Restriction map for
pFN21AB8946
(Circular) (Six-base) MAPSORT of: pFN21AB8946.seq Check: 7899 from: 1 to: 5644
pFN21AB8946 5644 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2346 2796 2987 1937
Size: 409 450 191 4594
Fragments arranged by size:
4594 450 409 191
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2173 2773 3707 5091 5359 1632
Size: 541 600 934 1384 268 1917
Fragments arranged by size:
1917 1384 934 600 541 268
AfeI AGC'GCT
Cuts at: 2738 2738
Size: 5644
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2040 4543 5520 1203
Size: 837 2503 977 1327
Fragments arranged by size:
2503 1327 977 837
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 5612
AlwNI CAG_nnn'CTG
Cuts at: 2280 3387 4959 2280
Size: 1107 1572 2965
Fragments arranged by size:
2965 1572 1107
ApaI G_GGCC'C
Cuts at: 2511 2511
Size: 5644
ApaLI G'TGCA_C
Cuts at: 2270 3701 4857 2270
Size: 1431 1156 3057
Fragments arranged by size:
3057 1431 1156
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AscI GG'CGCG_CC
Cuts at: 2559 2559
Size: 5644
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2483 2651 2669 2969 1870
Size: 73 128 412 168 18 300 4545
Fragments arranged by size:
4545 412 300 168 128 73 18
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2223 2290 2450 2646 2965
Size: 325 217 627 436 67 160 196 319
Cuts at: 2965 4420 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 436 325 319 217 196
160 67
BanII G_rGCy'C
Cuts at: 1391 2211 2268 2336 2511 2963 1391
Size: 820 57 68 175 452 4072
Fragments arranged by size:
4072 820 452 175 68 57
BbeI G_GCGC'C
Cuts at: 2294 2650 2294
Size: 356 5288
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 2670 928
Size: 971 213 558 3902
Fragments arranged by size:
3902 971 558 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2511 3705 4861 1790
Size: 484 237 1194 1156 2573
Fragments arranged by size:
2573 1194 1156 484 237
BmgBI CAC'GTC
Cuts at: 1318 2320 1318
Size: 1002 4642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2695 4304 1239
Size: 832 624 1609 2579
Fragments arranged by size:
2579 1609 832 624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2901 4307 882
Size: 617 1402 1406 2219
Fragments arranged by size:
2219 1406 1402 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2291 2406 2647 3451
Size: 53 83 186 936 758 115 241 804
Cuts at: 3451 3833 4468 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 804 758 635 382 241 186
115 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 2237 2267 2453
Size: 30 165 30 726 30 78 30 186
Cuts at: 2453 2483 1178
Size: 30 4339
Fragments arranged by size:
4339 726 186 165 78 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2137 2276 2461 2642 2930 1186
Size: 837 114 139 185 181 288 3900
Fragments arranged by size:
3900 837 288 185 181 139 114
BseYI C'CCAG_C
Cuts at: 2294 4847 5343 2294
Size: 2553 496 2595
Fragments arranged by size:
2595 2553 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2304 3094 3855 4883 1540
Size: 275 186 303 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 303 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 2274 2336 3705 3790 4861 2268
Size: 6 62 1369 85 1071 3051
Fragments arranged by size:
3051 1369 1071 85 62 6
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
BsmBI CGTCTCn'nnnn_
Cuts at: 2074 2603 2074
Size: 529 5115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2268 2274 2336 2511 2963 3705
Size: 399 421 57 6 62 175 452 742
Cuts at: 3705 3790 4861 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 421 399 175 85
62 57 6
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2999 844
Size: 1319 836 3489
Fragments arranged by size:
3489 1319 836
BsrBI CCG'CTC
Cuts at: 1394 1823 2638 3531 1394
Size: 429 815 893 3507
Fragments arranged by size:
3507 893 815 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2303 2563 2702 4288 4475 1700
Size: 110 493 260 139 1586 187 2869
Fragments arranged by size:
2869 1586 493 260 187 139 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 2559 1925
Size: 634 5010
BssSI C'ACGA_G
Cuts at: 2267 3704 4716 2267
Size: 1437 1012 3195
Fragments arranged by size:
3195 1437 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2760 2974 3341 3726 3743 5195 5281 5293
Size: 788 214 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 788 385 367 346 214 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2230 2256 2391 2520 2532 513
Size: 552 939 226 26 135 129 12 3625
Fragments arranged by size:
3625 939 552 226 135 129 26 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3160 3954 3974 2245
Size: 915 794 20 3915
Fragments arranged by size:
3915 915 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2325 2337
Size: 54 1425 50 275 18 471 24 12
Cuts at: 2337 2517 2683 2716 3091 3980 5609 8
Size: 180 166 33 375 889 1629 43
Fragments arranged by size:
1629 1425 889 471 375 275 180 166
54 50 43 33 24 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2301 3091 1537
Size: 275 489 790 4090
Fragments arranged by size:
4090 790 489 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2266 2334 2266
Size: 68 5576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2173 2695 2773 3707 4304 5091
Size: 393 439 102 522 78 934 597 787
Cuts at: 5091 5359 1239
Size: 268 1524
Fragments arranged by size:
1524 934 787 597 522 439 393 268
102 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2414 2159
Size: 255 5389
EcoO109I rG'GnC_Cy
Cuts at: 2507 2508 2507
Size: 1 5643
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2076 2076
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2294 2499 2650 2740 2818 4791 1678
Size: 616 205 151 90 78 1973 2531
Fragments arranged by size:
2531 1973 616 205 151 90 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373
Size: 32 135 32 2713 32 42 32 2626
Fragments arranged by size:
2713 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2290 2646 2290
Size: 356 5288
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2175 2191 2499 4758 4942 1305
Size: 870 16 308 2259 184 2007
Fragments arranged by size:
2259 2007 870 308 184 16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 2718 10
Size: 54 1425 343 495 391 2936
Fragments arranged by size:
2936 1425 495 391 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3602 3961 4120 518
Size: 648 2436 359 159 2042
Fragments arranged by size:
2436 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2232 2258 2534 2586 3060 3737 4885 5130
Size: 626 26 276 52 474 677 1148 245
Cuts at: 5130 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 626 474 276 245 52
26
NaeI GCC'GGC
Cuts at: 2305 2565 2704 2305
Size: 260 139 5245
Fragments arranged by size:
5245 260 139
NarI GG'CG_CC
Cuts at: 2291 2647 2291
Size: 356 5288
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4153
Fragments arranged by size:
4153 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NgoMIV G'CCGG_C
Cuts at: 2303 2563 2702 2303
Size: 260 139 5245
Fragments arranged by size:
5245 260 139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2044 2836 3002 4547 1132
Size: 97 815 792 166 1545 2229
Fragments arranged by size:
2229 1545 815 792 166 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 2040 4543 2040
Size: 2503 3141
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2220 1108
Size: 1112 4532
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2507 2507
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2289 2996 830
Size: 1459 707 3478
Fragments arranged by size:
3478 1459 707
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SacI G_AGCT'C
Cuts at: 2268 2336 2268
Size: 68 5576
SacII CC_GC'GG
Cuts at: 2233 2259 2535 2233
Size: 26 276 5342
Fragments arranged by size:
5342 276 26
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2289 2996 2289
Size: 707 4937
ScaI AGT'ACT
Cuts at: 2921 3892 5604 2921
Size: 971 1712 2961
Fragments arranged by size:
2961 1712 971
SfcI C'TryA_G
Cuts at: 826 1045 2285 2383 2992 4127 4808 4999 826
Size: 219 1240 98 609 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 681 609 219 191 98
SfoI GGC'GCC
Cuts at: 2292 2648 2292
Size: 356 5288
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2485 2971 1872
Size: 613 486 4545
Fragments arranged by size:
4545 613 486
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3748 4649 4911 820
Size: 197 926 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 2836 3002 1132
Size: 1704 166 3774
Fragments arranged by size:
3774 1704 166
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2004 3083 513
Size: 552 939 1079 3074
Fragments arranged by size:
3074 1079 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2057 2919 3890
Size: 274 80 33 51 1443 80 862 971
Cuts at: 3890 5602 96
Size: 1712 138
Fragments arranged by size:
1712 1443 971 862 274 138 80 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 5604 5636 2237
Size: 32 3335 32 2245
Fragments arranged by size:
3335 2245 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2116 1316
Size: 800 4844
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2483 2969 1870
Size: 613 486 4545
Fragments arranged by size:
4545 613 486
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI