Restriction map for
pFN21AB8968
(Circular) (Six-base) MAPSORT of: pFN21AB8968.seq Check: 943 from: 1 to: 5776
pFN21AB8968 5776 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2254 2254
Size: 5776
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3027 3119 1937
Size: 1090 92 4594
Fragments arranged by size:
4594 1090 92
Acc65I G'GTAC_C
Cuts at: 2355 3097 2355
Size: 742 5034
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2421 3839 5223 5491 1632
Size: 789 1418 1384 268 1917
Fragments arranged by size:
1917 1418 1384 789 268
AfeI AGC'GCT
Cuts at: 2339 2753 2339
Size: 414 5362
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2328 1184
Size: 1144 4632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 2078
Size: 32 5744
AlwNI CAG_nnn'CTG
Cuts at: 3004 3519 5091 3004
Size: 515 1572 3689
Fragments arranged by size:
3689 1572 515
ApaI G_GGCC'C
Cuts at: 2233 2983 2233
Size: 750 5026
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2729 3101 1870
Size: 73 72 714 372 4545
Fragments arranged by size:
4545 714 372 73 72
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2240 2355 2578 2920
Size: 325 217 627 246 207 115 223 342
Cuts at: 2920 2986 3097 4552 618
Size: 66 111 1455 1842
Fragments arranged by size:
1842 1455 627 342 325 246 223 217
207 115 111 66
BanII G_rGCy'C
Cuts at: 1391 2233 2519 2983 3095 1391
Size: 842 286 464 112 4072
Fragments arranged by size:
4072 842 464 286 112
BbeI G_GCGC'C
Cuts at: 2244 2582 2244
Size: 338 5438
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 2695 2696 2713 928
Size: 971 741 55 1 17 3991
Fragments arranged by size:
3991 971 741 55 17 1
BbvCI CC'TCA_GC
Cuts at: 2148 2148
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3967 4001 1903
Size: 34 50 34 1946 34 3678
Fragments arranged by size:
3678 1946 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2219 2228 2783
Size: 107 122 71 631 605 547 9 555
Cuts at: 2783 2978 4387 136
Size: 195 1409 1525
Fragments arranged by size:
1525 1409 631 605 555 547 195 122
107 71 9
BglII A'GATC_T
Cuts at: 2820 5771 2820
Size: 2951 2825
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2233 2925 2983 3837 4993 1790
Size: 443 692 58 854 1156 2573
Fragments arranged by size:
2573 1156 854 692 443 58
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2563 2524
Size: 39 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2354 2555 2894 4436 1239
Size: 1016 99 201 339 1542 2579
Fragments arranged by size:
2579 1542 1016 339 201 99
Bpu10I CC'TnA_GC
Cuts at: 2142 2148 2888 2142
Size: 6 740 5030
Fragments arranged by size:
5030 740 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 3901 4766 5064 2462
Size: 1439 865 298 3174
Fragments arranged by size:
3174 1439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2115 3052 4439 882
Size: 617 616 937 1387 2219
Fragments arranged by size:
2219 1387 937 617 616
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 2579 3583 3965
Size: 53 83 186 936 708 338 1004 382
Cuts at: 3965 4600 275
Size: 635 1451
Fragments arranged by size:
1451 1004 936 708 635 382 338 186
83 53
BsaWI w'CCGG_w
Cuts at: 2464 4209 4607 4881 5028 5439 2464
Size: 1745 398 274 147 411 2801
Fragments arranged by size:
2801 1745 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 1178
Size: 30 165 30 675 30 4846
Fragments arranged by size:
4846 675 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2422 2552 1186
Size: 1236 130 4410
Fragments arranged by size:
4410 1236 130
BseYI C'CCAG_C
Cuts at: 2344 4979 5475 2344
Size: 2635 496 2645
Fragments arranged by size:
2645 2635 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2780 1163
Size: 573 1044 4159
Fragments arranged by size:
4159 1044 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2135 2592 2952 3071 3075 3226
Size: 275 186 134 457 360 119 4 151
Cuts at: 3226 3987 5015 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 457 360 275 186 151
134 119 4
BsiHKAI G_wGCw'C
Cuts at: 3837 3922 4993 3837
Size: 85 1071 4620
Fragments arranged by size:
4620 1071 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
BsmBI CGTCTCn'nnnn_
Cuts at: 2801 2801
Size: 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2519 2925 2983 3095 3837 3922
Size: 399 443 286 406 58 112 742 85
Cuts at: 3922 4993 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 443 406 399 286 112
85 58
BspEI T'CCGG_A
Cuts at: 2464 5439 2464
Size: 2975 2801
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 2254 2803 3131 844
Size: 1274 136 549 328 3489
Fragments arranged by size:
3489 1274 549 328 136
BsrBI CCG'CTC
Cuts at: 1394 1823 2078 3663 1394
Size: 429 255 1585 3507
Fragments arranged by size:
3507 1585 429 255
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2130 2378 2671 2777 3070 4420
Size: 110 220 100 248 293 106 293 1350
Cuts at: 4420 4607 1700
Size: 187 2869
Fragments arranged by size:
2869 1350 293 293 248 220 187 110
106 100
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 2936 3836 4848 2936
Size: 900 1012 3864
Fragments arranged by size:
3864 1012 900
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2352 2352
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2295 2820 3106 3473 3858 3875 5327 5413
Size: 323 525 286 367 385 17 1452 86
Cuts at: 5413 5425 5771 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 525 385 367 346 323 286
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2159 2214 2244 2898 2973 513
Size: 552 1094 55 30 654 75 3316
Fragments arranged by size:
3316 1094 654 552 75 55 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2471 3292 4086 4106 2471
Size: 821 794 20 4141
Fragments arranged by size:
4141 821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DraIII CAC_nnn'GTG
Cuts at: 3043 3043
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 2220 2383
Size: 54 1425 50 275 18 302 88 163
Cuts at: 2383 2589 2616 2784 2880 2949 3068 3223 4112
Size: 206 27 168 96 69 119 155 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 302 275 206 168 163
155 119 96 88 69 54 50 43
27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2132 2589 2949 3068 3223 1537
Size: 275 320 457 360 119 155 4090
Fragments arranged by size:
4090 457 360 320 275 155 119
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 3055 3706 1353
Size: 821 881 651 3423
Fragments arranged by size:
3423 881 821 651
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2882 4363 4737 4883 387
Size: 1064 138 1293 1481 374 146 1280
Fragments arranged by size:
1481 1293 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2354 2421 2555 2894 3839 4436
Size: 393 623 99 67 134 339 945 597
Cuts at: 4436 5223 5491 1239
Size: 787 268 1524
Fragments arranged by size:
1524 945 787 623 597 393 339 268
134 99 67
EcoO109I rG'GnC_Cy
Cuts at: 2229 2230 2979 2980 2229
Size: 1 749 1 5025
Fragments arranged by size:
5025 749 1 1
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2244 2341 2582 2652 2755 4923 1678
Size: 566 97 241 70 103 2168 2531
Fragments arranged by size:
2531 2168 566 241 103 97 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 4417 4449 4491
Size: 32 135 32 872 32 1941 32 42
Cuts at: 4491 4523 1373
Size: 32 2626
Fragments arranged by size:
2626 1941 872 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2240 2578 2240
Size: 338 5438
KpnI G_GTAC'C
Cuts at: 2359 3101 2359
Size: 742 5034
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3734 4093 4252 518
Size: 648 2568 359 159 2042
Fragments arranged by size:
2568 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 2210 2216 2246 2860 2900 2975 3004
Size: 598 6 6 30 614 40 75 29
Cuts at: 3004 3061 3192 3869 5017 5262 1606
Size: 57 131 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 614 598 245 131 75
57 40 30 29 6 6
NaeI GCC'GGC
Cuts at: 2032 2380 2673 2779 2032
Size: 348 293 106 5029
Fragments arranged by size:
5029 348 293 106
NarI GG'CG_CC
Cuts at: 2241 2579 2241
Size: 338 5438
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NgoMIV G'CCGG_C
Cuts at: 2030 2378 2671 2777 2030
Size: 348 293 106 5029
Fragments arranged by size:
5029 348 293 106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2078 2110 3845 3877 5358 5390 2078
Size: 32 1735 32 1481 32 2464
Fragments arranged by size:
2464 1735 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2813 1613
Size: 1200 4576
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2229 2979 2229
Size: 750 5026
PspXI vC'TCGA_Gb
Cuts at: 2729 2729
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2971 3128 830
Size: 2141 157 3478
Fragments arranged by size:
3478 2141 157
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2860 2860
Size: 5776
SacII CC_GC'GG
Cuts at: 2217 2247 2901 2976 2217
Size: 30 654 75 5017
Fragments arranged by size:
5017 654 75 30
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2174 2174
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2967 3124 4259 4940 5131 826
Size: 219 1922 157 1135 681 191 1471
Fragments arranged by size:
1922 1471 1135 681 219 191 157
SfiI GGCCn_nnn'nGGCC
Cuts at: 2228 2228
Size: 5776
SfoI GGC'GCC
Cuts at: 2242 2580 2242
Size: 338 5438
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 2378 1700
Size: 678 5098
SmaI CCC'GGG
Cuts at: 1872 2017 3103 1872
Size: 145 1086 4545
Fragments arranged by size:
4545 1086 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 2729 3880 4781 5043 820
Size: 197 926 534 252 1151 901 262 1553
Fragments arranged by size:
1553 1151 926 901 534 262 252 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2417 1517
Size: 900 4876
StyI C'CwwG_G
Cuts at: 513 1065 2082 2223 3215 513
Size: 552 1017 141 992 3074
Fragments arranged by size:
3074 1017 992 552 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2230
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 2729 1943
Size: 786 4990
XmaI C'CCGG_G
Cuts at: 1870 2015 3101 1870
Size: 145 1086 4545
Fragments arranged by size:
4545 1086 145
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PsrI RsrII SacI SanDI SexAI
SrfI SwaI