Restriction map for
pFN21AB8986
(Circular) (Six-base) MAPSORT of: pFN21AB8986.seq Check: 1283 from: 1 to: 5866
pFN21AB8986 5866 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 2481 3187 2481
Size: 706 5160
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2719 3929 5313 5581 1632
Size: 1087 1210 1384 268 1917
Fragments arranged by size:
1917 1384 1210 1087 268
AflII C'TTAA_G
Cuts at: 820 1017 2867 820
Size: 197 1850 3819
Fragments arranged by size:
3819 1850 197
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 3138 1166
Size: 1972 3894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 2752 3609 5181 2752
Size: 857 1572 3437
Fragments arranged by size:
3437 1572 857
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 2245 2408 2686 3175 3401 3553 5662 1493
Size: 752 163 278 489 226 152 2109 1697
Fragments arranged by size:
2109 1697 752 489 278 226 163 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 2639 3191 1870
Size: 73 696 552 4545
Fragments arranged by size:
4545 696 552 73
AvrII C'CTAG_G
Cuts at: 2551 2551
Size: 5866
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2481 3187 4642 618
Size: 325 217 627 694 706 1455 1842
Fragments arranged by size:
1842 1455 706 694 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2060 3185 1391
Size: 669 1125 4072
Fragments arranged by size:
4072 1125 669
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4895
BbvCI CC'TCA_GC
Cuts at: 2154 2154
Size: 5866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BfrBI ATG'CAT
Cuts at: 2981 3152 2981
Size: 171 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 4477 136
Size: 107 122 71 631 605 418 2387 1525
Fragments arranged by size:
2387 1525 631 605 418 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 3927 5083 1790
Size: 2137 1156 2573
Fragments arranged by size:
2573 2137 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2584 2883 3597
Size: 416 53 83 399 24 1167 299 714
Cuts at: 3597 4555 442
Size: 958 1753
Fragments arranged by size:
1753 1167 958 714 416 399 299 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2633 2879 4526 1239
Size: 800 594 246 1647 2579
Fragments arranged by size:
2579 1647 800 594 246
Bpu10I CC'TnA_GC
Cuts at: 2154 2154
Size: 5866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4529 882
Size: 617 3030 2219
Fragments arranged by size:
3030 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2538 2556 3673 4055
Size: 53 83 186 936 1005 18 1117 382
Cuts at: 4055 4690 275
Size: 635 1451
Fragments arranged by size:
1451 1117 1005 936 635 382 186 83
53 18
BsaWI w'CCGG_w
Cuts at: 2520 4299 4697 4971 5118 5529 2520
Size: 1779 398 274 147 411 2857
Fragments arranged by size:
2857 1779 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5641
Fragments arranged by size:
5641 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3064 1186
Size: 1878 3988
BseYI C'CCAG_C
Cuts at: 2987 5069 5565 2987
Size: 2082 496 3288
Fragments arranged by size:
3288 2082 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2549 1163
Size: 573 813 4480
Fragments arranged by size:
4480 813 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2060 2670 3927 4012 5083 2060
Size: 610 1257 85 1071 2843
Fragments arranged by size:
2843 1257 1071 610 85
BsmI GAATG_Cn'
Cuts at: 2627 3377 3470 2627
Size: 750 93 5023
Fragments arranged by size:
5023 750 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2855 2855
Size: 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2670 3185 3927 4012 5083 1391
Size: 399 270 610 515 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 610 515 399 270 85
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3221 844
Size: 2377 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3753 1394
Size: 429 1930 3507
Fragments arranged by size:
3507 1930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2680 3152 4361 4535 65
Size: 1183 1432 472 1209 174 1396
Fragments arranged by size:
1432 1396 1209 1183 472 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4510 4697 1700
Size: 110 2700 187 2869
Fragments arranged by size:
2869 2700 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 3136 3926 4938 3136
Size: 790 1012 4064
Fragments arranged by size:
4064 1012 790
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5866
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstXI CCAn_nnnn'nTGG
Cuts at: 2236 2497 2578 3023 2236
Size: 261 81 445 5079
Fragments arranged by size:
5079 445 261 81
BstYI r'GATC_y
Cuts at: 1972 2908 3196 3563 3948 3965 5417 5503 5515
Size: 936 288 367 385 17 1452 86 12
Cuts at: 5515 5861 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 936 385 367 346 288 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 4279
Fragments arranged by size:
4279 1035 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2180 4873 809
Size: 507 864 2693 1802
Fragments arranged by size:
2693 1802 864 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2487 2499
Size: 54 1425 50 275 18 252 405 12
Cuts at: 2499 3313 4202 5831 8
Size: 814 889 1629 43
Fragments arranged by size:
1629 1425 889 814 405 275 252 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2831 3796 1353
Size: 695 783 965 3423
Fragments arranged by size:
3423 965 783 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2058 2058
Size: 5866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2633 2719 2879 3929 4526 5313
Size: 393 407 594 86 160 1050 597 787
Cuts at: 5313 5581 1239
Size: 268 1524
Fragments arranged by size:
1524 1050 787 597 594 407 393 268
160 86
EcoO109I rG'GnC_Cy
Cuts at: 2091 2386 2644 2091
Size: 295 258 5313
Fragments arranged by size:
5313 295 258
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 3055 5013 1678
Size: 1377 1958 2531
Fragments arranged by size:
2531 1958 1377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2893 2925 3059 3091 4507
Size: 32 135 32 1321 32 134 32 1416
Cuts at: 4507 4539 4581 4613 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1416 1321 135 134 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3210 3456 669
Size: 988 281 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 2485 3191 2485
Size: 706 5160
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4980 5164 1305
Size: 3675 184 2007
Fragments arranged by size:
3675 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 2489 10
Size: 54 1425 343 252 405 3387
Fragments arranged by size:
3387 1425 405 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 3021 3105 3138 3824 4183 4342
Size: 648 1410 445 84 33 686 359 159
Cuts at: 4342 518
Size: 2042
Fragments arranged by size:
2042 1410 686 648 445 359 159 84
33
MspA1I CmG'CkG
Cuts at: 1606 3282 3959 5107 5352 1606
Size: 1676 677 1148 245 2120
Fragments arranged by size:
2120 1676 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 4279
Fragments arranged by size:
4279 1035 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NsiI A_TGCA'T
Cuts at: 2983 3154 2983
Size: 171 5695
NspI r_CATG'y
Cuts at: 1132 1229 3224 4769 1132
Size: 97 1995 1545 2229
Fragments arranged by size:
2229 1995 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5866
PfoI T'CCnGG_A
Cuts at: 2649 2856 2649
Size: 207 5659
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2644 2644
Size: 5866
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PstI C_TGCA'G
Cuts at: 830 3218 830
Size: 2388 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
RsrII CG'GwC_CG
Cuts at: 2523 2523
Size: 5866
SacI G_AGCT'C
Cuts at: 2060 2060
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SapI GCTCTTCn'nnn_
Cuts at: 2048 2048
Size: 5866
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 3214 4349 5030 5221 826
Size: 219 2169 1135 681 191 1471
Fragments arranged by size:
2169 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2090 2090
Size: 5866
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2867 3970 4871 5133 820
Size: 197 926 924 1103 901 262 1553
Fragments arranged by size:
1553 1103 926 924 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3224 1132
Size: 2092 3774
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 2159 1517
Size: 642 5224
StyI C'CwwG_G
Cuts at: 513 1065 2100 2551 2626 3305 513
Size: 552 1035 451 75 679 3074
Fragments arranged by size:
3074 1035 679 552 451 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2061 3893 3910 4063 4248 4693 325
Size: 1255 481 1832 17 153 185 445 1498
Fragments arranged by size:
1832 1498 1255 481 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4112 5824 96
Size: 274 80 33 51 1443 2135 1712 138
Fragments arranged by size:
2135 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 3140 2236
Size: 904 4962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BplI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI PvuII SacII SanDI SexAI SfoI
SrfI SwaI