Restriction map for
pFN21AB8950
(Circular) (Six-base) MAPSORT of: pFN21AB8950.seq Check: 2502 from: 1 to: 5677
pFN21AB8950 5677 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2487 2998 2487
Size: 511 5166
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 5124 5392 1632
Size: 2108 1384 268 1917
Fragments arranged by size:
2108 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 2608 3420 4992 2608
Size: 812 1572 3293
Fragments arranged by size:
3293 1572 812
ApaI G_GGCC'C
Cuts at: 2181 2181
Size: 5677
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2986 3212 3364 5473 1493
Size: 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2403 2921 2941 3002 1870
Size: 73 237 223 518 20 61 4545
Fragments arranged by size:
4545 518 237 223 73 61 20
AvrII C'CTAG_G
Cuts at: 2695 2695
Size: 5677
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 2487 2662 2707 2998
Size: 325 217 627 341 359 175 45 291
Cuts at: 2998 4453 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 359 341 325 291 217
175 45
BanII G_rGCy'C
Cuts at: 1391 2103 2181 2921 2996 1391
Size: 712 78 740 75 4072
Fragments arranged by size:
4072 740 712 78 75
BbeI G_GCGC'C
Cuts at: 2132 2711 2132
Size: 579 5098
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2381 928
Size: 971 482 4224
Fragments arranged by size:
4224 971 482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2171 2205 3868 3902 1903
Size: 34 234 34 1663 34 3678
Fragments arranged by size:
3678 1663 234 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2975 3569 4779 2975
Size: 594 1210 3873
Fragments arranged by size:
3873 1210 594
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2553 5672 2553
Size: 3119 2558
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 2181 3738 4894 1790
Size: 391 1557 1156 2573
Fragments arranged by size:
2573 1557 1156 391
BmgBI CAC'GTC
Cuts at: 1318 2538 1318
Size: 1220 4457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3408 4366 442
Size: 416 53 83 399 24 1991 958 1753
Fragments arranged by size:
1991 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2390 2483 2630 4337 1239
Size: 896 255 93 147 1707 2579
Fragments arranged by size:
2579 1707 896 255 147 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2288 3468 493
Size: 711 1084 1180 2702
Fragments arranged by size:
2702 1180 1084 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2129 2708 2892 3484
Size: 53 83 186 936 596 579 184 592
Cuts at: 3484 3866 4501 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 596 592 579 382 186
184 83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5452
Fragments arranged by size:
5452 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 1186
Size: 939 4738
BseYI C'CCAG_C
Cuts at: 2012 2174 2221 2308 2842 4880 5376 2012
Size: 162 47 87 534 2038 496 2313
Fragments arranged by size:
2313 2038 534 496 162 87 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2797 1163
Size: 573 1061 4043
Fragments arranged by size:
4043 1061 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 2436 3738 3823 4894 2103
Size: 333 1302 85 1071 2886
Fragments arranged by size:
2886 1302 1071 333 85
BsmI GAATG_Cn'
Cuts at: 2911 3188 3281 2911
Size: 277 93 5307
Fragments arranged by size:
5307 277 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2181 2436 2921 2996 3738 3823
Size: 399 313 78 255 485 75 742 85
Cuts at: 3823 4894 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 485 399 313 255 85
78 75
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2440 2536 3032 844
Size: 1596 96 496 3489
Fragments arranged by size:
3489 1596 496 96
BsrBI CCG'CTC
Cuts at: 1394 1823 2398 2467 3564 1394
Size: 429 575 69 1097 3507
Fragments arranged by size:
3507 1097 575 429 69
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2239 2568 2851 4321 4508 1700
Size: 110 429 329 283 1470 187 2869
Fragments arranged by size:
2869 1470 429 329 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 2212 2229 2346 1925
Size: 287 17 117 5256
Fragments arranged by size:
5256 287 117 17
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2691 794
Size: 1897 3780
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 2553 3007 3374 3759 3776 5228 5314 5326
Size: 581 454 367 385 17 1452 86 12
Cuts at: 5326 5672 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 581 454 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2694 487
Size: 683 56 1468 3470
Fragments arranged by size:
3470 1468 683 56
BtsI GCAGTG_nn'
Cuts at: 2814 2856 3193 3987 4007 2814
Size: 42 337 794 20 4484
Fragments arranged by size:
4484 794 337 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2241 2517
Size: 54 1425 50 275 18 318 93 276
Cuts at: 2517 2853 3124 4013 5642 8
Size: 336 271 889 1629 43
Fragments arranged by size:
1629 1425 889 336 318 276 275 271
93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 3607 1353
Size: 1088 1166 3423
Fragments arranged by size:
3423 1166 1088
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2399 2431 4264 4638 4784 387
Size: 1064 138 810 32 1833 374 146 1280
Fragments arranged by size:
1833 1280 1064 810 374 146 138 32
EcoICRI GAG'CTC
Cuts at: 2101 2101
Size: 5677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2390 2483 2630 3740 4337 5124
Size: 393 503 255 93 147 1110 597 787
Cuts at: 5124 5392 1239
Size: 268 1524
Fragments arranged by size:
1524 1110 787 597 503 393 268 255
147 93
EcoO109I rG'GnC_Cy
Cuts at: 2317 2317
Size: 5677
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2132 2473 2575 2711 4824 1678
Size: 454 341 102 136 2113 2531
Fragments arranged by size:
2531 2113 454 341 136 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4318 4350 4392 4424 1373
Size: 32 135 32 2746 32 42 32 2626
Fragments arranged by size:
2746 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2128 2707 2128
Size: 579 5098
KpnI G_GTAC'C
Cuts at: 2491 3002 2491
Size: 511 5166
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 10
Size: 54 1425 343 687 3168
Fragments arranged by size:
3168 1425 687 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3635 3994 4153 518
Size: 648 2469 359 159 2042
Fragments arranged by size:
2469 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2485 2515 2593 2811 3093 3770 4918 5163
Size: 879 30 78 218 282 677 1148 245
Cuts at: 5163 1606
Size: 2120
Fragments arranged by size:
2120 1148 879 677 282 245 218 78
30
NaeI GCC'GGC
Cuts at: 2853 2853
Size: 5677
NarI GG'CG_CC
Cuts at: 2129 2708 2129
Size: 579 5098
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NgoMIV G'CCGG_C
Cuts at: 2851 2851
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2146 3035 4580 1132
Size: 97 917 889 1545 2229
Fragments arranged by size:
2229 1545 917 889 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2608 1108
Size: 1500 4177
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PmlI CAC'GTG
Cuts at: 2288 2288
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2317 2317
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 2754 3247 2754
Size: 493 5184
PspOMI G'GGCC_C
Cuts at: 2177 2177
Size: 5677
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2558 2590 2558
Size: 32 5645
PstI C_TGCA'G
Cuts at: 830 2355 2798 3029 830
Size: 1525 443 231 3478
Fragments arranged by size:
3478 1525 443 231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2515 2811 2515
Size: 296 5381
SacI G_AGCT'C
Cuts at: 2103 2103
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SapI GCTCTTCn'nnn_
Cuts at: 2441 2441
Size: 5677
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 2293 2351 2794 2806 3025 4160 4841
Size: 219 1248 58 443 12 219 1135 681
Cuts at: 4841 5032 826
Size: 191 1471
Fragments arranged by size:
1471 1248 1135 681 443 219 219 191
58 12
SfoI GGC'GCC
Cuts at: 2130 2709 2130
Size: 579 5098
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 2405 2923 3004 1872
Size: 533 518 81 4545
Fragments arranged by size:
4545 533 518 81
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3781 4682 4944 820
Size: 197 926 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 2146 3035 1132
Size: 1014 889 3774
Fragments arranged by size:
3774 1014 889
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 2168 1517
Size: 651 5026
StyI C'CwwG_G
Cuts at: 513 1065 2313 2695 3116 513
Size: 552 1248 382 421 3074
Fragments arranged by size:
3074 1248 552 421 382
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3704 3721 3874 4059 4504 325
Size: 1255 2124 17 153 185 445 1498
Fragments arranged by size:
2124 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3923 5635 96
Size: 274 80 33 51 1443 1946 1712 138
Fragments arranged by size:
1946 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 2403 2921 3002 1870
Size: 533 518 81 4545
Fragments arranged by size:
4545 533 518 81
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI
XcmI