Restriction map for
pFN21AB8980
(Circular) (Six-base) MAPSORT of: pFN21AB8980.seq Check: 746 from: 1 to: 5833
pFN21AB8980 5833 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AfeI AGC'GCT
Cuts at: 2538 2538
Size: 5833
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2273 1184
Size: 1089 4744
AlwNI CAG_nnn'CTG
Cuts at: 3124 3576 5148 3124
Size: 452 1572 3809
Fragments arranged by size:
3809 1572 452
ApaI G_GGCC'C
Cuts at: 2731 2731
Size: 5833
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2015 2486 3142 3368 3520 5629 1493
Size: 522 471 656 226 152 2109 1697
Fragments arranged by size:
2109 1697 656 522 471 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 3158 1870
Size: 73 339 876 4545
Fragments arranged by size:
4545 876 339 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2257 2267 2436 3154 4609
Size: 325 217 627 470 10 169 718 1455
Cuts at: 4609 618
Size: 1842
Fragments arranged by size:
1842 1455 718 627 470 325 217 169
10
BanII G_rGCy'C
Cuts at: 1391 2234 2731 3152 1391
Size: 843 497 421 4072
Fragments arranged by size:
4072 843 497 421
BbeI G_GCGC'C
Cuts at: 2271 2440 2271
Size: 169 5664
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2699 928
Size: 971 800 4062
Fragments arranged by size:
4062 971 800
BbvCI CC'TCA_GC
Cuts at: 2384 2384
Size: 5833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 4444 136
Size: 107 122 71 631 605 566 2206 1525
Fragments arranged by size:
2206 1525 631 605 566 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2731 3894 5050 1790
Size: 472 469 1163 1156 2573
Fragments arranged by size:
2573 1163 1156 472 469
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2605 2816 3024
Size: 416 53 83 399 24 1188 211 208
Cuts at: 3024 3564 4522 442
Size: 540 958 1753
Fragments arranged by size:
1753 1188 958 540 416 399 211 208
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2756 4493 1239
Size: 1517 1737 2579
Fragments arranged by size:
2579 1737 1517
Bpu10I CC'TnA_GC
Cuts at: 2384 2714 2384
Size: 330 5503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2975 3958 4823 5121 2675
Size: 300 983 865 298 3387
Fragments arranged by size:
3387 983 865 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4496 882
Size: 617 2997 2219
Fragments arranged by size:
2997 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 2437 3640 4022
Size: 53 83 186 936 735 169 1203 382
Cuts at: 4022 4657 275
Size: 635 1451
Fragments arranged by size:
1451 1203 936 735 635 382 186 169
83 53
BsaWI w'CCGG_w
Cuts at: 2583 4266 4664 4938 5085 5496 2583
Size: 1683 398 274 147 411 2920
Fragments arranged by size:
2920 1683 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 2690 1186
Size: 956 548 4329
Fragments arranged by size:
4329 956 548
BseYI C'CCAG_C
Cuts at: 2174 5036 5532 2174
Size: 2862 496 2475
Fragments arranged by size:
2862 2475 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2218 2340 3283 4044 5072 1540
Size: 275 186 217 122 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 217 186 122
BsiHKAI G_wGCw'C
Cuts at: 3894 3979 5050 3894
Size: 85 1071 4677
Fragments arranged by size:
4677 1071 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
BsmBI CGTCTCn'nnnn_
Cuts at: 2725 2725
Size: 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2262 2731 3152 3894 3979 5050
Size: 399 444 28 469 421 742 85 1071
Cuts at: 5050 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 469 444 421 399 85
28
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 844
Size: 2344 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2848 4328 4502 65
Size: 1183 1600 1480 174 1396
Fragments arranged by size:
1600 1480 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2264 2335 2369 4477 4664 1700
Size: 110 454 71 34 2108 187 2869
Fragments arranged by size:
2869 2108 454 187 110 71 34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstXI CCAn_nnnn'nTGG
Cuts at: 2210 2918 2210
Size: 708 5125
BstYI r'GATC_y
Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828
Size: 1191 367 385 17 1452 86 12 346
Cuts at: 5828 1972
Size: 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2946 2946
Size: 5833
BtgI C'CryG_G
Cuts at: 513 1065 2203 2235 2245 513
Size: 552 1138 32 10 4101
Fragments arranged by size:
4101 1138 552 32 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2313 2337
Size: 54 1425 50 275 18 385 98 24
Cuts at: 2337 3280 4169 5798 8
Size: 943 889 1629 43
Fragments arranged by size:
1629 1425 943 889 385 275 98 54
50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2215 2337 3280 1537
Size: 275 403 122 943 4090
Fragments arranged by size:
4090 943 403 275 122
EarI CTCTTCn'nnn_
Cuts at: 1353 2682 3763 1353
Size: 1329 1081 3423
Fragments arranged by size:
3423 1329 1081
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2756 3896 4493 5280 5548 1239
Size: 393 1124 1140 597 787 268 1524
Fragments arranged by size:
1524 1140 1124 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2727 2728 2950 2959 2727
Size: 1 222 9 5601
Fragments arranged by size:
5601 222 9 1
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 5833
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2211 2271 2440 2540 4980 1678
Size: 533 60 169 100 2440 2531
Fragments arranged by size:
2531 2440 533 169 100 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2137 2169 4474 4506 4548
Size: 32 135 32 565 32 2305 32 42
Cuts at: 4548 4580 1373
Size: 32 2626
Fragments arranged by size:
2626 2305 565 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3177 3423 669
Size: 988 281 963 276 246 3079
Fragments arranged by size:
3079 988 963 281 276 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 2267 2436 2267
Size: 169 5664
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4947 5131 1305
Size: 3642 184 2007
Fragments arranged by size:
3642 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3791 4150 4309 518
Size: 648 2625 359 159 2042
Fragments arranged by size:
2625 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2237 2247 2421 3071 3249 3926 5074 5319
Size: 631 10 174 650 178 677 1148 245
Cuts at: 5319 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 650 631 245 178 174
10
NaeI GCC'GGC
Cuts at: 2266 2337 2266
Size: 71 5762
NarI GG'CG_CC
Cuts at: 2268 2437 2268
Size: 169 5664
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5281
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NgoMIV G'CCGG_C
Cuts at: 2264 2335 2264
Size: 71 5762
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 2215 3280 2215
Size: 1065 4768
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2959 2959
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PspOMI G'GGCC_C
Cuts at: 2727 2727
Size: 5833
PstI C_TGCA'G
Cuts at: 830 2277 3009 3185 830
Size: 1447 732 176 3478
Fragments arranged by size:
3478 1447 732 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 3071 3071
Size: 5833
SacII CC_GC'GG
Cuts at: 2238 2248 2238
Size: 10 5823
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2273 3005 3181 4316 4997 5188 826
Size: 219 1228 732 176 1135 681 191 1471
Fragments arranged by size:
1471 1228 1135 732 681 219 191 176
SfoI GGC'GCC
Cuts at: 2269 2438 2269
Size: 169 5664
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 2264 1700
Size: 564 5269
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 2954 3937 4838 5100 820
Size: 197 926 711 300 983 901 262 1553
Fragments arranged by size:
1553 983 926 901 711 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 3272 513
Size: 552 2207 3074
Fragments arranged by size:
3074 2207 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2893 3043 3860 3877 4030 4215 4660
Size: 1255 1313 150 817 17 153 185 445
Cuts at: 4660 325
Size: 1498
Fragments arranged by size:
1498 1313 1255 817 445 185 153 150
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4079 5791 96
Size: 274 80 33 51 1443 2102 1712 138
Fragments arranged by size:
2102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2381 2413 5793 5825 2381
Size: 32 3380 32 2389
Fragments arranged by size:
3380 2389 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 2470 3169 2470
Size: 699 5134
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI XcmI