Restriction map for
pFN21AB8957
(Circular) (Six-base) MAPSORT of: pFN21AB8957.seq Check: 5857 from: 1 to: 5704
pFN21AB8957 5704 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2655 3047 1937
Size: 718 392 4594
Fragments arranged by size:
4594 718 392
Acc65I G'GTAC_C
Cuts at: 2805 3025 2805
Size: 220 5484
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2670 3767 5151 5419 1632
Size: 1038 1097 1384 268 1917
Fragments arranged by size:
1917 1384 1097 1038 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 2833 2845 3447 5019 2833
Size: 12 602 1572 3518
Fragments arranged by size:
3518 1572 602 12
ApaLI G'TGCA_C
Cuts at: 2936 3761 4917 2936
Size: 825 1156 3723
Fragments arranged by size:
3723 1156 825
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2113 2234 2374 2382 3029 1870
Size: 73 146 24 121 140 8 647 4545
Fragments arranged by size:
4545 647 146 140 121 73 24 8
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2133 2170 2501 2612 2805
Size: 325 217 627 346 37 331 111 193
Cuts at: 2805 2823 3025 4480 618
Size: 18 202 1455 1842
Fragments arranged by size:
1842 1455 627 346 331 325 217 202
193 111 37 18
BanII G_rGCy'C
Cuts at: 1391 2146 2375 2381 2391 2588 3023 1391
Size: 755 229 6 10 197 435 4072
Fragments arranged by size:
4072 755 435 229 197 10 6
BbeI G_GCGC'C
Cuts at: 2137 2174 2505 2616 2827 2137
Size: 37 331 111 211 5014
Fragments arranged by size:
5014 331 211 111 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2082 4315 136
Size: 107 122 71 631 605 410 2233 1525
Fragments arranged by size:
2233 1525 631 605 410 122 107 71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 2846 3121 2846
Size: 275 5429
Bme1580I G_kGCm'C
Cuts at: 1790 2940 3765 4921 1790
Size: 1150 825 1156 2573
Fragments arranged by size:
2573 1156 1150 825
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3435 4393 442
Size: 416 53 83 399 24 2018 958 1753
Fragments arranged by size:
2018 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4364 1239
Size: 3125 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 2765 3829 4694 4992 2531
Size: 234 1064 865 298 3243
Fragments arranged by size:
3243 1064 865 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2703 4367 882
Size: 617 1204 1664 2219
Fragments arranged by size:
2219 1664 1204 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2134 2171 2502 2613
Size: 53 83 186 936 601 37 331 111
Cuts at: 2613 2824 3511 3893 4528 275
Size: 211 687 382 635 1451
Fragments arranged by size:
1451 936 687 635 601 382 331 211
186 111 83 53 37
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2951 2975 2981 3005 1178
Size: 30 165 30 1548 24 6 24 3877
Fragments arranged by size:
3877 1548 165 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2298 1186
Size: 838 274 4592
Fragments arranged by size:
4592 838 274
BseYI C'CCAG_C
Cuts at: 2434 4907 5403 2434
Size: 2473 496 2735
Fragments arranged by size:
2735 2473 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2477 1163
Size: 573 741 4390
Fragments arranged by size:
4390 741 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2245 2744 3154 3915 4943 1540
Size: 275 186 244 499 410 761 1028 2301
Fragments arranged by size:
2301 1028 761 499 410 275 244 186
BsiHKAI G_wGCw'C
Cuts at: 2588 2940 3765 3850 4921 2588
Size: 352 825 85 1071 3371
Fragments arranged by size:
3371 1071 825 352 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2375 2381 2391 2588 2940 3023
Size: 399 356 229 6 10 197 352 83
Cuts at: 3023 3765 3850 4921 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 356 352 229 197
85 83 10 6
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2273 3059 844
Size: 1429 786 3489
Fragments arranged by size:
3489 1429 786
BsrBI CCG'CTC
Cuts at: 1394 1823 2325 2431 3591 1394
Size: 429 502 106 1160 3507
Fragments arranged by size:
3507 1160 502 429 106
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 2244 2303 2441 2609 2644 2676
Size: 110 326 108 59 138 168 35 32
Cuts at: 2676 4348 4535 1700
Size: 1672 187 2869
Fragments arranged by size:
2869 1672 326 187 168 138 110 108
59 35 32
BsrGI T'GTAC_A
Cuts at: 96 2958 96
Size: 2862 2842
BssHII G'CGCG_C
Cuts at: 1925 2625 2890 1925
Size: 700 265 4739
Fragments arranged by size:
4739 700 265
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2635 794
Size: 1841 3863
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 3034 3401 3786 3803 5255 5341 5353 5699
Size: 1062 367 385 17 1452 86 12 346
Cuts at: 5699 1972
Size: 1977
Fragments arranged by size:
1977 1452 1062 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 2554 2910 513
Size: 552 939 153 397 356 3307
Fragments arranged by size:
3307 939 552 397 356 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 3220 4014 4034 3220
Size: 794 20 4890
Fragments arranged by size:
4890 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2242 2642 2741
Size: 54 1425 50 275 18 412 400 99
Cuts at: 2741 2931 3151 4040 5669 8
Size: 190 220 889 1629 43
Fragments arranged by size:
1629 1425 889 412 400 275 220 190
99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2242 2741 3151 1537
Size: 275 430 499 410 4090
Fragments arranged by size:
4090 499 430 410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3634 1353
Size: 2281 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2313 4291 4665 4811 387
Size: 1064 138 724 1978 374 146 1280
Fragments arranged by size:
1978 1280 1064 724 374 146 138
EcoICRI GAG'CTC
Cuts at: 2586 2586
Size: 5704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2670 3767 4364 5151 5419 1239
Size: 393 1038 1097 597 787 268 1524
Fragments arranged by size:
1524 1097 1038 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2685 2837 2685
Size: 152 5552
EcoO109I rG'GnC_Cy
Cuts at: 2262 2690 2262
Size: 428 5276
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 2137 2174 2505 2616 2827 4851 1678
Size: 459 37 331 111 211 2024 2531
Fragments arranged by size:
2531 2024 459 331 211 111 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2975 3007 4345 4377 4419
Size: 32 135 32 1403 32 1338 32 42
Cuts at: 4419 4451 1373
Size: 32 2626
Fragments arranged by size:
2626 1403 1338 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KasI G'GCGC_C
Cuts at: 2133 2170 2501 2612 2823 2133
Size: 37 331 111 211 5014
Fragments arranged by size:
5014 331 211 111 37
KpnI G_GTAC'C
Cuts at: 2809 3029 2809
Size: 220 5484
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2973 4818 5002 1305
Size: 1668 1845 184 2007
Fragments arranged by size:
2007 1845 1668 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3882
Fragments arranged by size:
3882 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3662 4021 4180 518
Size: 648 2496 359 159 2042
Fragments arranged by size:
2496 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2059 2159 2556 2770 2821 3120 3797
Size: 433 20 100 397 214 51 299 677
Cuts at: 3797 4945 5190 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 433 397 299 245 214
100 51 20
NaeI GCC'GGC
Cuts at: 2138 2246 2305 2443 2611 2646 2138
Size: 108 59 138 168 35 5196
Fragments arranged by size:
5196 168 138 108 59 35
NarI GG'CG_CC
Cuts at: 2134 2171 2502 2613 2824 2134
Size: 37 331 111 211 5014
Fragments arranged by size:
5014 331 211 111 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4213
Fragments arranged by size:
4213 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NgoMIV G'CCGG_C
Cuts at: 2136 2244 2303 2441 2609 2644 2136
Size: 108 59 138 168 35 5196
Fragments arranged by size:
5196 168 138 108 59 35
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 2741 3151 2741
Size: 410 5294
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2640 2833 1108
Size: 1532 193 3979
Fragments arranged by size:
3979 1532 193
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2262 2690 2262
Size: 428 5276
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2169 2775 3056 830
Size: 1339 606 281 3478
Fragments arranged by size:
3478 1339 606 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
PvuII CAG'CTG
Cuts at: 2770 2770
Size: 5704
RsrII CG'GwC_CG
Cuts at: 2674 2674
Size: 5704
SacI G_AGCT'C
Cuts at: 2588 2588
Size: 5704
SacII CC_GC'GG
Cuts at: 2160 2557 2160
Size: 397 5307
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 2169 3056 2169
Size: 887 4817
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 2165 2771 3052 4187 4868 5059 826
Size: 219 1120 606 281 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 681 606 281 219 191
SfoI GGC'GCC
Cuts at: 2135 2172 2503 2614 2825 2135
Size: 37 331 111 211 5014
Fragments arranged by size:
5014 331 211 111 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 2136 1700
Size: 436 5268
SmaI CCC'GGG
Cuts at: 1872 2115 3031 1872
Size: 243 916 4545
Fragments arranged by size:
4545 916 243
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 2780 3808 4709 4971 820
Size: 197 926 603 234 1028 901 262 1553
Fragments arranged by size:
1553 1028 926 901 603 262 234 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SrfI GCCC'GGGC
Cuts at: 2115 2115
Size: 5704
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2004 2979 3143 513
Size: 552 939 975 164 3074
Fragments arranged by size:
3074 975 939 552 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3731 3748 3901 4086 4531 325
Size: 1255 2151 17 153 185 445 1498
Fragments arranged by size:
2151 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2958 3950 5662
Size: 274 80 33 51 1443 981 992 1712
Cuts at: 5662 96
Size: 138
Fragments arranged by size:
1712 1443 992 981 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2254 2286 5664 5696 2254
Size: 32 3378 32 2262
Fragments arranged by size:
3378 2262 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 2113 3029 1870
Size: 243 916 4545
Fragments arranged by size:
4545 916 243
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI SanDI SapI SexAI
SfiI SwaI XcmI