Restriction map for
pFN21AB8998
(Circular) (Six-base) MAPSORT of: pFN21AB8998.seq Check: 2836 from: 1 to: 5941
pFN21AB8998 5941 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2266 2648 4004 5388 5656 1632
Size: 634 382 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1356 634 382 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 2650 3684 5256 2650
Size: 1034 1572 3335
Fragments arranged by size:
3335 1572 1034
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2967 3266 1870
Size: 73 1024 299 4545
Fragments arranged by size:
4545 1024 299 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2566 2957 3262 4717 618
Size: 325 217 627 779 391 305 1455 1842
Fragments arranged by size:
1842 1455 779 627 391 325 305 217
BanII G_rGCy'C
Cuts at: 1391 2021 2276 2727 3159 3174 3195 3260 1391
Size: 630 255 451 432 15 21 65 4072
Fragments arranged by size:
4072 630 451 432 255 65 21 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4132 4166 1903
Size: 34 50 34 2111 34 3678
Fragments arranged by size:
3678 2111 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2888 3833 5043 2888
Size: 945 1210 3786
Fragments arranged by size:
3786 1210 945
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4552 136
Size: 107 122 71 631 605 2880 1525
Fragments arranged by size:
2880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2571 2668 2968 3118 3188 4002 5158 1790
Size: 781 97 300 150 70 814 1156 2573
Fragments arranged by size:
2573 1156 814 781 300 150 97 70
BmgBI CAC'GTC
Cuts at: 1318 2923 3151 1318
Size: 1605 228 4108
Fragments arranged by size:
4108 1605 228
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2664 2664
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4601 1239
Size: 3362 2579
Bpu10I CC'TnA_GC
Cuts at: 2367 3066 3161 2367
Size: 699 95 5147
Fragments arranged by size:
5147 699 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3158 4066 4931 5229 3158
Size: 908 865 298 3870
Fragments arranged by size:
3870 908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3748 4130 4765 275
Size: 53 83 186 936 2215 382 635 1451
Fragments arranged by size:
2215 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2334 4374 4772 5046 5193 5604 2334
Size: 2040 398 274 147 411 2671
Fragments arranged by size:
2671 2040 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2988 3018 3219 3225 3249
Size: 30 165 30 1585 30 201 6 24
Cuts at: 3249 3255 1178
Size: 6 3864
Fragments arranged by size:
3864 1585 201 165 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2011 1186
Size: 825 5116
BseYI C'CCAG_C
Cuts at: 2088 2388 2584 5144 5640 2088
Size: 300 196 2560 496 2389
Fragments arranged by size:
2560 2389 496 300 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5368
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2157 3391 4152 5180 1540
Size: 275 186 156 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186 156
BsiHKAI G_wGCw'C
Cuts at: 2178 2276 3058 3159 3174 4002 4087 5158 2178
Size: 98 782 101 15 828 85 1071 2961
Fragments arranged by size:
2961 1071 828 782 101 98 85 15
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 3020 3020
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2178 2276 2571 2668 2727 2968
Size: 399 231 157 98 295 97 59 241
Cuts at: 2968 3058 3118 3159 3174 3188 3195 3260 4002
Size: 90 60 41 15 14 7 65 742
Cuts at: 4002 4087 5158 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 295 241 231 157
98 97 90 85 65 60 59 41
15 14 7
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2992 3296 844
Size: 1277 871 304 3489
Fragments arranged by size:
3489 1277 871 304
BsrBI CCG'CTC
Cuts at: 1394 1823 2332 2662 3070 3828 1394
Size: 429 509 330 408 758 3507
Fragments arranged by size:
3507 758 509 429 408 330
BsrDI GCAATG_nn'
Cuts at: 65 1248 2049 2080 2241 2313 2376 4436 4610
Size: 1183 801 31 161 72 63 2060 174
Cuts at: 4610 65
Size: 1396
Fragments arranged by size:
2060 1396 1183 801 174 161 72 63
31
BsrFI r'CCGG_y
Cuts at: 1700 1810 2354 4585 4772 1700
Size: 110 544 2231 187 2869
Fragments arranged by size:
2869 2231 544 187 110
BsrGI T'GTAC_A
Cuts at: 96 2028 96
Size: 1932 4009
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 2359 4001 5013 2359
Size: 1642 1012 3287
Fragments arranged by size:
3287 1642 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 2350 1184
Size: 1166 4775
BstXI CCAn_nnnn'nTGG
Cuts at: 2707 2719 3039 2707
Size: 12 320 5609
Fragments arranged by size:
5609 320 12
BstYI r'GATC_y
Cuts at: 1972 2601 3271 3638 4023 4040 5492 5578 5590
Size: 629 670 367 385 17 1452 86 12
Cuts at: 5590 5936 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 670 629 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2927 2927
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2227 513
Size: 552 1162 4227
Fragments arranged by size:
4227 1162 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2146 2281 487
Size: 683 56 920 135 4147
Fragments arranged by size:
4147 920 683 135 56
BtsI GCAGTG_nn'
Cuts at: 2172 2675 2995 3058 3457 4251 4271 2172
Size: 503 320 63 399 794 20 3842
Fragments arranged by size:
3842 794 503 399 320 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2061 2154
Size: 54 1425 50 275 18 216 15 93
Cuts at: 2154 2292 2709 2820 3022 3388 4277 5906 8
Size: 138 417 111 202 366 889 1629 43
Fragments arranged by size:
1629 1425 889 417 366 275 216 202
138 111 93 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2154 3388 1537
Size: 275 342 1234 4090
Fragments arranged by size:
4090 1234 342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 3871 1353
Size: 890 1628 3423
Fragments arranged by size:
3423 1628 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2879 4528 4902 5048 387
Size: 1064 138 1290 1649 374 146 1280
Fragments arranged by size:
1649 1290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2274 3157 3172 2274
Size: 883 15 5043
Fragments arranged by size:
5043 883 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2266 2648 4004 4601 5388 5656 1239
Size: 393 634 382 1356 597 787 268 1524
Fragments arranged by size:
1524 1356 787 634 597 393 382 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3218 3218
Size: 5941
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 5088 1678
Size: 3410 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2988 3020 4582 4614 4656
Size: 32 135 32 1416 32 1562 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 1562 1416 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2411 5055 5239 1305
Size: 1106 2644 184 2007
Fragments arranged by size:
2644 2007 1106 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 2063 2294 2711 2822
Size: 54 1425 343 216 15 231 417 111
Cuts at: 2822 10
Size: 3129
Fragments arranged by size:
3129 1425 417 343 231 216 111 54
15
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2054 2165 2717 2988 3899 4258
Size: 648 870 18 111 552 271 911 359
Cuts at: 4258 4417 518
Size: 159 2042
Fragments arranged by size:
2042 911 870 648 552 359 271 159
111 18
MspA1I CmG'CkG
Cuts at: 1606 2626 2954 3357 4034 5182 5427 1606
Size: 1020 328 403 677 1148 245 2120
Fragments arranged by size:
2120 1148 1020 677 403 328 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5389
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2473 3091 3127 1348
Size: 1125 618 36 4162
Fragments arranged by size:
4162 1125 618 36
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2388 1108
Size: 1280 4661
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2118 2682 2792 3293 830
Size: 1288 564 110 501 3478
Fragments arranged by size:
3478 1288 564 501 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2626 2954 2626
Size: 328 5613
SacI G_AGCT'C
Cuts at: 2276 3159 3174 2276
Size: 883 15 5043
Fragments arranged by size:
5043 883 15
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2114 2678 2788 3289 4424 5105 5296
Size: 219 1069 564 110 501 1135 681 191
Cuts at: 5296 826
Size: 1471
Fragments arranged by size:
1471 1135 1069 681 564 501 219 191
110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2969 3268 1872
Size: 1097 299 4545
Fragments arranged by size:
4545 1097 299
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3173 4045 4946 5208 820
Size: 197 926 1230 872 901 262 1553
Fragments arranged by size:
1553 1230 926 901 872 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 3380 513
Size: 552 2315 3074
Fragments arranged by size:
3074 2315 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 2380 2576 3968 3985 4138 4323
Size: 1255 579 221 196 1392 17 153 185
Cuts at: 4323 4768 325
Size: 445 1498
Fragments arranged by size:
1498 1392 1255 579 445 221 196 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2028 2538 4187
Size: 274 80 33 51 1443 51 510 1649
Cuts at: 4187 5899 96
Size: 1712 138
Fragments arranged by size:
1712 1649 1443 510 274 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3219 3251 5901 5933 3219
Size: 32 2650 32 3227
Fragments arranged by size:
3227 2650 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2056 2134 2530 2011
Size: 45 78 396 5422
Fragments arranged by size:
5422 396 78 45
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2967 3266 1870
Size: 1097 299 4545
Fragments arranged by size:
4545 1097 299
XmnI GAAnn'nnTTC
Cuts at: 2858 4070 2858
Size: 1212 4729
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstZ17I DraIII EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI