Restriction map for
pFN21AB8951
(Circular) (Six-base) MAPSORT of: pFN21AB8951.seq Check: 3112 from: 1 to: 5683
pFN21AB8951 5683 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2249 2249
Size: 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2547 3026 1937
Size: 610 479 4594
Fragments arranged by size:
4594 610 479
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2714 3746 5130 5398 1632
Size: 1082 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1082 1032 268
AflII C'TTAA_G
Cuts at: 820 1017 2903 820
Size: 197 1886 3600
Fragments arranged by size:
3600 1886 197
AflIII A'CryG_T
Cuts at: 1203 4582 5559 1203
Size: 3379 977 1327
Fragments arranged by size:
3379 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 1184
Size: 885 4798
AlwNI CAG_nnn'CTG
Cuts at: 2122 3426 4998 2122
Size: 1304 1572 2807
Fragments arranged by size:
2807 1572 1304
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 3008 1870
Size: 73 135 930 4545
Fragments arranged by size:
4545 930 135 73
BamHI G'GATC_C
Cuts at: 2400 3013 3380 2400
Size: 613 367 4703
Fragments arranged by size:
4703 613 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 3004 4459 618
Size: 325 217 627 393 824 1455 1842
Fragments arranged by size:
1842 1455 824 627 393 325 217
BanII G_rGCy'C
Cuts at: 1391 2285 2499 3002 1391
Size: 894 214 503 4072
Fragments arranged by size:
4072 894 503 214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2605 928
Size: 971 706 4006
Fragments arranged by size:
4006 971 706
BbvCI CC'TCA_GC
Cuts at: 2738 2738
Size: 5683
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3874 3908 1903
Size: 34 50 34 1853 34 3678
Fragments arranged by size:
3678 1853 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 2502 1548
Size: 954 4729
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2300 4294 136
Size: 107 122 71 631 605 628 1994 1525
Fragments arranged by size:
1994 1525 631 628 605 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2185 3744 4900 1790
Size: 289 106 1559 1156 2573
Fragments arranged by size:
2573 1559 1156 289 106
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 4343 1239
Size: 1388 1716 2579
Fragments arranged by size:
2579 1716 1388
Bpu10I CC'TnA_GC
Cuts at: 2738 2738
Size: 5683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 4346 882
Size: 617 575 2272 2219
Fragments arranged by size:
2272 2219 617 575
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2260 2343 4116 4514 4788 4935 5346 2260
Size: 83 1773 398 274 147 411 2597
Fragments arranged by size:
2597 1773 411 398 274 147 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5458
Fragments arranged by size:
5458 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2576 2647 1186
Size: 1390 71 4222
Fragments arranged by size:
4222 1390 71
BseYI C'CCAG_C
Cuts at: 2801 4886 5382 2801
Size: 2085 496 3102
Fragments arranged by size:
3102 2085 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2922 1163
Size: 573 1186 3924
Fragments arranged by size:
3924 1186 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 2285 3744 3829 4900 2058
Size: 227 1459 85 1071 2841
Fragments arranged by size:
2841 1459 1071 227 85
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2079 2185 2285 2499 3002 3744
Size: 399 268 21 106 100 214 503 742
Cuts at: 3744 3829 4900 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 503 399 268 214 106
100 85 21
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2249 3038 844
Size: 1405 789 3489
Fragments arranged by size:
3489 1405 789
BsrBI CCG'CTC
Cuts at: 1394 1823 2464 3570 1394
Size: 429 641 1106 3507
Fragments arranged by size:
3507 1106 641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2817 4178 4352 65
Size: 1183 1569 1361 174 1396
Fragments arranged by size:
1569 1396 1361 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2390 4327 4514 1700
Size: 110 580 1937 187 2869
Fragments arranged by size:
2869 1937 580 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 2359 1184
Size: 1175 4508
BstXI CCAn_nnnn'nTGG
Cuts at: 2710 2710
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 2334 2400 2956 3013 3380 3765 3782 5234
Size: 362 66 556 57 367 385 17 1452
Cuts at: 5234 5320 5332 5678 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 556 385 367 362 346 86
66 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2175 513
Size: 552 1110 4021
Fragments arranged by size:
4021 1110 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2044 487
Size: 683 56 818 4126
Fragments arranged by size:
4126 818 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2292 2327 529
Size: 35 1728 35 3885
Fragments arranged by size:
3885 1728 35 35
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2382 2847
Size: 54 1425 50 275 18 342 210 465
Cuts at: 2847 3130 4019 5648 8
Size: 283 889 1629 43
Fragments arranged by size:
1629 1425 889 465 342 283 275 210
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2729 2783 2961 3613 1353
Size: 1376 54 178 652 3423
Fragments arranged by size:
3423 1376 652 178 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2283 2283
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2627 2714 3746 4343 5130 5398 1239
Size: 393 995 87 1032 597 787 268 1524
Fragments arranged by size:
1524 1032 995 787 597 393 268 87
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 2123 4830 1678
Size: 445 2707 2531
Fragments arranged by size:
2707 2531 445
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4324 4356 4398 4430 1373
Size: 32 135 32 2752 32 42 32 2626
Fragments arranged by size:
2752 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 2304 3282 2304
Size: 978 4705
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 10
Size: 54 1425 343 552 3309
Fragments arranged by size:
3309 1425 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 2531 3641 4000 4159 518
Size: 648 885 480 1110 359 159 2042
Fragments arranged by size:
2042 1110 885 648 480 359 159
MspA1I CmG'CkG
Cuts at: 1606 2742 2928 3099 3776 4924 5169 1606
Size: 1136 186 171 677 1148 245 2120
Fragments arranged by size:
2120 1148 1136 677 245 186 171
NaeI GCC'GGC
Cuts at: 2392 2392
Size: 5683
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5131
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NgoMIV G'CCGG_C
Cuts at: 2390 2390
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 3041 4586 1132
Size: 97 1812 1545 2229
Fragments arranged by size:
2229 1812 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 3035 830
Size: 2205 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2742 2742
Size: 5683
SacI G_AGCT'C
Cuts at: 2285 2285
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 2678 3931 5643 2678
Size: 1253 1712 2718
Fragments arranged by size:
2718 1712 1253
SfcI C'TryA_G
Cuts at: 826 1045 3031 4166 4847 5038 826
Size: 219 1986 1135 681 191 1471
Fragments arranged by size:
1986 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 2080 3010 1872
Size: 208 930 4545
Fragments arranged by size:
4545 930 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2903 3787 4688 4950 820
Size: 197 926 960 884 901 262 1553
Fragments arranged by size:
1553 960 926 901 884 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 2974 1517
Size: 1457 4226
StyI C'CwwG_G
Cuts at: 513 1065 3122 513
Size: 552 2057 3074
Fragments arranged by size:
3074 2057 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 2291 2557 3710 3727 3880 4065
Size: 1255 588 123 266 1153 17 153 185
Cuts at: 4065 4510 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1153 588 445 266 185 153
123 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2538 2676 3929
Size: 274 80 33 51 1443 561 138 1253
Cuts at: 3929 5641 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1253 561 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2485
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 2078 3008 1870
Size: 208 930 4545
Fragments arranged by size:
4545 930 208
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI