Restriction map for
pFN21AB9098
(Circular) (Six-base) MAPSORT of: pFN21AB9098.seq Check: 5328 from: 1 to: 8476
pFN21AB9098 8476 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2448 2448
Size: 8476
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2862 6286 278
Size: 53 83 186 936 1326 3424 2468
Fragments arranged by size:
3424 2468 1326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5819 1937
Size: 3882 4594
Acc65I G'GTAC_C
Cuts at: 5797 5797
Size: 8476
AclI AA'CG_TT
Cuts at: 1563 6603 6976 1563
Size: 5040 373 3063
Fragments arranged by size:
5040 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2284 2546 2945 3104 3856 4315 4419 4560
Size: 652 262 399 159 752 459 104 141
Cuts at: 4560 5055 5223 6539 7923 8191 1632
Size: 495 168 1316 1384 268 1917
Fragments arranged by size:
1917 1384 1316 752 652 495 459 399
268 262 168 159 141 104
AfeI AGC'GCT
Cuts at: 4440 4440
Size: 8476
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8279
AflIII A'CryG_T
Cuts at: 1203 7375 8352 1203
Size: 6172 977 1327
Fragments arranged by size:
6172 1327 977
AgeI A'CCGG_T
Cuts at: 7307 7307
Size: 8476
AhdI GACnn_n'nnGTC
Cuts at: 4623 7205 4623
Size: 2582 5894
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3917 1184
Size: 2733 5743
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 8444
AlwNI CAG_nnn'CTG
Cuts at: 2152 2779 3043 6219 7791 2152
Size: 627 264 3176 1572 2837
Fragments arranged by size:
3176 2837 1572 627 264
ApaLI G'TGCA_C
Cuts at: 6533 7689 6533
Size: 1156 7320
ApoI r'AATT_y
Cuts at: 1493 3799 4570 5785 6011 6163 8272 1493
Size: 2306 771 1215 226 152 2109 1697
Fragments arranged by size:
2306 2109 1697 1215 771 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2420 7030 160
Size: 872 1388 4610 1606
Fragments arranged by size:
4610 1606 1388 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8476
AvaI C'yCGr_G
Cuts at: 1870 1943 2176 2191 5801 1870
Size: 73 233 15 3610 4545
Fragments arranged by size:
4545 3610 233 73 15
BamHI G'GATC_C
Cuts at: 2016 3617 5806 6173 2016
Size: 1601 2189 367 4319
Fragments arranged by size:
4319 2189 1601 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3400 3435 4703 5797 7252
Size: 325 217 627 1613 35 1268 1094 1455
Cuts at: 7252 618
Size: 1842
Fragments arranged by size:
1842 1613 1455 1268 1094 627 325 217
35
BanII G_rGCy'C
Cuts at: 1391 2183 3189 3777 4092 4457 4881 4932 5055
Size: 792 1006 588 315 365 424 51 123
Cuts at: 5055 5795 1391
Size: 740 4072
Fragments arranged by size:
4072 1006 792 740 588 424 365 315
123 51
BbeI G_GCGC'C
Cuts at: 3439 3439
Size: 8476
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2471 2607 2678 3997 4434 5107 5589
Size: 971 572 136 71 1319 437 673 482
Cuts at: 5589 5736 928
Size: 147 3668
Fragments arranged by size:
3668 1319 971 673 572 482 437 147
136 71
BbvCI CC'TCA_GC
Cuts at: 2330 2757 4280 2330
Size: 427 1523 6526
Fragments arranged by size:
6526 1523 427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3316 3350 4876 4910 6667
Size: 34 50 34 1295 34 1526 34 1757
Cuts at: 6667 6701 1903
Size: 34 3678
Fragments arranged by size:
3678 1757 1526 1295 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6368 7578 6368
Size: 1210 7266
BclI T'GATC_A
Cuts at: 1548 3330 3825 4489 1548
Size: 1782 495 664 5535
Fragments arranged by size:
5535 1782 664 495
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2194 4658 7087
Size: 107 122 71 631 605 522 2464 2429
Cuts at: 7087 136
Size: 1525
Fragments arranged by size:
2464 2429 1525 631 605 522 122 107
71
BglII A'GATC_T
Cuts at: 2907 8471 2907
Size: 5564 2912
BlpI GC'TnA_GC
Cuts at: 4442 5049 5893 4442
Size: 607 844 7025
Fragments arranged by size:
7025 844 607
Bme1580I G_kGCm'C
Cuts at: 1790 3405 4706 6537 7693 1790
Size: 1615 1301 1831 1156 2573
Fragments arranged by size:
2573 1831 1615 1301 1156
BmgBI CAC'GTC
Cuts at: 1318 2038 5397 1318
Size: 720 3359 4397
Fragments arranged by size:
4397 3359 720
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4846 6207 7165
Size: 416 53 83 399 24 3429 1361 958
Cuts at: 7165 442
Size: 1753
Fragments arranged by size:
3429 1753 1361 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8476
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 5371 2191
Size: 3180 5296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2291 2771 2923 3146 3272 3543 3956
Size: 989 63 480 152 223 126 271 413
Cuts at: 3956 4106 5033 5096 5366 7136 1239
Size: 150 927 63 270 1770 2579
Fragments arranged by size:
2579 1770 989 927 480 413 271 270
223 152 150 126 63 63
Bpu10I CC'TnA_GC
Cuts at: 2090 2330 2757 3962 4280 4738 2090
Size: 240 427 1205 318 458 5828
Fragments arranged by size:
5828 1205 458 427 318 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2534 6601 7466 7764 2534
Size: 4067 865 298 3246
Fragments arranged by size:
4067 3246 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3122 3450 4859 7139 882
Size: 617 1623 328 1409 2280 2219
Fragments arranged by size:
2280 2219 1623 1409 617 328
BsaAI yAC'GTr
Cuts at: 493 1204 2770 6267 493
Size: 711 1566 3497 2702
Fragments arranged by size:
3497 2702 1566 711
BsaBI GATnn'nnATC
Cuts at: 2513 4488 8470 2513
Size: 1975 3982 2519
Fragments arranged by size:
3982 2519 1975
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2859 3436 3837 5567
Size: 53 83 186 936 1326 577 401 1730
Cuts at: 5567 6283 6665 7300 275
Size: 716 382 635 1451
Fragments arranged by size:
1730 1451 1326 936 716 635 577 401
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2694 3303 4725 6909 7307 7581 7728 8139 2694
Size: 609 1422 2184 398 274 147 411 3031
Fragments arranged by size:
3031 2184 1422 609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3245 3275 4079 4109 4522
Size: 30 165 30 1842 30 804 30 413
Cuts at: 4522 4552 5751 5781 1178
Size: 30 1199 30 3873
Fragments arranged by size:
3873 1842 1199 804 413 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3128 3269 3599 3617 4159 4490 5030 5150
Size: 1942 141 330 18 542 331 540 120
Cuts at: 5150 5573 1186
Size: 423 4089
Fragments arranged by size:
4089 1942 542 540 423 331 330 141
120 18
BseYI C'CCAG_C
Cuts at: 7679 8175 7679
Size: 496 7980
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2651 2765 2792 3556 3974 4767
Size: 573 684 231 114 27 764 418 793
Cuts at: 4767 1163
Size: 4872
Fragments arranged by size:
4872 793 764 684 573 418 231 114
27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2227 4662 5926 6687 7715 1540
Size: 275 186 226 2435 1264 761 1028 2301
Fragments arranged by size:
2435 2301 1264 1028 761 275 226 186
BsiHKAI G_wGCw'C
Cuts at: 2183 3189 3213 4092 4457 4830 4881 4932 5676
Size: 1006 24 879 365 373 51 51 744
Cuts at: 5676 6537 6622 7693 2183
Size: 861 85 1071 2966
Fragments arranged by size:
2966 1071 1006 879 861 744 373 365
85 51 51 24
BsmI GAATG_Cn'
Cuts at: 5987 6080 5987
Size: 93 8383
BsmBI CGTCTCn'nnnn_
Cuts at: 3284 3284
Size: 8476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 3189 3213 3405 3777 4092 4457
Size: 399 393 1006 24 192 372 315 365
Cuts at: 4457 4706 4830 4881 4932 5055 5676 5795 6537
Size: 249 124 51 51 123 621 119 742
Cuts at: 6537 6622 7693 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1006 742 621 399 393 372
365 315 249 192 124 123 119 85
51 51 24
BspEI T'CCGG_A
Cuts at: 3303 8139 3303
Size: 4836 3640
BspHI T'CATG_A
Cuts at: 3205 3777 4614 5193 6365 8095 3205
Size: 572 837 579 1172 1730 3586
Fragments arranged by size:
3586 1730 1172 837 579 572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2211 2442 2448 3960 5831 844
Size: 1316 51 231 6 1512 1871 3489
Fragments arranged by size:
3489 1871 1512 1316 231 51 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2521 3928 4945 6363 1394
Size: 429 698 1407 1017 1418 3507
Fragments arranged by size:
3507 1418 1407 1017 698 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2373 3775 5260 6971 7145 65
Size: 1183 1125 1402 1485 1711 174 1396
Fragments arranged by size:
1711 1485 1402 1396 1183 1125 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2245 2658 4215 7120 7307 1700
Size: 110 435 413 1557 2905 187 2869
Fragments arranged by size:
2905 2869 1557 435 413 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8476
BssHII G'CGCG_C
Cuts at: 1925 2060 2229 4150 1925
Size: 135 169 1921 6251
Fragments arranged by size:
6251 1921 169 135
BssSI C'ACGA_G
Cuts at: 6536 7548 6536
Size: 1012 7464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2152 2375 2443 2779 794
Size: 1358 223 68 336 6491
Fragments arranged by size:
6491 1358 336 223 68
BstBI TT'CG_AA
Cuts at: 7280 7280
Size: 8476
BstEII G'GTnAC_C
Cuts at: 1184 2697 1184
Size: 1513 6963
BstXI CCAn_nnnn'nTGG
Cuts at: 4084 4772 4084
Size: 688 7788
BstYI r'GATC_y
Cuts at: 1972 2016 2907 3039 3156 3348 3617 3906 5806
Size: 44 891 132 117 192 269 289 1900
Cuts at: 5806 6173 6558 6575 8027 8113 8125 8471 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1900 1452 891 385 367 346 289
269 192 132 117 86 44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2469 513
Size: 552 1404 6520
Fragments arranged by size:
6520 1404 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7737
Fragments arranged by size:
7737 683 56
BtsI GCAGTG_nn'
Cuts at: 3049 4654 5377 5992 6786 6806 3049
Size: 1605 723 615 794 20 4719
Fragments arranged by size:
4719 1605 794 723 615 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2111 2146 529
Size: 35 1547 35 6859
Fragments arranged by size:
6859 1547 35 35
DraI TTT'AAA
Cuts at: 3649 5780 3649
Size: 2131 6345
DraIII CAC_nnn'GTG
Cuts at: 4840 5513 4840
Size: 673 7803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5591 7483 809
Size: 507 4275 1892 1802
Fragments arranged by size:
4275 1892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2256 2472
Size: 54 1425 50 275 18 202 224 216
Cuts at: 2472 2820 3315 3622 4105 4272 4317 4659 5298
Size: 348 495 307 483 167 45 342 639
Cuts at: 5298 5923 6812 8441 8
Size: 625 889 1629 43
Fragments arranged by size:
1629 1425 889 639 625 495 483 348
342 307 275 224 216 202 167 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4659 5923 1537
Size: 275 2847 1264 4090
Fragments arranged by size:
4090 2847 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 6406 1353
Size: 809 4244 3423
Fragments arranged by size:
4244 3423 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2677 4234 4820 7063 7437 7583
Size: 1064 138 1088 1557 586 2243 374 146
Cuts at: 7583 387
Size: 1280
Fragments arranged by size:
2243 1557 1280 1088 1064 586 374 146
138
EcoICRI GAG'CTC
Cuts at: 2181 3187 4090 4455 4879 4930 2181
Size: 1006 903 365 424 51 5727
Fragments arranged by size:
5727 1006 903 424 365 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2284 2291 2546 2771 2923 2945
Size: 393 596 56 7 255 225 152 22
Cuts at: 2945 3104 3146 3272 3543 3856 3956 4106 4315
Size: 159 42 126 271 313 100 150 209
Cuts at: 4315 4419 4560 5033 5055 5096 5223 5366 6539
Size: 104 141 473 22 41 127 143 1173
Cuts at: 6539 7136 7923 8191 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1173 787 597 596 473 393 313
271 268 255 225 209 159 152 150
143 141 127 126 104 100 56 42
41 22 22 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2207 2549 3395 4937 2207
Size: 342 846 1542 5746
Fragments arranged by size:
5746 1542 846 342
EcoO109I rG'GnC_Cy
Cuts at: 2394 4261 4987 5181 2394
Size: 1867 726 194 5689
Fragments arranged by size:
5689 1867 726 194
EcoRI G'AATT_C
Cuts at: 5785 6163 5785
Size: 378 8098
EcoRV GAT'ATC
Cuts at: 5619 5619
Size: 8476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3553 5202 3553
Size: 1649 6827
FspI TGC'GCA
Cuts at: 2720 6982 2720
Size: 4262 4214
HaeII r_GCGC'y
Cuts at: 1678 2252 3439 4442 5283 7623 1678
Size: 574 1187 1003 841 2340 2531
Fragments arranged by size:
2531 2340 1187 1003 841 574
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2525 2557 3245
Size: 32 135 32 593 32 328 32 688
Cuts at: 3245 3277 4609 4641 7117 7149 7191 7223 1373
Size: 32 1332 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 1332 688 593 328 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2794 3862 5596 5820 6066 669
Size: 988 281 856 1068 1734 224 246 3079
Fragments arranged by size:
3079 1734 1068 988 856 281 246 224
HindIII A'AGCT_T
Cuts at: 748 3530 5424 5447 748
Size: 2782 1894 23 3777
Fragments arranged by size:
3777 2782 1894 23
HpaI GTT'AAC
Cuts at: 6066 6066
Size: 8476
KasI G'GCGC_C
Cuts at: 3435 3435
Size: 8476
KpnI G_GTAC'C
Cuts at: 5801 5801
Size: 8476
MfeI C'AATT_G
Cuts at: 6075 6075
Size: 8476
MluI A'CGCG_T
Cuts at: 8352 8352
Size: 8476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3858 4565 7590 7774 1305
Size: 2553 707 3025 184 2007
Fragments arranged by size:
3025 2553 2007 707 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2034 2474 3317 4107 10
Size: 54 1425 343 202 440 843 790 4379
Fragments arranged by size:
4379 1425 843 790 440 343 202 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3398 3695 4307 5027 5214 6434 6793
Size: 648 2232 297 612 720 187 1220 359
Cuts at: 6793 6952 518
Size: 159 2042
Fragments arranged by size:
2232 2042 1220 720 648 612 359 297
187 159
MspA1I CmG'CkG
Cuts at: 1606 2269 2779 2812 2943 4284 5092 5319 5892
Size: 663 510 33 131 1341 808 227 573
Cuts at: 5892 6569 7717 7962 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1341 1148 808 677 663 573 510
245 227 131 33
NaeI GCC'GGC
Cuts at: 2247 2660 4217 2247
Size: 413 1557 6506
Fragments arranged by size:
6506 1557 413
NarI GG'CG_CC
Cuts at: 3436 3436
Size: 8476
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7924
NdeI CA'TA_TG
Cuts at: 387 5312 387
Size: 4925 3551
NgoMIV G'CCGG_C
Cuts at: 2245 2658 4215 2245
Size: 413 1557 6506
Fragments arranged by size:
6506 1557 413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8476
NotI GC'GGCC_GC
Cuts at: 5923 5923
Size: 8476
NspI r_CATG'y
Cuts at: 1132 1229 2384 2719 2749 4312 5834 7379 1132
Size: 97 1155 335 30 1563 1522 1545 2229
Fragments arranged by size:
2229 1563 1545 1522 1155 335 97 30
PasI CC'CwG_GG
Cuts at: 1348 2047 1348
Size: 699 7777
PciI A'CATG_T
Cuts at: 7375 7375
Size: 8476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3751 1108
Size: 2643 5833
PfoI T'CCnGG_A
Cuts at: 2682 3354 4026 4902 2682
Size: 672 672 876 6256
Fragments arranged by size:
6256 876 672 672
PmeI GTTT'AAAC
Cuts at: 5780 5780
Size: 8476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3370 3402 5751 5783 6545 6577 8058 8090 3370
Size: 32 2349 32 762 32 1481 32 3756
Fragments arranged by size:
3756 2349 1481 762 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2394 4987 5181 2394
Size: 2593 194 5689
Fragments arranged by size:
5689 2593 194
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8476
PsiI TTA'TAA
Cuts at: 6046 6046
Size: 8476
PspXI vC'TCGA_Gb
Cuts at: 2176 2176
Size: 8476
PstI C_TGCA'G
Cuts at: 830 2148 2448 3039 3906 4957 5828 830
Size: 1318 300 591 867 1051 871 3478
Fragments arranged by size:
3478 1318 1051 871 867 591 300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8476
PvuII CAG'CTG
Cuts at: 2779 2943 4284 5092 2779
Size: 164 1341 808 6163
Fragments arranged by size:
6163 1341 808 164
SacI G_AGCT'C
Cuts at: 2183 3189 4092 4457 4881 4932 2183
Size: 1006 903 365 424 51 5727
Fragments arranged by size:
5727 1006 903 424 365 51
SalI G'TCGA_C
Cuts at: 1936 5818 1936
Size: 3882 4594
SapI GCTCTTCn'nnn_
Cuts at: 2162 2162
Size: 8476
SbfI CC_TGCA'GG
Cuts at: 5828 5828
Size: 8476
ScaI AGT'ACT
Cuts at: 6724 8436 6724
Size: 1712 6764
SexAI A'CCwGG_T
Cuts at: 2730 2730
Size: 8476
SfcI C'TryA_G
Cuts at: 826 1045 2144 2389 2444 3035 3902 4953 5824
Size: 219 1099 245 55 591 867 1051 871
Cuts at: 5824 6959 7640 7831 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1099 1051 871 867 681 591
245 219 191 55
SfoI GGC'GCC
Cuts at: 3437 3437
Size: 8476
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8476
SgrAI Cr'CCGG_yG
Cuts at: 1700 4215 1700
Size: 2515 5961
SmaI CCC'GGG
Cuts at: 1872 5803 1872
Size: 3931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2176 2549 6580 7481 7743 820
Size: 197 926 233 373 4031 901 262 1553
Fragments arranged by size:
4031 1553 926 901 373 262 233 197
SnaBI TAC'GTA
Cuts at: 493 6267 493
Size: 5774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8476
SphI G_CATG'C
Cuts at: 1132 2719 2749 5834 1132
Size: 1587 30 3085 3774
Fragments arranged by size:
3774 3085 1587 30
SspI AAT'ATT
Cuts at: 5 52 5696 6400 5
Size: 47 5644 704 2081
Fragments arranged by size:
5644 2081 704 47
StuI AGG'CCT
Cuts at: 1517 2922 1517
Size: 1405 7071
StyI C'CwwG_G
Cuts at: 513 1065 2259 2322 2917 3804 5915 513
Size: 552 1194 63 595 887 2111 3074
Fragments arranged by size:
3074 2111 1194 887 595 552 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2353 2591 4564 4828 5284 6503 6520
Size: 1255 773 238 1973 264 456 1219 17
Cuts at: 6520 6673 6858 7303 325
Size: 153 185 445 1498
Fragments arranged by size:
1973 1498 1255 1219 773 456 445 264
238 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3027 4311 6722
Size: 274 80 33 51 1443 1050 1284 2411
Cuts at: 6722 8434 96
Size: 1712 138
Fragments arranged by size:
2411 1712 1443 1284 1050 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 4826 4858 5499 5531 8436 8468 2027
Size: 32 2767 32 641 32 2905 32 2035
Fragments arranged by size:
2905 2767 2035 641 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2858 4201 1316
Size: 1542 1343 5591
Fragments arranged by size:
5591 1542 1343
XbaI T'CTAG_A
Cuts at: 5812 5812
Size: 8476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2956 3247 3436 4018 4024 4505 2266
Size: 690 291 189 582 6 481 6237
Fragments arranged by size:
6237 690 582 481 291 189 6
XhoI C'TCGA_G
Cuts at: 1943 2176 1943
Size: 233 8243
XmaI C'CCGG_G
Cuts at: 1870 5801 1870
Size: 3931 4545
XmnI GAAnn'nnTTC
Cuts at: 6605 6605
Size: 8476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2860 6284 276
Size: 53 83 186 936 1326 3424 2468
Fragments arranged by size:
3424 2468 1326 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI
RsrII SacII SanDI SfiI SrfI SwaI