Restriction map for
pFN21AB8959
(Circular) (Six-base) MAPSORT of: pFN21AB8959.seq Check: 6039 from: 1 to: 5719
pFN21AB8959 5719 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2475 2475
Size: 5719
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 5166 5434 1632
Size: 2150 1384 268 1917
Fragments arranged by size:
2150 1917 1384 268
AfeI AGC'GCT
Cuts at: 2582 2582
Size: 5719
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 2545 4448 2545
Size: 1903 3816
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaLI G'TGCA_C
Cuts at: 2336 3776 4932 2336
Size: 1440 1156 3123
Fragments arranged by size:
3123 1440 1156
ApoI r'AATT_y
Cuts at: 1493 2063 3028 3254 3406 5515 1493
Size: 570 965 226 152 2109 1697
Fragments arranged by size:
2109 1697 965 570 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2870 3044 1870
Size: 73 927 174 4545
Fragments arranged by size:
4545 927 174 73
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2453 2851 3040 4495 618
Size: 325 217 627 666 398 189 1455 1842
Fragments arranged by size:
1842 1455 666 627 398 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2669 2808 3038 1391
Size: 1278 139 230 4072
Fragments arranged by size:
4072 1278 230 139
BbeI G_GCGC'C
Cuts at: 2457 2855 2457
Size: 398 5321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4748
BbvCI CC'TCA_GC
Cuts at: 2534 2534
Size: 5719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2827 2861 3910 3944 1903
Size: 34 890 34 1049 34 3678
Fragments arranged by size:
3678 1049 890 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2352 4330
Size: 107 122 71 631 605 337 343 1978
Cuts at: 4330 136
Size: 1525
Fragments arranged by size:
1978 1525 631 605 343 337 122 107
71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 2672 3136 2672
Size: 464 5255
Bme1580I G_kGCm'C
Cuts at: 1790 2265 2292 2340 3780 4936 1790
Size: 475 27 48 1440 1156 2573
Fragments arranged by size:
2573 1440 1156 475 48 27
BmgBI CAC'GTC
Cuts at: 1318 2271 2542 2626 2841 2897 1318
Size: 953 271 84 215 56 4140
Fragments arranged by size:
4140 953 271 215 84 56
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3450 4408 442
Size: 416 53 83 399 24 2033 958 1753
Fragments arranged by size:
2033 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2126 2389 2126
Size: 263 5456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 2384 2732 4379 1239
Size: 814 331 348 1647 2579
Fragments arranged by size:
2579 1647 814 348 331
Bpu10I CC'TnA_GC
Cuts at: 2534 2534
Size: 5719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2720 3844 4709 5007 2633
Size: 87 1124 865 298 3345
Fragments arranged by size:
3345 1124 865 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 2454 2852 3526
Size: 53 83 186 936 702 219 398 674
Cuts at: 3526 3908 4543 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 702 674 635 398 382 219
186 83 53
BsaWI w'CCGG_w
Cuts at: 2407 4152 4550 4824 4971 5382 2407
Size: 1745 398 274 147 411 2744
Fragments arranged by size:
2744 1745 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2099 1178
Size: 30 165 30 666 30 4798
Fragments arranged by size:
4798 666 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2777 1186
Size: 1591 4128
BseYI C'CCAG_C
Cuts at: 2113 2302 2605 4922 5418 2113
Size: 189 303 2317 496 2414
Fragments arranged by size:
2414 2317 496 303 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 2251 2453 1163
Size: 573 324 191 202 4429
Fragments arranged by size:
4429 573 324 202 191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2370 2748 2808 3780 3865 4936 2340
Size: 30 378 60 972 85 1071 3123
Fragments arranged by size:
3123 1071 972 378 85 60 30
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
BsmBI CGTCTCn'nnnn_
Cuts at: 2206 2206
Size: 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2292 2340 2370 2669 2748 2808
Size: 399 475 27 48 30 299 79 60
Cuts at: 2808 3038 3780 3865 4936 1391
Size: 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 399 299 230 85
79 60 48 30 27
BspEI T'CCGG_A
Cuts at: 2407 5382 2407
Size: 2975 2744
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2367 2475 2682 3074 844
Size: 1523 108 207 392 3489
Fragments arranged by size:
3489 1523 392 207 108
BsrBI CCG'CTC
Cuts at: 1394 1823 2394 3606 1394
Size: 429 571 1212 3507
Fragments arranged by size:
3507 1212 571 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4214 4388 65
Size: 1183 2966 174 1396
Fragments arranged by size:
2966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2740 4363 4550 1700
Size: 110 930 1623 187 2869
Fragments arranged by size:
2869 1623 930 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2047 2688 794
Size: 1253 641 3825
Fragments arranged by size:
3825 1253 641
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 2611 1184
Size: 1427 4292
BstYI r'GATC_y
Cuts at: 1972 2433 2652 3049 3416 3801 3818 5270 5356
Size: 461 219 397 367 385 17 1452 86
Cuts at: 5356 5368 5714 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 461 397 385 367 346 219
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2206 2457 2478 2502 513
Size: 552 1141 251 21 24 3730
Fragments arranged by size:
3730 1141 552 251 24 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2830 2908 487
Size: 683 56 1604 78 3298
Fragments arranged by size:
3298 1604 683 78 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DraIII CAC_nnn'GTG
Cuts at: 2047 2335 2419 2503 2047
Size: 288 84 84 5263
Fragments arranged by size:
5263 288 84 84
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2919 3166
Size: 54 1425 50 275 18 180 909 247
Cuts at: 3166 4055 5684 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 909 889 275 247 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2813 3649 1353
Size: 1460 836 3423
Fragments arranged by size:
3423 1460 836
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2636 4306 4680 4826 387
Size: 1064 138 1047 1670 374 146 1280
Fragments arranged by size:
1670 1280 1064 1047 374 146 138
EcoICRI GAG'CTC
Cuts at: 2806 2806
Size: 5719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2384 2732 3782 4379 5166 5434
Size: 393 421 331 348 1050 597 787 268
Cuts at: 5434 1239
Size: 1524
Fragments arranged by size:
1524 1050 787 597 421 393 348 331
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 2471
Size: 5719
EcoO109I rG'GnC_Cy
Cuts at: 2344 2344
Size: 5719
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
EcoRV GAT'ATC
Cuts at: 2197 2197
Size: 5719
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2406 2457 2520 2584 2855 4866 1678
Size: 728 51 63 64 271 2011 2531
Fragments arranged by size:
2531 2011 728 271 64 63 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2531 2563 4360
Size: 32 135 32 614 32 313 32 1797
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1797 614 313 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2146 748
Size: 1398 4321
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KasI G'GCGC_C
Cuts at: 2453 2851 2453
Size: 398 5321
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2739 4833 5017 1305
Size: 1434 2094 184 2007
Fragments arranged by size:
2094 2007 1434 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 10
Size: 54 1425 343 180 3717
Fragments arranged by size:
3717 1425 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2483 3677 4036 4195 518
Size: 648 1317 1194 359 159 2042
Fragments arranged by size:
2042 1317 1194 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 2306 2593 3135 3812 4960 5205 1606
Size: 519 181 287 542 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 542 519 287 245 181
NarI GG'CG_CC
Cuts at: 2454 2852 2454
Size: 398 5321
NcoI C'CATG_G
Cuts at: 513 1065 2457 2478 513
Size: 552 1392 21 3754
Fragments arranged by size:
3754 1392 552 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2041 2532 3077 4622 1132
Size: 97 812 491 545 1545 2229
Fragments arranged by size:
2229 1545 812 545 491 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 1108
Size: 1371 4348
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspXI vC'TCGA_Gb
Cuts at: 2870 2870
Size: 5719
PstI C_TGCA'G
Cuts at: 830 2130 2693 3071 830
Size: 1300 563 378 3478
Fragments arranged by size:
3478 1300 563 378
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2125 2306 2125
Size: 181 5538
SacI G_AGCT'C
Cuts at: 2808 2808
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SapI GCTCTTCn'nnn_
Cuts at: 2813 2813
Size: 5719
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 2126 2689 3067 4202 4883 5074 826
Size: 219 1081 563 378 1135 681 191 1471
Fragments arranged by size:
1471 1135 1081 681 563 378 219 191
SfoI GGC'GCC
Cuts at: 2455 2853 2455
Size: 398 5321
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2648 2735 2870 3823 4724 4986
Size: 197 926 705 87 135 953 901 262
Cuts at: 4986 820
Size: 1553
Fragments arranged by size:
1553 953 926 901 705 262 197 135
87
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 2041 2532 3077 1132
Size: 909 491 545 3774
Fragments arranged by size:
3774 909 545 491
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 2753 2876 1517
Size: 1236 123 4360
Fragments arranged by size:
4360 1236 123
StyI C'CwwG_G
Cuts at: 513 1065 2457 2478 3158 513
Size: 552 1392 21 680 3074
Fragments arranged by size:
3074 1392 680 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2316 3746 3763 3916 4101 4546 325
Size: 1255 736 1430 17 153 185 445 1498
Fragments arranged by size:
1498 1430 1255 736 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3965 5677 96
Size: 274 80 33 51 1443 1988 1712 138
Fragments arranged by size:
1988 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 5679 5711 2069
Size: 32 3578 32 2077
Fragments arranged by size:
3578 2077 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 2870 1943
Size: 927 4792
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI XcmI