Restriction map for
pFN21AB9000
(Circular) (Six-base) MAPSORT of: pFN21AB9000.seq Check: 2272 from: 1 to: 5959
pFN21AB9000 5959 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2386 2400 2386
Size: 14 5945
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2255 3769 278
Size: 53 83 186 936 719 1514 2468
Fragments arranged by size:
2468 1514 936 719 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 2702 3280 2702
Size: 578 5381
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 3048 4858 5835 1203
Size: 1845 1810 977 1327
Fragments arranged by size:
1845 1810 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2237 1184
Size: 1053 4906
AlwNI CAG_nnn'CTG
Cuts at: 2320 3702 5274 2320
Size: 1382 1572 3005
Fragments arranged by size:
3005 1572 1382
ApaI G_GGCC'C
Cuts at: 2037 2037
Size: 5959
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 3119 3239 3268 3494 3646 5755 1493
Size: 1626 120 29 226 152 2109 1697
Fragments arranged by size:
2109 1697 1626 226 152 120 29
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2108 2853 3284 1870
Size: 73 95 70 745 431 4545
Fragments arranged by size:
4545 745 431 95 73 70
BamHI G'GATC_C
Cuts at: 2953 3289 3656 2953
Size: 336 367 5256
Fragments arranged by size:
5256 367 336
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 2702 2917 3280 4735
Size: 325 217 627 896 19 215 363 1455
Cuts at: 4735 618
Size: 1842
Fragments arranged by size:
1842 1455 896 627 363 325 217 215
19
BanII G_rGCy'C
Cuts at: 1391 2037 3278 1391
Size: 646 1241 4072
Fragments arranged by size:
4072 1241 646
BbeI G_GCGC'C
Cuts at: 2687 2921 2687
Size: 234 5725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2560 2759 3160 928
Size: 971 661 199 401 3727
Fragments arranged by size:
3727 971 661 401 199
BbvCI CC'TCA_GC
Cuts at: 2282 2282
Size: 5959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2119 2153 4150 4184 1903
Size: 34 50 34 98 34 1997 34 3678
Fragments arranged by size:
3678 1997 98 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2177 2297 4570
Size: 107 122 71 631 605 505 120 2273
Cuts at: 4570 136
Size: 1525
Fragments arranged by size:
2273 1525 631 605 505 122 120 107
71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 2534 2546 3376 2534
Size: 12 830 5117
Fragments arranged by size:
5117 830 12
Bme1580I G_kGCm'C
Cuts at: 1790 2037 4020 5176 1790
Size: 247 1983 1156 2573
Fragments arranged by size:
2573 1983 1156 247
BmgBI CAC'GTC
Cuts at: 1318 2137 1318
Size: 819 5140
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3020 3690 4648
Size: 416 53 83 399 24 1603 670 958
Cuts at: 4648 442
Size: 1753
Fragments arranged by size:
1753 1603 958 670 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2183 4619 1239
Size: 793 151 2436 2579
Fragments arranged by size:
2579 2436 793 151
Bpu10I CC'TnA_GC
Cuts at: 2282 2282
Size: 5959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 4084 4949 5247 2459
Size: 1625 865 298 3171
Fragments arranged by size:
3171 1625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2653 3750 493
Size: 711 1449 1097 2702
Fragments arranged by size:
2702 1449 1097 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2240 2252 2684 2759
Size: 53 83 186 936 707 12 432 75
Cuts at: 2759 2918 3766 4148 4783 275
Size: 159 848 382 635 1451
Fragments arranged by size:
1451 936 848 707 635 432 382 186
159 83 75 53 12
BsaWI w'CCGG_w
Cuts at: 2235 4392 4790 5064 5211 5622 2235
Size: 2157 398 274 147 411 2572
Fragments arranged by size:
2572 2157 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 1186
Size: 976 4983
BseYI C'CCAG_C
Cuts at: 2203 2334 2613 2913 5162 5658 2203
Size: 131 279 300 2249 496 2504
Fragments arranged by size:
2504 2249 496 300 279 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 2799 1163
Size: 573 480 583 4323
Fragments arranged by size:
4323 583 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2449 2523 3409 4170 5198 1540
Size: 275 186 448 74 886 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 448 275 186 74
BsiHKAI G_wGCw'C
Cuts at: 2552 4020 4105 5176 2552
Size: 1468 85 1071 3335
Fragments arranged by size:
3335 1468 1071 85
BsmI GAATG_Cn'
Cuts at: 2306 3470 3563 2306
Size: 1164 93 4702
Fragments arranged by size:
4702 1164 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2015 2015
Size: 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2552 3278 4020 4105 5176 1391
Size: 399 247 515 726 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 726 515 399 247 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2436 2511 3848 5578 2436
Size: 75 1337 1730 2817
Fragments arranged by size:
2817 1730 1337 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 2400 2714 3314 844
Size: 1542 14 314 600 3489
Fragments arranged by size:
3489 1542 600 314 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2473 2502 3846 1394
Size: 429 650 29 1344 3507
Fragments arranged by size:
3507 1344 650 429 29
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2072 4603 4790 1700
Size: 110 208 54 2531 187 2869
Fragments arranged by size:
2869 2531 208 187 110 54
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 2495 1925
Size: 570 5389
BssSI C'ACGA_G
Cuts at: 2643 4019 5031 2643
Size: 1376 1012 3571
Fragments arranged by size:
3571 1376 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2862 2953 3289 3656 4041 4058 5510 5596
Size: 890 91 336 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 890 385 367 346 336 91
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2054 2301 2356 2688 2746 513
Size: 552 989 247 55 332 58 3726
Fragments arranged by size:
3726 989 552 332 247 58 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2440 487
Size: 683 56 1214 4006
Fragments arranged by size:
4006 1214 683 56
BtsI GCAGTG_nn'
Cuts at: 3000 3475 4269 4289 3000
Size: 475 794 20 4670
Fragments arranged by size:
4670 794 475 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DraIII CAC_nnn'GTG
Cuts at: 2828 2828
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2154 2196
Size: 54 1425 50 275 18 237 87 42
Cuts at: 2196 2289 2298 2520 3061 3406 4295 5924 8
Size: 93 9 222 541 345 889 1629 43
Fragments arranged by size:
1629 1425 889 541 345 275 237 222
93 87 54 50 43 42 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2520 3406 1537
Size: 275 708 886 4090
Fragments arranged by size:
4090 886 708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 3889 1353
Size: 920 1616 3423
Fragments arranged by size:
3423 1616 920
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2005 4546 4920 5066 387
Size: 1064 138 416 2541 374 146 1280
Fragments arranged by size:
2541 1280 1064 416 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2183 4022 4619 5406 5674 1239
Size: 393 400 151 1839 597 787 268 1524
Fragments arranged by size:
1839 1524 787 597 400 393 268 151
EcoNI CCTnn'n_nnAGG
Cuts at: 2111 2111
Size: 5959
EcoO109I rG'GnC_Cy
Cuts at: 2033 2034 2033
Size: 1 5958
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3177 3177
Size: 5959
FspI TGC'GCA
Cuts at: 2141 4465 2141
Size: 2324 3635
HaeII r_GCGC'y
Cuts at: 1678 2079 2687 2921 5106 1678
Size: 401 608 234 2185 2531
Fragments arranged by size:
2531 2185 608 401 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2945 2977 4600 4632 4674
Size: 32 135 32 1373 32 1623 32 42
Cuts at: 4674 4706 1373
Size: 32 2626
Fragments arranged by size:
2626 1623 1373 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3088 748
Size: 2340 3619
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2683 2917 2683
Size: 234 5725
KpnI G_GTAC'C
Cuts at: 2706 3284 2706
Size: 578 5381
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2299 5073 5257 1305
Size: 994 2774 184 2007
Fragments arranged by size:
2774 2007 994 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 2156 3063 10
Size: 54 1425 343 237 87 907 2906
Fragments arranged by size:
2906 1425 907 343 237 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2678 2936 3917 4276 4435 518
Size: 648 1293 219 258 981 359 159 2042
Fragments arranged by size:
2042 1293 981 648 359 258 219 159
MspA1I CmG'CkG
Cuts at: 1606 2358 2748 3375 4052 5200 5445 1606
Size: 752 390 627 677 1148 245 2120
Fragments arranged by size:
2120 1148 752 677 627 390 245
NarI GG'CG_CC
Cuts at: 2684 2918 2684
Size: 234 5725
NcoI C'CATG_G
Cuts at: 513 1065 2054 513
Size: 552 989 4418
Fragments arranged by size:
4418 989 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 2520 3406 2520
Size: 886 5073
NspI r_CATG'y
Cuts at: 1132 1229 2263 2410 2428 3052 3317 4862 1132
Size: 97 1034 147 18 624 265 1545 2229
Fragments arranged by size:
2229 1545 1034 624 265 147 97 18
PasI CC'CwG_GG
Cuts at: 1348 2634 1348
Size: 1286 4673
PciI A'CATG_T
Cuts at: 3048 4858 3048
Size: 1810 4149
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2055 1108
Size: 947 5012
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2033 2033
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2397 3311 830
Size: 1567 914 3478
Fragments arranged by size:
3478 1567 914
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacII CC_GC'GG
Cuts at: 2359 2749 2359
Size: 390 5569
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 2397 3311 2397
Size: 914 5045
ScaI AGT'ACT
Cuts at: 2561 4207 5919 2561
Size: 1646 1712 2601
Fragments arranged by size:
2601 1712 1646
SfcI C'TryA_G
Cuts at: 826 1045 2393 3307 4442 5123 5314 826
Size: 219 1348 914 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 914 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2297 2297
Size: 5959
SfoI GGC'GCC
Cuts at: 2685 2919 2685
Size: 234 5725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 2072 1700
Size: 372 5587
SmaI CCC'GGG
Cuts at: 1872 2855 3286 1872
Size: 983 431 4545
Fragments arranged by size:
4545 983 431
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 4063 4964 5226 820
Size: 197 926 531 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 531 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 2263 3317 1132
Size: 1131 1054 3774
Fragments arranged by size:
3774 1131 1054
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2054 2598 3398 513
Size: 552 989 544 800 3074
Fragments arranged by size:
3074 989 800 552 544
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2518 2987 3986 4003 4156 4341 4786
Size: 1255 938 469 999 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1498 1255 999 938 469 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2559 4205 5917
Size: 274 80 33 51 1443 582 1646 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1712 1646 1443 582 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 2758 1316
Size: 1442 4517
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 2853 3284 1870
Size: 983 431 4545
Fragments arranged by size:
4545 983 431
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2253 3767 276
Size: 53 83 186 936 719 1514 2468
Fragments arranged by size:
2468 1514 936 719 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI
SwaI XcmI