Restriction map for
pFN21AB8984
(Circular) (Six-base) MAPSORT of: pFN21AB8984.seq Check: 8947 from: 1 to: 5863
pFN21AB8984 5863 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3061 3061
Size: 5863
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3206 1937
Size: 1269 4594
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3926 5310 5578 1632
Size: 2294 1384 268 1917
Fragments arranged by size:
2294 1917 1384 268
AfeI AGC'GCT
Cuts at: 2354 2354
Size: 5863
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 2033 1166
Size: 867 4996
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5863
AlwNI CAG_nnn'CTG
Cuts at: 2634 3606 5178 2634
Size: 972 1572 3319
Fragments arranged by size:
3319 1572 972
ApaI G_GGCC'C
Cuts at: 2403 2536 3010 2403
Size: 133 474 5256
Fragments arranged by size:
5256 474 133
ApaLI G'TGCA_C
Cuts at: 3920 5076 3920
Size: 1156 4707
ApoI r'AATT_y
Cuts at: 1493 3172 3398 3550 5659 1493
Size: 1679 226 152 2109 1697
Fragments arranged by size:
2109 1697 1679 226 152
AscI GG'CGCG_CC
Cuts at: 2135 2135
Size: 5863
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 2939 3188 1870
Size: 73 316 680 249 4545
Fragments arranged by size:
4545 680 316 249 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2546 3184 4639 618
Size: 325 217 627 405 354 638 1455 1842
Fragments arranged by size:
1842 1455 638 627 405 354 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2210 2403 2536 2672 3010 3081 3182
Size: 654 165 193 133 136 338 71 101
Cuts at: 3182 1391
Size: 4072
Fragments arranged by size:
4072 654 338 193 165 136 133 101
71
BbeI G_GCGC'C
Cuts at: 2550 2550
Size: 5863
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2869 2893 928
Size: 971 970 24 3898
Fragments arranged by size:
3898 971 970 24
BbvCI CC'TCA_GC
Cuts at: 2627 2627
Size: 5863
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4054 4088 1903
Size: 34 2117 34 3678
Fragments arranged by size:
3678 2117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3755 4965 3755
Size: 1210 4653
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2417 2667 4474
Size: 107 122 71 631 605 745 250 1807
Cuts at: 4474 136
Size: 1525
Fragments arranged by size:
1807 1525 745 631 605 250 122 107
71
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 2366 3280 2366
Size: 914 4949
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2403 2536 3010 3924 5080 1790
Size: 405 208 133 474 914 1156 2573
Fragments arranged by size:
2573 1156 914 474 405 208 133
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3594 4552 442
Size: 416 53 83 399 24 2177 958 1753
Fragments arranged by size:
2177 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4523 1239
Size: 3284 2579
Bpu10I CC'TnA_GC
Cuts at: 2117 2204 2627 2117
Size: 87 423 5353
Fragments arranged by size:
5353 423 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2897 3988 4853 5151 2897
Size: 1091 865 298 3609
Fragments arranged by size:
3609 1091 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4526 882
Size: 617 3027 2219
Fragments arranged by size:
3027 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2035 3654 493
Size: 711 831 1619 2702
Fragments arranged by size:
2702 1619 831 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2318 2547 3670 4052
Size: 53 83 186 936 785 229 1123 382
Cuts at: 4052 4687 275
Size: 635 1451
Fragments arranged by size:
1451 1123 936 785 635 382 229 186
83 53
BsaWI w'CCGG_w
Cuts at: 2165 2693 4296 4694 4968 5115 5526 2165
Size: 528 1603 398 274 147 411 2502
Fragments arranged by size:
2502 1603 528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5638
Fragments arranged by size:
5638 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3007 1186
Size: 1821 4042
BseYI C'CCAG_C
Cuts at: 2188 2248 2470 2529 2535 2976 5066 5562 2188
Size: 60 222 59 6 441 2090 496 2489
Fragments arranged by size:
2489 2090 496 441 222 60 59 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2038 1163
Size: 573 302 4988
Fragments arranged by size:
4988 573 302
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2132 2214 2687 3313 4074 5102
Size: 275 186 131 82 473 626 761 1028
Cuts at: 5102 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 626 473 275 186 131
82
BsiHKAI G_wGCw'C
Cuts at: 2759 3924 4009 5080 2759
Size: 1165 85 1071 3542
Fragments arranged by size:
3542 1165 1071 85
BsmI GAATG_Cn'
Cuts at: 3374 3467 3374
Size: 93 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2195 2210 2403 2536 2672 2759
Size: 399 255 150 15 193 133 136 87
Cuts at: 2759 3010 3081 3182 3924 4009 5080 1391
Size: 251 71 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 255 251 193 150
136 133 101 87 85 71 15
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 2733 3752 5482 2733
Size: 1019 1730 3114
Fragments arranged by size:
3114 1730 1019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 3061 3218 844
Size: 1349 868 157 3489
Fragments arranged by size:
3489 1349 868 157
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4358 4532 65
Size: 1183 3110 174 1396
Fragments arranged by size:
3110 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 2081 2131 2570 4507 4694 1700
Size: 110 243 28 50 439 1937 187 2869
Fragments arranged by size:
2869 1937 439 243 187 110 50 28
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 2135 2231 2457 1925
Size: 210 96 226 5331
Fragments arranged by size:
5331 226 210 96
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2511 794
Size: 1717 4146
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 2161 2620 1184
Size: 977 459 4427
Fragments arranged by size:
4427 977 459
BstXI CCAn_nnnn'nTGG
Cuts at: 2347 2347
Size: 5863
BstYI r'GATC_y
Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858
Size: 1221 367 385 17 1452 86 12 346
Cuts at: 5858 1972
Size: 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2340 2346 2681 2950 3114 513
Size: 552 939 336 6 335 269 164 3262
Fragments arranged by size:
3262 939 552 336 335 269 164 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2295 487
Size: 683 56 1069 4055
Fragments arranged by size:
4055 1069 683 56
BtsI GCAGTG_nn'
Cuts at: 2818 3379 4173 4193 2818
Size: 561 794 20 4488
Fragments arranged by size:
4488 794 561 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2873 4870 809
Size: 507 1557 1997 1802
Fragments arranged by size:
1997 1802 1557 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2079 2175
Size: 54 1425 50 275 18 225 24 96
Cuts at: 2175 2211 2343 2684 2748 3310 4199 5828 8
Size: 36 132 341 64 562 889 1629 43
Fragments arranged by size:
1629 1425 889 562 341 275 225 132
96 64 54 50 43 36 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2211 2684 3310 1537
Size: 275 399 473 626 4090
Fragments arranged by size:
4090 626 473 399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 3793 1353
Size: 1103 1337 3423
Fragments arranged by size:
3423 1337 1103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4450 4824 4970 387
Size: 1064 138 435 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3926 4523 5310 5578 1239
Size: 393 2294 597 787 268 1524
Fragments arranged by size:
2294 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2989 2989
Size: 5863
EcoO109I rG'GnC_Cy
Cuts at: 2400 2824 2998 3006 3007 2400
Size: 424 174 8 1 5256
Fragments arranged by size:
5256 424 174 8 1
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 5863
FseI GG_CCGG'CC
Cuts at: 2057 2057
Size: 5863
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 2356 2366 2423 2550 2662 3114 5010 1678
Size: 678 10 57 127 112 452 1896 2531
Fragments arranged by size:
2531 1896 678 452 127 112 57 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2901 2933 4504 4536 4578
Size: 32 135 32 1329 32 1571 32 42
Cuts at: 4578 4610 1373
Size: 32 2626
Fragments arranged by size:
2626 1571 1329 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KasI G'GCGC_C
Cuts at: 2546 2546
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4977 5161 1305
Size: 3672 184 2007
Fragments arranged by size:
3672 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4041
Fragments arranged by size:
4041 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2069 2345 2738 2750 3821 4180
Size: 648 867 36 276 393 12 1071 359
Cuts at: 4180 4339 518
Size: 159 2042
Fragments arranged by size:
2042 1071 867 648 393 359 276 159
36 12
MspA1I CmG'CkG
Cuts at: 1606 2392 2406 2508 2778 3105 3279 3956 5104
Size: 786 14 102 270 327 174 677 1148
Cuts at: 5104 5349 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 786 677 327 270 245 174
102 14
NaeI GCC'GGC
Cuts at: 2055 2572 2055
Size: 517 5346
NarI GG'CG_CC
Cuts at: 2547 2547
Size: 5863
NcoI C'CATG_G
Cuts at: 513 1065 2004 2340 513
Size: 552 939 336 4036
Fragments arranged by size:
4036 939 552 336
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NgoMIV G'CCGG_C
Cuts at: 2053 2570 2053
Size: 517 5346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 2527 2644 3221 4766 1132
Size: 97 1298 117 577 1545 2229
Fragments arranged by size:
2229 1545 1298 577 117 97
PasI CC'CwG_GG
Cuts at: 1348 2107 2871 1348
Size: 759 764 4340
Fragments arranged by size:
4340 764 759
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5863
PfoI T'CCnGG_A
Cuts at: 2862 2987 2862
Size: 125 5738
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2824 2998 2824
Size: 174 5689
PshAI GACnn'nnGTC
Cuts at: 1613 2741 1613
Size: 1128 4735
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PspOMI G'GGCC_C
Cuts at: 2399 2532 3006 2399
Size: 133 474 5256
Fragments arranged by size:
5256 474 133
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2015 2047 2015
Size: 32 5831
PstI C_TGCA'G
Cuts at: 830 3215 830
Size: 2385 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 2392 2508 2392
Size: 116 5747
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SanDI GG'GwC_CC
Cuts at: 2998 2998
Size: 5863
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 2495 4111 5823 2495
Size: 1616 1712 2535
Fragments arranged by size:
2535 1712 1616
SfcI C'TryA_G
Cuts at: 826 1045 3211 4346 5027 5218 826
Size: 219 2166 1135 681 191 1471
Fragments arranged by size:
2166 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2548 2548
Size: 5863
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 3190 1872
Size: 1318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2912 3967 4868 5130 820
Size: 197 926 969 1055 901 262 1553
Fragments arranged by size:
1553 1055 969 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 2527 3221 1132
Size: 1395 694 3774
Fragments arranged by size:
3774 1395 694
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5863
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 3002 3302 513
Size: 552 939 336 662 300 3074
Fragments arranged by size:
3074 939 662 552 336 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2608 2801 3890 3907 4060 4245 4690
Size: 1255 1028 193 1089 17 153 185 445
Cuts at: 4690 325
Size: 1498
Fragments arranged by size:
1498 1255 1089 1028 445 193 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2493 4109 5821
Size: 274 80 33 51 1443 516 1616 1712
Cuts at: 5821 96
Size: 138
Fragments arranged by size:
1712 1616 1443 516 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5823 5855 5823
Size: 32 5831
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2185
Size: 5863
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 3188 1870
Size: 1318 4545
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI
PmlI PspXI RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI