Restriction map for
pFN21AB8958
(Circular) (Six-base) MAPSORT of: pFN21AB8958.seq Check: 2792 from: 1 to: 5710
pFN21AB8958 5710 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 2370 3031 2370
Size: 661 5049
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2299 3773 5157 5425 1632
Size: 667 1474 1384 268 1917
Fragments arranged by size:
1917 1474 1384 667 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 2337 4609 5586 1203
Size: 1134 2272 977 1327
Fragments arranged by size:
2272 1327 1134 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2195 1184
Size: 1011 4699
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 5678
AlwNI CAG_nnn'CTG
Cuts at: 2515 3453 5025 2515
Size: 938 1572 3200
Fragments arranged by size:
3200 1572 938
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2079 2971 3035 1870
Size: 73 119 17 892 64 4545
Fragments arranged by size:
4545 892 119 73 64 17
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2370 3031 4486 618
Size: 325 217 627 583 661 1455 1842
Fragments arranged by size:
1842 1455 661 627 583 325 217
BanII G_rGCy'C
Cuts at: 1391 2471 3029 1391
Size: 1080 558 4072
Fragments arranged by size:
4072 1080 558
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 928
Size: 971 1071 3668
Fragments arranged by size:
3668 1071 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2826 5705 2826
Size: 2879 2831
BlpI GC'TnA_GC
Cuts at: 2074 3127 2074
Size: 1053 4657
Bme1580I G_kGCm'C
Cuts at: 1790 3771 4927 1790
Size: 1981 1156 2573
Fragments arranged by size:
2573 1981 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 3441 4399
Size: 416 53 83 399 24 954 1070 958
Cuts at: 4399 442
Size: 1753
Fragments arranged by size:
1753 1070 958 954 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2589 2756 4370 1239
Size: 1350 167 1614 2579
Fragments arranged by size:
2579 1614 1350 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2266 3835 4700 4998 2266
Size: 1569 865 298 2978
Fragments arranged by size:
2978 1569 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2807 2882 4373 882
Size: 617 1308 75 1491 2219
Fragments arranged by size:
2219 1491 1308 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 2529 3501 493
Size: 711 1325 972 2702
Fragments arranged by size:
2702 1325 972 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2587 2617 2729 2759 1178
Size: 30 165 30 1184 30 112 30 4129
Fragments arranged by size:
4129 1184 165 112 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5710
BseYI C'CCAG_C
Cuts at: 2229 2453 4913 5409 2229
Size: 224 2460 496 2530
Fragments arranged by size:
2530 2460 496 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 1163
Size: 573 465 4672
Fragments arranged by size:
4672 573 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2158 3160 3921 4949 1540
Size: 275 186 157 1002 761 1028 2301
Fragments arranged by size:
2301 1028 1002 761 275 186 157
BsiHKAI G_wGCw'C
Cuts at: 2508 3771 3856 4927 2508
Size: 1263 85 1071 3291
Fragments arranged by size:
3291 1263 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2471 2508 3029 3771 3856 4927 1391
Size: 399 681 37 521 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 681 521 399 85 37
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2078 3065 844
Size: 1234 987 3489
Fragments arranged by size:
3489 1234 987
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2233 4354 4541 1700
Size: 110 423 2121 187 2869
Fragments arranged by size:
2869 2121 423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 2770 1925
Size: 845 4865
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2495 2495
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2826 3040 3407 3792 3809 5261 5347 5359
Size: 854 214 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 854 385 367 346 214 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 3805
Fragments arranged by size:
3805 939 552 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 3226 4020 4040 2059
Size: 1167 794 20 3729
Fragments arranged by size:
3729 1167 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2267 2302 529
Size: 35 1703 35 3937
Fragments arranged by size:
3937 1703 35 35
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2895 3157 4046
Size: 54 1425 50 275 18 1065 262 889
Cuts at: 4046 5675 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1065 889 275 262 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2956 3640 1353
Size: 1603 684 3423
Fragments arranged by size:
3423 1603 684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 2589 2756 3773 4370 5157 5425
Size: 393 667 290 167 1017 597 787 268
Cuts at: 5425 1239
Size: 1524
Fragments arranged by size:
1524 1017 787 667 597 393 290 268
167
EcoO109I rG'GnC_Cy
Cuts at: 2067 2067
Size: 5710
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 2702 4216 2702
Size: 1514 4196
FspAI rTGC'GCAy
Cuts at: 2702 2702
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2136 4857 1678
Size: 458 2721 2531
Fragments arranged by size:
2721 2531 458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2587 2619 4351 4383 4425
Size: 32 135 32 1015 32 1732 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 1732 1015 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2687 3054 3300 669
Size: 988 281 749 367 246 3079
Fragments arranged by size:
3079 988 749 367 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 2374 3035 2374
Size: 661 5049
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 2337 5586 2337
Size: 3249 2461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2644 4824 5008 1305
Size: 1339 2180 184 2007
Fragments arranged by size:
2180 2007 1339 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2493 3668 4027 4186 518
Size: 648 1327 1175 359 159 2042
Fragments arranged by size:
2042 1327 1175 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2390 3126 3803 4951 5196 1606
Size: 784 736 677 1148 245 2120
Fragments arranged by size:
2120 1148 784 736 677 245
NaeI GCC'GGC
Cuts at: 2235 2235
Size: 5710
NcoI C'CATG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 3805
Fragments arranged by size:
3805 939 552 414
NdeI CA'TA_TG
Cuts at: 387 2494 387
Size: 2107 3603
NgoMIV G'CCGG_C
Cuts at: 2233 2233
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2300 2515 1108
Size: 1192 215 4303
Fragments arranged by size:
4303 1192 215
PfoI T'CCnGG_A
Cuts at: 2031 2252 2031
Size: 221 5489
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2761 3779 3811 5292 5324 2729
Size: 32 1018 32 1481 32 3115
Fragments arranged by size:
3115 1481 1018 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2067 2067
Size: 5710
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2805 3062 830
Size: 1975 257 3478
Fragments arranged by size:
3478 1975 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SapI GCTCTTCn'nnn_
Cuts at: 2956 2956
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2801 3058 4193 4874 5065 826
Size: 219 1756 257 1135 681 191 1471
Fragments arranged by size:
1756 1471 1135 681 257 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 2973 3037 1872
Size: 1101 64 4545
Fragments arranged by size:
4545 1101 64
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2281 3814 4715 4977 820
Size: 197 926 338 1533 901 262 1553
Fragments arranged by size:
1553 1533 926 901 338 262 197
SnaBI TAC'GTA
Cuts at: 493 2529 3501 493
Size: 2036 972 2702
Fragments arranged by size:
2702 2036 972
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2418 3149 513
Size: 552 939 414 731 3074
Fragments arranged by size:
3074 939 731 552 414
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3737 3754 3907 4092 4537 325
Size: 1255 2157 17 153 185 445 1498
Fragments arranged by size:
2157 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2285 2450 2285
Size: 165 5545
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 2971 3035 1870
Size: 1101 64 4545
Fragments arranged by size:
4545 1101 64
XmnI GAAnn'nnTTC
Cuts at: 2027 2744 3839 2027
Size: 717 1095 3898
Fragments arranged by size:
3898 1095 717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI