Restriction map for
pFN21AB8962
(Circular) (Six-base) MAPSORT of: pFN21AB8962.seq Check: 764 from: 1 to: 5731
pFN21AB8962 5731 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 2431 3052 2431
Size: 621 5110
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3794 5178 5446 1632
Size: 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 268
AfeI AGC'GCT
Cuts at: 2075 2075
Size: 5731
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2117 1184
Size: 933 4798
AlwNI CAG_nnn'CTG
Cuts at: 2662 2845 3474 5046 2662
Size: 183 629 1572 3347
Fragments arranged by size:
3347 1572 629 183
ApaI G_GGCC'C
Cuts at: 2672 2672
Size: 5731
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 2861 3040 3266 3418 5527 1493
Size: 1368 179 226 152 2109 1697
Fragments arranged by size:
2109 1697 1368 226 179 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2375 2461 3056 1870
Size: 73 432 86 595 4545
Fragments arranged by size:
4545 595 432 86 73
BamHI G'GATC_C
Cuts at: 2997 3061 3428 2997
Size: 64 367 5300
Fragments arranged by size:
5300 367 64
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 2820 3052 4507 618
Size: 325 217 627 644 389 232 1455 1842
Fragments arranged by size:
1842 1455 644 627 389 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2049 2151 2672 2684 3050 1391
Size: 658 102 521 12 366 4072
Fragments arranged by size:
4072 658 521 366 102 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4760
BbvCI CC'TCA_GC
Cuts at: 2574 2574
Size: 5731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2667 4342 136
Size: 107 122 71 631 605 995 1675 1525
Fragments arranged by size:
1675 1525 995 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 2442 2672 2825 3792 4948 1790
Size: 652 230 153 967 1156 2573
Fragments arranged by size:
2573 1156 967 652 230 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2707 2921 4391 1239
Size: 1468 214 1470 2579
Fragments arranged by size:
2579 1470 1468 214
Bpu10I CC'TnA_GC
Cuts at: 2574 2574
Size: 5731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2957 3856 4721 5019 2957
Size: 899 865 298 3669
Fragments arranged by size:
3669 899 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 4394 882
Size: 617 1098 1797 2219
Fragments arranged by size:
2219 1797 1098 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 493
Size: 711 2318 2702
Fragments arranged by size:
2702 2318 711
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2486 3538 3920 4555
Size: 53 83 186 936 953 1052 382 635
Cuts at: 4555 275
Size: 1451
Fragments arranged by size:
1451 1052 953 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2082 2339 2771 1186
Size: 896 257 432 4146
Fragments arranged by size:
4146 896 432 257
BseYI C'CCAG_C
Cuts at: 2189 2441 2578 2630 4934 5430 2189
Size: 252 137 52 2304 496 2490
Fragments arranged by size:
2490 2304 496 252 137 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 3792 3877 4948 2427
Size: 1365 85 1071 3210
Fragments arranged by size:
3210 1365 1071 85
BsiWI C'GTAC_G
Cuts at: 2037 2037
Size: 5731
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2151 2427 2442 2672 2684 2825
Size: 399 259 102 276 15 230 12 141
Cuts at: 2825 3050 3792 3877 4948 1391
Size: 225 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 259 230 225
141 102 85 15 12
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3086 844
Size: 2242 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2805 3618 1394
Size: 429 982 813 3507
Fragments arranged by size:
3507 982 813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2026 4226 4400 65
Size: 1183 778 2200 174 1396
Fragments arranged by size:
2200 1396 1183 778 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 2343 96
Size: 2247 3484
BssHII G'CGCG_C
Cuts at: 1925 2551 1925
Size: 626 5105
BssSI C'ACGA_G
Cuts at: 2426 3791 4803 2426
Size: 1365 1012 3354
Fragments arranged by size:
3354 1365 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 2514 4535 2514
Size: 2021 3710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstXI CCAn_nnnn'nTGG
Cuts at: 2498 2498
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 2997 3061 3428 3813 3830 5282 5368 5380
Size: 1025 64 367 385 17 1452 86 12
Cuts at: 5380 5726 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1025 385 367 346 86 64
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 3247 4041 4061 3247
Size: 794 20 4917
Fragments arranged by size:
4917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DraIII CAC_nnn'GTG
Cuts at: 2315 2315
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2611 2936 4738 809
Size: 507 1295 325 1802 1802
Fragments arranged by size:
1802 1802 1295 507 325
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696
Size: 54 1425 50 275 18 1348 889 1629
Cuts at: 5696 8
Size: 43
Fragments arranged by size:
1629 1425 1348 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2810 3661 1353
Size: 1457 851 3423
Fragments arranged by size:
3423 1457 851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 4318 4692 4838 387
Size: 1064 138 490 2239 374 146 1280
Fragments arranged by size:
2239 1280 1064 490 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2707 2921 3794 4391 5178 5446 1239
Size: 393 1075 214 873 597 787 268 1524
Fragments arranged by size:
1524 1075 873 787 597 393 268 214
EcoO109I rG'GnC_Cy
Cuts at: 2669 2669
Size: 5731
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 2077 2896 4878 1678
Size: 399 819 1982 2531
Fragments arranged by size:
2531 1982 819 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 4372 4404 4446
Size: 32 135 32 427 32 2341 32 42
Cuts at: 4446 4478 1373
Size: 32 2626
Fragments arranged by size:
2626 2341 427 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 2435 3056 2435
Size: 621 5110
MfeI C'AATT_G
Cuts at: 2122 3330 2122
Size: 1208 4523
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2640 4845 5029 1305
Size: 1335 2205 184 2007
Fragments arranged by size:
2205 2007 1335 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3909
Fragments arranged by size:
3909 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 3689 4048 4207 518
Size: 648 933 1590 359 159 2042
Fragments arranged by size:
2042 1590 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2230 2290 2533 2578 3147 3824 4972 5217
Size: 624 60 243 45 569 677 1148 245
Cuts at: 5217 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 624 569 245 243 60
45
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5179
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2013 2098 2344 3089 4634 1132
Size: 97 784 85 246 745 1545 2229
Fragments arranged by size:
2229 1545 784 745 246 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2845 1108
Size: 1737 3994
PfoI T'CCnGG_A
Cuts at: 3000 3000
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PspOMI G'GGCC_C
Cuts at: 2668 2668
Size: 5731
PstI C_TGCA'G
Cuts at: 830 3083 830
Size: 2253 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2230 2578 2230
Size: 348 5383
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2077 3079 4214 4895 5086 826
Size: 219 1032 1002 1135 681 191 1471
Fragments arranged by size:
1471 1135 1032 1002 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 3058 1872
Size: 1186 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2972 3835 4736 4998 820
Size: 197 926 1029 863 901 262 1553
Fragments arranged by size:
1553 1029 926 901 863 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 2049 2416 3170 513
Size: 552 984 367 754 3074
Fragments arranged by size:
3074 984 754 552 367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3758 3775 3928 4113 4558 325
Size: 1255 2178 17 153 185 445 1498
Fragments arranged by size:
2178 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 3977 5689
Size: 274 80 33 51 1443 366 1634 1712
Cuts at: 5689 96
Size: 138
Fragments arranged by size:
1712 1634 1443 366 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 3056 1870
Size: 1186 4545
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI