Restriction map for
pFN21AB8988
(Circular) (Six-base) MAPSORT of: pFN21AB8988.seq Check: 1855 from: 1 to: 5869
pFN21AB8988 5869 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2721 2721
Size: 5869
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3212 1937
Size: 1275 4594
Acc65I G'GTAC_C
Cuts at: 3190 3190
Size: 5869
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3932 5316 5584 1632
Size: 2300 1384 268 1917
Fragments arranged by size:
2300 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 2589 4768 5745 1203
Size: 1386 2179 977 1327
Fragments arranged by size:
2179 1386 1327 977
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2531 1184
Size: 1347 4522
AlwNI CAG_nnn'CTG
Cuts at: 3612 5184 3612
Size: 1572 4297
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 2996 3178 3404 3556 5665 1493
Size: 1503 182 226 152 2109 1697
Fragments arranged by size:
2109 1697 1503 226 182 152
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2045 2110 2395 2421 2575 3194
Size: 73 93 9 65 285 26 154 619
Cuts at: 3194 1870
Size: 4545
Fragments arranged by size:
4545 619 285 154 93 73 65 26
9
BamHI G'GATC_C
Cuts at: 3199 3566 3199
Size: 367 5502
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2570 2721 3190 4645 618
Size: 325 217 627 783 151 469 1455 1842
Fragments arranged by size:
1842 1455 783 627 469 325 217 151
BanII G_rGCy'C
Cuts at: 1391 2126 2229 2414 2780 3188 1391
Size: 735 103 185 366 408 4072
Fragments arranged by size:
4072 735 408 366 185 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2980 3014 4060 4094 1903
Size: 34 1043 34 1046 34 3678
Fragments arranged by size:
3678 1046 1043 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2147 3761 4971 2147
Size: 1614 1210 3045
Fragments arranged by size:
3045 1614 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2529 3044 4480
Size: 107 122 71 631 605 857 515 1436
Cuts at: 4480 136
Size: 1525
Fragments arranged by size:
1525 1436 857 631 605 515 122 107
71
BglII A'GATC_T
Cuts at: 5864 5864
Size: 5869
BlpI GC'TnA_GC
Cuts at: 2228 3286 2228
Size: 1058 4811
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2724 3930 5086 1790
Size: 264 670 1206 1156 2573
Fragments arranged by size:
2573 1206 1156 670 264
BmgBI CAC'GTC
Cuts at: 1318 2638 1318
Size: 1320 4549
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3600 4558 442
Size: 416 53 83 399 24 2183 958 1753
Fragments arranged by size:
2183 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 4529 1239
Size: 1424 1866 2579
Fragments arranged by size:
2579 1866 1424
Bpu10I CC'TnA_GC
Cuts at: 2925 2925
Size: 5869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3994 4859 5157 3994
Size: 865 298 4706
Fragments arranged by size:
4706 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 3079 3098 4532 882
Size: 617 549 1031 19 1434 2219
Fragments arranged by size:
2219 1434 1031 617 549 19
BsaAI yAC'GTr
Cuts at: 493 1204 2287 2592 3144 3660 493
Size: 711 1083 305 552 516 2702
Fragments arranged by size:
2702 1083 711 552 516 305
BsaBI GATnn'nnATC
Cuts at: 5863 5863
Size: 5869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2805 2943 3676
Size: 53 83 186 936 531 741 138 733
Cuts at: 3676 4058 4693 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 741 733 635 531 382 186
138 83 53
BsaWI w'CCGG_w
Cuts at: 2734 3070 4302 4700 4974 5121 5532 2734
Size: 336 1232 398 274 147 411 3071
Fragments arranged by size:
3071 1232 411 398 336 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 3017 3047 1178
Size: 30 165 30 705 30 879 30 4000
Fragments arranged by size:
4000 879 705 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2656 2696 1186
Size: 1470 40 4359
Fragments arranged by size:
4359 1470 40
BseYI C'CCAG_C
Cuts at: 2017 2239 2470 2519 5072 5568 2017
Size: 222 231 49 2553 496 2318
Fragments arranged by size:
2553 2318 496 231 222 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2219 1163
Size: 573 462 21 4813
Fragments arranged by size:
4813 573 462 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2097 2351 3319 4080 5108 1540
Size: 275 186 96 254 968 761 1028 2301
Fragments arranged by size:
2301 1028 968 761 275 254 186 96
BsiHKAI G_wGCw'C
Cuts at: 2229 2414 2931 3930 4015 5086 2229
Size: 185 517 999 85 1071 3012
Fragments arranged by size:
3012 1071 999 517 185 85
BsiWI C'GTAC_G
Cuts at: 2283 2598 2283
Size: 315 5554
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
BsmBI CGTCTCn'nnnn_
Cuts at: 2812 2812
Size: 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2126 2229 2414 2724 2780 2931
Size: 399 264 72 103 185 310 56 151
Cuts at: 2931 3188 3930 4015 5086 1391
Size: 257 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 310 264 257 185
151 103 85 72 56
BspEI T'CCGG_A
Cuts at: 5532 5532
Size: 5869
BspHI T'CATG_A
Cuts at: 3758 5488 3758
Size: 1730 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 2721 3224 844
Size: 1376 501 503 3489
Fragments arranged by size:
3489 1376 503 501
BsrBI CCG'CTC
Cuts at: 1394 1823 2302 3756 1394
Size: 429 479 1454 3507
Fragments arranged by size:
3507 1454 479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2998 4364 4538 65
Size: 1183 1750 1366 174 1396
Fragments arranged by size:
1750 1396 1366 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 2346 2548 4513 4700 1700
Size: 110 282 254 202 1965 187 2869
Fragments arranged by size:
2869 1965 282 254 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5869
BssSI C'ACGA_G
Cuts at: 2540 3929 4941 2540
Size: 1389 1012 3468
Fragments arranged by size:
3468 1389 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 794
Size: 1526 4343
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstYI r'GATC_y
Cuts at: 1972 3199 3566 3951 3968 5420 5506 5518 5864
Size: 1227 367 385 17 1452 86 12 346
Cuts at: 5864 1972
Size: 1977
Fragments arranged by size:
1977 1452 1227 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3048 3135 513
Size: 552 939 1044 87 3247
Fragments arranged by size:
3247 1044 939 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5130
Fragments arranged by size:
5130 683 56
BtsI GCAGTG_nn'
Cuts at: 2202 2508 2587 3385 4179 4199 2202
Size: 306 79 798 794 20 3872
Fragments arranged by size:
3872 798 794 306 79 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 809
Size: 507 3560 1802
Fragments arranged by size:
3560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2348 2689
Size: 54 1425 50 275 18 264 254 341
Cuts at: 2689 2791 2862 3022 3138 3316 4205 5834 8
Size: 102 71 160 116 178 889 1629 43
Fragments arranged by size:
1629 1425 889 341 275 264 254 178
160 116 102 71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2094 2348 3316 1537
Size: 275 282 254 968 4090
Fragments arranged by size:
4090 968 282 275 254
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2441 3799 1353
Size: 917 171 1358 3423
Fragments arranged by size:
3423 1358 917 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2312 3025 4456 4830 4976 387
Size: 1064 138 723 713 1431 374 146 1280
Fragments arranged by size:
1431 1280 1064 723 713 374 146 138
EcoICRI GAG'CTC
Cuts at: 2227 2412 2227
Size: 185 5684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2663 3932 4529 5316 5584 1239
Size: 393 1031 1269 597 787 268 1524
Fragments arranged by size:
1524 1269 1031 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3095 3095
Size: 5869
EcoRI G'AATT_C
Cuts at: 2996 3178 3556 2996
Size: 182 378 5309
Fragments arranged by size:
5309 378 182
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 2261 2471 5016 1678
Size: 583 210 2545 2531
Fragments arranged by size:
2545 2531 583 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2108 2140 4510
Size: 32 135 32 497 32 7 32 2370
Cuts at: 4510 4542 4584 4616 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2370 497 135 42 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3213 3459 669
Size: 988 281 1275 246 3079
Fragments arranged by size:
3079 1275 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 3194 3194
Size: 5869
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2055 2910 4983 5167 1305
Size: 750 855 2073 184 2007
Fragments arranged by size:
2073 2007 855 750 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2691 2793 3024 10
Size: 54 1425 343 859 102 231 2855
Fragments arranged by size:
2855 1425 859 343 231 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3827 4186 4345 518
Size: 648 2661 359 159 2042
Fragments arranged by size:
2661 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3285 3962 5110 5355 1606
Size: 1679 677 1148 245 2120
Fragments arranged by size:
2120 1679 1148 677 245
NaeI GCC'GGC
Cuts at: 2094 2550 2094
Size: 456 5413
NcoI C'CATG_G
Cuts at: 513 1065 2004 3048 513
Size: 552 939 1044 3334
Fragments arranged by size:
3334 1044 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NgoMIV G'CCGG_C
Cuts at: 2092 2548 2092
Size: 456 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 2323 3068 3227 4772 1132
Size: 97 1094 745 159 1545 2229
Fragments arranged by size:
2229 1545 1094 745 159 97
PasI CC'CwG_GG
Cuts at: 1348 2773 1348
Size: 1425 4444
PciI A'CATG_T
Cuts at: 4768 4768
Size: 5869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2773 1108
Size: 1665 4204
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3095 3095
Size: 5869
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PstI C_TGCA'G
Cuts at: 830 2316 3221 830
Size: 1486 905 3478
Fragments arranged by size:
3478 1486 905
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
SacI G_AGCT'C
Cuts at: 2229 2414 2229
Size: 185 5684
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SanDI GG'GwC_CC
Cuts at: 3095 3095
Size: 5869
SbfI CC_TGCA'GG
Cuts at: 3221 3221
Size: 5869
ScaI AGT'ACT
Cuts at: 2498 4117 5829 2498
Size: 1619 1712 2538
Fragments arranged by size:
2538 1712 1619
SfcI C'TryA_G
Cuts at: 826 1045 2312 2761 3157 3217 4352 5033 5224
Size: 219 1267 449 396 60 1135 681 191
Cuts at: 5224 826
Size: 1471
Fragments arranged by size:
1471 1267 1135 681 449 396 219 191
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 2112 2397 2423 3196 1872
Size: 240 285 26 773 4545
Fragments arranged by size:
4545 773 285 240 26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 3973 4874 5136 820
Size: 197 926 102 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 3227 1132
Size: 2095 3774
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5869
StyI C'CwwG_G
Cuts at: 513 1065 2004 2030 2172 2788 3048 3308 513
Size: 552 939 26 142 616 260 260 3074
Fragments arranged by size:
3074 939 616 552 260 260 142 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2889 3042 3896 3913 4066 4251 4696
Size: 1255 1309 153 854 17 153 185 445
Cuts at: 4696 325
Size: 1498
Fragments arranged by size:
1498 1309 1255 854 445 185 153 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 4115 5827
Size: 274 80 33 51 1443 519 1619 1712
Cuts at: 5827 96
Size: 138
Fragments arranged by size:
1712 1619 1443 519 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3017 3049 5829 5861 3017
Size: 32 2780 32 3025
Fragments arranged by size:
3025 2780 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2266 1316
Size: 950 4919
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2684 3046 2684
Size: 362 5507
XhoI C'TCGA_G
Cuts at: 1943 2045 1943
Size: 102 5767
XmaI C'CCGG_G
Cuts at: 1870 2110 2395 2421 3194 1870
Size: 240 285 26 773 4545
Fragments arranged by size:
4545 773 285 240 26
XmnI GAAnn'nnTTC
Cuts at: 2156 3998 2156
Size: 1842 4027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI