Restriction map for
pFN21AB8977
(Circular) (Six-base) MAPSORT of: pFN21AB8977.seq Check: 5337 from: 1 to: 5824
pFN21AB8977 5824 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 2772 3145 2772
Size: 373 5451
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 3066 3887 5271 5539 1632
Size: 411 1023 821 1384 268 1917
Fragments arranged by size:
1917 1384 1023 821 411 268
AfeI AGC'GCT
Cuts at: 2799 2799
Size: 5824
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3084 1184
Size: 1900 3924
AlwNI CAG_nnn'CTG
Cuts at: 2122 2225 2498 2600 2636 3567 5139 2122
Size: 103 273 102 36 931 1572 2807
Fragments arranged by size:
2807 1572 931 273 103 102 36
ApaI G_GGCC'C
Cuts at: 2938 2938
Size: 5824
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 3096 3149 1870
Size: 73 1153 53 4545
Fragments arranged by size:
4545 1153 73 53
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2036 2069 2036
Size: 33 5791
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2772 3145 4600 618
Size: 325 217 627 985 373 1455 1842
Fragments arranged by size:
1842 1455 985 627 373 325 217
BanII G_rGCy'C
Cuts at: 1391 2154 2259 2625 2938 3143 1391
Size: 763 105 366 313 205 4072
Fragments arranged by size:
4072 763 366 313 205 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2430 928
Size: 971 531 4322
Fragments arranged by size:
4322 971 531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2343 2404 4435
Size: 107 122 71 631 605 671 61 2031
Cuts at: 4435 136
Size: 1525
Fragments arranged by size:
2031 1525 671 631 605 122 107 71
61
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 2646 3241 2646
Size: 595 5229
Bme1580I G_kGCm'C
Cuts at: 1790 2938 3096 3885 5041 1790
Size: 1148 158 789 1156 2573
Fragments arranged by size:
2573 1156 1148 789 158
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2528 3555 4513
Size: 416 53 83 399 24 1111 1027 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1111 1027 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2798 1056
Size: 1742 4082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2906 2918 2981 4484 1239
Size: 1265 402 12 63 1503 2579
Fragments arranged by size:
2579 1503 1265 402 63 12
Bpu10I CC'TnA_GC
Cuts at: 2374 2412 2374
Size: 38 5786
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 3949 4814 5112 2498
Size: 1451 865 298 3210
Fragments arranged by size:
3210 1451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 4487 882
Size: 617 1098 1890 2219
Fragments arranged by size:
2219 1890 1098 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2952 4257 4655 4929 5076 5487 2952
Size: 1305 398 274 147 411 3289
Fragments arranged by size:
3289 1305 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 4888
Fragments arranged by size:
4888 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2617 1186
Size: 1431 4393
BseYI C'CCAG_C
Cuts at: 2022 2161 2260 5027 5523 2022
Size: 139 99 2767 496 2323
Fragments arranged by size:
2767 2323 496 139 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2640 2900 1163
Size: 573 904 260 4087
Fragments arranged by size:
4087 904 573 260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 3885 3970 5041 2259
Size: 1626 85 1071 3042
Fragments arranged by size:
3042 1626 1071 85
BsmI GAATG_Cn'
Cuts at: 2131 3335 3428 2131
Size: 1204 93 4527
Fragments arranged by size:
4527 1204 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2259 2625 2938 3096 3143 3885
Size: 399 364 105 366 313 158 47 742
Cuts at: 3885 3970 5041 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 364 313 158
105 85 47
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3179 844
Size: 2335 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 2890 2902 2923 3711 1394
Size: 429 539 528 12 21 788 3507
Fragments arranged by size:
3507 788 539 528 429 21 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2740 4468 4655 1700
Size: 110 930 1728 187 2869
Fragments arranged by size:
2869 1728 930 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5824
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2569 2569
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 2284 3154 3521 3906 3923 5375 5461 5473
Size: 312 870 367 385 17 1452 86 12
Cuts at: 5473 5819 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 870 385 367 346 312 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2629 2629
Size: 5824
BtgI C'CryG_G
Cuts at: 513 1065 2938 513
Size: 552 1873 3399
Fragments arranged by size:
3399 1873 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2478 487
Size: 683 56 1252 3833
Fragments arranged by size:
3833 1252 683 56
BtsI GCAGTG_nn'
Cuts at: 2652 3340 4134 4154 2652
Size: 688 794 20 4322
Fragments arranged by size:
4322 794 688 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2729 4831 809
Size: 507 1413 2102 1802
Fragments arranged by size:
2102 1802 1413 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2310 2697 3271
Size: 54 1425 50 275 18 480 387 574
Cuts at: 3271 4160 5789 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 574 480 387 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3042 3754 1353
Size: 1689 712 3423
Fragments arranged by size:
3423 1689 712
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2257 2257
Size: 5824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2504 2906 2918 2981 3066 3887
Size: 393 411 461 402 12 63 85 821
Cuts at: 3887 4484 5271 5539 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 821 787 597 461 411 402 393
268 85 63 12
EcoO109I rG'GnC_Cy
Cuts at: 2633 2934 2633
Size: 301 5523
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2801 4971 1678
Size: 1123 2170 2531
Fragments arranged by size:
2531 2170 1123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2417 2436 2449
Size: 32 135 32 512 32 301 19 13
Cuts at: 2449 2456 2468 2488 4465 4497 4539 4571 1373
Size: 7 12 20 1977 32 42 32 2626
Fragments arranged by size:
2626 1977 512 301 135 42 32 32
32 32 32 20 19 13 12 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 3168 3414 669
Size: 988 281 796 434 246 3079
Fragments arranged by size:
3079 988 796 434 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 2776 3149 2776
Size: 373 5451
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 2604 2839 4938 5122 1305
Size: 1155 144 235 2099 184 2007
Fragments arranged by size:
2099 2007 1155 235 184 144
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2312 2699 10
Size: 54 1425 343 480 387 3135
Fragments arranged by size:
3135 1425 480 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3782 4141 4300 518
Size: 648 2616 359 159 2042
Fragments arranged by size:
2616 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2597 2779 2884 2940 2980 3240 3917
Size: 444 547 182 105 56 40 260 677
Cuts at: 3917 5065 5310 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 547 444 260 245 182
105 56 40
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5272
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 2794 1052
Size: 1742 4082
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 3182 4727 1132
Size: 97 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2174 2225 1108
Size: 1066 51 4707
Fragments arranged by size:
4707 1066 51
PfoI T'CCnGG_A
Cuts at: 2382 2805 2382
Size: 423 5401
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2934 2934
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2118 3176 830
Size: 1288 1058 3478
Fragments arranged by size:
3478 1288 1058
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2050 2597 2884 2980 2050
Size: 547 287 96 4894
Fragments arranged by size:
4894 547 287 96
SacI G_AGCT'C
Cuts at: 2259 2259
Size: 5824
SacII CC_GC'GG
Cuts at: 2941 2941
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2114 2458 3172 4307 4988 5179 826
Size: 219 1069 344 714 1135 681 191 1471
Fragments arranged by size:
1471 1135 1069 714 681 344 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3098 3151 1872
Size: 1226 53 4545
Fragments arranged by size:
4545 1226 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 3928 4829 5091 820
Size: 197 926 534 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 534 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2346 1517
Size: 829 4995
StyI C'CwwG_G
Cuts at: 513 1065 2173 2568 2610 2694 3263 513
Size: 552 1108 395 42 84 569 3074
Fragments arranged by size:
3074 1108 569 552 395 84 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2203 2232 3851 3868 4021 4206 4651
Size: 1255 623 29 1619 17 153 185 445
Cuts at: 4651 325
Size: 1498
Fragments arranged by size:
1619 1498 1255 623 445 185 153 29
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4070 5782 96
Size: 274 80 33 51 1443 2093 1712 138
Fragments arranged by size:
2093 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2881 2222
Size: 659 5165
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3096 3149 1870
Size: 1226 53 4545
Fragments arranged by size:
4545 1226 53
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI