Restriction map for
pFN21AB9001
(Circular) (Six-base) MAPSORT of: pFN21AB9001.seq Check: 6668 from: 1 to: 5983
pFN21AB9001 5983 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3123 4046 5430 5698 1632
Size: 1491 923 1384 268 1917
Fragments arranged by size:
1917 1491 1384 923 268
AfeI AGC'GCT
Cuts at: 2078 2078
Size: 5983
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 2567 4882 5859 1203
Size: 1364 2315 977 1327
Fragments arranged by size:
2315 1364 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 2896 4712 2896
Size: 1816 4167
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2643 2675 2722 2754 2643
Size: 32 47 32 5872
Fragments arranged by size:
5872 47 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3223 3726 5298 3223
Size: 503 1572 3908
Fragments arranged by size:
3908 1572 503
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 2744 3088 3292 3518 3670 5779 1493
Size: 1251 344 204 226 152 2109 1697
Fragments arranged by size:
2109 1697 1251 344 226 204 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 1870
Size: 73 1365 4545
Fragments arranged by size:
4545 1365 73
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2998 3304 4759 618
Size: 325 217 627 1211 306 1455 1842
Fragments arranged by size:
1842 1455 1211 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2113 2584 3302 1391
Size: 722 471 718 4072
Fragments arranged by size:
4072 722 718 471
BbeI G_GCGC'C
Cuts at: 3002 3002
Size: 5983
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2269 928
Size: 971 370 4642
Fragments arranged by size:
4642 971 370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BfrBI ATG'CAT
Cuts at: 2643 2643
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 2174 3400 2174
Size: 1226 4757
Bme1580I G_kGCm'C
Cuts at: 1790 4044 5200 1790
Size: 2254 1156 2573
Fragments arranged by size:
2573 2254 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3714 4672 442
Size: 416 53 83 399 24 2297 958 1753
Fragments arranged by size:
2297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3189 3189
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 4643 1239
Size: 1079 2325 2579
Fragments arranged by size:
2579 2325 1079
Bpu10I CC'TnA_GC
Cuts at: 2112 2112
Size: 5983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 4108 4973 5271 2057
Size: 2051 865 298 2769
Fragments arranged by size:
2769 2051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4646 882
Size: 617 3147 2219
Fragments arranged by size:
3147 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2946 2999 3790 4172
Size: 53 83 186 936 1413 53 791 382
Cuts at: 4172 4807 275
Size: 635 1451
Fragments arranged by size:
1451 1413 936 791 635 382 186 83
53 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2643 2673 3185 3215 1178
Size: 30 165 30 1240 30 512 30 3946
Fragments arranged by size:
3946 1240 512 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 3190 1186
Size: 1129 875 3979
Fragments arranged by size:
3979 1129 875
BseYI C'CCAG_C
Cuts at: 2845 2920 2983 5186 5682 2845
Size: 75 63 2203 496 3146
Fragments arranged by size:
3146 2203 496 75 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3165 1163
Size: 573 1429 3981
Fragments arranged by size:
3981 1429 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2862 4044 4129 5200 2862
Size: 1182 85 1071 3645
Fragments arranged by size:
3645 1182 1071 85
BsmI GAATG_Cn'
Cuts at: 3494 3587 3494
Size: 93 5890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2584 2862 3302 4044 4129 5200
Size: 399 323 471 278 440 742 85 1071
Cuts at: 5200 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 471 440 399 323 278
85
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 2232 2544 2748 3872 5602 2232
Size: 312 204 1124 1730 2613
Fragments arranged by size:
2613 1730 1124 312 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 3338 844
Size: 1559 935 3489
Fragments arranged by size:
3489 1559 935
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2438 2438
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 3313 3680 4065 4082 5534 5620 5632 5978
Size: 1341 367 385 17 1452 86 12 346
Cuts at: 5978 1972
Size: 1977
Fragments arranged by size:
1977 1452 1341 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2586 2586
Size: 5983
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5431
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 3156 3166 3499 4293 4313 3156
Size: 10 333 794 20 4826
Fragments arranged by size:
4826 794 333 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 2460 3287 2460
Size: 827 5156
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2520 3430
Size: 54 1425 50 275 18 330 360 910
Cuts at: 3430 4319 5948 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 910 889 360 330 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 2846 3913 1353
Size: 1478 15 1067 3423
Fragments arranged by size:
3423 1478 1067 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 4944 5090 387
Size: 1064 138 2981 374 146 1280
Fragments arranged by size:
2981 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 3123 4046 4643 5430 5698 1239
Size: 393 686 805 923 597 787 268 1524
Fragments arranged by size:
1524 923 805 787 686 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3263 3263
Size: 5983
EcoRI G'AATT_C
Cuts at: 2744 3088 3292 3670 2744
Size: 344 204 378 5057
Fragments arranged by size:
5057 378 344 204
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 2080 3002 5130 1678
Size: 402 922 2128 2531
Fragments arranged by size:
2531 2128 922 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 2615 2647 2882
Size: 32 135 32 800 32 211 32 235
Cuts at: 2882 2914 3185 3217 4624 4656 4698 4730 1373
Size: 32 271 32 1407 32 42 32 2626
Fragments arranged by size:
2626 1407 800 271 235 211 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2023 3327 3573 669
Size: 988 281 85 1304 246 3079
Fragments arranged by size:
3079 1304 988 281 246 85
HindIII A'AGCT_T
Cuts at: 748 2539 748
Size: 1791 4192
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KasI G'GCGC_C
Cuts at: 2998 2998
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3048 3582 3048
Size: 534 5449
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 5097 5281 1305
Size: 1704 2088 184 2007
Fragments arranged by size:
2088 2007 1704 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 2522 10
Size: 54 1425 343 330 360 3471
Fragments arranged by size:
3471 1425 360 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3941 4300 4459 518
Size: 648 2775 359 159 2042
Fragments arranged by size:
2775 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2849 3399 4076 5224 5469 1606
Size: 1243 550 677 1148 245 2120
Fragments arranged by size:
2120 1243 1148 677 550 245
NarI GG'CG_CC
Cuts at: 2999 2999
Size: 5983
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5431
NdeI CA'TA_TG
Cuts at: 387 2557 387
Size: 2170 3813
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NsiI A_TGCA'T
Cuts at: 2645 2645
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2204 3341 4886 1132
Size: 97 975 1137 1545 2229
Fragments arranged by size:
2229 1545 1137 975 97
PasI CC'CwG_GG
Cuts at: 1348 3268 1348
Size: 1920 4063
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3223 1108
Size: 2115 3868
PfoI T'CCnGG_A
Cuts at: 2505 2680 2505
Size: 175 5808
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2643 2675 4052 4084 5565 5597 2643
Size: 32 1377 32 1481 32 3029
Fragments arranged by size:
3029 1481 1377 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3263 3263
Size: 5983
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PstI C_TGCA'G
Cuts at: 830 3335 830
Size: 2505 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2035 3331 4466 5147 5338 826
Size: 219 990 1296 1135 681 191 1471
Fragments arranged by size:
1471 1296 1135 990 681 219 191
SfoI GGC'GCC
Cuts at: 3000 3000
Size: 5983
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2072 4087 4988 5250 820
Size: 197 926 129 2015 901 262 1553
Fragments arranged by size:
2015 1553 926 901 262 197 129
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 3422 513
Size: 552 2357 3074
Fragments arranged by size:
3074 2357 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4010 4027 4180 4365 4810 325
Size: 1255 2430 17 153 185 445 1498
Fragments arranged by size:
2430 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4229 5941 96
Size: 274 80 33 51 1443 2252 1712 138
Fragments arranged by size:
2252 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5943 5975 5943
Size: 32 5951
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2411 2411
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 2658 4112 2658
Size: 1454 4529
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI