Restriction map for
pFN21AB8994
(Circular) (Six-base) MAPSORT of: pFN21AB8994.seq Check: 1821 from: 1 to: 5692
pFN21AB8994 5692 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2158 2158
Size: 5692
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 2976 3035 1937
Size: 328 711 59 4594
Fragments arranged by size:
4594 711 328 59
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 2991 3819 4192 1563
Size: 1428 828 373 3063
Fragments arranged by size:
3063 1428 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3755 5139 5407 1632
Size: 2123 1384 268 1917
Fragments arranged by size:
2123 1917 1384 268
AfeI AGC'GCT
Cuts at: 2330 2858 2330
Size: 528 5164
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2905 3435 5007 2905
Size: 530 1572 3590
Fragments arranged by size:
3590 1572 530
ApaI G_GGCC'C
Cuts at: 2653 2718 2653
Size: 65 5627
ApaLI G'TGCA_C
Cuts at: 2318 3749 4905 2318
Size: 1431 1156 3105
Fragments arranged by size:
3105 1431 1156
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2646 2922 3017 1870
Size: 73 703 276 95 4545
Fragments arranged by size:
4545 703 276 95 73
AvrII C'CTAG_G
Cuts at: 2803 2803
Size: 5692
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2456 2892 3013 4468
Size: 325 217 627 242 427 436 121 1455
Cuts at: 4468 618
Size: 1842
Fragments arranged by size:
1842 1455 627 436 427 325 242 217
121
BanII G_rGCy'C
Cuts at: 1391 2064 2520 2634 2653 2718 3011 1391
Size: 673 456 114 19 65 293 4072
Fragments arranged by size:
4072 673 456 293 114 65 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4721
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2773 4303 136
Size: 107 122 71 631 605 1101 1530 1525
Fragments arranged by size:
1530 1525 1101 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2322 2653 2677 2718 2832 3753 4909
Size: 244 288 331 24 41 114 921 1156
Cuts at: 4909 1790
Size: 2573
Fragments arranged by size:
2573 1156 921 331 288 244 114 41
24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2780 3423 4381
Size: 416 53 83 399 24 1363 643 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1363 958 643 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 2627 2680 2703 2893 4352 1239
Size: 1100 288 53 23 190 1459 2579
Fragments arranged by size:
2579 1459 1100 288 190 53 23
Bpu10I CC'TnA_GC
Cuts at: 2168 2168
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2722 3483 493
Size: 711 1518 761 2702
Fragments arranged by size:
2702 1518 761 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3499 3881 4516
Size: 53 83 186 936 476 1490 382 635
Cuts at: 4516 275
Size: 1451
Fragments arranged by size:
1490 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 1178
Size: 30 165 30 1050 30 4387
Fragments arranged by size:
4387 1050 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2378 2726 2786 1186
Size: 1063 129 348 60 4092
Fragments arranged by size:
4092 1063 348 129 60
BseYI C'CCAG_C
Cuts at: 2033 2183 2485 2709 2845 2939 4895 5391 2033
Size: 150 302 224 136 94 1956 496 2334
Fragments arranged by size:
2334 1956 496 302 224 150 136 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2838 1163
Size: 573 1102 4017
Fragments arranged by size:
4017 1102 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 3753 3838 4909 2322
Size: 1431 85 1071 3105
Fragments arranged by size:
3105 1431 1071 85
BsmI GAATG_Cn'
Cuts at: 2370 3203 3296 2370
Size: 833 93 4766
Fragments arranged by size:
4766 833 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2064 2322 2520 2634 2653 2677
Size: 399 244 30 258 198 114 19 24
Cuts at: 2677 2718 2832 3011 3753 3838 4909 1391
Size: 41 114 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 258 244 198 179
114 114 85 41 30 24 19
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 2158 3047 844
Size: 1265 49 889 3489
Fragments arranged by size:
3489 1265 889 49
BsrBI CCG'CTC
Cuts at: 1394 1823 2429 2611 3579 1394
Size: 429 606 182 968 3507
Fragments arranged by size:
3507 968 606 429 182
BsrDI GCAATG_nn'
Cuts at: 65 1248 2204 2210 2943 4187 4361 65
Size: 1183 956 6 733 1244 174 1396
Fragments arranged by size:
1396 1244 1183 956 733 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 2601 4336 4523 1700
Size: 110 791 1735 187 2869
Fragments arranged by size:
2869 1735 791 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2223 2363 2845 2223
Size: 140 482 5070
Fragments arranged by size:
5070 482 140
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2345 2345
Size: 5692
BtgI C'CryG_G
Cuts at: 513 1065 2532 2838 513
Size: 552 1467 306 3367
Fragments arranged by size:
3367 1467 552 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2104 2640 487
Size: 683 56 878 536 3539
Fragments arranged by size:
3539 878 683 536 56
BtsI GCAGTG_nn'
Cuts at: 2571 2622 2729 2832 2977 3208 4002 4022 2571
Size: 51 107 103 145 231 794 20 4241
Fragments arranged by size:
4241 794 231 145 107 103 51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2788 4699 809
Size: 507 1472 1911 1802
Fragments arranged by size:
1911 1802 1472 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2568 2724 3139
Size: 54 1425 50 275 18 738 156 415
Cuts at: 3139 4028 5657 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 738 415 275 156 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2702 2908 3622 1353
Size: 1349 206 714 3423
Fragments arranged by size:
3423 1349 714 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2626 4279 4653 4799 387
Size: 1064 138 1037 1653 374 146 1280
Fragments arranged by size:
1653 1280 1064 1037 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2627 2680 2703 2893 3755 4352
Size: 393 707 288 53 23 190 862 597
Cuts at: 4352 5139 5407 1239
Size: 787 268 1524
Fragments arranged by size:
1524 862 787 707 597 393 288 268
190 53 23
EcoO109I rG'GnC_Cy
Cuts at: 2650 2714 2715 2774 2650
Size: 64 1 59 5568
Fragments arranged by size:
5568 64 59 1
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 2141 4198 2141
Size: 2057 3635
HaeII r_GCGC'y
Cuts at: 1678 2332 2764 2860 4839 1678
Size: 654 432 96 1979 2531
Fragments arranged by size:
2531 1979 654 432 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2618 2650 4333 4365 4407
Size: 32 135 32 1046 32 1683 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1683 1046 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2977 3036 3282 669
Size: 988 281 1039 59 246 3079
Fragments arranged by size:
3079 1039 988 281 246 59
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 2726 10
Size: 54 1425 343 738 156 2976
Fragments arranged by size:
2976 1425 738 343 156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3650 4009 4168 518
Size: 648 2484 359 159 2042
Fragments arranged by size:
2484 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2587 2822 3108 3785 4933 5178 1606
Size: 981 235 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 677 286 245 235
NaeI GCC'GGC
Cuts at: 2603 2603
Size: 5692
NcoI C'CATG_G
Cuts at: 513 1065 2838 513
Size: 552 1773 3367
Fragments arranged by size:
3367 1773 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NgoMIV G'CCGG_C
Cuts at: 2601 2601
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 3050 4595 1132
Size: 97 1821 1545 2229
Fragments arranged by size:
2229 1821 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2113 2363 1348
Size: 765 250 4677
Fragments arranged by size:
4677 765 250
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2153 1108
Size: 1045 4647
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2649 2714 2649
Size: 65 5627
PstI C_TGCA'G
Cuts at: 830 2468 2592 2901 3044 830
Size: 1638 124 309 143 3478
Fragments arranged by size:
3478 1638 309 143 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2587 2587
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 2975 3034 1936
Size: 1039 59 4594
Fragments arranged by size:
4594 1039 59
SapI GCTCTTCn'nnn_
Cuts at: 2702 2908 2702
Size: 206 5486
SbfI CC_TGCA'GG
Cuts at: 2901 3044 2901
Size: 143 5549
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2464 2588 2897 3040 4175 4856 5047
Size: 219 1419 124 309 143 1135 681 191
Cuts at: 5047 826
Size: 1471
Fragments arranged by size:
1471 1419 1135 681 309 219 191 143
124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 2648 2924 3019 1872
Size: 776 276 95 4545
Fragments arranged by size:
4545 776 276 95
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SrfI GCCC'GGGC
Cuts at: 2648 2648
Size: 5692
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2046 2803 2838 3131 513
Size: 552 981 757 35 293 3074
Fragments arranged by size:
3074 981 757 552 293 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2525 2970 3719 3736 3889 4074 4519
Size: 1255 945 445 749 17 153 185 445
Cuts at: 4519 325
Size: 1498
Fragments arranged by size:
1498 1255 945 749 445 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2440 2472 5652 5684 2440
Size: 32 3180 32 2448
Fragments arranged by size:
3180 2448 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2311 2450 2053
Size: 258 139 5295
Fragments arranged by size:
5295 258 139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 2646 2922 3017 1870
Size: 776 276 95 4545
Fragments arranged by size:
4545 776 276 95
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SwaI