Restriction map for
pFN21AB8976
(Circular) (Six-base) MAPSORT of: pFN21AB8976.seq Check: 4307 from: 1 to: 5821
pFN21AB8976 5821 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2885 3884 5268 5536 1632
Size: 1253 999 1384 268 1917
Fragments arranged by size:
1917 1384 1253 999 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2432 1184
Size: 1248 4573
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 2039
Size: 32 5789
AlwNI CAG_nnn'CTG
Cuts at: 3564 5136 3564
Size: 1572 4249
ApaI G_GGCC'C
Cuts at: 2136 2136
Size: 5821
ApaLI G'TGCA_C
Cuts at: 2729 3878 5034 2729
Size: 1149 1156 3516
Fragments arranged by size:
3516 1156 1149
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 3146 1870
Size: 73 93 1110 4545
Fragments arranged by size:
4545 1110 93 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 2754 3142 4597 618
Size: 325 217 627 451 516 388 1455 1842
Fragments arranged by size:
1842 1455 627 516 451 388 325 217
BanII G_rGCy'C
Cuts at: 1391 2136 2339 3140 1391
Size: 745 203 801 4072
Fragments arranged by size:
4072 801 745 203
BbeI G_GCGC'C
Cuts at: 2758 2758
Size: 5821
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 928
Size: 971 400 4450
Fragments arranged by size:
4450 971 400
BbvCI CC'TCA_GC
Cuts at: 2760 2760
Size: 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2136 2169 2733 3882 5038 1790
Size: 346 33 564 1149 1156 2573
Fragments arranged by size:
2573 1156 1149 564 346 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2252 4481 1239
Size: 827 186 2229 2579
Fragments arranged by size:
2579 2229 827 186
Bpu10I CC'TnA_GC
Cuts at: 2760 2760
Size: 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 4484 882
Size: 617 759 2226 2219
Fragments arranged by size:
2226 2219 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 2719 3612 493
Size: 711 1515 893 2702
Fragments arranged by size:
2702 1515 893 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2755 3628 4010 4645
Size: 53 83 186 936 1222 873 382 635
Cuts at: 4645 275
Size: 1451
Fragments arranged by size:
1451 1222 936 873 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2078 2108 2579
Size: 30 165 30 636 30 9 30 471
Cuts at: 2579 2609 1178
Size: 30 4390
Fragments arranged by size:
4390 636 471 165 30 30 30 30
30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 1186
Size: 1297 4524
BseYI C'CCAG_C
Cuts at: 2346 2748 3022 5024 5520 2346
Size: 402 274 2002 496 2647
Fragments arranged by size:
2647 2002 496 402 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 1163
Size: 573 636 4612
Fragments arranged by size:
4612 636 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2733 3882 3967 5038 2733
Size: 1149 85 1071 3516
Fragments arranged by size:
3516 1149 1071 85
BsmI GAATG_Cn'
Cuts at: 3103 3332 3425 3103
Size: 229 93 5499
Fragments arranged by size:
5499 229 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2169 2339 2733 3140 3882 3967
Size: 399 346 33 170 394 407 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 407 399 394 346 170
85 33
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 3176 844
Size: 2047 285 3489
Fragments arranged by size:
3489 2047 285
BsrBI CCG'CTC
Cuts at: 1394 1823 2236 3708 1394
Size: 429 413 1472 3507
Fragments arranged by size:
3507 1472 429 413
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2241 2978 3032 4465 4652 1700
Size: 110 431 737 54 1433 187 2869
Fragments arranged by size:
2869 1433 737 431 187 110 54
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2737 794
Size: 1943 3878
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 2820 1184
Size: 1636 4185
BstXI CCAn_nnnn'nTGG
Cuts at: 2527 2527
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816
Size: 1179 367 385 17 1452 86 12 346
Cuts at: 5816 1972
Size: 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2169 2259 2286 2325 2436 2871 2907
Size: 552 1104 90 27 39 111 435 36
Cuts at: 2907 513
Size: 3427
Fragments arranged by size:
3427 1104 552 435 111 90 39 36
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2416 487
Size: 683 56 1190 3892
Fragments arranged by size:
3892 1190 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3001 3337 4131 4151 2944
Size: 57 336 794 20 4614
Fragments arranged by size:
4614 794 336 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DraIII CAC_nnn'GTG
Cuts at: 2922 2922
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2289 2328
Size: 54 1425 50 275 18 453 6 39
Cuts at: 2328 2451 2784 2910 3268 4157 5786 8
Size: 123 333 126 358 889 1629 43
Fragments arranged by size:
1629 1425 889 453 358 333 275 126
123 54 50 43 39 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2567 3062 3751 1353
Size: 1214 495 689 3423
Fragments arranged by size:
3423 1214 689 495
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2252 2885 3884 4481 5268 5536
Size: 393 434 186 633 999 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 999 787 633 597 434 393 268
186
EcoO109I rG'GnC_Cy
Cuts at: 2050 2132 2133 2350 2358 2050
Size: 82 1 217 8 5513
Fragments arranged by size:
5513 217 82 8 1
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2758 4968 1678
Size: 1080 2210 2531
Fragments arranged by size:
2531 2210 1080
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4462 4494 4536 4568 1373
Size: 32 135 32 2890 32 42 32 2626
Fragments arranged by size:
2890 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2398 3165 3411 669
Size: 988 281 460 767 246 3079
Fragments arranged by size:
3079 988 767 460 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KasI G'GCGC_C
Cuts at: 2754 2754
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4935 5119 1305
Size: 3630 184 2007
Fragments arranged by size:
3630 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 2786 2912 10
Size: 54 1425 343 621 333 126 2919
Fragments arranged by size:
2919 1425 621 343 333 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2699 2792 2918 3779 4138 4297
Size: 648 1269 264 93 126 861 359 159
Cuts at: 4297 518
Size: 2042
Fragments arranged by size:
2042 1269 861 648 359 264 159 126
93
MspA1I CmG'CkG
Cuts at: 1606 2752 2764 3237 3914 5062 5307 1606
Size: 1146 12 473 677 1148 245 2120
Fragments arranged by size:
2120 1148 1146 677 473 245 12
NaeI GCC'GGC
Cuts at: 2980 2980
Size: 5821
NarI GG'CG_CC
Cuts at: 2755 2755
Size: 5821
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2978 2978
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2645 3179 4724 1132
Size: 97 1416 534 1545 2229
Fragments arranged by size:
2229 1545 1416 534 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PfoI T'CCnGG_A
Cuts at: 2229 2844 2229
Size: 615 5206
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2071 3890 3922 5403 5435 2039
Size: 32 1819 32 1481 32 2425
Fragments arranged by size:
2425 1819 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2350 2358 2050
Size: 300 8 5513
Fragments arranged by size:
5513 300 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2132 2132
Size: 5821
PstI C_TGCA'G
Cuts at: 830 2411 3173 830
Size: 1581 762 3478
Fragments arranged by size:
3478 1581 762
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 3062 3062
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2176 2407 3169 4304 4985 5176 826
Size: 219 1131 231 762 1135 681 191 1471
Fragments arranged by size:
1471 1135 1131 762 681 231 219 191
SfoI GGC'GCC
Cuts at: 2756 2756
Size: 5821
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 2978 1700
Size: 1278 4543
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 3925 4826 5088 820
Size: 197 926 93 1889 901 262 1553
Fragments arranged by size:
1889 1553 926 901 262 197 93
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 2645 3179 1132
Size: 1513 534 3774
Fragments arranged by size:
3774 1513 534
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2578 3260 513
Size: 552 1513 682 3074
Fragments arranged by size:
3074 1513 682 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4067 5779 96
Size: 274 80 33 51 1443 2090 1712 138
Fragments arranged by size:
2090 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 5781 5813 2579
Size: 32 3170 32 2587
Fragments arranged by size:
3170 2587 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2510 2527 2701 2510
Size: 17 174 5630
Fragments arranged by size:
5630 174 17
XhoI C'TCGA_G
Cuts at: 1943 2036 1943
Size: 93 5728
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI