Restriction map for
pFN21AB8964
(Circular) (Six-base) MAPSORT of: pFN21AB8964.seq Check: 7578 from: 1 to: 5755
pFN21AB8964 5755 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2221 2221
Size: 5755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2076 3012 3098 1937
Size: 139 936 86 4594
Fragments arranged by size:
4594 936 139 86
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2900 3012 3818 5202 5470 1632
Size: 1268 112 806 1384 268 1917
Fragments arranged by size:
1917 1384 1268 806 268 112
AfeI AGC'GCT
Cuts at: 2483 2483
Size: 5755
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2601 4654 5631 1203
Size: 1398 2053 977 1327
Fragments arranged by size:
2053 1398 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2213 2711 2777 3498 5070 2213
Size: 498 66 721 1572 2898
Fragments arranged by size:
2898 1572 721 498 66
ApaI G_GGCC'C
Cuts at: 2232 2287 2581 2232
Size: 55 294 5406
Fragments arranged by size:
5406 294 55
ApaLI G'TGCA_C
Cuts at: 2303 3812 4968 2303
Size: 1509 1156 3090
Fragments arranged by size:
3090 1509 1156
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 3080 1870
Size: 73 344 793 4545
Fragments arranged by size:
4545 793 344 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 2401 3076 4531 618
Size: 325 217 627 413 201 675 1455 1842
Fragments arranged by size:
1842 1455 675 627 413 325 217 201
BanII G_rGCy'C
Cuts at: 1391 2015 2169 2232 2287 2570 2581 2815 2868
Size: 624 154 63 55 283 11 234 53
Cuts at: 2868 3074 1391
Size: 206 4072
Fragments arranged by size:
4072 624 283 234 206 154 63 55
53 11
BbeI G_GCGC'C
Cuts at: 2405 2405
Size: 5755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3946 3980 1903
Size: 34 50 34 1925 34 3678
Fragments arranged by size:
3678 1925 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BfrBI ATG'CAT
Cuts at: 3050 3050
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2232 2287 2307 2581 3816 4972 1790
Size: 310 132 55 20 274 1235 1156 2573
Fragments arranged by size:
2573 1235 1156 310 274 132 55 20
BmgBI CAC'GTC
Cuts at: 1318 2968 1318
Size: 1650 4105
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2087 2584 3486
Size: 416 53 83 399 24 670 497 902
Cuts at: 3486 4444 442
Size: 958 1753
Fragments arranged by size:
1753 958 902 670 497 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 2754 2882 3027 4415 1239
Size: 1430 85 128 145 1388 2579
Fragments arranged by size:
2579 1430 1388 145 128 85
Bpu10I CC'TnA_GC
Cuts at: 2593 2778 2593
Size: 185 5570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2443 3880 4745 5043 2443
Size: 1437 865 298 3155
Fragments arranged by size:
3155 1437 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2402 3562 3944 4579
Size: 53 83 186 936 869 1160 382 635
Cuts at: 4579 275
Size: 1451
Fragments arranged by size:
1451 1160 936 869 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 1178
Size: 30 165 30 1497 30 4003
Fragments arranged by size:
4003 1497 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 1186
Size: 1480 4275
BseYI C'CCAG_C
Cuts at: 2008 2206 2588 2757 4958 5454 2008
Size: 198 382 169 2201 496 2309
Fragments arranged by size:
2309 2201 496 382 198 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2868 3816 3901 4972 2307
Size: 561 948 85 1071 3090
Fragments arranged by size:
3090 1071 948 561 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2100 2169 2232 2287 2307 2570
Size: 399 225 85 69 63 55 20 263
Cuts at: 2570 2581 2815 2868 3074 3816 3901 4972 1391
Size: 11 234 53 206 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 263 234 225 206
85 85 69 63 55 53 20 11
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2221 2631 3110 844
Size: 1377 410 479 3489
Fragments arranged by size:
3489 1377 479 410
BsrBI CCG'CTC
Cuts at: 1394 1823 3036 3642 1394
Size: 429 1213 606 3507
Fragments arranged by size:
3507 1213 606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2083 4399 4586 1700
Size: 110 273 2316 187 2869
Fragments arranged by size:
2869 2316 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 2086 1184
Size: 902 4853
BstYI r'GATC_y
Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750
Size: 1113 367 385 17 1452 86 12 346
Cuts at: 5750 1972
Size: 1977
Fragments arranged by size:
1977 1452 1113 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2188 2784 2188
Size: 596 5159
BtgI C'CryG_G
Cuts at: 513 1065 2103 2385 2733 513
Size: 552 1038 282 348 3535
Fragments arranged by size:
3535 1038 552 348 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2180 3271 4065 4085 2180
Size: 1091 794 20 3850
Fragments arranged by size:
3850 1091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2528 4762 809
Size: 507 1212 2234 1802
Fragments arranged by size:
2234 1802 1212 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2388 2736
Size: 54 1425 50 275 18 210 348 348
Cuts at: 2736 3202 4091 5720 8
Size: 466 889 1629 43
Fragments arranged by size:
1629 1425 889 466 348 348 275 210
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 3685 1353
Size: 1106 1226 3423
Fragments arranged by size:
3423 1226 1106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2866 2866
Size: 5755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2669 2754 2882 2900 3012 3027 3818
Size: 393 1037 85 128 18 112 15 791
Cuts at: 3818 4415 5202 5470 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1037 791 787 597 393 268 128
112 85 18 15
EcoO109I rG'GnC_Cy
Cuts at: 2192 2228 2283 2284 2291 2578 3022 2192
Size: 36 55 1 7 287 444 4925
Fragments arranged by size:
4925 444 287 55 36 7 1
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2405 2485 4902 1678
Size: 727 80 2417 2531
Fragments arranged by size:
2531 2417 727 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2119 2151 2810 2842 4396
Size: 32 135 32 547 32 659 32 1554
Cuts at: 4396 4428 4470 4502 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1554 659 547 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KasI G'GCGC_C
Cuts at: 2401 2401
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2390 10
Size: 54 1425 343 558 3375
Fragments arranged by size:
3375 1425 558 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 3713 4072 4231 518
Size: 648 1218 1329 359 159 2042
Fragments arranged by size:
2042 1329 1218 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2256 2676 2735 3171 3848 4996 5241 1606
Size: 650 420 59 436 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 650 436 420 245 59
NarI GG'CG_CC
Cuts at: 2402 2402
Size: 5755
NcoI C'CATG_G
Cuts at: 513 1065 2103 2385 513
Size: 552 1038 282 3883
Fragments arranged by size:
3883 1038 552 282
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NsiI A_TGCA'T
Cuts at: 3052 3052
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2605 3113 4658 1132
Size: 97 1376 508 1545 2229
Fragments arranged by size:
2229 1545 1376 508 97
PasI CC'CwG_GG
Cuts at: 1348 2159 2224 2791 2870 1348
Size: 811 65 567 79 4233
Fragments arranged by size:
4233 811 567 79 65
PciI A'CATG_T
Cuts at: 2601 4654 2601
Size: 2053 3702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2038 1108
Size: 930 4825
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2192 2291 2192
Size: 99 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2228 2283 2577 2228
Size: 55 294 5406
Fragments arranged by size:
5406 294 55
PstI C_TGCA'G
Cuts at: 830 2218 3107 830
Size: 1388 889 3478
Fragments arranged by size:
3478 1388 889
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SacI G_AGCT'C
Cuts at: 2868 2868
Size: 5755
SacII CC_GC'GG
Cuts at: 2736 2736
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2214 3103 4238 4919 5110 826
Size: 219 1169 889 1135 681 191 1471
Fragments arranged by size:
1471 1169 1135 889 681 219 191
SfoI GGC'GCC
Cuts at: 2403 2403
Size: 5755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 2083 1700
Size: 383 5372
SmaI CCC'GGG
Cuts at: 1872 2289 3082 1872
Size: 417 793 4545
Fragments arranged by size:
4545 793 417
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2422 3859 4760 5022 820
Size: 197 926 479 1437 901 262 1553
Fragments arranged by size:
1553 1437 926 901 479 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 2244 1517
Size: 727 5028
StyI C'CwwG_G
Cuts at: 513 1065 2103 2385 3194 513
Size: 552 1038 282 809 3074
Fragments arranged by size:
3074 1038 809 552 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2237 3782 3799 3952 4137 4582 325
Size: 1255 657 1545 17 153 185 445 1498
Fragments arranged by size:
1545 1498 1255 657 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2900 2932 5715 5747 2900
Size: 32 2783 32 2908
Fragments arranged by size:
2908 2783 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2707 1316
Size: 1391 4364
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2965 2965
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 2287 3080 1870
Size: 417 793 4545
Fragments arranged by size:
4545 793 417
XmnI GAAnn'nnTTC
Cuts at: 2639 3884 2639
Size: 1245 4510
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstXI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SanDI SapI SexAI SfiI SrfI SwaI