Restriction map for
pFN21AB9075
(Circular) (Six-base) MAPSORT of: pFN21AB9075.seq Check: 8835 from: 1 to: 8719
pFN21AB9075 8719 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3019 3019
Size: 8719
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6529 278
Size: 53 83 186 936 4993 2468
Fragments arranged by size:
4993 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2401 3927 6062 1937
Size: 464 1526 2135 4594
Fragments arranged by size:
4594 2135 1526 464
Acc65I G'GTAC_C
Cuts at: 6040 6040
Size: 8719
AclI AA'CG_TT
Cuts at: 1563 6846 7219 1563
Size: 5283 373 3063
Fragments arranged by size:
5283 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 3213 5627 5789 6782 8166 8434 1632
Size: 840 741 2414 162 993 1384 268 1917
Fragments arranged by size:
2414 1917 1384 993 840 741 268 162
AfeI AGC'GCT
Cuts at: 5774 5774
Size: 8719
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8522
AflIII A'CryG_T
Cuts at: 1203 5247 7618 8595 1203
Size: 4044 2371 977 1327
Fragments arranged by size:
4044 2371 1327 977
AgeI A'CCGG_T
Cuts at: 7550 7550
Size: 8719
AhdI GACnn_n'nnGTC
Cuts at: 5251 7448 5251
Size: 2197 6522
AleI CACnn'nnGTG
Cuts at: 1166 2978 3665 4301 4564 1166
Size: 1812 687 636 263 5321
Fragments arranged by size:
5321 1812 687 636 263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8719
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4512 4544 4512
Size: 32 8687
AlwNI CAG_nnn'CTG
Cuts at: 2016 2149 2369 5880 6462 8034 2016
Size: 133 220 3511 582 1572 2701
Fragments arranged by size:
3511 2701 1572 582 220 133
ApaLI G'TGCA_C
Cuts at: 6776 7932 6776
Size: 1156 7563
ApoI r'AATT_y
Cuts at: 1493 3588 6028 6254 6406 8515 1493
Size: 2095 2440 226 152 2109 1697
Fragments arranged by size:
2440 2109 2095 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7273 160
Size: 872 6241 1606
Fragments arranged by size:
6241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8719
AvaI C'yCGr_G
Cuts at: 1870 1943 4067 4473 5136 6044 1870
Size: 73 2124 406 663 908 4545
Fragments arranged by size:
4545 2124 908 663 406 73
BamHI G'GATC_C
Cuts at: 6049 6416 6049
Size: 367 8352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3408 3899 6040 7495
Size: 325 217 627 330 1291 491 2141 1455
Cuts at: 7495 618
Size: 1842
Fragments arranged by size:
2141 1842 1455 1291 627 491 330 325
217
BanII G_rGCy'C
Cuts at: 1391 2466 4220 4910 4983 5368 6038 1391
Size: 1075 1754 690 73 385 670 4072
Fragments arranged by size:
4072 1754 1075 690 670 385 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4353 5037 928
Size: 971 2454 684 4610
Fragments arranged by size:
4610 2454 971 684
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3075 3109 4883 4917 6910 6944 1903
Size: 34 1138 34 1774 34 1993 34 3678
Fragments arranged by size:
3678 1993 1774 1138 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 6611 7821 3389
Size: 3222 1210 4287
Fragments arranged by size:
4287 3222 1210
BclI T'GATC_A
Cuts at: 1548 4009 4281 1548
Size: 2461 272 5986
Fragments arranged by size:
5986 2461 272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7330 136
Size: 107 122 71 631 605 5658 1525
Fragments arranged by size:
5658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4996 8714 4996
Size: 3718 5001
BlpI GC'TnA_GC
Cuts at: 2048 2225 3224 5940 6136 2048
Size: 177 999 2716 196 4631
Fragments arranged by size:
4631 2716 999 196 177
Bme1580I G_kGCm'C
Cuts at: 1790 5901 6780 7936 1790
Size: 4111 879 1156 2573
Fragments arranged by size:
4111 2573 1156 879
BmgBI CAC'GTC
Cuts at: 1318 5248 5902 1318
Size: 3930 654 4135
Fragments arranged by size:
4135 3930 654
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 2714 3350
Size: 416 53 83 399 24 609 688 636
Cuts at: 3350 4499 5868 6450 7408 442
Size: 1149 1369 582 958 1753
Fragments arranged by size:
1753 1369 1149 958 688 636 609 582
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3564 1056
Size: 2508 6211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3537 3537
Size: 8719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2821 3932 4295 7379 1239
Size: 1582 1111 363 3084 2579
Fragments arranged by size:
3084 2579 1582 1111 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2280 4751 5795 5842 6844 7709 8007 2280
Size: 2471 1044 47 1002 865 298 2992
Fragments arranged by size:
2992 2471 1044 1002 865 298 47
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5301 5415 7382 882
Size: 617 3802 114 1967 2219
Fragments arranged by size:
3802 2219 1967 617 114
BsaAI yAC'GTr
Cuts at: 493 1204 4566 6510 493
Size: 711 3362 1944 2702
Fragments arranged by size:
3362 2702 1944 711
BsaBI GATnn'nnATC
Cuts at: 8713 8713
Size: 8719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6526 6908 7543 275
Size: 53 83 186 936 4993 382 635 1451
Fragments arranged by size:
4993 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7152 7550 7824 7971 8382 7152
Size: 398 274 147 411 7489
Fragments arranged by size:
7489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2139 2169 3975 4005 5027
Size: 30 165 30 736 30 1806 30 1022
Cuts at: 5027 5057 1178
Size: 30 4840
Fragments arranged by size:
4840 1806 1022 736 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4967 1186
Size: 3781 4938
BseYI C'CCAG_C
Cuts at: 2121 4687 7922 8418 2121
Size: 2566 3235 496 2422
Fragments arranged by size:
3235 2566 2422 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4681 1163
Size: 573 2945 5201
Fragments arranged by size:
5201 2945 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6169 6930 7958 1540
Size: 275 186 4168 761 1028 2301
Fragments arranged by size:
4168 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2466 3849 4910 5368 6780 6865 7936 2466
Size: 1383 1061 458 1412 85 1071 3249
Fragments arranged by size:
3249 1412 1383 1071 1061 458 85
BsmI GAATG_Cn'
Cuts at: 2999 5058 5710 6230 6323 2999
Size: 2059 652 520 93 5395
Fragments arranged by size:
5395 2059 652 520 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 8719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2466 3849 4220 4910 4983 5368 5901
Size: 399 676 1383 371 690 73 385 533
Cuts at: 5901 6038 6780 6865 7936 1391
Size: 137 742 85 1071 2174
Fragments arranged by size:
2174 1383 1071 742 690 676 533 399
385 371 137 85 73
BspEI T'CCGG_A
Cuts at: 8382 8382
Size: 8719
BspHI T'CATG_A
Cuts at: 3873 5661 6608 8338 3873
Size: 1788 947 1730 4254
Fragments arranged by size:
4254 1788 1730 947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3019 3136 4692 5910 6074 844
Size: 2175 117 1556 1218 164 3489
Fragments arranged by size:
3489 2175 1556 1218 164 117
BsrBI CCG'CTC
Cuts at: 1394 1823 4438 6606 1394
Size: 429 2615 2168 3507
Fragments arranged by size:
3507 2615 2168 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7214 7388 65
Size: 1183 5966 174 1396
Fragments arranged by size:
5966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3063 7363 7550 1700
Size: 110 1253 4300 187 2869
Fragments arranged by size:
4300 2869 1253 187 110
BsrGI T'GTAC_A
Cuts at: 96 2418 5488 96
Size: 2322 3070 3327
Fragments arranged by size:
3327 3070 2322
BssHII G'CGCG_C
Cuts at: 1925 2040 1925
Size: 115 8604
BssSI C'ACGA_G
Cuts at: 2782 3159 6779 7791 2782
Size: 377 3620 1012 3710
Fragments arranged by size:
3710 3620 1012 377
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2131 794
Size: 1337 7382
BstBI TT'CG_AA
Cuts at: 7523 7523
Size: 8719
BstEII G'GTnAC_C
Cuts at: 1184 3123 1184
Size: 1939 6780
BstXI CCAn_nnnn'nTGG
Cuts at: 4990 5032 4990
Size: 42 8677
BstYI r'GATC_y
Cuts at: 1972 2069 2095 4996 6049 6416 6801 6818 8270
Size: 97 26 2901 1053 367 385 17 1452
Cuts at: 8270 8356 8368 8714 1972
Size: 86 12 346 1977
Fragments arranged by size:
2901 1977 1452 1053 385 367 346 97
86 26 17 12
BstZ17I GTA'TAC
Cuts at: 3928 3928
Size: 8719
Bsu36I CC'TnA_GG
Cuts at: 5287 5342 5287
Size: 55 8664
BtgI C'CryG_G
Cuts at: 513 1065 2004 4605 513
Size: 552 939 2601 4627
Fragments arranged by size:
4627 2601 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7980
Fragments arranged by size:
7980 683 56
BtsI GCAGTG_nn'
Cuts at: 2147 3258 4192 4436 6235 7029 7049 2147
Size: 1111 934 244 1799 794 20 3817
Fragments arranged by size:
3817 1799 1111 934 794 244 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3253 3288 529
Size: 35 2689 35 5960
Fragments arranged by size:
5960 2689 35 35
DraI TTT'AAA
Cuts at: 2112 2511 3250 4090 6023 2112
Size: 399 739 840 1933 4808
Fragments arranged by size:
4808 1933 840 739 399
DraIII CAC_nnn'GTG
Cuts at: 4018 4018
Size: 8719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2027 7726 809
Size: 507 711 5699 1802
Fragments arranged by size:
5699 1802 711 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3831 4020 4608
Size: 54 1425 50 275 18 2001 189 588
Cuts at: 4608 4660 5022 6166 7055 8684 8
Size: 52 362 1144 889 1629 43
Fragments arranged by size:
2001 1629 1425 1144 889 588 362 275
189 54 52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6166 1537
Size: 275 4354 4090
Fragments arranged by size:
4354 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3189 3459 4167 4322 4501 4944 6649 1353
Size: 1836 270 708 155 179 443 1705 3423
Fragments arranged by size:
3423 1836 1705 708 443 270 179 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7306 7680 7826 387
Size: 1064 138 5717 374 146 1280
Fragments arranged by size:
5717 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2464 4908 5366 2464
Size: 2444 458 5817
Fragments arranged by size:
5817 2444 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2472 2821 3213 3932 4295 5627 5789
Size: 393 840 349 392 719 363 1332 162
Cuts at: 5789 6782 7379 8166 8434 1239
Size: 993 597 787 268 1524
Fragments arranged by size:
1524 1332 993 840 787 719 597 393
392 363 349 268 162
EcoO109I rG'GnC_Cy
Cuts at: 2029 3718 4712 5068 2029
Size: 1689 994 356 5680
Fragments arranged by size:
5680 1689 994 356
EcoRI G'AATT_C
Cuts at: 6028 6406 6028
Size: 378 8341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4380 4530 5836 4380
Size: 150 1306 7263
Fragments arranged by size:
7263 1306 150
FspI TGC'GCA
Cuts at: 7225 7225
Size: 8719
HaeII r_GCGC'y
Cuts at: 1678 5776 7866 1678
Size: 4098 2090 2531
Fragments arranged by size:
4098 2531 2090
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2137 2169 2647 2679 4373
Size: 32 135 32 565 32 478 32 1694
Cuts at: 4373 4405 5027 5059 5237 5269 5350 5382 5972
Size: 32 622 32 178 32 81 32 590
Cuts at: 5972 6004 7360 7392 7434 7466 1373
Size: 32 1356 32 42 32 2626
Fragments arranged by size:
2626 1694 1356 622 590 565 478 178
135 81 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2971 3688 3742 4681 6063 6309
Size: 988 281 1033 717 54 939 1382 246
Cuts at: 6309 669
Size: 3079
Fragments arranged by size:
3079 1382 1033 988 939 717 281 246
54
HindIII A'AGCT_T
Cuts at: 748 3649 4897 748
Size: 2901 1248 4570
Fragments arranged by size:
4570 2901 1248
HpaI GTT'AAC
Cuts at: 2971 3688 3742 4681 6309 2971
Size: 717 54 939 1628 5381
Fragments arranged by size:
5381 1628 939 717 54
KpnI G_GTAC'C
Cuts at: 6044 6044
Size: 8719
MfeI C'AATT_G
Cuts at: 2755 6318 2755
Size: 3563 5156
MluI A'CGCG_T
Cuts at: 8595 8595
Size: 8719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 7833 8017 1305
Size: 3477 3051 184 2007
Fragments arranged by size:
3477 3051 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3833 4022 4662 5024 10
Size: 54 1425 343 2001 189 640 362 3705
Fragments arranged by size:
3705 2001 1425 640 362 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2978 2988 3008 3665 4056 4301 4564
Size: 648 1812 10 20 657 391 245 263
Cuts at: 4564 4988 5357 5714 6677 7036 7195 518
Size: 424 369 357 963 359 159 2042
Fragments arranged by size:
2042 1812 963 657 648 424 391 369
359 357 263 245 159 20 10
MspA1I CmG'CkG
Cuts at: 1606 2052 2322 5892 6135 6812 7960 8205 1606
Size: 446 270 3570 243 677 1148 245 2120
Fragments arranged by size:
3570 2120 1148 677 446 270 245 243
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7228
Fragments arranged by size:
7228 939 552
NdeI CA'TA_TG
Cuts at: 387 3373 387
Size: 2986 5733
NheI G'CTAG_C
Cuts at: 1052 3560 1052
Size: 2508 6211
NotI GC'GGCC_GC
Cuts at: 6166 6166
Size: 8719
NspI r_CATG'y
Cuts at: 1132 1229 4296 6077 7622 1132
Size: 97 3067 1781 1545 2229
Fragments arranged by size:
3067 2229 1781 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8719
PciI A'CATG_T
Cuts at: 7618 7618
Size: 8719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4461 1108
Size: 3353 5366
PfoI T'CCnGG_A
Cuts at: 3477 3477
Size: 8719
PmeI GTTT'AAAC
Cuts at: 6023 6023
Size: 8719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6788 6820 8301 8333 6788
Size: 32 1481 32 7174
Fragments arranged by size:
7174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2029 3718 5068 2029
Size: 1689 1350 5680
Fragments arranged by size:
5680 1689 1350
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8719
PsiI TTA'TAA
Cuts at: 4397 5148 6289 4397
Size: 751 1141 6827
Fragments arranged by size:
6827 1141 751
PstI C_TGCA'G
Cuts at: 830 2273 3914 6071 830
Size: 1443 1641 2157 3478
Fragments arranged by size:
3478 2157 1641 1443
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8719
PvuII CAG'CTG
Cuts at: 2052 2322 5892 2052
Size: 270 3570 4879
Fragments arranged by size:
4879 3570 270
SacI G_AGCT'C
Cuts at: 2466 4910 5368 2466
Size: 2444 458 5817
Fragments arranged by size:
5817 2444 458
SalI G'TCGA_C
Cuts at: 1936 6061 1936
Size: 4125 4594
SbfI CC_TGCA'GG
Cuts at: 6071 6071
Size: 8719
ScaI AGT'ACT
Cuts at: 2275 5375 6967 8679 2275
Size: 3100 1592 1712 2315
Fragments arranged by size:
3100 2315 1712 1592
SfcI C'TryA_G
Cuts at: 826 1045 2269 2317 2881 3808 3910 5230 6067
Size: 219 1224 48 564 927 102 1320 837
Cuts at: 6067 7202 7883 8074 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1320 1224 1135 927 837 681 564
219 191 102 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8719
SmaI CCC'GGG
Cuts at: 1872 4475 5138 6046 1872
Size: 2603 663 908 4545
Fragments arranged by size:
4545 2603 908 663
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2295 4766 5810 5821 6823 7724
Size: 197 926 352 2471 1044 11 1002 901
Cuts at: 7724 7986 820
Size: 262 1553
Fragments arranged by size:
2471 1553 1044 1002 926 901 352 262
197 11
SnaBI TAC'GTA
Cuts at: 493 6510 493
Size: 6017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8719
SphI G_CATG'C
Cuts at: 1132 6077 1132
Size: 4945 3774
SspI AAT'ATT
Cuts at: 5 52 6643 5
Size: 47 6591 2081
Fragments arranged by size:
6591 2081 47
StuI AGG'CCT
Cuts at: 1517 4848 5804 1517
Size: 3331 956 4432
Fragments arranged by size:
4432 3331 956
StyI C'CwwG_G
Cuts at: 513 1065 2004 3567 5799 6158 513
Size: 552 939 1563 2232 359 3074
Fragments arranged by size:
3074 2232 1563 939 552 359
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 6028 6746 6763 6916 7101 7546
Size: 1255 555 3893 718 17 153 185 445
Cuts at: 7546 325
Size: 1498
Fragments arranged by size:
3893 1498 1255 718 555 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2273 2418 2759
Size: 274 80 33 51 1443 296 145 341
Cuts at: 2759 4617 5311 5373 5488 6000 6965 8677 96
Size: 1858 694 62 115 512 965 1712 138
Fragments arranged by size:
1858 1712 1443 965 694 512 341 296
274 145 138 115 80 62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2139 2171 3975 4007 4415 4447 8679 8711 2139
Size: 32 1804 32 408 32 4232 32 2147
Fragments arranged by size:
4232 2147 1804 408 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8719
XbaI T'CTAG_A
Cuts at: 3584 6055 3584
Size: 2471 6248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5891 5891
Size: 8719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8719
XmaI C'CCGG_G
Cuts at: 1870 4473 5136 6044 1870
Size: 2603 663 908 4545
Fragments arranged by size:
4545 2603 908 663
XmnI GAAnn'nnTTC
Cuts at: 5774 6848 5774
Size: 1074 7645
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6527 276
Size: 53 83 186 936 4993 2468
Fragments arranged by size:
4993 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI