Restriction map for
pFN21AB8981
(Circular) (Six-base) MAPSORT of: pFN21AB8981.seq Check: 7456 from: 1 to: 5836
pFN21AB8981 5836 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2964 3033 3899 5283 5551 1632
Size: 1332 69 866 1384 268 1917
Fragments arranged by size:
1917 1384 1332 866 268 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 3056 3579 5151 3056
Size: 523 1572 3741
Fragments arranged by size:
3741 1572 523
ApaLI G'TGCA_C
Cuts at: 2037 3893 5049 2037
Size: 1856 1156 2824
Fragments arranged by size:
2824 1856 1156
ApoI r'AATT_y
Cuts at: 1493 2252 2334 2476 3069 3145 3371 3523 5632
Size: 759 82 142 593 76 226 152 2109
Cuts at: 5632 1493
Size: 1697
Fragments arranged by size:
2109 1697 759 593 226 152 142 82
76
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 3161 1870
Size: 73 1218 4545
Fragments arranged by size:
4545 1218 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3157 4612 618
Size: 325 217 627 1370 1455 1842
Fragments arranged by size:
1842 1455 1370 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3155 1391
Size: 1764 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2883 928
Size: 971 984 3881
Fragments arranged by size:
3881 984 971
BbvCI CC'TCA_GC
Cuts at: 2551 2620 2689 2758 2827 2551
Size: 69 69 69 69 5560
Fragments arranged by size:
5560 69 69 69 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4447 136
Size: 107 122 71 631 605 2775 1525
Fragments arranged by size:
2775 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 2041 3897 5053 1790
Size: 251 1856 1156 2573
Fragments arranged by size:
2573 1856 1156 251
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2162 1056
Size: 1106 4730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4496 1239
Size: 3257 2579
Bpu10I CC'TnA_GC
Cuts at: 2220 2551 2620 2689 2758 2827 2220
Size: 331 69 69 69 69 5229
Fragments arranged by size:
5229 331 69 69 69 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3961 4826 5124 3961
Size: 865 298 4673
Fragments arranged by size:
4673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2558 2627 2696 2765 2834 2903 4499
Size: 617 1059 69 69 69 69 69 1596
Cuts at: 4499 882
Size: 2219
Fragments arranged by size:
2219 1596 1059 617 69 69 69 69
69
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2521 2551 2590 2620 2659
Size: 30 165 30 1118 30 39 30 39
Cuts at: 2659 2689 2728 2758 2866 2896 3037 3067 1178
Size: 30 39 30 108 30 141 30 3947
Fragments arranged by size:
3947 1118 165 141 108 39 39 39
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5836
BseYI C'CCAG_C
Cuts at: 2482 2896 5039 5535 2482
Size: 414 2143 496 2783
Fragments arranged by size:
2783 2143 496 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2970 1163
Size: 573 450 784 4029
Fragments arranged by size:
4029 784 573 450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2041 3897 3982 5053 2041
Size: 1856 85 1071 2824
Fragments arranged by size:
2824 1856 1071 85
BsmI GAATG_Cn'
Cuts at: 2413 3347 3440 2413
Size: 934 93 4809
Fragments arranged by size:
4809 934 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 3155 3897 3982 5053 1391
Size: 399 251 1114 742 85 1071 2174
Fragments arranged by size:
2174 1114 1071 742 399 251 85
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 2083 3725 5455 2083
Size: 1642 1730 2464
Fragments arranged by size:
2464 1730 1642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4331 4505 65
Size: 1183 3083 174 1396
Fragments arranged by size:
3083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4480 4667 1700
Size: 110 2670 187 2869
Fragments arranged by size:
2869 2670 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2045 794
Size: 1251 4585
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831
Size: 1194 367 385 17 1452 86 12 346
Cuts at: 5831 1972
Size: 1977
Fragments arranged by size:
1977 1452 1194 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2305 3352 4146 4166 2305
Size: 1047 794 20 3975
Fragments arranged by size:
3975 1047 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DraIII CAC_nnn'GTG
Cuts at: 2045 2045
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3058 3283 4172
Size: 54 1425 50 275 18 1228 225 889
Cuts at: 4172 5801 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1228 889 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2455 3766 1353
Size: 1102 1311 3423
Fragments arranged by size:
3423 1311 1102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2812 4423 4797 4943 387
Size: 1064 138 1223 1611 374 146 1280
Fragments arranged by size:
1611 1280 1223 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2964 3033 3899 4496 5283 5551 1239
Size: 393 1332 69 866 597 787 268 1524
Fragments arranged by size:
1524 1332 866 787 597 393 268 69
EcoRI G'AATT_C
Cuts at: 2476 3145 3523 2476
Size: 669 378 4789
Fragments arranged by size:
4789 669 378
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 4983 1678
Size: 3305 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2804 2836 4477 4509 4551
Size: 32 135 32 1232 32 1641 32 42
Cuts at: 4551 4583 1373
Size: 32 2626
Fragments arranged by size:
2626 1641 1232 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 2440 3180 3426 669
Size: 988 281 370 132 740 246 3079
Fragments arranged by size:
3079 988 740 370 281 246 132
HindIII A'AGCT_T
Cuts at: 748 2222 748
Size: 1474 4362
HpaI GTT'AAC
Cuts at: 2440 3426 2440
Size: 986 4850
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3060 10
Size: 54 1425 343 1228 2786
Fragments arranged by size:
2786 1425 1228 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3794 4153 4312 518
Size: 648 2628 359 159 2042
Fragments arranged by size:
2628 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2504 2573 2642 2711 2780 2849 2918
Size: 414 484 69 69 69 69 69 69
Cuts at: 2918 2984 3252 3929 5077 5322 1606
Size: 66 268 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 484 414 268 245 69
69 69 69 69 69 66
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 2158 1052
Size: 1106 4730
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5836
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PfoI T'CCnGG_A
Cuts at: 2149 2149
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 3188 830
Size: 2358 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2504 2573 2642 2711 2780 2849 2918 2984 2504
Size: 69 69 69 69 69 69 66 5356
Fragments arranged by size:
5356 69 69 69 69 69 69 66
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 2455 2455
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2212 2454 3184 4319 5000 5191 826
Size: 219 1167 242 730 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 730 681 242 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3940 4841 5103 820
Size: 197 926 1997 901 262 1553
Fragments arranged by size:
1997 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 2218 1517
Size: 701 5135
StyI C'CwwG_G
Cuts at: 513 1065 3275 513
Size: 552 2210 3074
Fragments arranged by size:
3074 2210 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2134 2527 2596 2665 2734 2803 2872
Size: 1255 554 393 69 69 69 69 69
Cuts at: 2872 3001 3004 3863 3880 4033 4218 4663 325
Size: 129 3 859 17 153 185 445 1498
Fragments arranged by size:
1498 1255 859 554 445 393 185 153
129 69 69 69 69 69 17 3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5796 5828 5796
Size: 32 5804
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 2174 3965 2174
Size: 1791 4045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI