Restriction map for
pFN21AB8947
(Circular) (Six-base) MAPSORT of: pFN21AB8947.seq Check: 1029 from: 1 to: 5647
pFN21AB8947 5647 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 5094 5362 1632
Size: 2078 1384 268 1917
Fragments arranged by size:
2078 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2806 3390 4962 2806
Size: 584 1572 3491
Fragments arranged by size:
3491 1572 584
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2816 2968 4423 618
Size: 325 217 627 1029 152 1455 1842
Fragments arranged by size:
1842 1455 1029 627 325 217 152
BanII G_rGCy'C
Cuts at: 1391 2411 2454 2966 1391
Size: 1020 43 512 4072
Fragments arranged by size:
4072 1020 512 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2293 2926 928
Size: 971 394 633 3649
Fragments arranged by size:
3649 971 633 394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2699 2811 4258
Size: 107 122 71 631 605 1027 112 1447
Cuts at: 4258 136
Size: 1525
Fragments arranged by size:
1525 1447 1027 631 605 122 112 107
71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 2516 3064 2516
Size: 548 5099
Bme1580I G_kGCm'C
Cuts at: 1790 3708 4864 1790
Size: 1918 1156 2573
Fragments arranged by size:
2573 1918 1156
BmgBI CAC'GTC
Cuts at: 1318 2383 1318
Size: 1065 4582
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2327 2378 2861 2882 4307 1239
Size: 962 126 51 483 21 1425 2579
Fragments arranged by size:
2579 1425 962 483 126 51 21
Bpu10I CC'TnA_GC
Cuts at: 2228 2474 2228
Size: 246 5401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2870 2924 3772 4637 4935 2870
Size: 54 848 865 298 3582
Fragments arranged by size:
3582 865 848 298 54
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2354 4310 882
Size: 617 855 1956 2219
Fragments arranged by size:
2219 1956 855 617
BsaAI yAC'GTr
Cuts at: 493 1204 2178 2446 3438 493
Size: 711 974 268 992 2702
Fragments arranged by size:
2702 992 974 711 268
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2786 3454 3836 4471
Size: 53 83 186 936 1253 668 382 635
Cuts at: 4471 275
Size: 1451
Fragments arranged by size:
1451 1253 936 668 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2148 2178 2327 2357 1178
Size: 30 165 30 745 30 149 30 4468
Fragments arranged by size:
4468 745 165 149 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2172 2594 2879 1186
Size: 986 422 285 3954
Fragments arranged by size:
3954 986 422 285
BseYI C'CCAG_C
Cuts at: 2281 2468 2905 4850 5346 2281
Size: 187 437 1945 496 2582
Fragments arranged by size:
2582 1945 496 437 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 1163
Size: 573 765 4309
Fragments arranged by size:
4309 765 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3708 3793 4864 3708
Size: 85 1071 4491
Fragments arranged by size:
4491 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2411 2454 2966 3708 3793 4864 1391
Size: 399 621 43 512 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 621 512 399 85 43
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 2292 3536 5266 2292
Size: 1244 1730 2673
Fragments arranged by size:
2673 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3002 844
Size: 2158 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 2424 2494 1925
Size: 499 70 5078
Fragments arranged by size:
5078 499 70
BssSI C'ACGA_G
Cuts at: 2624 3707 4719 2624
Size: 1083 1012 3552
Fragments arranged by size:
3552 1083 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 2704 1184
Size: 1520 4127
BstXI CCAn_nnnn'nTGG
Cuts at: 2860 2860
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2130 2844 2977 3344 3729 3746 5198 5284
Size: 158 714 133 367 385 17 1452 86
Cuts at: 5284 5296 5642 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 714 385 367 346 158 133
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2455 2461 2640 513
Size: 552 939 451 6 179 3520
Fragments arranged by size:
3520 939 552 451 179 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2102 487
Size: 683 56 876 4032
Fragments arranged by size:
4032 876 683 56
BtsI GCAGTG_nn'
Cuts at: 3163 3957 3977 3163
Size: 794 20 4833
Fragments arranged by size:
4833 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2172 2236
Size: 54 1425 50 275 18 192 150 64
Cuts at: 2236 2262 2458 2616 2637 2691 2850 2922 3094
Size: 26 196 158 21 54 159 72 172
Cuts at: 3094 3983 5612 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 275 196 192 172 159
158 150 72 64 54 54 50 43
26 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 2864 2900 3577 1353
Size: 1478 33 36 677 3423
Fragments arranged by size:
3423 1478 677 36 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2327 2378 2861 2882 3710 4307
Size: 393 569 126 51 483 21 828 597
Cuts at: 4307 5094 5362 1239
Size: 787 268 1524
Fragments arranged by size:
1524 828 787 597 569 483 393 268
126 51 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2576 2852 2576
Size: 276 5371
EcoO109I rG'GnC_Cy
Cuts at: 2224 2485 2557 2803 2890 2224
Size: 261 72 246 87 4981
Fragments arranged by size:
4981 261 246 87 72
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2911 2362
Size: 549 5098
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2718 2763 4794 1678
Size: 1040 45 2031 2531
Fragments arranged by size:
2531 2031 1040 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2122 2154 2327 2359 2863
Size: 32 135 32 550 32 173 32 504
Cuts at: 2863 2895 4288 4320 4362 4394 1373
Size: 32 1393 32 42 32 2626
Fragments arranged by size:
2626 1393 550 504 173 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 2174 2852 10
Size: 54 1425 343 192 150 678 2805
Fragments arranged by size:
2805 1425 678 343 192 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 3605 3964 4123 518
Size: 648 1491 948 359 159 2042
Fragments arranged by size:
2042 1491 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2201 2260 2314 3063 3740 4888 5133 1606
Size: 595 59 54 749 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 595 245 59 54
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2566 2647 2728 2764 1348
Size: 1218 81 81 36 4231
Fragments arranged by size:
4231 1218 81 81 36
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PfoI T'CCnGG_A
Cuts at: 2304 2838 2304
Size: 534 5113
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PmlI CAC'GTG
Cuts at: 2178 2178
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2890 2224
Size: 666 4981
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2577 2802 2999 830
Size: 1747 225 197 3478
Fragments arranged by size:
3478 1747 225 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
PvuII CAG'CTG
Cuts at: 2314 2314
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2577 2999 2577
Size: 422 5225
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2573 2798 2995 4130 4811 5002 826
Size: 219 1528 225 197 1135 681 191 1471
Fragments arranged by size:
1528 1471 1135 681 225 219 197 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2885 2939 3751 4652 4914 820
Size: 197 926 942 54 812 901 262 1553
Fragments arranged by size:
1553 942 926 901 812 262 197 54
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2004 2853 3086 513
Size: 552 939 849 233 3074
Fragments arranged by size:
3074 939 849 552 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2536 3674 3691 3844 4029 4474 325
Size: 1255 956 1138 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1138 956 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3893 5605 96
Size: 274 80 33 51 1443 1916 1712 138
Fragments arranged by size:
1916 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2148 2180 2367 2399 5607 5639 2148
Size: 32 187 32 3208 32 2156
Fragments arranged by size:
3208 2156 187 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2725 2278
Size: 447 5200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI