Restriction map for
pFN21AB8978
(Circular) (Six-base) MAPSORT of: pFN21AB8978.seq Check: 5424 from: 1 to: 5827
pFN21AB8978 5827 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2857 2857
Size: 5827
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 3092 3117 3890 5274 5542 1632
Size: 611 849 25 773 1384 268 1917
Fragments arranged by size:
1917 1384 849 773 611 268 25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 2867 1166
Size: 1701 4126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 3570 5142 3570
Size: 1572 4255
ApaI G_GGCC'C
Cuts at: 2131 2131
Size: 5827
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2105 2813 3136 3362 3514 5623 1493
Size: 612 708 323 226 152 2109 1697
Fragments arranged by size:
2109 1697 708 612 323 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2671 3152 1870
Size: 73 728 481 4545
Fragments arranged by size:
4545 728 481 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3148 4603 618
Size: 325 217 627 1361 1455 1842
Fragments arranged by size:
1842 1455 1361 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2131 2302 3094 3146 1391
Size: 740 171 792 52 4072
Fragments arranged by size:
4072 792 740 171 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3098 928
Size: 971 1199 3657
Fragments arranged by size:
3657 1199 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4018 4052 1903
Size: 34 50 34 1997 34 3678
Fragments arranged by size:
3678 1997 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 2490 1548
Size: 942 4885
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2131 3888 5044 1790
Size: 341 1757 1156 2573
Fragments arranged by size:
2573 1757 1156 341
BmgBI CAC'GTC
Cuts at: 1318 2113 1318
Size: 795 5032
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3558 4516 442
Size: 416 53 83 399 24 2141 958 1753
Fragments arranged by size:
2141 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4487 1239
Size: 3248 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3010 4490 882
Size: 617 1511 1480 2219
Fragments arranged by size:
2219 1511 1480 617
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3634 4016 4651 275
Size: 53 83 186 936 2101 382 635 1451
Fragments arranged by size:
2101 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4260 4658 4932 5079 5490 4260
Size: 398 274 147 411 4597
Fragments arranged by size:
4597 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3036 3066 1178
Size: 30 165 30 1633 30 3939
Fragments arranged by size:
3939 1633 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3044 1186
Size: 1858 3969
BseYI C'CCAG_C
Cuts at: 5030 5526 5030
Size: 496 5331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2095 3888 3973 5044 2095
Size: 1793 85 1071 2878
Fragments arranged by size:
2878 1793 1071 85
BsmI GAATG_Cn'
Cuts at: 2338 3338 3431 2338
Size: 1000 93 4734
Fragments arranged by size:
4734 1000 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2131 2302 3094 3146 3888 3973
Size: 399 305 36 171 792 52 742 85
Cuts at: 3973 5044 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 792 742 399 305 171 85
52 36
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 2748 3716 5446 2748
Size: 968 1730 3129
Fragments arranged by size:
3129 1730 968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2253 2857 3182 844
Size: 1409 604 325 3489
Fragments arranged by size:
3489 1409 604 325
BsrBI CCG'CTC
Cuts at: 1394 1823 2052 2146 3714 1394
Size: 429 229 94 1568 3507
Fragments arranged by size:
3507 1568 429 229 94
BsrDI GCAATG_nn'
Cuts at: 65 1248 2343 4322 4496 65
Size: 1183 1095 1979 174 1396
Fragments arranged by size:
1979 1396 1183 1095 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4471 4658 1700
Size: 110 2661 187 2869
Fragments arranged by size:
2869 2661 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 2589 3887 4899 2589
Size: 1298 1012 3517
Fragments arranged by size:
3517 1298 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 2464 1184
Size: 1280 4547
BstXI CCAn_nnnn'nTGG
Cuts at: 2595 2851 2595
Size: 256 5571
BstYI r'GATC_y
Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822
Size: 1185 367 385 17 1452 86 12 346
Cuts at: 5822 1972
Size: 1977
Fragments arranged by size:
1977 1452 1185 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2733 2893 3343 4137 4157 2733
Size: 160 450 794 20 4403
Fragments arranged by size:
4403 794 450 160 20
ClaI AT'CG_AT
Cuts at: 1554 2412 1554
Size: 858 4969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2056 2091 529
Size: 35 1492 35 4265
Fragments arranged by size:
4265 1492 35 35
DraI TTT'AAA
Cuts at: 2451 3131 2451
Size: 680 5147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 3274 4163
Size: 54 1425 50 275 18 426 1018 889
Cuts at: 4163 5792 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1018 889 426 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2708 2771 3757 1353
Size: 1355 63 986 3423
Fragments arranged by size:
3423 1355 986 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2397 4414 4788 4934 387
Size: 1064 138 808 2017 374 146 1280
Fragments arranged by size:
2017 1280 1064 808 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 3092 3117 3890 4487 5274 5542
Size: 393 611 849 25 773 597 787 268
Cuts at: 5542 1239
Size: 1524
Fragments arranged by size:
1524 849 787 773 611 597 393 268
25
EcoO109I rG'GnC_Cy
Cuts at: 2127 2127
Size: 5827
EcoRI G'AATT_C
Cuts at: 2813 3136 3514 2813
Size: 323 378 5126
Fragments arranged by size:
5126 378 323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3122 3122
Size: 5827
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 4974 1678
Size: 3296 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2863 2895 4468 4500 4542
Size: 32 135 32 1291 32 1573 32 42
Cuts at: 4542 4574 1373
Size: 32 2626
Fragments arranged by size:
2626 1573 1291 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2849 3171 3417 669
Size: 988 281 911 322 246 3079
Fragments arranged by size:
3079 988 911 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3081 3426 3081
Size: 345 5482
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2258 10
Size: 54 1425 343 426 3579
Fragments arranged by size:
3579 1425 426 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2593 2615 2747 2867 3785 4144 4303
Size: 648 1427 22 132 120 918 359 159
Cuts at: 4303 518
Size: 2042
Fragments arranged by size:
2042 1427 918 648 359 159 132 120
22
MspA1I CmG'CkG
Cuts at: 1606 2647 3243 3920 5068 5313 1606
Size: 1041 596 677 1148 245 2120
Fragments arranged by size:
2120 1148 1041 677 596 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5275
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 2188 3185 4730 1132
Size: 97 959 997 1545 2229
Fragments arranged by size:
2229 1545 997 959 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2999 1108
Size: 1891 3936
PfoI T'CCnGG_A
Cuts at: 2808 3007 2808
Size: 199 5628
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspOMI G'GGCC_C
Cuts at: 2127 2127
Size: 5827
PstI C_TGCA'G
Cuts at: 830 3179 830
Size: 2349 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SexAI A'CCwGG_T
Cuts at: 2495 2495
Size: 5827
SfcI C'TryA_G
Cuts at: 826 1045 2968 3175 4310 4991 5182 826
Size: 219 1923 207 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 219 207 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3931 4832 5094 820
Size: 197 926 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 2785 3751 5
Size: 47 2733 966 2081
Fragments arranged by size:
2733 2081 966 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 3266 513
Size: 552 2201 3074
Fragments arranged by size:
3074 2201 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 3871 4024 4209 4654 325
Size: 1255 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4073 5785 96
Size: 274 80 33 51 1443 2096 1712 138
Fragments arranged by size:
2096 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3015 3036 3047 3068 5787 5819 3015
Size: 21 11 21 2719 32 3023
Fragments arranged by size:
3023 2719 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 2603 2804 3956 2603
Size: 201 1152 4474
Fragments arranged by size:
4474 1152 201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI