Restriction map for
pFN21AB8965
(Circular) (Six-base) MAPSORT of: pFN21AB8965.seq Check: 7015 from: 1 to: 5767
pFN21AB8965 5767 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2583 2583
Size: 5767
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2715 3110 1937
Size: 778 395 4594
Fragments arranged by size:
4594 778 395
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3830 5214 5482 1632
Size: 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 2014 4496 2014
Size: 2482 3285
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2567 1184
Size: 1383 4384
AlwNI CAG_nnn'CTG
Cuts at: 2161 2797 3510 5082 2161
Size: 636 713 1572 2846
Fragments arranged by size:
2846 1572 713 636
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2719 3092 1870
Size: 73 776 373 4545
Fragments arranged by size:
4545 776 373 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2061 2566 2656 2684 2771
Size: 325 217 627 274 505 90 28 87
Cuts at: 2771 3088 4543 618
Size: 317 1455 1842
Fragments arranged by size:
1842 1455 627 505 325 317 274 217
90 87 28
BanII G_rGCy'C
Cuts at: 1391 2192 2336 2412 2453 2726 3017 3086 1391
Size: 801 144 76 41 273 291 69 4072
Fragments arranged by size:
4072 801 291 273 144 76 69 41
BbeI G_GCGC'C
Cuts at: 2065 2688 2775 2065
Size: 623 87 5057
Fragments arranged by size:
5057 623 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 2956 928
Size: 971 149 908 3739
Fragments arranged by size:
3739 971 908 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2352 2386 3958 3992 1903
Size: 34 415 34 1572 34 3678
Fragments arranged by size:
3678 1572 415 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2148 5762 2148
Size: 3614 2153
BlpI GC'TnA_GC
Cuts at: 2141 3184 2141
Size: 1043 4724
Bme1580I G_kGCm'C
Cuts at: 1790 2474 2571 2661 3828 4984 1790
Size: 684 97 90 1167 1156 2573
Fragments arranged by size:
2573 1167 1156 684 97 90
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 2876 4427 1239
Size: 1274 363 1551 2579
Fragments arranged by size:
2579 1551 1274 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 3892 4757 5055 2366
Size: 1526 865 298 3078
Fragments arranged by size:
3078 1526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2062 2685 2750 2772
Size: 53 83 186 936 529 623 65 22
Cuts at: 2772 2984 3574 3956 4591 275
Size: 212 590 382 635 1451
Fragments arranged by size:
1451 936 635 623 590 529 382 212
186 83 65 53 22
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2849 2879 1178
Size: 30 165 30 1446 30 4066
Fragments arranged by size:
4066 1446 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 1186
Size: 1156 4611
BseYI C'CCAG_C
Cuts at: 2555 2893 2936 4970 5466 2555
Size: 338 43 2034 496 2856
Fragments arranged by size:
2856 2034 496 338 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2855 1163
Size: 573 1119 4075
Fragments arranged by size:
4075 1119 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2351 2457 2628 3006 3217 3978
Size: 275 186 350 106 171 378 211 761
Cuts at: 3978 5006 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 378 350 275 211 186
171 106
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2336 2412 2453 2474 2571 2661
Size: 399 402 144 76 41 21 97 90
Cuts at: 2661 2726 3017 3086 3828 3913 4984 1391
Size: 65 291 69 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 291 144 97
90 85 76 69 65 41 21
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2583 3022 3122 844
Size: 1739 439 100 3489
Fragments arranged by size:
3489 1739 439 100
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2456 2627 2681 2687 3005 4411
Size: 110 241 405 171 54 6 318 1406
Cuts at: 4411 4598 1700
Size: 187 2869
Fragments arranged by size:
2869 1406 405 318 241 187 171 110
54 6
BsrGI T'GTAC_A
Cuts at: 96 2391 96
Size: 2295 3472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 2467 3827 4839 2467
Size: 1360 1012 3395
Fragments arranged by size:
3395 1360 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 3034 1184
Size: 1850 3917
BstXI CCAn_nnnn'nTGG
Cuts at: 2485 2485
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2148 3097 3464 3849 3866 5318 5404 5416
Size: 176 949 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 949 385 367 346 176 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2754 2814 2915 2979 513
Size: 552 939 750 60 101 64 3301
Fragments arranged by size:
3301 939 750 552 101 64 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2074 487
Size: 683 56 848 4180
Fragments arranged by size:
4180 848 683 56
BtsI GCAGTG_nn'
Cuts at: 3283 4077 4097 3283
Size: 794 20 4953
Fragments arranged by size:
4953 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DraIII CAC_nnn'GTG
Cuts at: 2566 2566
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2307 2348
Size: 54 1425 50 275 18 276 201 41
Cuts at: 2348 2454 2518 2590 2625 2738 2817 3003 3214
Size: 106 64 72 35 113 79 186 211
Cuts at: 3214 4103 5732 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 276 275 211 201 186
113 106 79 72 64 54 50 43
41 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2348 2454 2625 3003 3214 1537
Size: 275 536 106 171 378 211 4090
Fragments arranged by size:
4090 536 378 275 211 171 106
EarI CTCTTCn'nnn_
Cuts at: 1353 2995 3697 1353
Size: 1642 702 3423
Fragments arranged by size:
3423 1642 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2876 3830 4427 5214 5482 1239
Size: 393 881 363 954 597 787 268 1524
Fragments arranged by size:
1524 954 881 787 597 393 363 268
EcoO109I rG'GnC_Cy
Cuts at: 2263 2263
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2065 2134 2381 2492 2688 2762 2775 4914
Size: 387 69 247 111 196 74 13 2139
Cuts at: 4914 1678
Size: 2531
Fragments arranged by size:
2531 2139 387 247 196 111 74 69
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 4408 4440 4482
Size: 32 135 32 428 32 2376 32 42
Cuts at: 4482 4514 1373
Size: 32 2626
Fragments arranged by size:
2626 2376 428 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KasI G'GCGC_C
Cuts at: 2061 2684 2771 2061
Size: 623 87 5057
Fragments arranged by size:
5057 623 87
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 4881 5065 1305
Size: 1302 2274 184 2007
Fragments arranged by size:
2274 2007 1302 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 10
Size: 54 1425 343 276 3669
Fragments arranged by size:
3669 1425 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 3725 4084 4243 518
Size: 648 936 1623 359 159 2042
Fragments arranged by size:
2042 1623 936 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2671 2756 2769 2816 2917 3183 3860 5008
Size: 1065 85 13 47 101 266 677 1148
Cuts at: 5008 5253 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1065 677 266 245 101 85
47 13
NaeI GCC'GGC
Cuts at: 2053 2458 2629 3007 2053
Size: 405 171 378 4813
Fragments arranged by size:
4813 405 378 171
NarI GG'CG_CC
Cuts at: 2062 2685 2772 2062
Size: 623 87 5057
Fragments arranged by size:
5057 623 87
NcoI C'CATG_G
Cuts at: 513 1065 2004 2979 513
Size: 552 939 975 3301
Fragments arranged by size:
3301 975 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NgoMIV G'CCGG_C
Cuts at: 2051 2456 2627 3005 2051
Size: 405 171 378 4813
Fragments arranged by size:
4813 405 378 171
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 2348 3214 2348
Size: 866 4901
NspI r_CATG'y
Cuts at: 1132 1229 2944 3125 4670 1132
Size: 97 1715 181 1545 2229
Fragments arranged by size:
2229 1715 1545 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PfoI T'CCnGG_A
Cuts at: 2427 2427
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2306 2338 3836 3868 5349 5381 2306
Size: 32 1498 32 1481 32 2692
Fragments arranged by size:
2692 1498 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2166 3119 830
Size: 1336 953 3478
Fragments arranged by size:
3478 1336 953
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
SacII CC_GC'GG
Cuts at: 2757 2817 2918 2757
Size: 60 101 5606
Fragments arranged by size:
5606 101 60
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2162 3115 4250 4931 5122 826
Size: 219 1117 953 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 953 681 219 191
SfoI GGC'GCC
Cuts at: 2063 2686 2773 2063
Size: 623 87 5057
Fragments arranged by size:
5057 623 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 2681 2687 1700
Size: 981 6 4780
Fragments arranged by size:
4780 981 6
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2381 3871 4772 5034 820
Size: 197 926 438 1490 901 262 1553
Fragments arranged by size:
1553 1490 926 901 438 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2259 2266 2979 3206 513
Size: 552 939 27 228 7 713 227 3074
Fragments arranged by size:
3074 939 713 552 228 227 27 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 2964 3794 3811 3964 4149 4594
Size: 1255 557 827 830 17 153 185 445
Cuts at: 4594 325
Size: 1498
Fragments arranged by size:
1498 1255 830 827 557 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 4013 5725
Size: 274 80 33 51 1443 414 1622 1712
Cuts at: 5725 96
Size: 138
Fragments arranged by size:
1712 1622 1443 414 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2564 2596 5727 5759 2564
Size: 32 3131 32 2572
Fragments arranged by size:
3131 2572 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2851 2851
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SrfI SwaI