Restriction map for
pFN21AB9023
(Circular) (Six-base) MAPSORT of: pFN21AB9023.seq Check: 2719 from: 1 to: 8830
pFN21AB9023 8830 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3818 4555 3818
Size: 737 8093
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6640 278
Size: 53 83 186 936 5104 2468
Fragments arranged by size:
5104 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3201 5257 6102 6173 1937
Size: 1264 2056 845 71 4594
Fragments arranged by size:
4594 2056 1264 845 71
Acc65I G'GTAC_C
Cuts at: 4377 4982 6151 4377
Size: 605 1169 7056
Fragments arranged by size:
7056 1169 605
AclI AA'CG_TT
Cuts at: 1563 6957 7330 1563
Size: 5394 373 3063
Fragments arranged by size:
5394 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 2729 2887 3342 3345 3600 3802 5372
Size: 1013 84 158 455 3 255 202 1570
Cuts at: 5372 5589 5944 6893 8277 8545 1632
Size: 217 355 949 1384 268 1917
Fragments arranged by size:
1917 1570 1384 1013 949 455 355 268
255 217 202 158 84 3
AfeI AGC'GCT
Cuts at: 5066 5066
Size: 8830
AflII C'TTAA_G
Cuts at: 820 1017 3376 820
Size: 197 2359 6274
Fragments arranged by size:
6274 2359 197
AflIII A'CryG_T
Cuts at: 1203 4308 7729 8706 1203
Size: 3105 3421 977 1327
Fragments arranged by size:
3421 3105 1327 977
AgeI A'CCGG_T
Cuts at: 7661 7661
Size: 8830
AhdI GACnn_n'nnGTC
Cuts at: 5930 7559 5930
Size: 1629 7201
AleI CACnn'nnGTG
Cuts at: 1166 3656 4280 4311 5094 1166
Size: 2490 624 31 783 4902
Fragments arranged by size:
4902 2490 783 624 31
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4738 4857 4928 5231 5348 1184
Size: 3554 119 71 303 117 4666
Fragments arranged by size:
4666 3554 303 119 117 71
AlwNI CAG_nnn'CTG
Cuts at: 2146 3833 4174 5534 6573 8145 2146
Size: 1687 341 1360 1039 1572 2831
Fragments arranged by size:
2831 1687 1572 1360 1039 341
ApaI G_GGCC'C
Cuts at: 3134 4873 3134
Size: 1739 7091
ApaLI G'TGCA_C
Cuts at: 3644 4672 6887 8043 3644
Size: 1028 2215 1156 4431
Fragments arranged by size:
4431 2215 1156 1028
ApoI r'AATT_y
Cuts at: 1493 3976 5548 5802 6139 6365 6517 8626 1493
Size: 2483 1572 254 337 226 152 2109 1697
Fragments arranged by size:
2483 2109 1697 1572 337 254 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7384 160
Size: 872 6352 1606
Fragments arranged by size:
6352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8830
AvaI C'yCGr_G
Cuts at: 1870 1943 2530 2673 3868 5106 6155 1870
Size: 73 587 143 1195 1238 1049 4545
Fragments arranged by size:
4545 1238 1195 1049 587 143 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3227 3260 3227
Size: 33 8797
BamHI G'GATC_C
Cuts at: 2787 3088 5713 6160 6527 2787
Size: 301 2625 447 367 5090
Fragments arranged by size:
5090 2625 447 367 301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2237 3958 4329 4377 4665
Size: 325 217 627 450 1721 371 48 288
Cuts at: 4665 4982 5099 5222 5240 5324 5378 6151 7606
Size: 317 117 123 18 84 54 773 1455
Cuts at: 7606 618
Size: 1842
Fragments arranged by size:
1842 1721 1455 773 627 450 371 325
317 288 217 123 117 84 54 48
18
BanII G_rGCy'C
Cuts at: 1391 2112 3134 3159 4873 5037 6149 1391
Size: 721 1022 25 1714 164 1112 4072
Fragments arranged by size:
4072 1714 1112 1022 721 164 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3414 3427 928
Size: 971 1515 13 6331
Fragments arranged by size:
6331 1515 971 13
BbvCI CC'TCA_GC
Cuts at: 3903 3903
Size: 8830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2179 2213 7021 7055 1903
Size: 34 242 34 4808 34 3678
Fragments arranged by size:
4808 3678 242 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3049 6722 7932 3049
Size: 3673 1210 3947
Fragments arranged by size:
3947 3673 1210
BclI T'GATC_A
Cuts at: 1548 4536 5808 1548
Size: 2988 1272 4570
Fragments arranged by size:
4570 2988 1272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2474 3704 3774
Size: 107 122 71 631 605 802 1230 70
Cuts at: 3774 7441 136
Size: 3667 1525
Fragments arranged by size:
3667 1525 1230 802 631 605 122 107
71 70
BglII A'GATC_T
Cuts at: 5683 8825 5683
Size: 3142 5688
BlpI GC'TnA_GC
Cuts at: 2170 6247 2170
Size: 4077 4753
Bme1580I G_kGCm'C
Cuts at: 1790 2240 3134 3648 4668 4676 4873 5127 5225
Size: 450 894 514 1020 8 197 254 98
Cuts at: 5225 5327 6891 8047 1790
Size: 102 1564 1156 2573
Fragments arranged by size:
2573 1564 1156 1020 894 514 450 254
197 102 98 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4846 5122 5531
Size: 416 53 83 399 24 3429 276 409
Cuts at: 5531 6561 7519 442
Size: 1030 958 1753
Fragments arranged by size:
3429 1753 1030 958 416 409 399 276
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 8830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2814 5089 5105 5918 6022 7490 1239
Size: 1575 2275 16 813 104 1468 2579
Fragments arranged by size:
2579 2275 1575 1468 813 104 16
Bpu10I CC'TnA_GC
Cuts at: 3903 3929 3903
Size: 26 8804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4792 5933 6955 7820 8118 4792
Size: 1141 1022 865 298 5504
Fragments arranged by size:
5504 1141 1022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2853 3167 7493 882
Size: 617 1354 314 4326 2219
Fragments arranged by size:
4326 2219 1354 617 314
BsaAI yAC'GTr
Cuts at: 493 1204 2233 3441 3628 5122 6621 493
Size: 711 1029 1208 187 1494 1499 2702
Fragments arranged by size:
2702 1499 1494 1208 1029 711 187
BsaBI GATnn'nnATC
Cuts at: 6057 8824 6057
Size: 2767 6063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6637 7019 7654 275
Size: 53 83 186 936 5104 382 635 1451
Fragments arranged by size:
5104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4279 7263 7661 7935 8082 8493 4279
Size: 2984 398 274 147 411 4616
Fragments arranged by size:
4616 2984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3273 3303 4004 4010 4034
Size: 30 165 30 1870 30 701 6 24
Cuts at: 4034 4040 4567 4597 4975 5005 5254 5284 5998
Size: 6 527 30 378 30 249 30 714
Cuts at: 5998 6028 1178
Size: 30 3980
Fragments arranged by size:
3980 1870 714 701 527 378 249 165
30 30 30 30 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 2984 3017 3734 3890 6019 1186
Size: 1333 465 33 717 156 2129 3997
Fragments arranged by size:
3997 2129 1333 717 465 156 33
BseYI C'CCAG_C
Cuts at: 2401 3184 3313 3970 4167 4589 5232 5871 8033
Size: 783 129 657 197 422 643 639 2162
Cuts at: 8033 8529 2401
Size: 496 2702
Fragments arranged by size:
2702 2162 783 657 643 639 496 422
197 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2183 3490 4150 5237 1163
Size: 573 345 102 1307 660 1087 4756
Fragments arranged by size:
4756 1307 1087 660 573 345 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2033 5544 6280 7041 8069 1540
Size: 275 186 32 3511 736 761 1028 2301
Fragments arranged by size:
3511 2301 1028 761 736 275 186 32
BsiHKAI G_wGCw'C
Cuts at: 2112 3648 4585 4676 5091 6891 6976 8047 2112
Size: 1536 937 91 415 1800 85 1071 2895
Fragments arranged by size:
2895 1800 1536 1071 937 415 91 85
BsmI GAATG_Cn'
Cuts at: 4694 6341 6434 4694
Size: 1647 93 7090
Fragments arranged by size:
7090 1647 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3872 5531 3872
Size: 1659 7171
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2240 3134 3159 3648 4585 4668
Size: 399 322 128 894 25 489 937 83
Cuts at: 4668 4676 4873 5037 5091 5127 5225 5327 6149
Size: 8 197 164 54 36 98 102 822
Cuts at: 6149 6891 6976 8047 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 937 894 822 742 489 399
322 197 164 128 102 98 85 83
54 36 25 8
BspEI T'CCGG_A
Cuts at: 8493 8493
Size: 8830
BspHI T'CATG_A
Cuts at: 4969 6719 8449 4969
Size: 1750 1730 5350
Fragments arranged by size:
5350 1750 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2479 3672 3818 4555 4730 5088 6185 844
Size: 1635 1193 146 737 175 358 1097 3489
Fragments arranged by size:
3489 1635 1193 1097 737 358 175 146
BsrBI CCG'CTC
Cuts at: 1394 1823 5229 6717 1394
Size: 429 3406 1488 3507
Fragments arranged by size:
3507 3406 1488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3691 7325 7499 65
Size: 1183 2443 3634 174 1396
Fragments arranged by size:
3634 2443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 4518 4655 7474 7661 1700
Size: 110 222 2486 137 2819 187 2869
Fragments arranged by size:
2869 2819 2486 222 187 137 110
BsrGI T'GTAC_A
Cuts at: 96 5903 96
Size: 5807 3023
BssHII G'CGCG_C
Cuts at: 1925 2425 2447 1925
Size: 500 22 8308
Fragments arranged by size:
8308 500 22
BssSI C'ACGA_G
Cuts at: 2347 6890 7902 2347
Size: 4543 1012 3275
Fragments arranged by size:
4543 3275 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3574 3833 794
Size: 2780 259 5791
Fragments arranged by size:
5791 2780 259
BstBI TT'CG_AA
Cuts at: 3343 7634 3343
Size: 4291 4539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8830
BstXI CCAn_nnnn'nTGG
Cuts at: 4388 5357 5437 5710 5896 4388
Size: 969 80 273 186 7322
Fragments arranged by size:
7322 969 273 186 80
BstYI r'GATC_y
Cuts at: 1972 2787 3088 5683 5713 6160 6527 6912 6929
Size: 815 301 2595 30 447 367 385 17
Cuts at: 6929 8381 8467 8479 8825 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2595 1977 1452 815 447 385 367 346
301 86 30 17 12
BstZ17I GTA'TAC
Cuts at: 5258 5258
Size: 8830
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 2175 2353 4612 5167 5277
Size: 552 939 84 87 178 2259 555 110
Cuts at: 5277 513
Size: 4066
Fragments arranged by size:
4066 2259 939 555 552 178 110 87
84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2305 4418 4674 5148 5689 487
Size: 683 56 1079 2113 256 474 541 3628
Fragments arranged by size:
3628 2113 1079 683 541 474 256 56
BtsI GCAGTG_nn'
Cuts at: 2403 3535 5168 6346 7140 7160 2403
Size: 1132 1633 1178 794 20 4073
Fragments arranged by size:
4073 1633 1178 1132 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8795
DraI TTT'AAA
Cuts at: 3141 6134 3141
Size: 2993 5837
DraIII CAC_nnn'GTG
Cuts at: 3598 4823 3598
Size: 1225 7605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 7837 809
Size: 507 4097 2424 1802
Fragments arranged by size:
4097 2424 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2030 2316
Size: 54 1425 50 275 18 192 8 286
Cuts at: 2316 2409 2475 3016 3616 3705 5297 6040 6277
Size: 93 66 541 600 89 1592 743 237
Cuts at: 6277 7166 8795 8
Size: 889 1629 43
Fragments arranged by size:
1629 1592 1425 889 743 600 541 286
275 237 192 93 89 66 54 50
43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2030 6277 1537
Size: 275 218 4247 4090
Fragments arranged by size:
4247 4090 275 218
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2884 5678 6760 1353
Size: 764 767 2794 1082 3423
Fragments arranged by size:
3423 2794 1082 767 764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2351 7417 7791 7937 387
Size: 1064 138 762 5066 374 146 1280
Fragments arranged by size:
5066 1280 1064 762 374 146 138
EcoICRI GAG'CTC
Cuts at: 2110 2110
Size: 8830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2645 2729 2814 2887 3342 3345 3600
Size: 393 1013 84 85 73 455 3 255
Cuts at: 3600 3802 5089 5105 5372 5589 5918 5944 6022
Size: 202 1287 16 267 217 329 26 78
Cuts at: 6022 6893 7490 8277 8545 1239
Size: 871 597 787 268 1524
Fragments arranged by size:
1524 1287 1013 871 787 597 455 393
329 268 267 255 217 202 85 84
78 73 26 16 3
EcoO109I rG'GnC_Cy
Cuts at: 3130 3739 4869 5046 5668 3130
Size: 609 1130 177 622 6292
Fragments arranged by size:
6292 1130 622 609 177
EcoRI G'AATT_C
Cuts at: 5548 5802 6139 6517 5548
Size: 254 337 378 7861
Fragments arranged by size:
7861 378 337 254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5197 5689 5974 5197
Size: 492 285 8053
Fragments arranged by size:
8053 492 285
FspI TGC'GCA
Cuts at: 4369 7336 4369
Size: 2967 5863
FspAI rTGC'GCAy
Cuts at: 4369 4369
Size: 8830
HaeII r_GCGC'y
Cuts at: 1678 2096 2499 2525 4840 5068 5643 7977 1678
Size: 418 403 26 2315 228 575 2334 2531
Fragments arranged by size:
2531 2334 2315 575 418 403 228 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2352 2384 2696
Size: 32 135 32 581 32 167 32 312
Cuts at: 2696 2728 4355 4387 4676 4708 4790 4822 5484
Size: 32 1627 32 289 32 82 32 662
Cuts at: 5484 5516 5840 5872 5916 5948 6066 6098 7471
Size: 32 324 32 44 32 118 32 1373
Cuts at: 7471 7503 7545 7577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1627 1373 662 581 324 312 289
167 135 118 82 44 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4760 6174 6420 669
Size: 988 281 2822 1414 246 3079
Fragments arranged by size:
3079 2822 1414 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8830
HpaI GTT'AAC
Cuts at: 6420 6420
Size: 8830
KpnI G_GTAC'C
Cuts at: 4381 4986 6155 4381
Size: 605 1169 7056
Fragments arranged by size:
7056 1169 605
MfeI C'AATT_G
Cuts at: 3759 6429 3759
Size: 2670 6160
MluI A'CGCG_T
Cuts at: 8706 8706
Size: 8830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2811 2836 3447 4047 7944 8128 1305
Size: 1506 25 611 600 3897 184 2007
Fragments arranged by size:
3897 2007 1506 611 600 184 25
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 3018 3618 6042 10
Size: 54 1425 343 645 541 600 2424 2798
Fragments arranged by size:
2798 2424 1425 645 600 541 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4280 4311 4437 4578 5094 5435
Size: 648 2490 624 31 126 141 516 341
Cuts at: 5435 5708 6788 7147 7306 518
Size: 273 1080 359 159 2042
Fragments arranged by size:
2490 2042 1080 648 624 516 359 341
273 159 141 126 31
MspA1I CmG'CkG
Cuts at: 1606 2090 2849 2870 4174 4632 6246 6923 8071
Size: 484 759 21 1304 458 1614 677 1148
Cuts at: 8071 8316 1606
Size: 245 2120
Fragments arranged by size:
2120 1614 1304 1148 759 677 484 458
245 21
NaeI GCC'GGC
Cuts at: 4657 4657
Size: 8830
NcoI C'CATG_G
Cuts at: 513 1065 2004 4612 5277 513
Size: 552 939 2608 665 4066
Fragments arranged by size:
4066 2608 939 665 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8830
NgoMIV G'CCGG_C
Cuts at: 4655 4655
Size: 8830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8830
NotI GC'GGCC_GC
Cuts at: 6277 6277
Size: 8830
NspI r_CATG'y
Cuts at: 1132 1229 3500 3671 4312 6188 7733 1132
Size: 97 2271 171 641 1876 1545 2229
Fragments arranged by size:
2271 2229 1876 1545 641 171 97
PasI CC'CwG_GG
Cuts at: 1348 2271 2890 3159 4865 1348
Size: 923 619 269 1706 5313
Fragments arranged by size:
5313 1706 923 619 269
PciI A'CATG_T
Cuts at: 4308 7729 4308
Size: 3421 5409
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3850 1108
Size: 2742 6088
PfoI T'CCnGG_A
Cuts at: 5622 5622
Size: 8830
PmeI GTTT'AAAC
Cuts at: 6134 6134
Size: 8830
PmlI CAC'GTG
Cuts at: 3628 3628
Size: 8830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6899 6931 8412 8444 6899
Size: 32 1481 32 7285
Fragments arranged by size:
7285 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5668 5668
Size: 8830
PshAI GACnn'nnGTC
Cuts at: 1613 4150 1613
Size: 2537 6293
PsiI TTA'TAA
Cuts at: 6400 6400
Size: 8830
PspOMI G'GGCC_C
Cuts at: 3130 4869 3130
Size: 1739 7091
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4103 4135 4103
Size: 32 8798
PstI C_TGCA'G
Cuts at: 830 2070 2151 2619 2854 4717 5163 6100 6182
Size: 1240 81 468 235 1863 446 937 82
Cuts at: 6182 830
Size: 3478
Fragments arranged by size:
3478 1863 1240 937 468 446 235 82
81
PvuI CG_AT'CG
Cuts at: 2001 5544 2001
Size: 3543 5287
PvuII CAG'CTG
Cuts at: 2849 4174 4632 2849
Size: 1325 458 7047
Fragments arranged by size:
7047 1325 458
SacI G_AGCT'C
Cuts at: 2112 2112
Size: 8830
SacII CC_GC'GG
Cuts at: 2091 2091
Size: 8830
SalI G'TCGA_C
Cuts at: 1936 6172 1936
Size: 4236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2117 2117
Size: 8830
SbfI CC_TGCA'GG
Cuts at: 2151 6182 2151
Size: 4031 4799
ScaI AGT'ACT
Cuts at: 7078 8790 7078
Size: 1712 7118
SexAI A'CCwGG_T
Cuts at: 4122 4122
Size: 8830
SfcI C'TryA_G
Cuts at: 826 1045 2066 2147 2615 2850 2913 4477 4713
Size: 219 1021 81 468 235 63 1564 236
Cuts at: 4713 4919 5159 6096 6178 7313 7994 8185 826
Size: 206 240 937 82 1135 681 191 1471
Fragments arranged by size:
1564 1471 1135 1021 937 681 468 240
236 235 219 206 191 82 81 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8830
SmaI CCC'GGG
Cuts at: 1872 3870 5108 6157 1872
Size: 1998 1238 1049 4545
Fragments arranged by size:
4545 1998 1238 1049
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3376 4807 5948 6934 7835 8097
Size: 197 926 1433 1431 1141 986 901 262
Cuts at: 8097 820
Size: 1553
Fragments arranged by size:
1553 1433 1431 1141 986 926 901 262
197
SnaBI TAC'GTA
Cuts at: 493 6621 493
Size: 6128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8830
SphI G_CATG'C
Cuts at: 1132 3500 3671 6188 1132
Size: 2368 171 2517 3774
Fragments arranged by size:
3774 2517 2368 171
SspI AAT'ATT
Cuts at: 5 52 6754 5
Size: 47 6702 2081
Fragments arranged by size:
6702 2081 47
StuI AGG'CCT
Cuts at: 1517 3768 1517
Size: 2251 6579
StyI C'CwwG_G
Cuts at: 513 1065 2004 2623 3084 4612 5277 6269 513
Size: 552 939 619 461 1528 665 992 3074
Fragments arranged by size:
3074 1528 992 939 665 619 552 461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 2477 4181 5054 5290 6857 6874
Size: 1255 535 362 1704 873 236 1567 17
Cuts at: 6874 7027 7212 7657 325
Size: 153 185 445 1498
Fragments arranged by size:
1704 1567 1498 1255 873 535 445 362
236 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5472 5604 5733
Size: 274 80 33 51 1443 3495 132 129
Cuts at: 5733 5903 7076 8788 96
Size: 170 1173 1712 138
Fragments arranged by size:
3495 1712 1443 1173 274 170 138 132
129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3073 3105 3273 3305 3527 3559 3598 3630 5254
Size: 32 168 32 222 32 39 32 1624
Cuts at: 5254 5265 5286 5297 5602 5634 5998 6030 8790
Size: 11 21 11 305 32 364 32 2760
Cuts at: 8790 8822 3073
Size: 32 3081
Fragments arranged by size:
3081 2760 1624 364 305 222 168 39
32 32 32 32 32 32 32 21
11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 2837 4187 5618 1316
Size: 1521 1350 1431 4528
Fragments arranged by size:
4528 1521 1431 1350
XbaI T'CTAG_A
Cuts at: 6166 6166
Size: 8830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4994 4994
Size: 8830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8830
XmaI C'CCGG_G
Cuts at: 1870 3868 5106 6155 1870
Size: 1998 1238 1049 4545
Fragments arranged by size:
4545 1998 1238 1049
XmnI GAAnn'nnTTC
Cuts at: 2546 2985 3336 3815 6959 2546
Size: 439 351 479 3144 4417
Fragments arranged by size:
4417 3144 479 439 351
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6638 276
Size: 53 83 186 936 5104 2468
Fragments arranged by size:
5104 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BsiWI Bsu36I EcoNI
EcoRV FseI KasI NarI NruI NsiI PacI PspXI
RsrII SanDI SfiI SfoI SrfI SwaI