Restriction map for
pFN21AB8963
(Circular) (Six-base) MAPSORT of: pFN21AB8963.seq Check: 3720 from: 1 to: 5755
pFN21AB8963 5755 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2689 3818 5202 5470 1632
Size: 1057 1129 1384 268 1917
Fragments arranged by size:
1917 1384 1129 1057 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 2117 1166
Size: 951 4804
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2042 1184
Size: 858 4897
AlwNI CAG_nnn'CTG
Cuts at: 3498 5070 3498
Size: 1572 4183
ApaLI G'TGCA_C
Cuts at: 2533 3812 4968 2533
Size: 1279 1156 3320
Fragments arranged by size:
3320 1279 1156
ApoI r'AATT_y
Cuts at: 1493 2710 2905 3064 3290 3442 5551 1493
Size: 1217 195 159 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 226 195 159 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2518 3080 1870
Size: 73 575 562 4545
Fragments arranged by size:
4545 575 562 73
AvrII C'CTAG_G
Cuts at: 2813 2813
Size: 5755
BamHI G'GATC_C
Cuts at: 2637 2817 3085 3452 2637
Size: 180 268 367 4940
Fragments arranged by size:
4940 367 268 180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2809 3076 4531 618
Size: 325 217 627 1022 267 1455 1842
Fragments arranged by size:
1842 1455 1022 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2761 3074 1391
Size: 1370 313 4072
Fragments arranged by size:
4072 1370 313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3946 3980 1903
Size: 34 50 34 1925 34 3678
Fragments arranged by size:
3678 1925 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 2604 1548
Size: 1056 4699
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2077 4366 136
Size: 107 122 71 631 605 405 2289 1525
Fragments arranged by size:
2289 1525 631 605 405 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 2209 3172 2209
Size: 963 4792
Bme1580I G_kGCm'C
Cuts at: 1790 2537 3816 4972 1790
Size: 747 1279 1156 2573
Fragments arranged by size:
2573 1279 1156 747
BmgBI CAC'GTC
Cuts at: 1318 2983 1318
Size: 1665 4090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 3486 4444
Size: 416 53 83 399 24 1122 947 958
Cuts at: 4444 442
Size: 1753
Fragments arranged by size:
1753 1122 958 947 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2633 4415 1239
Size: 1253 141 1782 2579
Fragments arranged by size:
2579 1782 1253 141
Bpu10I CC'TnA_GC
Cuts at: 2177 2330 2177
Size: 153 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 3880 4745 5043 2387
Size: 1493 865 298 3099
Fragments arranged by size:
3099 1493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2446 3546 493
Size: 711 1242 1100 2702
Fragments arranged by size:
2702 1242 1100 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 3562 3944 4579
Size: 53 83 186 936 551 1478 382 635
Cuts at: 4579 275
Size: 1451
Fragments arranged by size:
1478 1451 936 635 551 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 2751 1186
Size: 1255 310 4190
Fragments arranged by size:
4190 1255 310
BseYI C'CCAG_C
Cuts at: 2040 2754 4958 5454 2040
Size: 714 2204 496 2341
Fragments arranged by size:
2341 2204 714 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 2517 1163
Size: 573 546 235 4401
Fragments arranged by size:
4401 573 546 235
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2478 3205 3966 4994 1540
Size: 275 186 477 727 761 1028 2301
Fragments arranged by size:
2301 1028 761 727 477 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2451 2537 3816 3901 4972 2052
Size: 399 86 1279 85 1071 2835
Fragments arranged by size:
2835 1279 1071 399 86 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
BsmBI CGTCTCn'nnnn_
Cuts at: 3015 3015
Size: 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2451 2537 2761 3074 3816 3901
Size: 399 262 399 86 224 313 742 85
Cuts at: 3901 4972 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 399 313 262 224
86 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 844
Size: 2266 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2360 4399 4586 1700
Size: 110 550 2039 187 2869
Fragments arranged by size:
2869 2039 550 187 110
BsrGI T'GTAC_A
Cuts at: 96 2418 2945 96
Size: 2322 527 2906
Fragments arranged by size:
2906 2322 527
BssHII G'CGCG_C
Cuts at: 1925 2022 2064 1925
Size: 97 42 5616
Fragments arranged by size:
5616 97 42
BssSI C'ACGA_G
Cuts at: 2074 2530 3815 4827 2074
Size: 456 1285 1012 3002
Fragments arranged by size:
3002 1285 1012 456
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2312 2575 2728 2122
Size: 190 263 153 5149
Fragments arranged by size:
5149 263 190 153
BstYI r'GATC_y
Cuts at: 1972 2523 2637 2817 3085 3452 3837 3854 5306
Size: 551 114 180 268 367 385 17 1452
Cuts at: 5306 5392 5404 5750 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 551 385 367 346 268 180
114 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2053 2574 2784 513
Size: 552 988 521 210 3484
Fragments arranged by size:
3484 988 552 521 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2350 2554 487
Size: 683 56 1124 204 3688
Fragments arranged by size:
3688 1124 683 204 56
BtsI GCAGTG_nn'
Cuts at: 2299 3271 4065 4085 2299
Size: 972 794 20 3969
Fragments arranged by size:
3969 972 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2475 2781
Size: 54 1425 50 275 18 402 243 306
Cuts at: 2781 2838 2985 3202 4091 5720 8
Size: 57 147 217 889 1629 43
Fragments arranged by size:
1629 1425 889 402 306 275 243 217
147 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2475 3202 1537
Size: 275 663 727 4090
Fragments arranged by size:
4090 727 663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3685 1353
Size: 2332 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2077 2548 4342 4716 4862 387
Size: 1064 138 488 471 1794 374 146 1280
Fragments arranged by size:
1794 1280 1064 488 471 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2492 2633 2689 3818 4415 5202 5470
Size: 393 860 141 56 1129 597 787 268
Cuts at: 5470 1239
Size: 1524
Fragments arranged by size:
1524 1129 860 787 597 393 268 141
56
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4396 4428 4470 4502 1373
Size: 32 135 32 2824 32 42 32 2626
Fragments arranged by size:
2824 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2840 10
Size: 54 1425 343 402 606 2925
Fragments arranged by size:
2925 1425 606 402 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 2120 2198 2573 2726 3713 4072
Size: 648 951 3 78 375 153 987 359
Cuts at: 4072 4231 518
Size: 159 2042
Fragments arranged by size:
2042 987 951 648 375 359 159 153
78 3
MspA1I CmG'CkG
Cuts at: 1606 2055 2868 3171 3848 4996 5241 1606
Size: 449 813 303 677 1148 245 2120
Fragments arranged by size:
2120 1148 813 677 449 303 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5203
NdeI CA'TA_TG
Cuts at: 387 2456 387
Size: 2069 3686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 3113 4658 1132
Size: 97 1884 1545 2229
Fragments arranged by size:
2229 1884 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2754 1108
Size: 1646 4109
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 3107 830
Size: 2277 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2868 2868
Size: 5755
SacII CC_GC'GG
Cuts at: 2056 2056
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SexAI A'CCwGG_T
Cuts at: 2223 2223
Size: 5755
SfcI C'TryA_G
Cuts at: 826 1045 3103 4238 4919 5110 826
Size: 219 2058 1135 681 191 1471
Fragments arranged by size:
2058 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 3859 4760 5022 820
Size: 197 926 459 1457 901 262 1553
Fragments arranged by size:
1553 1457 926 901 459 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2813 3194 513
Size: 552 1748 381 3074
Fragments arranged by size:
3074 1748 552 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 2945 4001
Size: 274 80 33 51 1443 441 527 1056
Cuts at: 4001 5713 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1056 527 441 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 2848 2575
Size: 273 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI
SrfI SwaI