531 6027
Size: 291 189 12 387 81 328 1913 496
Cuts at: 6027 2330
Size: 2631
Fragments arranged by size:
2631 1913 496 387 328 291 189 81
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 1163
Size: 573 468 5287
Fragments arranged by size:
5287 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3137 3348 3778 4539 5567 1540
Size: 275 186 1136 211 430 761 1028 2301
Fragments arranged by size:
2301 1136 1028 761 430 275 211 186
BsiHKAI G_wGCw'C
Cuts at: 2580 3567 4389 4474 5545 2580
Size: 987 822 85 1071 3363
Fragments arranged by size:
3363 1071 987 822 85
BsmI GAATG_Cn'
Cuts at: 2469 2959 3839 3932 2469
Size: 490 880 93 4865
Fragments arranged by size:
4865 880 490 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2135 2279 3460 2135
Size: 144 1181 5003
Fragments arranged by size:
5003 1181 144
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2580 2637 2817 3046 3120 3513
Size: 399 475 315 57 180 229 74 393
Cuts at: 3513 3567 3600 3647 4389 4474 5545 1391
Size: 54 33 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 399 393 315 229
180 85 74 57 54 47 33
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 2971 4217 5947 2971
Size: 1246 1730 3352
Fragments arranged by size:
3352 1730 1246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3683 844
Size: 2839 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3151 4215 1394
Size: 429 1328 1064 3507
Fragments arranged by size:
3507 1328 1064 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2593 4823 4997 65
Size: 1183 1345 2230 174 1396
Fragments arranged by size:
2230 1396 1345 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4972 5159 1700
Size: 110 3162 187 2869
Fragments arranged by size:
3162 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6328
BssHII G'CGCG_C
Cuts at: 1925 2465 1925
Size: 540 5788
BssSI C'ACGA_G
Cuts at: 4388 5400 4388
Size: 1012 5316
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2503 794
Size: 1709 4619
BstBI TT'CG_AA
Cuts at: 5132 5132
Size: 6328
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstYI r'GATC_y
Cuts at: 1972 2030 2108 2190 2613 3091 3658 4025 4410
Size: 58 78 82 423 478 567 367 385
Cuts at: 4410 4427 5879 5965 5977 6323 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 567 478 423 385 367 346
86 82 78 58 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2046 3111 3181 3576 513
Size: 552 981 1065 70 395 3265
Fragments arranged by size:
3265 1065 981 552 395 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 2560 2567 2605 2812 3844 4638 4658 2560
Size: 7 38 207 1032 794 20 4230
Fragments arranged by size:
4230 1032 794 207 38 20 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2575 2610 529
Size: 35 2011 35 4247
Fragments arranged by size:
4247 2011 35 35
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5335 809
Size: 507 4019 1802
Fragments arranged by size:
4019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 3134 3345
Size: 54 1425 50 275 18 285 1019 211
Cuts at: 3345 3775 4664 6293 8
Size: 430 889 1629 43
Fragments arranged by size:
1629 1425 1019 889 430 285 275 211
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3134 3345 3775 1537
Size: 275 1322 211 430 4090
Fragments arranged by size:
4090 1322 430 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 4258 1353
Size: 2905 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3005 3473 4915 5289 5435 387
Size: 1064 138 1416 468 1442 374 146 1280
Fragments arranged by size:
1442 1416 1280 1064 468 374 146 138
EcoICRI GAG'CTC
Cuts at: 2578 3565 2578
Size: 987 5341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2171 2195 2240 2303 2732 2855
Size: 393 524 15 24 45 63 429 123
Cuts at: 2855 2891 3089 3242 3443 3551 3566 4391 4988
Size: 36 198 153 201 108 15 825 597
Cuts at: 4988 5775 6043 1239
Size: 787 268 1524
Fragments arranged by size:
1524 825 787 597 524 429 393 268
201 198 153 123 108 63 45 36
24 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2117 2117
Size: 6328
EcoO109I rG'GnC_Cy
Cuts at: 2200 2866 2892 3379 3552 2200
Size: 666 26 487 173 4976
Fragments arranged by size:
4976 666 487 173 26
EcoRI G'AATT_C
Cuts at: 3637 4015 3637
Size: 378 5950
FspI TGC'GCA
Cuts at: 2387 2585 4834 2387
Size: 198 2249 3881
Fragments arranged by size:
3881 2249 198
FspAI rTGC'GCAy
Cuts at: 2387 2387
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 3217 5475 1678
Size: 1539 2258 2531
Fragments arranged by size:
2531 2258 1539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3028 3060 4969 5001 5043
Size: 32 135 32 1456 32 1909 32 42
Cuts at: 5043 5075 1373
Size: 32 2626
Fragments arranged by size:
2626 1909 1456 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3316 3672 3918 669
Size: 988 281 1378 356 246 3079
Fragments arranged by size:
3079 1378 988 356 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6328
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5442 5626 1305
Size: 4137 184 2007
Fragments arranged by size:
4137 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 10
Size: 54 1425 343 285 4221
Fragments arranged by size:
4221 1425 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2424 2514 3113 4286 4645 4804 518
Size: 648 1258 90 599 1173 359 159 2042
Fragments arranged by size:
2042 1258 1173 648 599 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2212 2830 3499 3618 3744 4421 5569 5814
Size: 606 618 669 119 126 677 1148 245
Cuts at: 5814 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 669 618 606 245 126
119
NcoI C'CATG_G
Cuts at: 513 1065 3576 513
Size: 552 2511 3265
Fragments arranged by size:
3265 2511 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 2513 2790 3007 3686 5231 1132
Size: 97 1284 277 217 679 1545 2229
Fragments arranged by size:
2229 1545 1284 679 277 217 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6328
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2778 2833 1108
Size: 1670 55 4603
Fragments arranged by size:
4603 1670 55
PfoI T'CCnGG_A
Cuts at: 2016 2256 2280 2016
Size: 240 24 6064
Fragments arranged by size:
6064 240 24
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 5910 5942 4397
Size: 32 1481 32 4783
Fragments arranged by size:
4783 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2866 2892 3552 2200
Size: 666 26 660 4976
Fragments arranged by size:
4976 666 660 26
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PstI C_TGCA'G
Cuts at: 830 2059 2574 3680 830
Size: 1229 515 1106 3478
Fragments arranged by size:
3478 1229 1106 515
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
PvuII CAG'CTG
Cuts at: 2212 2830 3618 2212
Size: 618 788 4922
Fragments arranged by size:
4922 788 618
SacI G_AGCT'C
Cuts at: 2580 3567 2580
Size: 987 5341
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SanDI GG'GwC_CC
Cuts at: 2892 2892
Size: 6328
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SfcI C'TryA_G
Cuts at: 826 1045 2055 2570 3676 4811 5492 5683 826
Size: 219 1010 515 1106 1135 681 191 1471
Fragments arranged by size:
1471 1135 1106 1010 681 515 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 3655 1872
Size: 1783 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2609 4432 5333 5595 820
Size: 197 926 666 1823 901 262 1553
Fragments arranged by size:
1823 1553 926 901 666 262 197
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 2790 3007 3686 1132
Size: 1658 217 679 3774
Fragments arranged by size:
3774 1658 679 217
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 2502 2721 1517
Size: 985 219 5124
Fragments arranged by size:
5124 985 219
StyI C'CwwG_G
Cuts at: 513 1065 2118 3576 3767 513
Size: 552 1053 1458 191 3074
Fragments arranged by size:
3074 1458 1053 552 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2414 2504 3595 3610 4355 4372 4525
Size: 1255 834 90 1091 15 745 17 153
Cuts at: 4525 4710 5155 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1091 834 745 445 185 153
90 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4574 6286 96
Size: 274 80 33 51 1443 2597 1712 138
Fragments arranged by size:
2597 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 6288 6320 2258
Size: 32 3998 32 2266
Fragments arranged by size:
3998 2266 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2830 3277 2338
Size: 492 447 5389
Fragments arranged by size:
5389 492 447
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6328
XmaI C'CCGG_G
Cuts at: 1870 3653 1870
Size: 1783 4545
XmnI GAAnn'nnTTC
Cuts at: 4457 4457
Size: 6328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3202 4136 276
Size: 53 83 186 936 1668 934 2468
Fragments arranged by size:
2468 1668 936 934 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI